rnase (Qiagen)
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RNase A
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For RNase digestion during DNA preparation Kit contents Qiagen RNase A 17 500U 2 5mL 100mg mL Solution Endonuclease free Ready to use For RNase Digestion During DNA Preparation
Catalog Number:
19101
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RNase A
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Qiagen
rnase
For RNase digestion during DNA preparation Kit contents Qiagen RNase A 17 500U 2 5mL 100mg mL Solution Endonuclease free Ready to use For RNase Digestion During DNA Preparation
https://www.bioz.com/result/rnase/product/Qiagen
Average 90 stars, based on 87 article reviews
Price from $9.99 to $1999.99
For RNase digestion during DNA preparation Kit contents Qiagen RNase A 17 500U 2 5mL 100mg mL Solution Endonuclease free Ready to use For RNase Digestion During DNA Preparation
https://www.bioz.com/result/rnase/product/Qiagen
Average 90 stars, based on 87 article reviews
Price from $9.99 to $1999.99
rnase - by Bioz Stars,
2020-01
90/100 stars
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Clone Assay:Article Title: Mouse APOBEC3 Restricts Friend Leukemia Virus Infection and Pathogenesis In Vivo ▿ Article Snippet: F-MuLV in 1 ml of culture medium were inoculated onto a culture of Mus dunni cells and incubated for 18 h. After trypsinization and washing, the cells were treated with Centrifugation:Article Title: Cells adapt to the epigenomic disruption caused by histone deacetylase inhibitors through a coordinated, chromatin-mediated transcriptional response Article Snippet: Cells were harvested by centrifugation and RNA was extracted and purified using the RNeasy kit with DNase digestion (Qiagen) according to the manufacturer’s instructions. .. Double stranded cDNA was synthesised using the cDNA Synthesis System, including Article Title: Facile single-stranded DNA sequencing of human plasma DNA via thermostable group II intron reverse transcriptase template switching Article Snippet: After centrifugation, plasma (top layer) was transferred into a clean tube and divided into 1-ml portions, which were stored at −80 °C. .. To extract DNA, 400 μl of plasma was incubated with Article Title: Acute myeloid leukemia requires Hhex to enable PRC2-mediated epigenetic repression of Cdkn2a Article Snippet: Briefly, cells were fixed in 1.42% paraformaldehyde and lysed in immunoprecipitation buffer (1% Triton X-100, 0.5% NP40 in 50 mM Tris-HCl at pH 7.5, 150 mM NaCl, 5 mM EDTA, protease inhibitor cocktail), and the chromatin pellet was harvested by centrifugation. .. Following reversal of cross-links and Amplification:Article Title: Mouse APOBEC3 Restricts Friend Leukemia Virus Infection and Pathogenesis In Vivo ▿ Article Snippet: F-MuLV in 1 ml of culture medium were inoculated onto a culture of Mus dunni cells and incubated for 18 h. After trypsinization and washing, the cells were treated with Filtration:Article Title: Detection and genetic characterization of Seoul Virus from commensal brown rats in France Article Snippet: .. Condition S3 combined a step-wise filtration and a 2-step digestion with 25U of Cell Cycle Assay:Article Title: PTGER3 induces ovary tumorigenesis and confers resistance to cisplatin therapy through up-regulation Ras-MAPK/Erk-ETS1-ELK1/CFTR1 axis Article Snippet: Paragraph title: Apoptosis and cell cycle analysis ... The cells then were washed with cold phosphate-buffered saline (PBS), treated with 100 μg Cytometry:Article Title: PTGER3 induces ovary tumorigenesis and confers resistance to cisplatin therapy through up-regulation Ras-MAPK/Erk-ETS1-ELK1/CFTR1 axis Article Snippet: Apoptosis was analyzed by a FACSCalibur flow cytometer and CellQuest Pro software according to manufacturer's recommended protocol. .. The cells then were washed with cold phosphate-buffered saline (PBS), treated with 100 μg Real-time Polymerase Chain Reaction:Article Title: Epigenetic Regulation of Wnt Pathway Antagonists in Human Glioblastoma Multiforme Article Snippet: Nuclei isolated from TSA-treated cells (1 μM for 24 h) or control-treated (DMSO) T98 cells were suspended in 100 μL of digestion buffer (50 mM Tris [pH 8.0], 100 mM NaCl, 3 mM MgCl2 , 1 mM CaCl2 , 0.15 mM Spermine, and 0.5 mM spermidine) and 20 μg of Microarray:Article Title: Cells adapt to the epigenomic disruption caused by histone deacetylase inhibitors through a coordinated, chromatin-mediated transcriptional response Article Snippet: Paragraph title: Microarray expression analysis ... Double stranded cDNA was synthesised using the cDNA Synthesis System, including Article Title: Evaluation of a microarray-hybridization based method applicable for discovery of single nucleotide polymorphisms (SNPs) in the Pseudomonas aeruginosa genome Article Snippet: .. Cell lysates were treated with Article Title: ?-Lactam Resistance Response Triggered by Inactivation of a Nonessential Penicillin-Binding Protein Article Snippet: .. Cell lysates were treated with Incubation:Article Title: Facile single-stranded DNA sequencing of human plasma DNA via thermostable group II intron reverse transcriptase template switching Article Snippet: .. To extract DNA, 400 μl of plasma was incubated with Article Title: Nanopore sequencing and assembly of a human genome with ultra-long reads Article Snippet: .. 10 ml TLB was added (10 mM Tris-Cl pH 8.0, 25 mM EDTA pH 8.0, 0.5% (w/v) SDS, 20 μg/ml Article Title: Mouse APOBEC3 Restricts Friend Leukemia Virus Infection and Pathogenesis In Vivo ▿ Article Snippet: .. F-MuLV in 1 ml of culture medium were inoculated onto a culture of Mus dunni cells and incubated for 18 h. After trypsinization and washing, the cells were treated with Expressing:Article Title: Cells adapt to the epigenomic disruption caused by histone deacetylase inhibitors through a coordinated, chromatin-mediated transcriptional response Article Snippet: Paragraph title: Microarray expression analysis ... Double stranded cDNA was synthesised using the cDNA Synthesis System, including Article Title: The barrier-to-autointegration protein is a host factor for HIV type 1 integration Article Snippet: Human HMG I(Y) was purified from Escherichia coli cells as described ( ) after expression from plasmid pET7C ( ). .. For functional reconstitution of salt-stripped PICs, Article Title: Patterns of homoeologous gene expression shown by RNA sequencing in hexaploid bread wheat Article Snippet: Experimental validation of homoeologue sequence variants (HSVs) Two micrograms of total RNA extracted from euploid (CS) and nullitetra wheat root samples using the TRIzol Reagent (Invitrogen) was reverse-transcribed to synthesize first-strand cDNA using the SuperScript II First-Strand Synthesis System for RT-PCR Kit (Invitrogen). cDNA was treated with Modification:Article Title: Nanopore sequencing and assembly of a human genome with ultra-long reads Article Snippet: This protocol was modified from Chapter 6 protocol 1 of Sambrook and Russell . .. 10 ml TLB was added (10 mM Tris-Cl pH 8.0, 25 mM EDTA pH 8.0, 0.5% (w/v) SDS, 20 μg/ml Western Blot:Article Title: Scd6 targets eIF4G to repress translation: RGG-motif proteins as a class of eIF4G-binding proteins Article Snippet: Paragraph title: Protein purification, Cap-binding assays and Western blotting ... To remove RNA that might provide bridging interactions, extracts after breaking open the cells during the time of protein purification were treated for 20 min with Hybridization:Article Title: Cells adapt to the epigenomic disruption caused by histone deacetylase inhibitors through a coordinated, chromatin-mediated transcriptional response Article Snippet: Double stranded cDNA was synthesised using the cDNA Synthesis System, including Article Title: Evaluation of a microarray-hybridization based method applicable for discovery of single nucleotide polymorphisms (SNPs) in the Pseudomonas aeruginosa genome Article Snippet: Paragraph title: DNA preparation and Microarray hybridization ... Cell lysates were treated with Article Title: ?