1m mgcl solution  (Millipore)

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  • 88

    Structured Review

    Millipore 1m mgcl solution
    1m Mgcl Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1m mgcl solution/product/Millipore
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    1m mgcl solution - by Bioz Stars, 2020-07
    88/100 stars


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    Activation Assay:

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    Article Snippet: .. On the day of the experiment, pack the required beads in a glass Econo-Column, wash and equilibrate in buffer E. 120 mL HiLoad 16/60 Superdex 200 prep grade column (Amersham) Mono Q HR 10/10 anion exchange column (Amersham) Luria Broth (LB) medium (Sigma) containing 50 μg/mL ampicillin sodium (Sigma) Luria Broth (LB) medium containing 30 μ g/mL kanamycin (Sigma) Terrific Broth (TB) medium (Sigma) containing 50 μg/mL ampicillin sodium (Sigma) 1 M Isopropyl β-D-1-thiogalactopyranoside (IPTG) solution in sterile water (Sigma) 1M MgCl solution (Sigma) Lysozyme lyophilized enzyme (MP Biomedicals) 100 % Glycerol 10% ( v / v ) Triton in water (Sigma) 50 mM ATP (Roche) in 25 mM HEPES buffer (pH 7.5) NaCl (Biological grade MP Biomedical) Imidazole CaCl2 Bradford reagent (Biorad) GST-MEKK1c (C terminal 320 amino acids corresponding to the catalytic domain) is necessary for the activation of GST-MKK4, GST-MKK7-His6 . .. The protocol for GST-MEKK1c vector construction and protein purification has been previously described ( ; ).