rnase  (Qiagen)


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    Name:
    RNase A
    Description:
    For RNase digestion during DNA preparation Kit contents Qiagen RNase A 17 500U 2 5mL 100mg mL Solution Endonuclease free Ready to use For RNase Digestion During DNA Preparation
    Catalog Number:
    19101
    Price:
    215
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    RNase A
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    Qiagen rnase
    RNase A
    For RNase digestion during DNA preparation Kit contents Qiagen RNase A 17 500U 2 5mL 100mg mL Solution Endonuclease free Ready to use For RNase Digestion During DNA Preparation
    https://www.bioz.com/result/rnase/product/Qiagen
    Average 90 stars, based on 87 article reviews
    Price from $9.99 to $1999.99
    rnase - by Bioz Stars, 2020-01
    90/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Mouse APOBEC3 Restricts Friend Leukemia Virus Infection and Pathogenesis In Vivo ▿
    Article Snippet: F-MuLV in 1 ml of culture medium were inoculated onto a culture of Mus dunni cells and incubated for 18 h. After trypsinization and washing, the cells were treated with RNase I, and their DNA was isolated by using DNeasy (Qiagen, Hilden, Germany). .. The amplified fragments were cloned into the pCR-Blunt vector using a Zero Blunt TOPO PCR cloning kit (Invitrogen).

    Centrifugation:

    Article Title: Cells adapt to the epigenomic disruption caused by histone deacetylase inhibitors through a coordinated, chromatin-mediated transcriptional response
    Article Snippet: Cells were harvested by centrifugation and RNA was extracted and purified using the RNeasy kit with DNase digestion (Qiagen) according to the manufacturer’s instructions. .. Double stranded cDNA was synthesised using the cDNA Synthesis System, including RNase I and Proteinase K treatment followed by DNA clean up using the PCR purification kit (Qiagen).

    Article Title: Facile single-stranded DNA sequencing of human plasma DNA via thermostable group II intron reverse transcriptase template switching
    Article Snippet: After centrifugation, plasma (top layer) was transferred into a clean tube and divided into 1-ml portions, which were stored at −80 °C. .. To extract DNA, 400 μl of plasma was incubated with RNase I (Qiagen; 800 μg) as suggested by the manufacturer’s protocol and then processed by using a Qiagen DSP DNA Blood Mini Kit (Qiagen).

    Article Title: Acute myeloid leukemia requires Hhex to enable PRC2-mediated epigenetic repression of Cdkn2a
    Article Snippet: Briefly, cells were fixed in 1.42% paraformaldehyde and lysed in immunoprecipitation buffer (1% Triton X-100, 0.5% NP40 in 50 mM Tris-HCl at pH 7.5, 150 mM NaCl, 5 mM EDTA, protease inhibitor cocktail), and the chromatin pellet was harvested by centrifugation. .. Following reversal of cross-links and RNase I and proteinase K treatments, extracted DNA was purified using the QIAquick purification kit (Qiagen).

    Amplification:

    Article Title: Mouse APOBEC3 Restricts Friend Leukemia Virus Infection and Pathogenesis In Vivo ▿
    Article Snippet: F-MuLV in 1 ml of culture medium were inoculated onto a culture of Mus dunni cells and incubated for 18 h. After trypsinization and washing, the cells were treated with RNase I, and their DNA was isolated by using DNeasy (Qiagen, Hilden, Germany). .. A 1.2-kbp fragment of the F-MuLV proviral genome harboring the U3 and a part of the gag sequence was amplified by PCR using the primers 5′-CGGGATCCAAGGACCTGAAATGACCCTG-3′ and 5′-GAAGAGAGAGGGGAGGTTTAGGG-3′.

    Filtration:

    Article Title: Detection and genetic characterization of Seoul Virus from commensal brown rats in France
    Article Snippet: .. Condition S3 combined a step-wise filtration and a 2-step digestion with 25U of RNase I at 37°C for 90 min in 1× RNase I buffer and DNA is removed on-column (Qiagen). .. Following treatment, we extracted viral encapsidated RNA and residual host nucleic acids using the RNeasy mini extraction kit (Qiagen).

    Cell Cycle Assay:

    Article Title: PTGER3 induces ovary tumorigenesis and confers resistance to cisplatin therapy through up-regulation Ras-MAPK/Erk-ETS1-ELK1/CFTR1 axis
    Article Snippet: Paragraph title: Apoptosis and cell cycle analysis ... The cells then were washed with cold phosphate-buffered saline (PBS), treated with 100 μg of RNase A (Qiagen), and stained with 50 μg of propidium iodide.

    Cytometry:

    Article Title: PTGER3 induces ovary tumorigenesis and confers resistance to cisplatin therapy through up-regulation Ras-MAPK/Erk-ETS1-ELK1/CFTR1 axis
    Article Snippet: Apoptosis was analyzed by a FACSCalibur flow cytometer and CellQuest Pro software according to manufacturer's recommended protocol. .. The cells then were washed with cold phosphate-buffered saline (PBS), treated with 100 μg of RNase A (Qiagen), and stained with 50 μg of propidium iodide.