-Lactam Resistance Response Triggered by Inactivation of a Nonessential Penicillin-Binding Protein Article Snippet: Cell lysates were treated with Transfection:Article Title: PTGER3 induces ovary tumorigenesis and confers resistance to cisplatin therapy through up-regulation Ras-MAPK/Erk-ETS1-ELK1/CFTR1 axis Article Snippet: 2.7 Apoptosis and cell cycle analysis The resistant A2780-CP20 and OVCAR5 cell lines were transfected with non-silencing negative control or PTGER3 siRNA for 72 h and stained with the FITC-Apoptosis Detection Kit (BD Pharmingen) and propidium iodide (Molecular Probes). .. The cells then were washed with cold phosphate-buffered saline (PBS), treated with 100 μg Immunoprecipitation:Article Title: Acute myeloid leukemia requires Hhex to enable PRC2-mediated epigenetic repression of Cdkn2a Article Snippet: DNA was fragmented in a Covaris Sonicator for 30 min at 4°C, and then protein–DNA complexes were immunoprecipitated from clarified chromatin fractions using 2 µg of H3K27me3 (Millipore, 07-449) or 2 µg of H3K4me3 (Millipore, 07-473) antibodies and 30 µL of protein A-sepharose. .. Following reversal of cross-links and Protease Inhibitor:Article Title: Acute myeloid leukemia requires Hhex to enable PRC2-mediated epigenetic repression of Cdkn2a Article Snippet: Briefly, cells were fixed in 1.42% paraformaldehyde and lysed in immunoprecipitation buffer (1% Triton X-100, 0.5% NP40 in 50 mM Tris-HCl at pH 7.5, 150 mM NaCl, 5 mM EDTA, protease inhibitor cocktail), and the chromatin pellet was harvested by centrifugation. .. Following reversal of cross-links and Transferring:Article Title: Nanopore sequencing and assembly of a human genome with ultra-long reads Article Snippet: The cells were resuspended by pipette mixing in 100 μl PBS. .. 10 ml TLB was added (10 mM Tris-Cl pH 8.0, 25 mM EDTA pH 8.0, 0.5% (w/v) SDS, 20 μg/ml Infection:Article Title: Detection and genetic characterization of Seoul Virus from commensal brown rats in France Article Snippet: Viral enrichment and Illumina Hiseq 2500 v3 sequencing The different approaches for virus enrichment were also evaluated in this study for the infected lung tissue sample (LYO852) (Figure ). .. Condition S3 combined a step-wise filtration and a 2-step digestion with 25U of Polymerase Chain Reaction:Article Title: Cells adapt to the epigenomic disruption caused by histone deacetylase inhibitors through a coordinated, chromatin-mediated transcriptional response Article Snippet: .. Double stranded cDNA was synthesised using the cDNA Synthesis System, including Article Title: Mouse APOBEC3 Restricts Friend Leukemia Virus Infection and Pathogenesis In Vivo ▿ Article Snippet: F-MuLV in 1 ml of culture medium were inoculated onto a culture of Mus dunni cells and incubated for 18 h. After trypsinization and washing, the cells were treated with Article Title: Patterns of homoeologous gene expression shown by RNA sequencing in hexaploid bread wheat Article Snippet: Experimental validation of homoeologue sequence variants (HSVs) Two micrograms of total RNA extracted from euploid (CS) and nullitetra wheat root samples using the TRIzol Reagent (Invitrogen) was reverse-transcribed to synthesize first-strand cDNA using the SuperScript II First-Strand Synthesis System for RT-PCR Kit (Invitrogen). cDNA was treated with Transmission Assay:Article Title: Genome resequencing and transcriptome profiling reveal structural diversity and expression patterns of constitutive disease resistance genes in Huanglongbing-tolerant Poncirus trifoliata and its hybrids Article Snippet: The second source of DPI 50-7 and Flying Dragon plants was grown in the orchard at the University of Florida’s Citrus Research and Education Center in Lake Alfred, Florida, and had been exposed to C Las through natural psyllid transmission. .. Genomic DNA was extracted from the leaves using the CTAB method and treated with Reverse Transcription Polymerase Chain Reaction:Article Title: Patterns of homoeologous gene expression shown by RNA sequencing in hexaploid bread wheat Article Snippet: .. Experimental validation of homoeologue sequence variants (HSVs) Two micrograms of total RNA extracted from euploid (CS) and nullitetra wheat root samples using the TRIzol Reagent (Invitrogen) was reverse-transcribed to synthesize first-strand cDNA using the SuperScript II First-Strand Synthesis System for RT-PCR Kit (Invitrogen). cDNA was treated with Binding Assay:Article Title: Scd6 targets eIF4G to repress translation: RGG-motif proteins as a class of eIF4G-binding proteins Article Snippet: To remove RNA that might provide bridging interactions, extracts after breaking open the cells during the time of protein purification were treated for 20 min with DNA Extraction:Article Title: Nanopore sequencing and assembly of a human genome with ultra-long reads Article Snippet: Paragraph title: Sambrook and Russell DNA extraction ... 