1x complete mini roche cocktail protease inhibitor  (Roche)

 
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    Structured Review

    Roche 1x complete mini roche cocktail protease inhibitor
    1x Complete Mini Roche Cocktail Protease Inhibitor, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1x complete mini roche cocktail protease inhibitor/product/Roche
    Average 92 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    1x complete mini roche cocktail protease inhibitor - by Bioz Stars, 2020-04
    92/100 stars

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    Related Articles

    Nucleic Acid Electrophoresis:

    Article Title: The Interplay between Cyclic AMP, MAPK, and NF-κB Pathways in Response to Proinflammatory Signals in Microglia
    Article Snippet: The nuclear pellet was suspended in 50 μ L of a hypertonic buffer composed of HEPES (20 mM, pH 7.9), glycerol (25%), NaCl (500 mM), MgCl2 (1.5 mM), EDTA (0.2 mM), DTT (1 mM), Na3 VO4 (1 mM), NaF (10 mM), Na4 P2 O7 (2 mM), and 1X Complete Mini Roche Cocktail Protease Inhibitor and incubated for 30 min on ice followed with centrifugation for 10 min at 14,000 rpm. .. A total of 20 μ g of protein was loaded into each well of a polyacrylamide gel that was subjected to denaturing gel electrophoresis and subsequently transferred onto nitrocellulose membranes (Bio-Rad Laboratories) as described elsewhere [ ].

    Article Title: Pro-inflammatory cytokine regulation of cyclic AMP-phosphodiesterase 4 signaling in microglia in vitro and following CNS injury
    Article Snippet: Briefly, microglia were treated with ice-cold lysis buffer: 15mM Tris pH 7.6, 0.25M sucrose, 1mM MgCl2 , 1mM EGTA, 1mM DTT, 1.25μg/ml pepstatin A, 10μg/ml leupeptin, 25μg/ml aprotinin, 1mM benzamidine, 0.5mM PMSF, 1mM Na3 VO4 , 50mM NaF, 2mM Na4 P2 O7 , and 1X Complete Mini Roche cocktail protease inhibitor (Roche, Indianapolis, IN). .. Then equal amounts of protein (30μg/lane) from each sample underwent gel electrophoresis (10% SDS-PAGE) and were transferred to Polyvinylidene fluoride (PVDF) membranes.

    Centrifugation:

    Article Title: The Interplay between Cyclic AMP, MAPK, and NF-κB Pathways in Response to Proinflammatory Signals in Microglia
    Article Snippet: The cell pellet was then resuspended in 250 μ L of ice-cold 1X hypotonic buffer consisting of HEPES (10 mM, pH 7.9), MgCl2 (1.5 mM), KCl (10 mM), EDTA (0.1 mM), DTT (1 mM), 0.1% Nonidet P40, pepstatin A (1.25 μ g/mL), leupeptin (10 μ g/mL), aprotinin (25 μ g/mL), benzamidine (1 mM), PMSF (1 mM), Na3 VO4 (2 mM), NaF (10 mM), Na4 P2 O7 (2 mM), and 1X Complete Mini Roche Cocktail Protease Inhibitor (Roche) and then incubated for 30 min on ice. .. The nuclear pellet was suspended in 50 μ L of a hypertonic buffer composed of HEPES (20 mM, pH 7.9), glycerol (25%), NaCl (500 mM), MgCl2 (1.5 mM), EDTA (0.2 mM), DTT (1 mM), Na3 VO4 (1 mM), NaF (10 mM), Na4 P2 O7 (2 mM), and 1X Complete Mini Roche Cocktail Protease Inhibitor and incubated for 30 min on ice followed with centrifugation for 10 min at 14,000 rpm.

    Article Title: The Interplay between Cyclic AMP, MAPK, and NF-κB Pathways in Response to Proinflammatory Signals in Microglia
    Article Snippet: .. The nuclear pellet was suspended in 50 μ L of a hypertonic buffer composed of HEPES (20 mM, pH 7.9), glycerol (25%), NaCl (500 mM), MgCl2 (1.5 mM), EDTA (0.2 mM), DTT (1 mM), Na3 VO4 (1 mM), NaF (10 mM), Na4 P2 O7 (2 mM), and 1X Complete Mini Roche Cocktail Protease Inhibitor and incubated for 30 min on ice followed with centrifugation for 10 min at 14,000 rpm. .. The supernatant constituting the nuclear fraction was analyzed for protein concentration using the BCA protein assay according to the manufacturer's instructions (Bio-Rad Laboratories).

