proteinase k no 19131  (Qiagen)


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    QIAGEN Proteinase K
    Description:
    For protease digestion during DNA and RNA preparation Kit contents Qiagen Proteinase K 2mL 600mAU mL Activity Ready to use Solution For Protease Digestion During DNA and RNA Preparation Offer Broad Substrate Specificity with High Activity for a Wide Range of Reaction Conditions Used in Most DNA and RNA Isolation
    Catalog Number:
    19131
    Price:
    96.3
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    QIAGEN Proteinase K
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    Structured Review

    Qiagen proteinase k no 19131
    QIAGEN Proteinase K
    For protease digestion during DNA and RNA preparation Kit contents Qiagen Proteinase K 2mL 600mAU mL Activity Ready to use Solution For Protease Digestion During DNA and RNA Preparation Offer Broad Substrate Specificity with High Activity for a Wide Range of Reaction Conditions Used in Most DNA and RNA Isolation
    https://www.bioz.com/result/proteinase k no 19131/product/Qiagen
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    proteinase k no 19131 - by Bioz Stars, 2020-01
    90/100 stars

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    Amplification:

    Article Title: Species delimitation in northern European water scavenger beetles of the genus Hydrobius ( Coleoptera, Hydrophilidae)
    Article Snippet: Paragraph title: DNA extraction, amplification and sequencing ... Beetles were placed in 100 µL Lysis Buffer (Mole Genetics, Lysaker, Norway) and 4 µL QIAGEN® Proteinase-K (QIAGEN, Venlo, Netherlands) and incubated overnight at 56 °C for 7-12 hours.

    Blocking Assay:

    Article Title: Evaluation of the relationship between plaque formation leading to symptomatic carotid artery stenosis and cytomegalovirus by investigating the virus DNA
    Article Snippet: Operations were performed with continuous neurological evaluation during carotid arterial clamping under cervical regional block. .. Subsequently, 400 μl of tissue lysis solution and 40 μl of proteinase K were added to 2 ml sterile Eppendorf tubes to perform 56°C overnight tissue disruption (Buffer ATL (Cat no: 939011), Proteinase K (Cat no: 19133; Qiagen)).

    Real-time Polymerase Chain Reaction:

    Article Title: Dose-dependent alcohol-induced alterations in chromatin structure persist beyond the window of exposure and correlate with fetal alcohol syndrome birth defects
    Article Snippet: We utilized glucosylation of genomic DNA followed by methylation-sensitive qPCR (glucMS-qPCR) to quantify levels of 5-methyl-cytosine and 5-hydroxy-methyl-cytosine. .. Reactions were inactivated by treatment with proteinase K (Cat# 19133; QIAGEN) at 40 °C for 30 min.

    Article Title: Epigenomics: maternal high-fat diet exposure in utero disrupts peripheral circadian gene expression in nonhuman primates
    Article Snippet: Beads were washed, resuspended in buffer containing proteinase K (19131; Qiagen), and incubated overnight with shaking at 37°C. .. Enrichment of promoter occupancy by virtue of histone modifications was determined using qPCR with the following primers: Npas2 RORE (5′-GTTTTCACCGGCTCTGTCTTG-3′ and 5′-GAGGCAGAGGTACAGGAATAATCC-3′) and Npas2 intron (5′-GCCGTTTGCTTCTTGGAAA-3′ and 5′-AACCGATTCTACATTTACGCACAGT-3′).

    Incubation:

    Article Title: Development of a sensitive RT-PCR method for amplifying and sequencing near full-length HCV genotype 1 RNA from patient samples
    Article Snippet: Plasma (220 ~ 660 μL, the volume was adjusted based on viral load, i.e., 220 μL for HCV RNA greater than or equal to 50,000 IU/mL, while 660 μL for HCV RNA less than 50,000 IU/mL) was mixed with QIAGEN Protease K (40 ~ 120 μL) and QIAamp AL buffer (240 ~ 720 μL) supplemented with 20 ~ 60 μg of carrier RNA (tRNA) per column. .. The mixture was incubated for 15 min at 56°C, and absolute ethanol (293 ~ 875 μL) was added to each well and mixed well.