    Real-time Polymerase Chain Reaction:

    Article Title: Epigenetic Regulation of Wnt Pathway Antagonists in Human Glioblastoma Multiforme
    Article Snippet: Nuclei isolated from TSA-treated cells (1 μM for 24 h) or control-treated (DMSO) T98 cells were suspended in 100 μL of digestion buffer (50 mM Tris [pH 8.0], 100 mM NaCl, 3 mM MgCl2 , 1 mM CaCl2 , 0.15 mM Spermine, and 0.5 mM spermidine) and 20 μg of RNAse I (Qiagen). .. Real-time PCR was performed using a primer pair specific for DKK1, SFRP1, or WIF1 promoter regions to determine the number of target sequences after DNase I digestion.

    Microarray:

    Article Title: Cells adapt to the epigenomic disruption caused by histone deacetylase inhibitors through a coordinated, chromatin-mediated transcriptional response
    Article Snippet: Paragraph title: Microarray expression analysis ... Double stranded cDNA was synthesised using the cDNA Synthesis System, including RNase I and Proteinase K treatment followed by DNA clean up using the PCR purification kit (Qiagen).

    Article Title: Evaluation of a microarray-hybridization based method applicable for discovery of single nucleotide polymorphisms (SNPs) in the Pseudomonas aeruginosa genome
    Article Snippet: .. Cell lysates were treated with RNase I (Qiagen) to prevent accidental carryover of RNA to the microarray. .. Genomic DNA was partially digested with DNase I (Amersham Biosciences, Piscataway, NJ) to a fragment size of ~50 – 250 bp, confirmed by gel electrophoresis, and fragments were labeled at the 3'-ends with biotin-ddUTP (Roche Diagnostics, Indianapolis, IN) using Terminal deoxynucleotidyl transferase (Roche).

    Article Title: ?-Lactam Resistance Response Triggered by Inactivation of a Nonessential Penicillin-Binding Protein
    Article Snippet: .. Cell lysates were treated with RNase I (Qiagen) to prevent accidental carryover of RNA to the microarray. .. Genomic DNA was partially digested with DNase I (Amersham Biosciences, Piscataway, NJ) to a fragment size of ∼50–250 bp, confirmed by gel electrophoresis, and fragments were labeled at the 3′-ends with biotin-ddUTP (Roche Diagnostics, Indianapolis, IN) using Terminal deoxynucleotidyl transferase (Roche).

    Incubation:

    Article Title: Facile single-stranded DNA sequencing of human plasma DNA via thermostable group II intron reverse transcriptase template switching
    Article Snippet: .. To extract DNA, 400 μl of plasma was incubated with RNase I (Qiagen; 800 μg) as suggested by the manufacturer’s protocol and then processed by using a Qiagen DSP DNA Blood Mini Kit (Qiagen). .. The products were mixed and concentrated with an Oligo C lean and Concentrator kit (Zymo) and eluted in 10 μl of water per 1 ml plasma.

    Article Title: Nanopore sequencing and assembly of a human genome with ultra-long reads
    Article Snippet: .. 10 ml TLB was added (10 mM Tris-Cl pH 8.0, 25 mM EDTA pH 8.0, 0.5% (w/v) SDS, 20 μg/ml Qiagen RNase A), vortexed at full speed for 5 s and incubated at 37 °C for 1 h. 50 μl Proteinase K (Qiagen) was added and mixed by slow inversion ten times followed by 3 h at 50 °C with gentle mixing every 1 h. The lysate was phenol-purified using 10 ml buffer saturated phenol using phase-lock gel falcon tubes, followed by phenol:chloroform (1:1). ..

    Article Title: Mouse APOBEC3 Restricts Friend Leukemia Virus Infection and Pathogenesis In Vivo ▿
    Article Snippet: .. F-MuLV in 1 ml of culture medium were inoculated onto a culture of Mus dunni cells and incubated for 18 h. After trypsinization and washing, the cells were treated with RNase I, and their DNA was isolated by using DNeasy (Qiagen, Hilden, Germany). .. A 1.2-kbp fragment of the F-MuLV proviral genome harboring the U3 and a part of the gag sequence was amplified by PCR using the primers 5′-CGGGATCCAAGGACCTGAAATGACCCTG-3′ and 5′-GAAGAGAGAGGGGAGGTTTAGGG-3′.

    Expressing:

    Article Title: Cells adapt to the epigenomic disruption caused by histone deacetylase inhibitors through a coordinated, chromatin-mediated transcriptional response
    Article Snippet: Paragraph title: Microarray expression analysis ... Double stranded cDNA was synthesised using the cDNA Synthesis System, including RNase I and Proteinase K treatment followed by DNA clean up using the PCR purification kit (Qiagen).

    Article Title: The barrier-to-autointegration protein is a host factor for HIV type 1 integration
    Article Snippet: Human HMG I(Y) was purified from Escherichia coli cells as described ( ) after expression from plasmid pET7C ( ). .. For functional reconstitution of salt-stripped PICs, Qiagen RNase A was used.

    Article Title: Patterns of homoeologous gene expression shown by RNA sequencing in hexaploid bread wheat
    Article Snippet: Experimental validation of homoeologue sequence variants (HSVs) Two micrograms of total RNA extracted from euploid (CS) and nullitetra wheat root samples using the TRIzol Reagent (Invitrogen) was reverse-transcribed to synthesize first-strand cDNA using the SuperScript II First-Strand Synthesis System for RT-PCR Kit (Invitrogen). cDNA was treated with Qiagen RNase A to remove any residual RNA. .. We selected 10 genes with differential expression of homoeoloci and with three homoeolocus-specific tentative contigs (TCs) identified from a BLAST search of the T. aestivum TIGR database (Additional file : Table S4A).