    Concentration Assay:

    Article Title: The Interplay between Cyclic AMP, MAPK, and NF-κB Pathways in Response to Proinflammatory Signals in Microglia
    Article Snippet: The nuclear pellet was suspended in 50 μ L of a hypertonic buffer composed of HEPES (20 mM, pH 7.9), glycerol (25%), NaCl (500 mM), MgCl2 (1.5 mM), EDTA (0.2 mM), DTT (1 mM), Na3 VO4 (1 mM), NaF (10 mM), Na4 P2 O7 (2 mM), and 1X Complete Mini Roche Cocktail Protease Inhibitor and incubated for 30 min on ice followed with centrifugation for 10 min at 14,000 rpm. .. The lysates were normalized to a final concentration of 1 mg/mL and boiled at 100°C for 20 min in Laemmli buffer (Bio-Rad Laboratories).

    Protease Inhibitor:

    Article Title: The Interplay between Cyclic AMP, MAPK, and NF-κB Pathways in Response to Proinflammatory Signals in Microglia
    Article Snippet: .. For preparation of whole cell lysates, EOC2 microglia were grown on 100 mm plates, subjected to different experimental conditions, and then incubated for 15 min in 0.5 mL ice-cold lysis buffer (Cell Signaling Technology Inc.), containing pepstatin A (1.25 μ g/mL), leupeptin (10 μ g/mL), aprotinin (25 μ g/mL), benzamidine (1 mM), PMSF (0.5 mM), Na3 VO4 (1 mM), NaF (50 mM), Na4 P2 O7 (2 mM), and 1X Complete Mini Roche Cocktail Protease Inhibitor (Roche, Indianapolis, IN). ..

    Article Title: The Interplay between Cyclic AMP, MAPK, and NF-κB Pathways in Response to Proinflammatory Signals in Microglia
    Article Snippet: .. The cell pellet was then resuspended in 250 μ L of ice-cold 1X hypotonic buffer consisting of HEPES (10 mM, pH 7.9), MgCl2 (1.5 mM), KCl (10 mM), EDTA (0.1 mM), DTT (1 mM), 0.1% Nonidet P40, pepstatin A (1.25 μ g/mL), leupeptin (10 μ g/mL), aprotinin (25 μ g/mL), benzamidine (1 mM), PMSF (1 mM), Na3 VO4 (2 mM), NaF (10 mM), Na4 P2 O7 (2 mM), and 1X Complete Mini Roche Cocktail Protease Inhibitor (Roche) and then incubated for 30 min on ice. ..

    Article Title: The Interplay between Cyclic AMP, MAPK, and NF-κB Pathways in Response to Proinflammatory Signals in Microglia
    Article Snippet: .. The nuclear pellet was suspended in 50 μ L of a hypertonic buffer composed of HEPES (20 mM, pH 7.9), glycerol (25%), NaCl (500 mM), MgCl2 (1.5 mM), EDTA (0.2 mM), DTT (1 mM), Na3 VO4 (1 mM), NaF (10 mM), Na4 P2 O7 (2 mM), and 1X Complete Mini Roche Cocktail Protease Inhibitor and incubated for 30 min on ice followed with centrifugation for 10 min at 14,000 rpm. .. The supernatant constituting the nuclear fraction was analyzed for protein concentration using the BCA protein assay according to the manufacturer's instructions (Bio-Rad Laboratories).

    Article Title: Pro-inflammatory cytokine regulation of cyclic AMP-phosphodiesterase 4 signaling in microglia in vitro and following CNS injury
    Article Snippet: .. Briefly, microglia were treated with ice-cold lysis buffer: 15mM Tris pH 7.6, 0.25M sucrose, 1mM MgCl2 , 1mM EGTA, 1mM DTT, 1.25μg/ml pepstatin A, 10μg/ml leupeptin, 25μg/ml aprotinin, 1mM benzamidine, 0.5mM PMSF, 1mM Na3 VO4 , 50mM NaF, 2mM Na4 P2 O7 , and 1X Complete Mini Roche cocktail protease inhibitor (Roche, Indianapolis, IN). .. Next, lysates were assayed for total protein concentration using the Coomassie Plus protein assay (Bio-Rad Laboratories, Hercules, CA).

