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Millipore 1m cacl2
1m Cacl2, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
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1m cacl2 - by Bioz Stars, 2020-02
99/100 stars

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Centrifugation:

Article Title: An Epitope-Substituted DNA Vaccine Improves Safety and Immunogenicity against Dengue Virus Type 2
Article Snippet: Then, 50 μg of plasmid DNA was added, followed by the addition of 1M CaCl2 (Sigma-Aldrich, St. Louis, MO); the solution was mixed and precipitated for 10 mins at RT. .. After collection by centrifugation, the gold-DNA complex was washed with absolute ethanol and resuspended in 0.1 mg/ml of polyvinylpyrrolidone (PVP) (360 kDa; Sigma Chemicals, Inc.) solution.

Article Title: Oct4 and Hnf4α-induced hepatic stem cells ameliorate chronic liver injury in liver fibrosis model
Article Snippet: Livers were perfused in situ through the inferior cava vein, with Hanks’ balanced salt solution (Sigma) containing 50mM EGTA, 1M Glucose and 1% penicillin/streptomycin, followed by Hanks’ balanced salt solution containing 1M Cacl2, 0.025% collagenase (Sigma) until loss of its firm texture. .. The pellet was resuspended with Williams’ medium E (Thermo Fisher Scientific) supplemented with 1% penicillin/streptomycin and carefully overlay 13.5% Percoll solution then centrifuged at 1500 rpm for 8 min. After centrifugation, the pellet was washed three times and resuspended in Williams’ medium E supplemented with 1% penicillin/streptomycin and L-glutamine.

Affinity Chromatography:

Article Title: Development and analytical validation of an enzyme linked immunosorbent assay for the measurement of canine gastric lipase immunoreactivity in serum
Article Snippet: Paragraph title: Affinity chromatography of antiserum ... Ten milliliters of anti-serum were added to 10 mL 1M CaCl2 (Sigma Chemical) and 400 μL 10% dextran sulfate (Sigma Chemical), mixed for 20 min using a rocking plate (VWR Scientific) at 4°C, and centrifuged at 10 000 × g and 4°C for 30 min.

In Situ:

Article Title: Oct4 and Hnf4α-induced hepatic stem cells ameliorate chronic liver injury in liver fibrosis model
Article Snippet: .. Livers were perfused in situ through the inferior cava vein, with Hanks’ balanced salt solution (Sigma) containing 50mM EGTA, 1M Glucose and 1% penicillin/streptomycin, followed by Hanks’ balanced salt solution containing 1M Cacl2, 0.025% collagenase (Sigma) until loss of its firm texture. ..

Mutagenesis:

Article Title: An Epitope-Substituted DNA Vaccine Improves Safety and Immunogenicity against Dengue Virus Type 2
Article Snippet: Preparation of plasmids for immunization Plasmids expressing WT E protein of DENV2 or a mutant E protein in which the N8 residue was substituted with R (N8R) were used for immunization. .. Then, 50 μg of plasmid DNA was added, followed by the addition of 1M CaCl2 (Sigma-Aldrich, St. Louis, MO); the solution was mixed and precipitated for 10 mins at RT.

Isolation:

Article Title: Oct4 and Hnf4α-induced hepatic stem cells ameliorate chronic liver injury in liver fibrosis model
Article Snippet: Paragraph title: Isolation of mouse primary hepatocytes ... Livers were perfused in situ through the inferior cava vein, with Hanks’ balanced salt solution (Sigma) containing 50mM EGTA, 1M Glucose and 1% penicillin/streptomycin, followed by Hanks’ balanced salt solution containing 1M Cacl2, 0.025% collagenase (Sigma) until loss of its firm texture.

Cell Culture:

Article Title: Osteoclast-Like Cells in Aneurysmal Disease Exhibit an Enhanced Proteolytic Phenotype
Article Snippet: Paragraph title: 4.1. Cell Culture and Treatments ... The CaPO4 crystals were prepared by a 1:10 dilution of 1M CaCl2 (Sigma-Aldrich, St. Louis, MO, USA) to phosphate-buffered saline (PBS) (Sigma-Aldrich).

Purification:

Article Title: Development and analytical validation of an enzyme linked immunosorbent assay for the measurement of canine gastric lipase immunoreactivity in serum
Article Snippet: Antisera were prepared for purification by lipoprotein precipitation. .. Ten milliliters of anti-serum were added to 10 mL 1M CaCl2 (Sigma Chemical) and 400 μL 10% dextran sulfate (Sigma Chemical), mixed for 20 min using a rocking plate (VWR Scientific) at 4°C, and centrifuged at 10 000 × g and 4°C for 30 min.