    Modification:

    Article Title: Nanopore sequencing and assembly of a human genome with ultra-long reads
    Article Snippet: This protocol was modified from Chapter 6 protocol 1 of Sambrook and Russell . .. 10 ml TLB was added (10 mM Tris-Cl pH 8.0, 25 mM EDTA pH 8.0, 0.5% (w/v) SDS, 20 μg/ml Qiagen RNase A), vortexed at full speed for 5 s and incubated at 37 °C for 1 h. 50 μl Proteinase K (Qiagen) was added and mixed by slow inversion ten times followed by 3 h at 50 °C with gentle mixing every 1 h. The lysate was phenol-purified using 10 ml buffer saturated phenol using phase-lock gel falcon tubes, followed by phenol:chloroform (1:1).

    Western Blot:

    Article Title: Scd6 targets eIF4G to repress translation: RGG-motif proteins as a class of eIF4G-binding proteins
    Article Snippet: Paragraph title: Protein purification, Cap-binding assays and Western blotting ... To remove RNA that might provide bridging interactions, extracts after breaking open the cells during the time of protein purification were treated for 20 min with Qiagen RNase A (1mg/ml).

    Hybridization:

    Article Title: Cells adapt to the epigenomic disruption caused by histone deacetylase inhibitors through a coordinated, chromatin-mediated transcriptional response
    Article Snippet: Double stranded cDNA was synthesised using the cDNA Synthesis System, including RNase I and Proteinase K treatment followed by DNA clean up using the PCR purification kit (Qiagen). .. Samples were labelled with cy3 using a Nimblegen One-Colour Labeling Kit, mixed with alignment oligos and sample tracking control oligos (Nimblegen Hybridisation and Sample Tracking Control Kits) and hybridised to a 12 × 135k HD2 expression array (Roche Nimblegen, containing 3 probes per sequence for 44,049 human sequences) and scanned on a Nimblegen MS200 Microarray scanner.

    Article Title: Evaluation of a microarray-hybridization based method applicable for discovery of single nucleotide polymorphisms (SNPs) in the Pseudomonas aeruginosa genome
    Article Snippet: Paragraph title: DNA preparation and Microarray hybridization ... Cell lysates were treated with RNase I (Qiagen) to prevent accidental carryover of RNA to the microarray.

    Article Title: ?-Lactam Resistance Response Triggered by Inactivation of a Nonessential Penicillin-Binding Protein
    Article Snippet: Cell lysates were treated with RNase I (Qiagen) to prevent accidental carryover of RNA to the microarray. .. After hybridization the microarrays were washed, stained with SA-PE and read using an Affymetrix GeneChip fluidic station and scanner according to Affymetrix standard protocols (Affymetrix, Santa Clara, CA).

    Transfection:

    Article Title: PTGER3 induces ovary tumorigenesis and confers resistance to cisplatin therapy through up-regulation Ras-MAPK/Erk-ETS1-ELK1/CFTR1 axis
    Article Snippet: 2.7 Apoptosis and cell cycle analysis The resistant A2780-CP20 and OVCAR5 cell lines were transfected with non-silencing negative control or PTGER3 siRNA for 72 h and stained with the FITC-Apoptosis Detection Kit (BD Pharmingen) and propidium iodide (Molecular Probes). .. The cells then were washed with cold phosphate-buffered saline (PBS), treated with 100 μg of RNase A (Qiagen), and stained with 50 μg of propidium iodide.

    Immunoprecipitation:

    Article Title: Acute myeloid leukemia requires Hhex to enable PRC2-mediated epigenetic repression of Cdkn2a
    Article Snippet: DNA was fragmented in a Covaris Sonicator for 30 min at 4°C, and then protein–DNA complexes were immunoprecipitated from clarified chromatin fractions using 2 µg of H3K27me3 (Millipore, 07-449) or 2 µg of H3K4me3 (Millipore, 07-473) antibodies and 30 µL of protein A-sepharose. .. Following reversal of cross-links and RNase I and proteinase K treatments, extracted DNA was purified using the QIAquick purification kit (Qiagen).

    Protease Inhibitor:

    Article Title: Acute myeloid leukemia requires Hhex to enable PRC2-mediated epigenetic repression of Cdkn2a
    Article Snippet: Briefly, cells were fixed in 1.42% paraformaldehyde and lysed in immunoprecipitation buffer (1% Triton X-100, 0.5% NP40 in 50 mM Tris-HCl at pH 7.5, 150 mM NaCl, 5 mM EDTA, protease inhibitor cocktail), and the chromatin pellet was harvested by centrifugation. .. Following reversal of cross-links and RNase I and proteinase K treatments, extracted DNA was purified using the QIAquick purification kit (Qiagen).

    Transferring:

    Article Title: Nanopore sequencing and assembly of a human genome with ultra-long reads
    Article Snippet: The cells were resuspended by pipette mixing in 100 μl PBS. .. 10 ml TLB was added (10 mM Tris-Cl pH 8.0, 25 mM EDTA pH 8.0, 0.5% (w/v) SDS, 20 μg/ml Qiagen RNase A), vortexed at full speed for 5 s and incubated at 37 °C for 1 h. 50 μl Proteinase K (Qiagen) was added and mixed by slow inversion ten times followed by 3 h at 50 °C with gentle mixing every 1 h. The lysate was phenol-purified using 10 ml buffer saturated phenol using phase-lock gel falcon tubes, followed by phenol:chloroform (1:1).