    BIA-KA:

    Article Title: The Interplay between Cyclic AMP, MAPK, and NF-κB Pathways in Response to Proinflammatory Signals in Microglia
    Article Snippet: The cell pellet was then resuspended in 250 μ L of ice-cold 1X hypotonic buffer consisting of HEPES (10 mM, pH 7.9), MgCl2 (1.5 mM), KCl (10 mM), EDTA (0.1 mM), DTT (1 mM), 0.1% Nonidet P40, pepstatin A (1.25 μ g/mL), leupeptin (10 μ g/mL), aprotinin (25 μ g/mL), benzamidine (1 mM), PMSF (1 mM), Na3 VO4 (2 mM), NaF (10 mM), Na4 P2 O7 (2 mM), and 1X Complete Mini Roche Cocktail Protease Inhibitor (Roche) and then incubated for 30 min on ice. .. The cell pellet was then resuspended in 250 μ L of ice-cold 1X hypotonic buffer consisting of HEPES (10 mM, pH 7.9), MgCl2 (1.5 mM), KCl (10 mM), EDTA (0.1 mM), DTT (1 mM), 0.1% Nonidet P40, pepstatin A (1.25 μ g/mL), leupeptin (10 μ g/mL), aprotinin (25 μ g/mL), benzamidine (1 mM), PMSF (1 mM), Na3 VO4 (2 mM), NaF (10 mM), Na4 P2 O7 (2 mM), and 1X Complete Mini Roche Cocktail Protease Inhibitor (Roche) and then incubated for 30 min on ice.

    Activation Assay:

    Article Title: The Interplay between Cyclic AMP, MAPK, and NF-κB Pathways in Response to Proinflammatory Signals in Microglia
    Article Snippet: Western Blotting Immunoblotting was performed on EOC2 microglial cell whole cell lysates or nuclear fractions to identify and quantify the expression and phosphorylation levels of various target proteins altered upon activation with TNF-α . .. For preparation of whole cell lysates, EOC2 microglia were grown on 100 mm plates, subjected to different experimental conditions, and then incubated for 15 min in 0.5 mL ice-cold lysis buffer (Cell Signaling Technology Inc.), containing pepstatin A (1.25 μ g/mL), leupeptin (10 μ g/mL), aprotinin (25 μ g/mL), benzamidine (1 mM), PMSF (0.5 mM), Na3 VO4 (1 mM), NaF (50 mM), Na4 P2 O7 (2 mM), and 1X Complete Mini Roche Cocktail Protease Inhibitor (Roche, Indianapolis, IN).

    Incubation:

    Article Title: The Interplay between Cyclic AMP, MAPK, and NF-κB Pathways in Response to Proinflammatory Signals in Microglia
    Article Snippet: .. For preparation of whole cell lysates, EOC2 microglia were grown on 100 mm plates, subjected to different experimental conditions, and then incubated for 15 min in 0.5 mL ice-cold lysis buffer (Cell Signaling Technology Inc.), containing pepstatin A (1.25 μ g/mL), leupeptin (10 μ g/mL), aprotinin (25 μ g/mL), benzamidine (1 mM), PMSF (0.5 mM), Na3 VO4 (1 mM), NaF (50 mM), Na4 P2 O7 (2 mM), and 1X Complete Mini Roche Cocktail Protease Inhibitor (Roche, Indianapolis, IN). ..

    Article Title: The Interplay between Cyclic AMP, MAPK, and NF-κB Pathways in Response to Proinflammatory Signals in Microglia
    Article Snippet: .. The cell pellet was then resuspended in 250 μ L of ice-cold 1X hypotonic buffer consisting of HEPES (10 mM, pH 7.9), MgCl2 (1.5 mM), KCl (10 mM), EDTA (0.1 mM), DTT (1 mM), 0.1% Nonidet P40, pepstatin A (1.25 μ g/mL), leupeptin (10 μ g/mL), aprotinin (25 μ g/mL), benzamidine (1 mM), PMSF (1 mM), Na3 VO4 (2 mM), NaF (10 mM), Na4 P2 O7 (2 mM), and 1X Complete Mini Roche Cocktail Protease Inhibitor (Roche) and then incubated for 30 min on ice. ..

    Article Title: The Interplay between Cyclic AMP, MAPK, and NF-κB Pathways in Response to Proinflammatory Signals in Microglia
    Article Snippet: .. The nuclear pellet was suspended in 50 μ L of a hypertonic buffer composed of HEPES (20 mM, pH 7.9), glycerol (25%), NaCl (500 mM), MgCl2 (1.5 mM), EDTA (0.2 mM), DTT (1 mM), Na3 VO4 (1 mM), NaF (10 mM), Na4 P2 O7 (2 mM), and 1X Complete Mini Roche Cocktail Protease Inhibitor and incubated for 30 min on ice followed with centrifugation for 10 min at 14,000 rpm. .. The supernatant constituting the nuclear fraction was analyzed for protein concentration using the BCA protein assay according to the manufacturer's instructions (Bio-Rad Laboratories).