    Article Title: Dose-dependent alcohol-induced alterations in chromatin structure persist beyond the window of exposure and correlate with fetal alcohol syndrome birth defects
    Article Snippet: Reactions were inactivated by treatment with proteinase K (Cat# 19133; QIAGEN) at 40 °C for 30 min. .. The proteinase K was inactivated by incubation at 95 °C for 10 min.

    Article Title: Real-Time Polymerase Chain Reaction for Detecting Bacterial DNA Directly from Blood of Neonates Being Evaluated for Sepsis
    Article Snippet: .. A modified protocol using the QIAamp DNA Mini kit (Qiagen Inc., Valencia, CA) was performed as follows: 20 μl of QIAGEN Proteinase K (20 mg/ml) was added for every 200 μl of whole blood processed along with an equal volume (200 μl) of buffer AL, and the sample was incubated for 30 minutes at 57°C. .. After incubation, an equal volume (200 μl) of 100% ethanol was added, and the resulting lysate was loaded onto the QIAamp DNA Mini kit column (Qiagen).

    Article Title: Species delimitation in northern European water scavenger beetles of the genus Hydrobius ( Coleoptera, Hydrophilidae)
    Article Snippet: .. Beetles were placed in 100 µL Lysis Buffer (Mole Genetics, Lysaker, Norway) and 4 µL QIAGEN® Proteinase-K (QIAGEN, Venlo, Netherlands) and incubated overnight at 56 °C for 7-12 hours. .. The lysate was transferred to sample tubes after lysis and MoleStripsTM DNA Tissue (Mole Genetics) was used to extract DNA using a GeneMole® robot (Mole Genetics).

    Article Title: Epigenomics: maternal high-fat diet exposure in utero disrupts peripheral circadian gene expression in nonhuman primates
    Article Snippet: .. Beads were washed, resuspended in buffer containing proteinase K (19131; Qiagen), and incubated overnight with shaking at 37°C. .. Concomitantly, proteinase K was added to the input samples and incubated overnight at 37°C.

    Article Title: Technical Reproducibility of Single-Nucleotide and Size-Based DNA Biomarker Assessment Using DNA Extracted from Formalin-Fixed, Paraffin-Embedded Tissues
    Article Snippet: .. Briefly, 10 to 15 mg frozen tissue was homogenized and incubated in buffer G2 containing RNase A and Qiagen Protease K, then incubated at 50°C for 4 hours. .. DNA was resuspended in 100 to 200 μL of nuclease-free water (Life Technologies, Carlsbad, CA) and dissolved overnight on a shaker.

    Article Title: A cross-sectional investigation into the association between Porphyromonas gingivalis and autoantibodies to citrullinated proteins in a German population
    Article Snippet: Then, 180 µl of buffer ATL and 20 µl of QIAGEN proteinase K were consecutively added to the pelleted sample. .. The mixture was vortexed and incubated at 56°C for 30 min, and purified using ethanol-containing buffers.

    Diffusion-based Assay:

    Article Title: Species delimitation in northern European water scavenger beetles of the genus Hydrobius ( Coleoptera, Hydrophilidae)
    Article Snippet: The second or third abdominal ventrite of the specimens was punctured with sharp sterile forceps to facilitate lysis and diffusion of DNA out of the specimens. .. Beetles were placed in 100 µL Lysis Buffer (Mole Genetics, Lysaker, Norway) and 4 µL QIAGEN® Proteinase-K (QIAGEN, Venlo, Netherlands) and incubated overnight at 56 °C for 7-12 hours.