    Infection:

    Article Title: Detection and genetic characterization of Seoul Virus from commensal brown rats in France
    Article Snippet: Viral enrichment and Illumina Hiseq 2500 v3 sequencing The different approaches for virus enrichment were also evaluated in this study for the infected lung tissue sample (LYO852) (Figure ). .. Condition S3 combined a step-wise filtration and a 2-step digestion with 25U of RNase I at 37°C for 90 min in 1× RNase I buffer and DNA is removed on-column (Qiagen).

    Polymerase Chain Reaction:

    Article Title: Cells adapt to the epigenomic disruption caused by histone deacetylase inhibitors through a coordinated, chromatin-mediated transcriptional response
    Article Snippet: .. Double stranded cDNA was synthesised using the cDNA Synthesis System, including RNase I and Proteinase K treatment followed by DNA clean up using the PCR purification kit (Qiagen). .. Samples were labelled with cy3 using a Nimblegen One-Colour Labeling Kit, mixed with alignment oligos and sample tracking control oligos (Nimblegen Hybridisation and Sample Tracking Control Kits) and hybridised to a 12 × 135k HD2 expression array (Roche Nimblegen, containing 3 probes per sequence for 44,049 human sequences) and scanned on a Nimblegen MS200 Microarray scanner.

    Article Title: Mouse APOBEC3 Restricts Friend Leukemia Virus Infection and Pathogenesis In Vivo ▿
    Article Snippet: F-MuLV in 1 ml of culture medium were inoculated onto a culture of Mus dunni cells and incubated for 18 h. After trypsinization and washing, the cells were treated with RNase I, and their DNA was isolated by using DNeasy (Qiagen, Hilden, Germany). .. A 1.2-kbp fragment of the F-MuLV proviral genome harboring the U3 and a part of the gag sequence was amplified by PCR using the primers 5′-CGGGATCCAAGGACCTGAAATGACCCTG-3′ and 5′-GAAGAGAGAGGGGAGGTTTAGGG-3′.

    Article Title: Patterns of homoeologous gene expression shown by RNA sequencing in hexaploid bread wheat
    Article Snippet: Experimental validation of homoeologue sequence variants (HSVs) Two micrograms of total RNA extracted from euploid (CS) and nullitetra wheat root samples using the TRIzol Reagent (Invitrogen) was reverse-transcribed to synthesize first-strand cDNA using the SuperScript II First-Strand Synthesis System for RT-PCR Kit (Invitrogen). cDNA was treated with Qiagen RNase A to remove any residual RNA. .. All PCR primers were designed using Primer 3 ( http://frodo.wi.mit.edu/ ) to amplify either all three homoeoloci (using common forward and reverse primers) or specific homoeoloci (using homoeolocus-specific reverse primers).

    Transmission Assay:

    Article Title: Genome resequencing and transcriptome profiling reveal structural diversity and expression patterns of constitutive disease resistance genes in Huanglongbing-tolerant Poncirus trifoliata and its hybrids
    Article Snippet: The second source of DPI 50-7 and Flying Dragon plants was grown in the orchard at the University of Florida’s Citrus Research and Education Center in Lake Alfred, Florida, and had been exposed to C Las through natural psyllid transmission. .. Genomic DNA was extracted from the leaves using the CTAB method and treated with RNase I (Qiagen, Hilden, Germany).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Patterns of homoeologous gene expression shown by RNA sequencing in hexaploid bread wheat
    Article Snippet: .. Experimental validation of homoeologue sequence variants (HSVs) Two micrograms of total RNA extracted from euploid (CS) and nullitetra wheat root samples using the TRIzol Reagent (Invitrogen) was reverse-transcribed to synthesize first-strand cDNA using the SuperScript II First-Strand Synthesis System for RT-PCR Kit (Invitrogen). cDNA was treated with Qiagen RNase A to remove any residual RNA. .. We selected 10 genes with differential expression of homoeoloci and with three homoeolocus-specific tentative contigs (TCs) identified from a BLAST search of the T. aestivum TIGR database (Additional file : Table S4A).

    Binding Assay:

    Article Title: Scd6 targets eIF4G to repress translation: RGG-motif proteins as a class of eIF4G-binding proteins
    Article Snippet: To remove RNA that might provide bridging interactions, extracts after breaking open the cells during the time of protein purification were treated for 20 min with Qiagen RNase A (1mg/ml). .. Binding reactions for m7-GTP sepharose pull downs and GST-pull downs were performed at 4°C in binding buffer (50 mM HEPES, pH 7.4, 100 mM NaCl, 2 mM DTT, 2 mM MnCl2 , 2 mM MgCl2 , 1% Igepal CA-630 (USB), 10% glycerol, and 10 mg/ml BSA) containing 25 ng/μl of the target protein (without GST tag) and 25 ng/μl of the GST tagged bait protein.

