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Promega 1ul rnasin
1ul Rnasin, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/1ul rnasin/product/Promega
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
1ul rnasin - by Bioz Stars, 2020-04
86/100 stars

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5x mmlv reverse transcriptase buffer
dnase
total rna

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Related Articles

RNA Sequencing Assay:

Article Title: A New Method for Stranded Whole Transcriptome RNA-seq
Article Snippet: Starting with 2 μg total RNA derived from cells, combine with 1.5 μL DNase (1.5 U, Promega Corporation, 2800 Woods Hollow Rd, Madison, WI 53711, cat. no. M198A), 3 μL 5X MMLV reverse transcriptase buffer (Promega supplied), 1uL RNasin (Promega cat. no. N251B) and the volume was increased to 15 μL with DIW. .. Prior to qRT-PCR, the cDNA was diluted with 160 μL DIW, and the sample was stored at −20°C. qRT-PCR was carried out as described in sec. 2.12 except that 5 μL of diluted cDNA was used instead of diluted RNA-seq PCR1.

Incubation:

Article Title: A New Method for Stranded Whole Transcriptome RNA-seq
Article Snippet: Starting with 2 μg total RNA derived from cells, combine with 1.5 μL DNase (1.5 U, Promega Corporation, 2800 Woods Hollow Rd, Madison, WI 53711, cat. no. M198A), 3 μL 5X MMLV reverse transcriptase buffer (Promega supplied), 1uL RNasin (Promega cat. no. N251B) and the volume was increased to 15 μL with DIW. .. The DNase reaction was incubated for 60 min at 37°C and then inactivated with DNase at 80°C for 5 min. cDNA was made by adding 2 μL/1μg random hexamer (Qiagen Sciences, Valencia, CA 91355, cat. no. 79236) and denatured at 70°C for 2 min. Then, the DNase-treated RNA was combined with 5 μL 5X MMLV reverse transcriptase buffer (Promega supplied), 2 μL 10 mM dNTP mix (Promega cat. no. U1511), 1 μL RNasin (Promega cat. no. N251B), 1.6 μL MMLV Reverse Transcriptase (Promega cat. no. M1708), 13.4 μL DIW and incubated at 42°C for 60 min, then the cDNA was denatured at 95°C for 5 min.

Quantitative RT-PCR:

Article Title: A New Method for Stranded Whole Transcriptome RNA-seq
Article Snippet: Paragraph title: 2.13 qRT-PCR with cDNA from total RNA ... Starting with 2 μg total RNA derived from cells, combine with 1.5 μL DNase (1.5 U, Promega Corporation, 2800 Woods Hollow Rd, Madison, WI 53711, cat. no. M198A), 3 μL 5X MMLV reverse transcriptase buffer (Promega supplied), 1uL RNasin (Promega cat. no. N251B) and the volume was increased to 15 μL with DIW.

Size-exclusion Chromatography:

Article Title: A New Method for Stranded Whole Transcriptome RNA-seq
Article Snippet: Starting with 2 μg total RNA derived from cells, combine with 1.5 μL DNase (1.5 U, Promega Corporation, 2800 Woods Hollow Rd, Madison, WI 53711, cat. no. M198A), 3 μL 5X MMLV reverse transcriptase buffer (Promega supplied), 1uL RNasin (Promega cat. no. N251B) and the volume was increased to 15 μL with DIW. .. Prior to qRT-PCR, the cDNA was diluted with 160 μL DIW, and the sample was stored at −20°C. qRT-PCR was carried out as described in sec. 2.12 except that 5 μL of diluted cDNA was used instead of diluted RNA-seq PCR1.

Random Hexamer Labeling:

Article Title: A New Method for Stranded Whole Transcriptome RNA-seq
Article Snippet: Starting with 2 μg total RNA derived from cells, combine with 1.5 μL DNase (1.5 U, Promega Corporation, 2800 Woods Hollow Rd, Madison, WI 53711, cat. no. M198A), 3 μL 5X MMLV reverse transcriptase buffer (Promega supplied), 1uL RNasin (Promega cat. no. N251B) and the volume was increased to 15 μL with DIW. .. The DNase reaction was incubated for 60 min at 37°C and then inactivated with DNase at 80°C for 5 min. cDNA was made by adding 2 μL/1μg random hexamer (Qiagen Sciences, Valencia, CA 91355, cat. no. 79236) and denatured at 70°C for 2 min. Then, the DNase-treated RNA was combined with 5 μL 5X MMLV reverse transcriptase buffer (Promega supplied), 2 μL 10 mM dNTP mix (Promega cat. no. U1511), 1 μL RNasin (Promega cat. no. N251B), 1.6 μL MMLV Reverse Transcriptase (Promega cat. no. M1708), 13.4 μL DIW and incubated at 42°C for 60 min, then the cDNA was denatured at 95°C for 5 min.

Purification:

Article Title: A New Method for Stranded Whole Transcriptome RNA-seq
Article Snippet: Starting with 2 μg total RNA derived from cells, combine with 1.5 μL DNase (1.5 U, Promega Corporation, 2800 Woods Hollow Rd, Madison, WI 53711, cat. no. M198A), 3 μL 5X MMLV reverse transcriptase buffer (Promega supplied), 1uL RNasin (Promega cat. no. N251B) and the volume was increased to 15 μL with DIW. .. All qRT-PCR primers (standard salt free purification) were obtained from Fisher Scientific, Pittsburgh, PA 15275.

Derivative Assay:

Article Title: A New Method for Stranded Whole Transcriptome RNA-seq
Article Snippet: .. Starting with 2 μg total RNA derived from cells, combine with 1.5 μL DNase (1.5 U, Promega Corporation, 2800 Woods Hollow Rd, Madison, WI 53711, cat. no. M198A), 3 μL 5X MMLV reverse transcriptase buffer (Promega supplied), 1uL RNasin (Promega cat. no. N251B) and the volume was increased to 15 μL with DIW. .. The DNase reaction was incubated for 60 min at 37°C and then inactivated with DNase at 80°C for 5 min. cDNA was made by adding 2 μL/1μg random hexamer (Qiagen Sciences, Valencia, CA 91355, cat. no. 79236) and denatured at 70°C for 2 min. Then, the DNase-treated RNA was combined with 5 μL 5X MMLV reverse transcriptase buffer (Promega supplied), 2 μL 10 mM dNTP mix (Promega cat. no. U1511), 1 μL RNasin (Promega cat. no. N251B), 1.6 μL MMLV Reverse Transcriptase (Promega cat. no. M1708), 13.4 μL DIW and incubated at 42°C for 60 min, then the cDNA was denatured at 95°C for 5 min.

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    Promega 1ul rnasin
    1ul Rnasin, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1ul rnasin/product/Promega
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    1ul rnasin - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

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