rnase (Qiagen)


Name:
RNase A
Description:
For RNase digestion during DNA preparation Kit contents Qiagen RNase A 17 500U 2 5mL 100mg mL Solution Endonuclease free Ready to use For RNase Digestion During DNA Preparation
Catalog Number:
19101
Price:
215
Category:
RNase A
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Structured Review
For RNase digestion during DNA preparation Kit contents Qiagen RNase A 17 500U 2 5mL 100mg mL Solution Endonuclease free Ready to use For RNase Digestion During DNA Preparation
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1) Product Images from "Activated human mesenchymal stem/stromal cells suppress metastatic features of MDA-MB-231 cells by secreting IFN-β"
Article Title: Activated human mesenchymal stem/stromal cells suppress metastatic features of MDA-MB-231 cells by secreting IFN-β
Journal: Cell Death & Disease
doi: 10.1038/cddis.2016.90

Figure Legend Snippet: IFN- β is derived from activated hMSCs, following coculture with MDA cells. ( a ) Quantitative RT-PCR for IFN- β from hMSCs after coculture with MDA (MB-231) cells. Values are mean±S.D. for triplicate of the assay. ( b ) Real-time RT-PCR for IFN- β from hMSCs treated with apoptotic MDA (MB-231) cells (Apot cells). Apoptotic MDA cells were treated with either RNase (R) or DNase (D). Values are mean±S.D. for triplicate of the assay. ( c ) Quantitative RT-PCR for IFN- β from hMSCs treated with TNF- α and either poly(I:C) or poly(dA:dT) (1 μ g/ml). Values are mean±S.D. for triplicate of the assay. ( d ) Quantitative RT-PCR for IFN- β from hMSCs after coculture with MDA (MB-231) cells. Before coculture, hMSCs were treated with siRNA for AIM2 (siAIM2 hMSCs) or IFIH1 (siIFIH1 hMSCs). Scrambled siRNA (siSCR hMSCs) were used as a control. Values are mean±S.D. for triplicate of the assay. ( e ) Quantitative RT-PCR assays for AIM2, IFIH1 and IFN- β in hMSCs, following coculture with Hcc38, MCF-7 and BT20 cells for 24 h. CC, hMSCs+cancer cells; CCT, hMSCs+cancer cells+TNF- α . Values are mean±S.D. for triplicate of the assay. ( f ) Western blot assay for TRAIL expression in BT20, Hcc38 and MCF-7 cells from control (C), TNF- α (T; 20 ng/ml) treatment or coculture with act hMSCs (MDA+hMSCs+TNF- α ; CCT)
Techniques Used: Derivative Assay, Multiple Displacement Amplification, Quantitative RT-PCR, Western Blot, Expressing, Activated Clotting Time Assay
2) Product Images from "Exosome can prevent RNase from degrading microRNA in feces"
Article Title: Exosome can prevent RNase from degrading microRNA in feces
Journal: Journal of Gastrointestinal Oncology
doi: 10.3978/j.issn.2078-6891.2011.015

Figure Legend Snippet: Degradation of naked RNA using RNase. (A) Electropherogram of total RNA treated with RNase. The total RNA is treated with 5 µg/mL of RNase for 0, 5, 10, 20, and 30 min at 4°C and 37°C. Two peaks, 18S and 28S ribosomal RNA (rRNA), are observed in total RNA without RNase treatment. (B) Relative quantification (RQ) of each miRNA treated with RNase at 4°C. The total RNA is treated with 5 µg/mL of RNase for 0, 5, 10, 20, and 30 min at 4°C. RQ of each miRNA is normalized by 18S rRNA (C) RQ of each miRNA treated with RNase at 37°C. The total RNA is treated with 5 µg/mL of RNase for 0, 5, 10, 20, and 30 min at 37°C. RQ of each miRNA is normalized by 18S rRNA.
Techniques Used:

Figure Legend Snippet: RQ of each miRNA in fecal samples treated with RNase. (A) RQ of each miRNA in cellular miRNA treated with RNase. Exfoliated colonocytes are treated with 5 µg/mL of RNase for 0, 30, 60, and 90 min at 37°C. RQ of each miRNA is compared with that of a no-treatment group. (B) RQ of each miRNA in exosomal miRNA treated with RNase. Exosomes are treated with 5 µg/mL of RNase for 0, 30, 60, and 90 min at 37°C. RQ of each group is compared with that of a no-treatment group. (C) RQ of each miRNA in fecal RNA treated with RNase. Fecal samples are treated with 5 µg/mL of RNase for 0, 30, 60, and 90 min at 37°C. RQ of each group is compared with that of a no-treatment group. Mean ± SD.
Techniques Used:
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