    DNA Extraction:

    Article Title: Nanopore sequencing and assembly of a human genome with ultra-long reads
    Article Snippet: Paragraph title: Sambrook and Russell DNA extraction ... 10 ml TLB was added (10 mM Tris-Cl pH 8.0, 25 mM EDTA pH 8.0, 0.5% (w/v) SDS, 20 μg/ml Qiagen RNase A), vortexed at full speed for 5 s and incubated at 37 °C for 1 h. 50 μl Proteinase K (Qiagen) was added and mixed by slow inversion ten times followed by 3 h at 50 °C with gentle mixing every 1 h. The lysate was phenol-purified using 10 ml buffer saturated phenol using phase-lock gel falcon tubes, followed by phenol:chloroform (1:1).

    Nucleic Acid Electrophoresis:

    Article Title: Evaluation of a microarray-hybridization based method applicable for discovery of single nucleotide polymorphisms (SNPs) in the Pseudomonas aeruginosa genome
    Article Snippet: Cell lysates were treated with RNase I (Qiagen) to prevent accidental carryover of RNA to the microarray. .. Genomic DNA was partially digested with DNase I (Amersham Biosciences, Piscataway, NJ) to a fragment size of ~50 – 250 bp, confirmed by gel electrophoresis, and fragments were labeled at the 3'-ends with biotin-ddUTP (Roche Diagnostics, Indianapolis, IN) using Terminal deoxynucleotidyl transferase (Roche).

    Article Title: ?-Lactam Resistance Response Triggered by Inactivation of a Nonessential Penicillin-Binding Protein
    Article Snippet: Cell lysates were treated with RNase I (Qiagen) to prevent accidental carryover of RNA to the microarray. .. Genomic DNA was partially digested with DNase I (Amersham Biosciences, Piscataway, NJ) to a fragment size of ∼50–250 bp, confirmed by gel electrophoresis, and fragments were labeled at the 3′-ends with biotin-ddUTP (Roche Diagnostics, Indianapolis, IN) using Terminal deoxynucleotidyl transferase (Roche).

    RNA Sequencing Assay:

    Article Title: Genome resequencing and transcriptome profiling reveal structural diversity and expression patterns of constitutive disease resistance genes in Huanglongbing-tolerant Poncirus trifoliata and its hybrids
    Article Snippet: Genomic DNA was extracted from the leaves using the CTAB method and treated with RNase I (Qiagen, Hilden, Germany). .. RNA samples were shipped to Novogene Corporation where mRNA were enriched and converted into cDNAs, which were sequenced on an Illumina HiSeq 2500 using the Illumina ‘TruSeq RNA-Seq Sample Prep kit.’

    Isolation:

    Article Title: Evaluation of a microarray-hybridization based method applicable for discovery of single nucleotide polymorphisms (SNPs) in the Pseudomonas aeruginosa genome
    Article Snippet: Genomic DNA was isolated using the DNeasy Blood & Tissue Kit (Qiagen, Hamburg, Germany). .. Cell lysates were treated with RNase I (Qiagen) to prevent accidental carryover of RNA to the microarray.

    Article Title: Facile single-stranded DNA sequencing of human plasma DNA via thermostable group II intron reverse transcriptase template switching
    Article Snippet: Preparation of human plasma DNA cfDNA was isolated from plasma of a healthy male individual obtained from the Genome Sequencing and Analysis Facility at the University of Texas at Austin. .. To extract DNA, 400 μl of plasma was incubated with RNase I (Qiagen; 800 μg) as suggested by the manufacturer’s protocol and then processed by using a Qiagen DSP DNA Blood Mini Kit (Qiagen).

    Article Title: Mouse APOBEC3 Restricts Friend Leukemia Virus Infection and Pathogenesis In Vivo ▿
    Article Snippet: .. F-MuLV in 1 ml of culture medium were inoculated onto a culture of Mus dunni cells and incubated for 18 h. After trypsinization and washing, the cells were treated with RNase I, and their DNA was isolated by using DNeasy (Qiagen, Hilden, Germany). .. A 1.2-kbp fragment of the F-MuLV proviral genome harboring the U3 and a part of the gag sequence was amplified by PCR using the primers 5′-CGGGATCCAAGGACCTGAAATGACCCTG-3′ and 5′-GAAGAGAGAGGGGAGGTTTAGGG-3′.

    Article Title: ?-Lactam Resistance Response Triggered by Inactivation of a Nonessential Penicillin-Binding Protein
    Article Snippet: Cultures were grown in brain-heart infusion (BHI) medium for 12 h at 37°C in shaking glass flasks at 180 rpm and genomic DNA was isolated using the DNeasy Blood & Tissue Kit (Qiagen). .. Cell lysates were treated with RNase I (Qiagen) to prevent accidental carryover of RNA to the microarray.

    Article Title: Epigenetic Regulation of Wnt Pathway Antagonists in Human Glioblastoma Multiforme
    Article Snippet: .. Nuclei isolated from TSA-treated cells (1 μM for 24 h) or control-treated (DMSO) T98 cells were suspended in 100 μL of digestion buffer (50 mM Tris [pH 8.0], 100 mM NaCl, 3 mM MgCl2 , 1 mM CaCl2 , 0.15 mM Spermine, and 0.5 mM spermidine) and 20 μg of RNAse I (Qiagen). .. Nuclease digestion was carried out by adding 20 U of RQ1 DNase (Promega, Madison, WI) and incubating for 2 min at 37°C.