    Mass Spectrometry:

    Article Title: Emergence of a Streptococcus dysgalactiae subspecies equisimilis stG62647-lineage associated with severe clinical manifestations
    Article Snippet: Serogroup specificity had previously been determined using a rapid agglutination test (Oxoid Streptococcal Grouping Kit, Hampshire, UK), and species identity had been confirmed by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-ToF MS), using Microflex™ with the MALDI Biotyper database (Bruker Daltonik, Bremen, Germany). .. Bacterial suspensions were pre-treated with Mutanolysin (M9901, Sigma-Aldrich, Dorset, UK), Lysozyme (L6876, Sigma-Aldrich) and Proteinase K (19133, Qiagen, Hilden, Germany), and genomic DNA was extracted using MagNA Pure 96 (Roche Life science).

    Modification:

    Article Title: Real-Time Polymerase Chain Reaction for Detecting Bacterial DNA Directly from Blood of Neonates Being Evaluated for Sepsis
    Article Snippet: .. A modified protocol using the QIAamp DNA Mini kit (Qiagen Inc., Valencia, CA) was performed as follows: 20 μl of QIAGEN Proteinase K (20 mg/ml) was added for every 200 μl of whole blood processed along with an equal volume (200 μl) of buffer AL, and the sample was incubated for 30 minutes at 57°C. .. After incubation, an equal volume (200 μl) of 100% ethanol was added, and the resulting lysate was loaded onto the QIAamp DNA Mini kit column (Qiagen).

    Article Title: Development of a TaqMan Array card to target 21 purulent meningitis-related pathogens
    Article Snippet: The modified protocol of QIAamp cador pathogen Mini Kit was used. .. The lysis tube was centrifuged for 5 min at 14,000×g, and then 200 μL of the supernatant was transferred to a 1.5 mL microcentrifuge tube containing 20 μL QIAGEN Protease K. A total of 400 μL lysis buffer was added into the pathogen lysis tube to re-suspend the pellet.

    Agglutination:

    Article Title: Emergence of a Streptococcus dysgalactiae subspecies equisimilis stG62647-lineage associated with severe clinical manifestations
    Article Snippet: Serogroup specificity had previously been determined using a rapid agglutination test (Oxoid Streptococcal Grouping Kit, Hampshire, UK), and species identity had been confirmed by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-ToF MS), using Microflex™ with the MALDI Biotyper database (Bruker Daltonik, Bremen, Germany). .. Bacterial suspensions were pre-treated with Mutanolysin (M9901, Sigma-Aldrich, Dorset, UK), Lysozyme (L6876, Sigma-Aldrich) and Proteinase K (19133, Qiagen, Hilden, Germany), and genomic DNA was extracted using MagNA Pure 96 (Roche Life science).

    Cell Culture:

    Article Title: Technical Reproducibility of Single-Nucleotide and Size-Based DNA Biomarker Assessment Using DNA Extracted from Formalin-Fixed, Paraffin-Embedded Tissues
    Article Snippet: Genomic DNA was extracted from frozen tissues and cell lines using a blood and cell culture DNA extraction kit, according to the manufacturer's instructions (Qiagen, Valencia, CA). .. Briefly, 10 to 15 mg frozen tissue was homogenized and incubated in buffer G2 containing RNase A and Qiagen Protease K, then incubated at 50°C for 4 hours.

    other:

    Article Title: Optimization to detect TP53 mutations in circulating cell-free tumor DNA from patients with serous epithelial ovarian cancer
    Article Snippet: Qiagen proteinase K® (Qiagen; 500 µL) was placed in a 50-mL tube, followed by the addition of 4 mL ACL buffer and 5 mL plasma; the mixture was mixed for 30 seconds and then maintained at 60°C for 30 minutes, followed by the addition of 3.6 mL of ACB buffer.