10 ml TLB was added (10 mM Tris-Cl pH 8.0, 25 mM EDTA pH 8.0, 0.5% (w/v) SDS, 20 μg/ml Nucleic Acid Electrophoresis:Article Title: Evaluation of a microarray-hybridization based method applicable for discovery of single nucleotide polymorphisms (SNPs) in the Pseudomonas aeruginosa genome Article Snippet: Cell lysates were treated with Article Title: ?-Lactam Resistance Response Triggered by Inactivation of a Nonessential Penicillin-Binding Protein Article Snippet: Cell lysates were treated with RNA Sequencing Assay:Article Title: Genome resequencing and transcriptome profiling reveal structural diversity and expression patterns of constitutive disease resistance genes in Huanglongbing-tolerant Poncirus trifoliata and its hybrids Article Snippet: Genomic DNA was extracted from the leaves using the CTAB method and treated with Isolation:Article Title: Evaluation of a microarray-hybridization based method applicable for discovery of single nucleotide polymorphisms (SNPs) in the Pseudomonas aeruginosa genome Article Snippet: Genomic DNA was isolated using the DNeasy Blood & Tissue Kit (Qiagen, Hamburg, Germany). .. Cell lysates were treated with Article Title: Facile single-stranded DNA sequencing of human plasma DNA via thermostable group II intron reverse transcriptase template switching Article Snippet: Preparation of human plasma DNA cfDNA was isolated from plasma of a healthy male individual obtained from the Genome Sequencing and Analysis Facility at the University of Texas at Austin. .. To extract DNA, 400 μl of plasma was incubated with Article Title: Mouse APOBEC3 Restricts Friend Leukemia Virus Infection and Pathogenesis In Vivo ▿ Article Snippet: .. F-MuLV in 1 ml of culture medium were inoculated onto a culture of Mus dunni cells and incubated for 18 h. After trypsinization and washing, the cells were treated with Article Title: ?-Lactam Resistance Response Triggered by Inactivation of a Nonessential Penicillin-Binding Protein Article Snippet: Cultures were grown in brain-heart infusion (BHI) medium for 12 h at 37°C in shaking glass flasks at 180 rpm and genomic DNA was isolated using the DNeasy Blood & Tissue Kit (Qiagen). .. Cell lysates were treated with Article Title: Epigenetic Regulation of Wnt Pathway Antagonists in Human Glioblastoma Multiforme Article Snippet: .. Nuclei isolated from TSA-treated cells (1 μM for 24 h) or control-treated (DMSO) T98 cells were suspended in 100 μL of digestion buffer (50 mM Tris [pH 8.0], 100 mM NaCl, 3 mM MgCl2 , 1 mM CaCl2 , 0.15 mM Spermine, and 0.5 mM spermidine) and 20 μg of Article Title: Genome resequencing and transcriptome profiling reveal structural diversity and expression patterns of constitutive disease resistance genes in Huanglongbing-tolerant Poncirus trifoliata and its hybrids Article Snippet: Paragraph title: Plant material, DNA and RNA isolation and HiSeq sequencing ... Genomic DNA was extracted from the leaves using the CTAB method and treated with Flow Cytometry:Article Title: PTGER3 induces ovary tumorigenesis and confers resistance to cisplatin therapy through up-regulation Ras-MAPK/Erk-ETS1-ELK1/CFTR1 axis Article Snippet: Apoptosis was analyzed by a FACSCalibur flow cytometer and CellQuest Pro software according to manufacturer's recommended protocol. .. The cells then were washed with cold phosphate-buffered saline (PBS), treated with 100 μg Labeling:Article Title: Cells adapt to the epigenomic disruption caused by histone deacetylase inhibitors through a coordinated, chromatin-mediated transcriptional response Article Snippet: Double stranded cDNA was synthesised using the cDNA Synthesis System, including Article Title: Evaluation of a microarray-hybridization based method applicable for discovery of single nucleotide polymorphisms (SNPs) in the Pseudomonas aeruginosa genome Article Snippet: Cell lysates were treated with Article Title: ?