    Expressing:

    Article Title: The Interplay between Cyclic AMP, MAPK, and NF-κB Pathways in Response to Proinflammatory Signals in Microglia
    Article Snippet: Western Blotting Immunoblotting was performed on EOC2 microglial cell whole cell lysates or nuclear fractions to identify and quantify the expression and phosphorylation levels of various target proteins altered upon activation with TNF-α . .. For preparation of whole cell lysates, EOC2 microglia were grown on 100 mm plates, subjected to different experimental conditions, and then incubated for 15 min in 0.5 mL ice-cold lysis buffer (Cell Signaling Technology Inc.), containing pepstatin A (1.25 μ g/mL), leupeptin (10 μ g/mL), aprotinin (25 μ g/mL), benzamidine (1 mM), PMSF (0.5 mM), Na3 VO4 (1 mM), NaF (50 mM), Na4 P2 O7 (2 mM), and 1X Complete Mini Roche Cocktail Protease Inhibitor (Roche, Indianapolis, IN).

    Protein Concentration:

    Article Title: The Interplay between Cyclic AMP, MAPK, and NF-κB Pathways in Response to Proinflammatory Signals in Microglia
    Article Snippet: The cell pellet was then resuspended in 250 μ L of ice-cold 1X hypotonic buffer consisting of HEPES (10 mM, pH 7.9), MgCl2 (1.5 mM), KCl (10 mM), EDTA (0.1 mM), DTT (1 mM), 0.1% Nonidet P40, pepstatin A (1.25 μ g/mL), leupeptin (10 μ g/mL), aprotinin (25 μ g/mL), benzamidine (1 mM), PMSF (1 mM), Na3 VO4 (2 mM), NaF (10 mM), Na4 P2 O7 (2 mM), and 1X Complete Mini Roche Cocktail Protease Inhibitor (Roche) and then incubated for 30 min on ice. .. The cell pellet was then resuspended in 250 μ L of ice-cold 1X hypotonic buffer consisting of HEPES (10 mM, pH 7.9), MgCl2 (1.5 mM), KCl (10 mM), EDTA (0.1 mM), DTT (1 mM), 0.1% Nonidet P40, pepstatin A (1.25 μ g/mL), leupeptin (10 μ g/mL), aprotinin (25 μ g/mL), benzamidine (1 mM), PMSF (1 mM), Na3 VO4 (2 mM), NaF (10 mM), Na4 P2 O7 (2 mM), and 1X Complete Mini Roche Cocktail Protease Inhibitor (Roche) and then incubated for 30 min on ice.

    Article Title: Pro-inflammatory cytokine regulation of cyclic AMP-phosphodiesterase 4 signaling in microglia in vitro and following CNS injury
    Article Snippet: Briefly, microglia were treated with ice-cold lysis buffer: 15mM Tris pH 7.6, 0.25M sucrose, 1mM MgCl2 , 1mM EGTA, 1mM DTT, 1.25μg/ml pepstatin A, 10μg/ml leupeptin, 25μg/ml aprotinin, 1mM benzamidine, 0.5mM PMSF, 1mM Na3 VO4 , 50mM NaF, 2mM Na4 P2 O7 , and 1X Complete Mini Roche cocktail protease inhibitor (Roche, Indianapolis, IN). .. Next, lysates were assayed for total protein concentration using the Coomassie Plus protein assay (Bio-Rad Laboratories, Hercules, CA).

    Western Blot:

    Article Title: The Interplay between Cyclic AMP, MAPK, and NF-κB Pathways in Response to Proinflammatory Signals in Microglia
    Article Snippet: Paragraph title: 2.4.3. Western Blotting ... For preparation of whole cell lysates, EOC2 microglia were grown on 100 mm plates, subjected to different experimental conditions, and then incubated for 15 min in 0.5 mL ice-cold lysis buffer (Cell Signaling Technology Inc.), containing pepstatin A (1.25 μ g/mL), leupeptin (10 μ g/mL), aprotinin (25 μ g/mL), benzamidine (1 mM), PMSF (0.5 mM), Na3 VO4 (1 mM), NaF (50 mM), Na4 P2 O7 (2 mM), and 1X Complete Mini Roche Cocktail Protease Inhibitor (Roche, Indianapolis, IN).

    Article Title: Pro-inflammatory cytokine regulation of cyclic AMP-phosphodiesterase 4 signaling in microglia in vitro and following CNS injury
    Article Snippet: Paragraph title: Western Blot Analysis ... Briefly, microglia were treated with ice-cold lysis buffer: 15mM Tris pH 7.6, 0.25M sucrose, 1mM MgCl2 , 1mM EGTA, 1mM DTT, 1.25μg/ml pepstatin A, 10μg/ml leupeptin, 25μg/ml aprotinin, 1mM benzamidine, 0.5mM PMSF, 1mM Na3 VO4 , 50mM NaF, 2mM Na4 P2 O7 , and 1X Complete Mini Roche cocktail protease inhibitor (Roche, Indianapolis, IN).