    Imaging:

    Article Title: Evaluation of the relationship between plaque formation leading to symptomatic carotid artery stenosis and cytomegalovirus by investigating the virus DNA
    Article Snippet: Preoperative carotid artery stenosis rates were provided from preoperative radiological imaging results (such as carotid duplex ultrasonography, computed tomography angiography, magnetic resonance angiography, and subtraction angiography). .. Subsequently, 400 μl of tissue lysis solution and 40 μl of proteinase K were added to 2 ml sterile Eppendorf tubes to perform 56°C overnight tissue disruption (Buffer ATL (Cat no: 939011), Proteinase K (Cat no: 19133; Qiagen)).

    Polymerase Chain Reaction:

    Article Title: Real-Time Polymerase Chain Reaction for Detecting Bacterial DNA Directly from Blood of Neonates Being Evaluated for Sepsis
    Article Snippet: Paragraph title: Whole-Blood Sample Processing for PCR ... A modified protocol using the QIAamp DNA Mini kit (Qiagen Inc., Valencia, CA) was performed as follows: 20 μl of QIAGEN Proteinase K (20 mg/ml) was added for every 200 μl of whole blood processed along with an equal volume (200 μl) of buffer AL, and the sample was incubated for 30 minutes at 57°C.

    Article Title: A real-time, quantitative PCR method using hydrolysis probes for the monitoring of Plasmodium falciparum load in experimentally infected human volunteers
    Article Snippet: Extraction of nucleic acid was achieved by adding 40 μl of Qiagen Protease K and incubating each sample at 56°C for 10 minutes. .. Nucleic acid extracts were eluted in 100 μl of elution buffer and stored at -80°C until PCR reactions were performed.

    Article Title: Evaluation of the relationship between plaque formation leading to symptomatic carotid artery stenosis and cytomegalovirus by investigating the virus DNA
    Article Snippet: Subsequently, 400 μl of tissue lysis solution and 40 μl of proteinase K were added to 2 ml sterile Eppendorf tubes to perform 56°C overnight tissue disruption (Buffer ATL (Cat no: 939011), Proteinase K (Cat no: 19133; Qiagen)). .. Quantitation of CMV samples in the green channel was performed by PCR analysis.

    Computed Tomography:

    Article Title: Evaluation of the relationship between plaque formation leading to symptomatic carotid artery stenosis and cytomegalovirus by investigating the virus DNA
    Article Snippet: Preoperative carotid artery stenosis rates were provided from preoperative radiological imaging results (such as carotid duplex ultrasonography, computed tomography angiography, magnetic resonance angiography, and subtraction angiography). .. Subsequently, 400 μl of tissue lysis solution and 40 μl of proteinase K were added to 2 ml sterile Eppendorf tubes to perform 56°C overnight tissue disruption (Buffer ATL (Cat no: 939011), Proteinase K (Cat no: 19133; Qiagen)).

    DNA Extraction:

    Article Title: A real-time, quantitative PCR method using hydrolysis probes for the monitoring of Plasmodium falciparum load in experimentally infected human volunteers
    Article Snippet: Before DNA extraction, 104 copies of equine herpes virus (EHV) DNA (Cp = 30) were added to each of the filtered and unfiltered sample dilutions to monitor the efficiency and reproducibility of the extraction process [ ]. .. Extraction of nucleic acid was achieved by adding 40 μl of Qiagen Protease K and incubating each sample at 56°C for 10 minutes.

    Article Title: Species delimitation in northern European water scavenger beetles of the genus Hydrobius ( Coleoptera, Hydrophilidae)
    Article Snippet: Paragraph title: DNA extraction, amplification and sequencing ... Beetles were placed in 100 µL Lysis Buffer (Mole Genetics, Lysaker, Norway) and 4 µL QIAGEN® Proteinase-K (QIAGEN, Venlo, Netherlands) and incubated overnight at 56 °C for 7-12 hours.