-Lactam Resistance Response Triggered by Inactivation of a Nonessential Penicillin-Binding Protein Article Snippet: Cell lysates were treated with Purification:Article Title: Cells adapt to the epigenomic disruption caused by histone deacetylase inhibitors through a coordinated, chromatin-mediated transcriptional response Article Snippet: .. Double stranded cDNA was synthesised using the cDNA Synthesis System, including Article Title: Acute myeloid leukemia requires Hhex to enable PRC2-mediated epigenetic repression of Cdkn2a Article Snippet: .. Following reversal of cross-links and Article Title: Scd6 targets eIF4G to repress translation: RGG-motif proteins as a class of eIF4G-binding proteins Article Snippet: Proteins were purified from E. coli according to standard protocols using glutathione sepharose beads (GE), Ni-NTA agarose (Qiagen) or FLAG-agarose (Sigma-Aldrich) according to standard protocols. .. To remove RNA that might provide bridging interactions, extracts after breaking open the cells during the time of protein purification were treated for 20 min with Article Title: The barrier-to-autointegration protein is a host factor for HIV type 1 integration Article Snippet: Human HMG I(Y) was purified from Escherichia coli cells as described ( ) after expression from plasmid pET7C ( ). .. For functional reconstitution of salt-stripped PICs, Article Title: Epigenetic Regulation of Wnt Pathway Antagonists in Human Glioblastoma Multiforme Article Snippet: Nuclei isolated from TSA-treated cells (1 μM for 24 h) or control-treated (DMSO) T98 cells were suspended in 100 μL of digestion buffer (50 mM Tris [pH 8.0], 100 mM NaCl, 3 mM MgCl2 , 1 mM CaCl2 , 0.15 mM Spermine, and 0.5 mM spermidine) and 20 μg of Protein Purification:Article Title: Scd6 targets eIF4G to repress translation: RGG-motif proteins as a class of eIF4G-binding proteins Article Snippet: .. To remove RNA that might provide bridging interactions, extracts after breaking open the cells during the time of protein purification were treated for 20 min with Sequencing:Article Title: Cells adapt to the epigenomic disruption caused by histone deacetylase inhibitors through a coordinated, chromatin-mediated transcriptional response Article Snippet: Double stranded cDNA was synthesised using the cDNA Synthesis System, including Article Title: Facile single-stranded DNA sequencing of human plasma DNA via thermostable group II intron reverse transcriptase template switching Article Snippet: Preparation of human plasma DNA cfDNA was isolated from plasma of a healthy male individual obtained from the Genome Sequencing and Analysis Facility at the University of Texas at Austin. .. To extract DNA, 400 μl of plasma was incubated with Article Title: Mouse APOBEC3 Restricts Friend Leukemia Virus Infection and Pathogenesis In Vivo ▿ Article Snippet: Paragraph title: Sequence analysis of proviral DNA. ... F-MuLV in 1 ml of culture medium were inoculated onto a culture of Mus dunni cells and incubated for 18 h. After trypsinization and washing, the cells were treated with Article Title: Detection and genetic characterization of Seoul Virus from commensal brown rats in France Article Snippet: Paragraph title: Viral enrichment and Illumina Hiseq 2500 v3 sequencing ... Condition S3 combined a step-wise filtration and a 2-step digestion with 25U of Article Title: Patterns of homoeologous gene expression shown by RNA sequencing in hexaploid bread wheat Article Snippet: .. Experimental validation of homoeologue sequence variants (HSVs) Two micrograms of total RNA extracted from euploid (CS) and nullitetra wheat root samples using the TRIzol Reagent (Invitrogen) was reverse-transcribed to synthesize first-strand cDNA using the SuperScript II First-Strand Synthesis System for RT-PCR Kit (Invitrogen). cDNA was treated with Article Title: Genome resequencing and transcriptome profiling reveal structural diversity and expression patterns of constitutive disease resistance genes in Huanglongbing-tolerant Poncirus trifoliata and its hybrids Article Snippet: Paragraph title: Plant material, DNA and RNA isolation and HiSeq sequencing ... Genomic DNA was extracted from the leaves using the CTAB method and treated