Concentration Assay:

Article Title: An orphaned Mce-associated membrane protein of Mycobacterium tuberculosis is a virulence factor that stabilizes Mce transporters
Article Snippet: M9 minimal media was prepared as follows: 1L dH2 O, 12.8g Na2 HPO4 , 3g KH2 PO4 , 0.5g NaCl, 1g NH4 Cl, 25 μL 1M CaCl2, 500μL 1M MgSO4 , and 2.5 mL 10% Tyloxapol (Ty, Sigma), and supplemented with 0.2% glucose and 0.5% glycerol or 0.5mM cholesterol from stock solution. .. Plates were incubated shaking at 37°C overnight, after which resazurin (Sigma) was added to a final concentration 0.0125 mg mL−1 .

Incubation:

Article Title: An orphaned Mce-associated membrane protein of Mycobacterium tuberculosis is a virulence factor that stabilizes Mce transporters
Article Snippet: M9 minimal media was prepared as follows: 1L dH2 O, 12.8g Na2 HPO4 , 3g KH2 PO4 , 0.5g NaCl, 1g NH4 Cl, 25 μL 1M CaCl2, 500μL 1M MgSO4 , and 2.5 mL 10% Tyloxapol (Ty, Sigma), and supplemented with 0.2% glucose and 0.5% glycerol or 0.5mM cholesterol from stock solution. .. Plates were incubated shaking at 37°C overnight, after which resazurin (Sigma) was added to a final concentration 0.0125 mg mL−1 .

Affinity Column:

Article Title: Development and analytical validation of an enzyme linked immunosorbent assay for the measurement of canine gastric lipase immunoreactivity in serum
Article Snippet: An affinity chromatography column (Hi Trap; Amersham Pharmacia Biotech) for the purification of monospecific polyclonal antibodies against cGL was prepared following the manufacturer’s instructions. .. Ten milliliters of anti-serum were added to 10 mL 1M CaCl2 (Sigma Chemical) and 400 μL 10% dextran sulfate (Sigma Chemical), mixed for 20 min using a rocking plate (VWR Scientific) at 4°C, and centrifuged at 10 000 × g and 4°C for 30 min.

Expressing:

Article Title: An Epitope-Substituted DNA Vaccine Improves Safety and Immunogenicity against Dengue Virus Type 2
Article Snippet: Preparation of plasmids for immunization Plasmids expressing WT E protein of DENV2 or a mutant E protein in which the N8 residue was substituted with R (N8R) were used for immunization. .. Then, 50 μg of plasmid DNA was added, followed by the addition of 1M CaCl2 (Sigma-Aldrich, St. Louis, MO); the solution was mixed and precipitated for 10 mins at RT.

Modification:

Article Title: Osteoclast-Like Cells in Aneurysmal Disease Exhibit an Enhanced Proteolytic Phenotype
Article Snippet: For macrophage stimulation, macrophages were maintained in modified Eagle’s medium-alpha (MEM-α) (Mediatech) supplemented with 10% charcoal-stripped FBS (Mediatech), penicillin, and streptomycin, and stimulated with or without 100 ng/mL TNFα (Peprotech, Rocky Hill, NJ, USA) plus 2% CaPO4 (v /v ). .. The CaPO4 crystals were prepared by a 1:10 dilution of 1M CaCl2 (Sigma-Aldrich, St. Louis, MO, USA) to phosphate-buffered saline (PBS) (Sigma-Aldrich).

Injection:

Article Title: An Epitope-Substituted DNA Vaccine Improves Safety and Immunogenicity against Dengue Virus Type 2
Article Snippet: Then, 50 μg of plasmid DNA was added, followed by the addition of 1M CaCl2 (Sigma-Aldrich, St. Louis, MO); the solution was mixed and precipitated for 10 mins at RT. .. The slurry was injected into a TefzelR tube (McMaster-Carr, Chicago, IL), and then coated.

Mouse Assay:

Article Title: Oct4 and Hnf4α-induced hepatic stem cells ameliorate chronic liver injury in liver fibrosis model
Article Snippet: In brief, mice were anesthetized with isoflurane. .. Livers were perfused in situ through the inferior cava vein, with Hanks’ balanced salt solution (Sigma) containing 50mM EGTA, 1M Glucose and 1% penicillin/streptomycin, followed by Hanks’ balanced salt solution containing 1M Cacl2, 0.025% collagenase (Sigma) until loss of its firm texture.

Plasmid Preparation:

Article Title: An Epitope-Substituted DNA Vaccine Improves Safety and Immunogenicity against Dengue Virus Type 2
Article Snippet: .. Then, 50 μg of plasmid DNA was added, followed by the addition of 1M CaCl2 (Sigma-Aldrich, St. Louis, MO); the solution was mixed and precipitated for 10 mins at RT. .. After collection by centrifugation, the gold-DNA complex was washed with absolute ethanol and resuspended in 0.1 mg/ml of polyvinylpyrrolidone (PVP) (360 kDa; Sigma Chemicals, Inc.) solution.