    Article Title: Technical Reproducibility of Single-Nucleotide and Size-Based DNA Biomarker Assessment Using DNA Extracted from Formalin-Fixed, Paraffin-Embedded Tissues
    Article Snippet: Genomic DNA was extracted from frozen tissues and cell lines using a blood and cell culture DNA extraction kit, according to the manufacturer's instructions (Qiagen, Valencia, CA). .. Briefly, 10 to 15 mg frozen tissue was homogenized and incubated in buffer G2 containing RNase A and Qiagen Protease K, then incubated at 50°C for 4 hours.

    Article Title: Emergence of a Streptococcus dysgalactiae subspecies equisimilis stG62647-lineage associated with severe clinical manifestations
    Article Snippet: Paragraph title: Bacterial isolates, DNA extraction and sequencing ... Bacterial suspensions were pre-treated with Mutanolysin (M9901, Sigma-Aldrich, Dorset, UK), Lysozyme (L6876, Sigma-Aldrich) and Proteinase K (19133, Qiagen, Hilden, Germany), and genomic DNA was extracted using MagNA Pure 96 (Roche Life science).

    Article Title: Genotypes and serum concentrations of human alpha-1-antitrypsin "P" protein variants in a clinical population
    Article Snippet: Paragraph title: DNA extraction ... The manufacturer's spin column protocol was followed with the following modifications: Qiagen Proteinase K (20 µl) was utilised in the lysis step, and the final elution was performed using 150 µl of buffer AE.

    Methylation:

    Article Title: Dose-dependent alcohol-induced alterations in chromatin structure persist beyond the window of exposure and correlate with fetal alcohol syndrome birth defects
    Article Snippet: We utilized glucosylation of genomic DNA followed by methylation-sensitive qPCR (glucMS-qPCR) to quantify levels of 5-methyl-cytosine and 5-hydroxy-methyl-cytosine. .. Reactions were inactivated by treatment with proteinase K (Cat# 19133; QIAGEN) at 40 °C for 30 min.

    Isolation:

    Article Title: Dose-dependent alcohol-induced alterations in chromatin structure persist beyond the window of exposure and correlate with fetal alcohol syndrome birth defects
    Article Snippet: Analysis of DNA methylation and DNA hydroxymethylation Genomic DNA was isolated from treated neurospheres using the DNeasy Blood and Tissue kit (Cat# 69506; QIAGEN) according to the recommended protocol. .. Reactions were inactivated by treatment with proteinase K (Cat# 19133; QIAGEN) at 40 °C for 30 min.

    Article Title: Evaluation of the relationship between plaque formation leading to symptomatic carotid artery stenosis and cytomegalovirus by investigating the virus DNA
    Article Snippet: Subsequently, 400 μl of tissue lysis solution and 40 μl of proteinase K were added to 2 ml sterile Eppendorf tubes to perform 56°C overnight tissue disruption (Buffer ATL (Cat no: 939011), Proteinase K (Cat no: 19133; Qiagen)). .. In the yellow channel, the internal control used during the isolation phase was assessed, which allows us to check the isolation and PCR stage correctly.

    Article Title: A cross-sectional investigation into the association between Porphyromonas gingivalis and autoantibodies to citrullinated proteins in a German population
    Article Snippet: Paragraph title: Isolation of genomic DNA from saliva ... Then, 180 µl of buffer ATL and 20 µl of QIAGEN proteinase K were consecutively added to the pelleted sample.

    Purification:

    Article Title: Epigenomics: maternal high-fat diet exposure in utero disrupts peripheral circadian gene expression in nonhuman primates
    Article Snippet: Beads were washed, resuspended in buffer containing proteinase K (19131; Qiagen), and incubated overnight with shaking at 37°C. .. Cross-links were reversed by incubation at 65°C for 5 h. DNA was purified using phenol-chloroform extraction followed by ethanol precipitation.