    Article Title: Genome resequencing and transcriptome profiling reveal structural diversity and expression patterns of constitutive disease resistance genes in Huanglongbing-tolerant Poncirus trifoliata and its hybrids
    Article Snippet: Paragraph title: Plant material, DNA and RNA isolation and HiSeq sequencing ... Genomic DNA was extracted from the leaves using the CTAB method and treated with RNase I (Qiagen, Hilden, Germany).

    Flow Cytometry:

    Article Title: PTGER3 induces ovary tumorigenesis and confers resistance to cisplatin therapy through up-regulation Ras-MAPK/Erk-ETS1-ELK1/CFTR1 axis
    Article Snippet: Apoptosis was analyzed by a FACSCalibur flow cytometer and CellQuest Pro software according to manufacturer's recommended protocol. .. The cells then were washed with cold phosphate-buffered saline (PBS), treated with 100 μg of RNase A (Qiagen), and stained with 50 μg of propidium iodide.

    Labeling:

    Article Title: Cells adapt to the epigenomic disruption caused by histone deacetylase inhibitors through a coordinated, chromatin-mediated transcriptional response
    Article Snippet: Double stranded cDNA was synthesised using the cDNA Synthesis System, including RNase I and Proteinase K treatment followed by DNA clean up using the PCR purification kit (Qiagen). .. Samples were labelled with cy3 using a Nimblegen One-Colour Labeling Kit, mixed with alignment oligos and sample tracking control oligos (Nimblegen Hybridisation and Sample Tracking Control Kits) and hybridised to a 12 × 135k HD2 expression array (Roche Nimblegen, containing 3 probes per sequence for 44,049 human sequences) and scanned on a Nimblegen MS200 Microarray scanner.

    Article Title: Evaluation of a microarray-hybridization based method applicable for discovery of single nucleotide polymorphisms (SNPs) in the Pseudomonas aeruginosa genome
    Article Snippet: Cell lysates were treated with RNase I (Qiagen) to prevent accidental carryover of RNA to the microarray. .. Genomic DNA was partially digested with DNase I (Amersham Biosciences, Piscataway, NJ) to a fragment size of ~50 – 250 bp, confirmed by gel electrophoresis, and fragments were labeled at the 3'-ends with biotin-ddUTP (Roche Diagnostics, Indianapolis, IN) using Terminal deoxynucleotidyl transferase (Roche).

    Article Title: ?-Lactam Resistance Response Triggered by Inactivation of a Nonessential Penicillin-Binding Protein
    Article Snippet: Cell lysates were treated with RNase I (Qiagen) to prevent accidental carryover of RNA to the microarray. .. Genomic DNA was partially digested with DNase I (Amersham Biosciences, Piscataway, NJ) to a fragment size of ∼50–250 bp, confirmed by gel electrophoresis, and fragments were labeled at the 3′-ends with biotin-ddUTP (Roche Diagnostics, Indianapolis, IN) using Terminal deoxynucleotidyl transferase (Roche).

    Purification:

    Article Title: Cells adapt to the epigenomic disruption caused by histone deacetylase inhibitors through a coordinated, chromatin-mediated transcriptional response
    Article Snippet: .. Double stranded cDNA was synthesised using the cDNA Synthesis System, including RNase I and Proteinase K treatment followed by DNA clean up using the PCR purification kit (Qiagen). .. Samples were labelled with cy3 using a Nimblegen One-Colour Labeling Kit, mixed with alignment oligos and sample tracking control oligos (Nimblegen Hybridisation and Sample Tracking Control Kits) and hybridised to a 12 × 135k HD2 expression array (Roche Nimblegen, containing 3 probes per sequence for 44,049 human sequences) and scanned on a Nimblegen MS200 Microarray scanner.

    Article Title: Acute myeloid leukemia requires Hhex to enable PRC2-mediated epigenetic repression of Cdkn2a
    Article Snippet: .. Following reversal of cross-links and RNase I and proteinase K treatments, extracted DNA was purified using the QIAquick purification kit (Qiagen). .. For RNA sequencing, see the Supplemental Material.

    Article Title: Scd6 targets eIF4G to repress translation: RGG-motif proteins as a class of eIF4G-binding proteins
    Article Snippet: Proteins were purified from E. coli according to standard protocols using glutathione sepharose beads (GE), Ni-NTA agarose (Qiagen) or FLAG-agarose (Sigma-Aldrich) according to standard protocols. .. To remove RNA that might provide bridging interactions, extracts after breaking open the cells during the time of protein purification were treated for 20 min with Qiagen RNase A (1mg/ml).

    Article Title: The barrier-to-autointegration protein is a host factor for HIV type 1 integration
    Article Snippet: Human HMG I(Y) was purified from Escherichia coli cells as described ( ) after expression from plasmid pET7C ( ). .. For functional reconstitution of salt-stripped PICs, Qiagen RNase A was used.

    Article Title: Epigenetic Regulation of Wnt Pathway Antagonists in Human Glioblastoma Multiforme
    Article Snippet: Nuclei isolated from TSA-treated cells (1 μM for 24 h) or control-treated (DMSO) T98 cells were suspended in 100 μL of digestion buffer (50 mM Tris [pH 8.0], 100 mM NaCl, 3 mM MgCl2 , 1 mM CaCl2 , 0.15 mM Spermine, and 0.5 mM spermidine) and 20 μg of RNAse I (Qiagen). .. DNA was purified with phenol/chloroform extraction and ethanol precipitated.