    Lysis:

    Article Title: The Interplay between Cyclic AMP, MAPK, and NF-κB Pathways in Response to Proinflammatory Signals in Microglia
    Article Snippet: .. For preparation of whole cell lysates, EOC2 microglia were grown on 100 mm plates, subjected to different experimental conditions, and then incubated for 15 min in 0.5 mL ice-cold lysis buffer (Cell Signaling Technology Inc.), containing pepstatin A (1.25 μ g/mL), leupeptin (10 μ g/mL), aprotinin (25 μ g/mL), benzamidine (1 mM), PMSF (0.5 mM), Na3 VO4 (1 mM), NaF (50 mM), Na4 P2 O7 (2 mM), and 1X Complete Mini Roche Cocktail Protease Inhibitor (Roche, Indianapolis, IN). ..

    Article Title: The Interplay between Cyclic AMP, MAPK, and NF-κB Pathways in Response to Proinflammatory Signals in Microglia
    Article Snippet: For preparation of whole cell lysates, EOC2 microglia were grown on 100 mm plates, subjected to different experimental conditions, and then incubated for 15 min in 0.5 mL ice-cold lysis buffer (Cell Signaling Technology Inc.), containing pepstatin A (1.25 μ g/mL), leupeptin (10 μ g/mL), aprotinin (25 μ g/mL), benzamidine (1 mM), PMSF (0.5 mM), Na3 VO4 (1 mM), NaF (50 mM), Na4 P2 O7 (2 mM), and 1X Complete Mini Roche Cocktail Protease Inhibitor (Roche, Indianapolis, IN). .. The cell pellet was then resuspended in 250 μ L of ice-cold 1X hypotonic buffer consisting of HEPES (10 mM, pH 7.9), MgCl2 (1.5 mM), KCl (10 mM), EDTA (0.1 mM), DTT (1 mM), 0.1% Nonidet P40, pepstatin A (1.25 μ g/mL), leupeptin (10 μ g/mL), aprotinin (25 μ g/mL), benzamidine (1 mM), PMSF (1 mM), Na3 VO4 (2 mM), NaF (10 mM), Na4 P2 O7 (2 mM), and 1X Complete Mini Roche Cocktail Protease Inhibitor (Roche) and then incubated for 30 min on ice.

    Article Title: Pro-inflammatory cytokine regulation of cyclic AMP-phosphodiesterase 4 signaling in microglia in vitro and following CNS injury
    Article Snippet: .. Briefly, microglia were treated with ice-cold lysis buffer: 15mM Tris pH 7.6, 0.25M sucrose, 1mM MgCl2 , 1mM EGTA, 1mM DTT, 1.25μg/ml pepstatin A, 10μg/ml leupeptin, 25μg/ml aprotinin, 1mM benzamidine, 0.5mM PMSF, 1mM Na3 VO4 , 50mM NaF, 2mM Na4 P2 O7 , and 1X Complete Mini Roche cocktail protease inhibitor (Roche, Indianapolis, IN). .. Next, lysates were assayed for total protein concentration using the Coomassie Plus protein assay (Bio-Rad Laboratories, Hercules, CA).

    SDS Page:

    Article Title: Pro-inflammatory cytokine regulation of cyclic AMP-phosphodiesterase 4 signaling in microglia in vitro and following CNS injury
    Article Snippet: Briefly, microglia were treated with ice-cold lysis buffer: 15mM Tris pH 7.6, 0.25M sucrose, 1mM MgCl2 , 1mM EGTA, 1mM DTT, 1.25μg/ml pepstatin A, 10μg/ml leupeptin, 25μg/ml aprotinin, 1mM benzamidine, 0.5mM PMSF, 1mM Na3 VO4 , 50mM NaF, 2mM Na4 P2 O7 , and 1X Complete Mini Roche cocktail protease inhibitor (Roche, Indianapolis, IN). .. Then equal amounts of protein (30μg/lane) from each sample underwent gel electrophoresis (10% SDS-PAGE) and were transferred to Polyvinylidene fluoride (PVDF) membranes.

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  • 92
    Roche 1x complete mini roche cocktail protease inhibitor
    1x Complete Mini Roche Cocktail Protease Inhibitor, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1x complete mini roche cocktail protease inhibitor/product/Roche
    Average 92 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
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