with Chromatin Immunoprecipitation:Article Title: Acute myeloid leukemia requires Hhex to enable PRC2-mediated epigenetic repression of Cdkn2a Article Snippet: Paragraph title: ChIP ... Following reversal of cross-links and RNA Extraction:Article Title: Detection and genetic characterization of Seoul Virus from commensal brown rats in France Article Snippet: The resulting supernatant was transferred to a new tube and 2 different treatments were applied: for the condition S2 a step-wise filtration process involving 0.45 μm polyethersulfone membrane filters (diameter 13 mm) (Millipore) was performed before RNA extraction. .. Condition S3 combined a step-wise filtration and a 2-step digestion with 25U of Plasmid Preparation:Article Title: The barrier-to-autointegration protein is a host factor for HIV type 1 integration Article Snippet: Human HMG I(Y) was purified from Escherichia coli cells as described ( ) after expression from plasmid pET7C ( ). .. For functional reconstitution of salt-stripped PICs, Article Title: Mouse APOBEC3 Restricts Friend Leukemia Virus Infection and Pathogenesis In Vivo ▿ Article Snippet: F-MuLV in 1 ml of culture medium were inoculated onto a culture of Mus dunni cells and incubated for 18 h. After trypsinization and washing, the cells were treated with Software:Article Title: PTGER3 induces ovary tumorigenesis and confers resistance to cisplatin therapy through up-regulation Ras-MAPK/Erk-ETS1-ELK1/CFTR1 axis Article Snippet: Apoptosis was analyzed by a FACSCalibur flow cytometer and CellQuest Pro software according to manufacturer's recommended protocol. .. The cells then were washed with cold phosphate-buffered saline (PBS), treated with 100 μg Negative Control:Article Title: PTGER3 induces ovary tumorigenesis and confers resistance to cisplatin therapy through up-regulation Ras-MAPK/Erk-ETS1-ELK1/CFTR1 axis Article Snippet: 2.7 Apoptosis and cell cycle analysis The resistant A2780-CP20 and OVCAR5 cell lines were transfected with non-silencing negative control or PTGER3 siRNA for 72 h and stained with the FITC-Apoptosis Detection Kit (BD Pharmingen) and propidium iodide (Molecular Probes). .. The cells then were washed with cold phosphate-buffered saline (PBS), treated with 100 μg Recombinant:Article Title: The barrier-to-autointegration protein is a host factor for HIV type 1 integration Article Snippet: Purified recombinant human BAF was a generous gift from Robert Craigie, National Institute of Diabetes and Digestive and Kidney Diseases. .. For functional reconstitution of salt-stripped PICs, Sample Prep:Article Title: Genome resequencing and transcriptome profiling reveal structural diversity and expression patterns of constitutive disease resistance genes in Huanglongbing-tolerant Poncirus trifoliata and its hybrids Article Snippet: Genomic DNA was extracted from the leaves using the CTAB method and treated with Functional Assay:Article Title: Cells adapt to the epigenomic disruption caused by histone deacetylase inhibitors through a coordinated, chromatin-mediated transcriptional response Article Snippet: Double stranded cDNA was synthesised using the cDNA Synthesis System, including Article Title: The barrier-to-autointegration protein is a host factor for HIV type 1 integration Article Snippet: .. For functional reconstitution of salt-stripped PICs, Concentration Assay:Article Title: Detection and genetic characterization of Seoul Virus from commensal brown rats in France Article Snippet: Condition S3 combined a step-wise filtration and a 2-step digestion with 25U of Staining:Article Title: Evaluation of a microarray-hybridization based method applicable for discovery of single nucleotide polymorphisms (SNPs) in the Pseudomonas aeruginosa genome Article Snippet: Cell lysates were treated with Article Title: PTGER3 induces ovary tumorigenesis and confers resistance to cisplatin therapy through up-regulation Ras-MAPK/Erk-ETS1-ELK1/CFTR1 axis Article Snippet: .. The cells then were washed with cold phosphate-buffered saline (PBS), treated with 100 μg Article Title: ?-Lactam Resistance Response Triggered by Inactivation of a Nonessential Penicillin-Binding Protein Article Snippet: Cell lysates were treated with |