    Protein Purification:

    Article Title: Scd6 targets eIF4G to repress translation: RGG-motif proteins as a class of eIF4G-binding proteins
    Article Snippet: .. To remove RNA that might provide bridging interactions, extracts after breaking open the cells during the time of protein purification were treated for 20 min with Qiagen RNase A (1mg/ml). .. Purified protein was concentrated and dialyzed into 20 mM Tris, 100 mM NaCl and 10% glycerol.

    Sequencing:

    Article Title: Cells adapt to the epigenomic disruption caused by histone deacetylase inhibitors through a coordinated, chromatin-mediated transcriptional response
    Article Snippet: Double stranded cDNA was synthesised using the cDNA Synthesis System, including RNase I and Proteinase K treatment followed by DNA clean up using the PCR purification kit (Qiagen). .. Samples were labelled with cy3 using a Nimblegen One-Colour Labeling Kit, mixed with alignment oligos and sample tracking control oligos (Nimblegen Hybridisation and Sample Tracking Control Kits) and hybridised to a 12 × 135k HD2 expression array (Roche Nimblegen, containing 3 probes per sequence for 44,049 human sequences) and scanned on a Nimblegen MS200 Microarray scanner.

    Article Title: Facile single-stranded DNA sequencing of human plasma DNA via thermostable group II intron reverse transcriptase template switching
    Article Snippet: Preparation of human plasma DNA cfDNA was isolated from plasma of a healthy male individual obtained from the Genome Sequencing and Analysis Facility at the University of Texas at Austin. .. To extract DNA, 400 μl of plasma was incubated with RNase I (Qiagen; 800 μg) as suggested by the manufacturer’s protocol and then processed by using a Qiagen DSP DNA Blood Mini Kit (Qiagen).

    Article Title: Mouse APOBEC3 Restricts Friend Leukemia Virus Infection and Pathogenesis In Vivo ▿
    Article Snippet: Paragraph title: Sequence analysis of proviral DNA. ... F-MuLV in 1 ml of culture medium were inoculated onto a culture of Mus dunni cells and incubated for 18 h. After trypsinization and washing, the cells were treated with RNase I, and their DNA was isolated by using DNeasy (Qiagen, Hilden, Germany).

    Article Title: Detection and genetic characterization of Seoul Virus from commensal brown rats in France
    Article Snippet: Paragraph title: Viral enrichment and Illumina Hiseq 2500 v3 sequencing ... Condition S3 combined a step-wise filtration and a 2-step digestion with 25U of RNase I at 37°C for 90 min in 1× RNase I buffer and DNA is removed on-column (Qiagen).

    Article Title: Patterns of homoeologous gene expression shown by RNA sequencing in hexaploid bread wheat
    Article Snippet: .. Experimental validation of homoeologue sequence variants (HSVs) Two micrograms of total RNA extracted from euploid (CS) and nullitetra wheat root samples using the TRIzol Reagent (Invitrogen) was reverse-transcribed to synthesize first-strand cDNA using the SuperScript II First-Strand Synthesis System for RT-PCR Kit (Invitrogen). cDNA was treated with Qiagen RNase A to remove any residual RNA. .. We selected 10 genes with differential expression of homoeoloci and with three homoeolocus-specific tentative contigs (TCs) identified from a BLAST search of the T. aestivum TIGR database (Additional file : Table S4A).

    Article Title: Genome resequencing and transcriptome profiling reveal structural diversity and expression patterns of constitutive disease resistance genes in Huanglongbing-tolerant Poncirus trifoliata and its hybrids
    Article Snippet: Paragraph title: Plant material, DNA and RNA isolation and HiSeq sequencing ... Genomic DNA was extracted from the leaves using the CTAB method and treated with RNase I (Qiagen, Hilden, Germany).

    Chromatin Immunoprecipitation:

    Article Title: Acute myeloid leukemia requires Hhex to enable PRC2-mediated epigenetic repression of Cdkn2a
    Article Snippet: Paragraph title: ChIP ... Following reversal of cross-links and RNase I and proteinase K treatments, extracted DNA was purified using the QIAquick purification kit (Qiagen).

    RNA Extraction:

    Article Title: Detection and genetic characterization of Seoul Virus from commensal brown rats in France
    Article Snippet: The resulting supernatant was transferred to a new tube and 2 different treatments were applied: for the condition S2 a step-wise filtration process involving 0.45 μm polyethersulfone membrane filters (diameter 13 mm) (Millipore) was performed before RNA extraction. .. Condition S3 combined a step-wise filtration and a 2-step digestion with 25U of RNase I at 37°C for 90 min in 1× RNase I buffer and DNA is removed on-column (Qiagen).

    Plasmid Preparation:

    Article Title: The barrier-to-autointegration protein is a host factor for HIV type 1 integration
    Article Snippet: Human HMG I(Y) was purified from Escherichia coli cells as described ( ) after expression from plasmid pET7C ( ). .. For functional reconstitution of salt-stripped PICs, Qiagen RNase A was used.