    Article Title: RNA-Binding Motif Protein 24 (RBM24) Is Involved in Pregenomic RNA Packaging by Mediating Interaction between Hepatitis B Virus Polymerase and the Epsilon Element
    Article Snippet: Paragraph title: Purification of HBV virions. ... The resuspended virions were then treated with 0.5 μg/μl proteinase K (catalog number 19133; Qiagen) to remove nonencapsidated proteins.

    Article Title: A cross-sectional investigation into the association between Porphyromonas gingivalis and autoantibodies to citrullinated proteins in a German population
    Article Snippet: Then, 180 µl of buffer ATL and 20 µl of QIAGEN proteinase K were consecutively added to the pelleted sample. .. The mixture was vortexed and incubated at 56°C for 30 min, and purified using ethanol-containing buffers.

    Sequencing:

    Article Title: Species delimitation in northern European water scavenger beetles of the genus Hydrobius ( Coleoptera, Hydrophilidae)
    Article Snippet: Paragraph title: DNA extraction, amplification and sequencing ... Beetles were placed in 100 µL Lysis Buffer (Mole Genetics, Lysaker, Norway) and 4 µL QIAGEN® Proteinase-K (QIAGEN, Venlo, Netherlands) and incubated overnight at 56 °C for 7-12 hours.

    Article Title: Emergence of a Streptococcus dysgalactiae subspecies equisimilis stG62647-lineage associated with severe clinical manifestations
    Article Snippet: Paragraph title: Bacterial isolates, DNA extraction and sequencing ... Bacterial suspensions were pre-treated with Mutanolysin (M9901, Sigma-Aldrich, Dorset, UK), Lysozyme (L6876, Sigma-Aldrich) and Proteinase K (19133, Qiagen, Hilden, Germany), and genomic DNA was extracted using MagNA Pure 96 (Roche Life science).

    Chromatin Immunoprecipitation:

    Article Title: Epigenomics: maternal high-fat diet exposure in utero disrupts peripheral circadian gene expression in nonhuman primates
    Article Snippet: Paragraph title: ChIP and analysis ... Beads were washed, resuspended in buffer containing proteinase K (19131; Qiagen), and incubated overnight with shaking at 37°C.

    RNA Extraction:

    Article Title: Development of a sensitive RT-PCR method for amplifying and sequencing near full-length HCV genotype 1 RNA from patient samples
    Article Snippet: Paragraph title: HCV RNA extraction ... Plasma (220 ~ 660 μL, the volume was adjusted based on viral load, i.e., 220 μL for HCV RNA greater than or equal to 50,000 IU/mL, while 660 μL for HCV RNA less than 50,000 IU/mL) was mixed with QIAGEN Protease K (40 ~ 120 μL) and QIAamp AL buffer (240 ~ 720 μL) supplemented with 20 ~ 60 μg of carrier RNA (tRNA) per column.

    Selection:

    Article Title: Species delimitation in northern European water scavenger beetles of the genus Hydrobius ( Coleoptera, Hydrophilidae)
    Article Snippet: Beetles were placed in 100 µL Lysis Buffer (Mole Genetics, Lysaker, Norway) and 4 µL QIAGEN® Proteinase-K (QIAGEN, Venlo, Netherlands) and incubated overnight at 56 °C for 7-12 hours. .. A selection of the specimens (n = 5) went through the DNA extraction process twice to be used as controls.

    Sample Prep:

    Article Title: A real-time, quantitative PCR method using hydrolysis probes for the monitoring of Plasmodium falciparum load in experimentally infected human volunteers
    Article Snippet: Paragraph title: Sample preparation and nucleic acid extraction ... Extraction of nucleic acid was achieved by adding 40 μl of Qiagen Protease K and incubating each sample at 56°C for 10 minutes.

    Ethanol Precipitation:

    Article Title: Epigenomics: maternal high-fat diet exposure in utero disrupts peripheral circadian gene expression in nonhuman primates
    Article Snippet: Beads were washed, resuspended in buffer containing proteinase K (19131; Qiagen), and incubated overnight with shaking at 37°C. .. Cross-links were reversed by incubation at 65°C for 5 h. DNA was purified using phenol-chloroform extraction followed by ethanol precipitation.