    Article Title: Mouse APOBEC3 Restricts Friend Leukemia Virus Infection and Pathogenesis In Vivo ▿
    Article Snippet: F-MuLV in 1 ml of culture medium were inoculated onto a culture of Mus dunni cells and incubated for 18 h. After trypsinization and washing, the cells were treated with RNase I, and their DNA was isolated by using DNeasy (Qiagen, Hilden, Germany). .. The amplified fragments were cloned into the pCR-Blunt vector using a Zero Blunt TOPO PCR cloning kit (Invitrogen).

    Software:

    Article Title: PTGER3 induces ovary tumorigenesis and confers resistance to cisplatin therapy through up-regulation Ras-MAPK/Erk-ETS1-ELK1/CFTR1 axis
    Article Snippet: Apoptosis was analyzed by a FACSCalibur flow cytometer and CellQuest Pro software according to manufacturer's recommended protocol. .. The cells then were washed with cold phosphate-buffered saline (PBS), treated with 100 μg of RNase A (Qiagen), and stained with 50 μg of propidium iodide.

    Negative Control:

    Article Title: PTGER3 induces ovary tumorigenesis and confers resistance to cisplatin therapy through up-regulation Ras-MAPK/Erk-ETS1-ELK1/CFTR1 axis
    Article Snippet: 2.7 Apoptosis and cell cycle analysis The resistant A2780-CP20 and OVCAR5 cell lines were transfected with non-silencing negative control or PTGER3 siRNA for 72 h and stained with the FITC-Apoptosis Detection Kit (BD Pharmingen) and propidium iodide (Molecular Probes). .. The cells then were washed with cold phosphate-buffered saline (PBS), treated with 100 μg of RNase A (Qiagen), and stained with 50 μg of propidium iodide.

    Recombinant:

    Article Title: The barrier-to-autointegration protein is a host factor for HIV type 1 integration
    Article Snippet: Purified recombinant human BAF was a generous gift from Robert Craigie, National Institute of Diabetes and Digestive and Kidney Diseases. .. For functional reconstitution of salt-stripped PICs, Qiagen RNase A was used.

    Sample Prep:

    Article Title: Genome resequencing and transcriptome profiling reveal structural diversity and expression patterns of constitutive disease resistance genes in Huanglongbing-tolerant Poncirus trifoliata and its hybrids
    Article Snippet: Genomic DNA was extracted from the leaves using the CTAB method and treated with RNase I (Qiagen, Hilden, Germany). .. RNA samples were shipped to Novogene Corporation where mRNA were enriched and converted into cDNAs, which were sequenced on an Illumina HiSeq 2500 using the Illumina ‘TruSeq RNA-Seq Sample Prep kit.’

    Functional Assay:

    Article Title: Cells adapt to the epigenomic disruption caused by histone deacetylase inhibitors through a coordinated, chromatin-mediated transcriptional response
    Article Snippet: Double stranded cDNA was synthesised using the cDNA Synthesis System, including RNase I and Proteinase K treatment followed by DNA clean up using the PCR purification kit (Qiagen). .. Genes with a P value smaller than 0.05 and FC larger than 1.5 have been selected for further analysis including functional annotation and network construction.

    Article Title: The barrier-to-autointegration protein is a host factor for HIV type 1 integration
    Article Snippet: .. For functional reconstitution of salt-stripped PICs, Qiagen RNase A was used. .. Single-stranded DNA-binding protein was from Stratagene, BSA was from New England Biolabs, and digitonin, aprotinin, and Nycodenz were from Sigma.

    Concentration Assay:

    Article Title: Detection and genetic characterization of Seoul Virus from commensal brown rats in France
    Article Snippet: Condition S3 combined a step-wise filtration and a 2-step digestion with 25U of RNase I at 37°C for 90 min in 1× RNase I buffer and DNA is removed on-column (Qiagen). .. Total RNA concentration was quantified with Quant-iT ribogreen RNA kit (Invitrogen).

    Staining:

    Article Title: Evaluation of a microarray-hybridization based method applicable for discovery of single nucleotide polymorphisms (SNPs) in the Pseudomonas aeruginosa genome
    Article Snippet: Cell lysates were treated with RNase I (Qiagen) to prevent accidental carryover of RNA to the microarray. .. After hybridization the GeneChips were washed, stained with SA-PE and read using an Affymetrix GeneChip fluidic station and scanner according to Affymetrix standard protocols (Affymetrix, Santa Clara, CA).

    Article Title: PTGER3 induces ovary tumorigenesis and confers resistance to cisplatin therapy through up-regulation Ras-MAPK/Erk-ETS1-ELK1/CFTR1 axis
    Article Snippet: .. The cells then were washed with cold phosphate-buffered saline (PBS), treated with 100 μg of RNase A (Qiagen), and stained with 50 μg of propidium iodide. .. Cell cycle profiles were analyzed by using the FACSCalibur flow cytometer.

    Article Title: ?-Lactam Resistance Response Triggered by Inactivation of a Nonessential Penicillin-Binding Protein
    Article Snippet: Cell lysates were treated with RNase I (Qiagen) to prevent accidental carryover of RNA to the microarray. .. After hybridization the microarrays were washed, stained with SA-PE and read using an Affymetrix GeneChip fluidic station and scanner according to Affymetrix standard protocols (Affymetrix, Santa Clara, CA).

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    Qiagen qiagen rnase a
    Qiagen Rnase A, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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