    Quantitation Assay:

    Article Title: Evaluation of the relationship between plaque formation leading to symptomatic carotid artery stenosis and cytomegalovirus by investigating the virus DNA
    Article Snippet: Subsequently, 400 μl of tissue lysis solution and 40 μl of proteinase K were added to 2 ml sterile Eppendorf tubes to perform 56°C overnight tissue disruption (Buffer ATL (Cat no: 939011), Proteinase K (Cat no: 19133; Qiagen)). .. Quantitation of CMV samples in the green channel was performed by PCR analysis.

    Spectrophotometry:

    Article Title: A cross-sectional investigation into the association between Porphyromonas gingivalis and autoantibodies to citrullinated proteins in a German population
    Article Snippet: Then, 180 µl of buffer ATL and 20 µl of QIAGEN proteinase K were consecutively added to the pelleted sample. .. The gDNA was eluted in nuclease-free water, followed by quantification and quality assessment (A260) with a NanoVue spectrophotometer (GE Healthcare Bio-Sciences, Uppsala, Sweden) and stored at −20°C until analysis.

    DNA Methylation Assay:

    Article Title: Dose-dependent alcohol-induced alterations in chromatin structure persist beyond the window of exposure and correlate with fetal alcohol syndrome birth defects
    Article Snippet: Paragraph title: Analysis of DNA methylation and DNA hydroxymethylation ... Reactions were inactivated by treatment with proteinase K (Cat# 19133; QIAGEN) at 40 °C for 30 min.

    Lysis:

    Article Title: Species delimitation in northern European water scavenger beetles of the genus Hydrobius ( Coleoptera, Hydrophilidae)
    Article Snippet: .. Beetles were placed in 100 µL Lysis Buffer (Mole Genetics, Lysaker, Norway) and 4 µL QIAGEN® Proteinase-K (QIAGEN, Venlo, Netherlands) and incubated overnight at 56 °C for 7-12 hours. .. The lysate was transferred to sample tubes after lysis and MoleStripsTM DNA Tissue (Mole Genetics) was used to extract DNA using a GeneMole® robot (Mole Genetics).

    Article Title: Development of a TaqMan Array card to target 21 purulent meningitis-related pathogens
    Article Snippet: .. The lysis tube was centrifuged for 5 min at 14,000×g, and then 200 μL of the supernatant was transferred to a 1.5 mL microcentrifuge tube containing 20 μL QIAGEN Protease K. A total of 400 μL lysis buffer was added into the pathogen lysis tube to re-suspend the pellet. .. The pathogen lysis was then extracted as the manual.

    Article Title: Genotypes and serum concentrations of human alpha-1-antitrypsin "P" protein variants in a clinical population
    Article Snippet: .. The manufacturer's spin column protocol was followed with the following modifications: Qiagen Proteinase K (20 µl) was utilised in the lysis step, and the final elution was performed using 150 µl of buffer AE. .. The 394‐residue AAT protein product is produced by the SERPINA1 gene located at q31–32.3 on chromosome 14 (genebank accession # ).

    Article Title: Evaluation of the relationship between plaque formation leading to symptomatic carotid artery stenosis and cytomegalovirus by investigating the virus DNA
    Article Snippet: .. Subsequently, 400 μl of tissue lysis solution and 40 μl of proteinase K were added to 2 ml sterile Eppendorf tubes to perform 56°C overnight tissue disruption (Buffer ATL (Cat no: 939011), Proteinase K (Cat no: 19133; Qiagen)). .. Quantitation of CMV samples in the green channel was performed by PCR analysis.

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  • News
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  • Team
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  • Bioz Stars
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  • 90
    Qiagen qiagen protease k
    Qiagen Protease K, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiagen protease k/product/Qiagen
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    qiagen protease k - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

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