18427 013 klenow dna polymerase t4 pnk minelute reaction cleanup kit  (Thermo Fisher)


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    Name:
    dNTP Mix
    Description:
    This dNTP mix consists of four nucleotides dATP dCTP dGTP dTTP 2 deoxynucleoside 5 triphosphates each at a concentration of 10 mM in a solution of 0 6 mM Tris HCl pH 7 5 This mix is suitable for use in PCR sequencing fill in nick translation cDNA synthesis TdT tailing reactions and dilution of radiolabeled dNTPs
    Catalog Number:
    18427013
    Price:
    None
    Applications:
    PCR|PCR & Real-Time PCR|PCR Buffers, dNTPs, MgCl2 & General Reagents
    Category:
    Oligos Primers Probes Nucleotides
    Buy from Supplier


    Structured Review

    Thermo Fisher 18427 013 klenow dna polymerase t4 pnk minelute reaction cleanup kit
    This dNTP mix consists of four nucleotides dATP dCTP dGTP dTTP 2 deoxynucleoside 5 triphosphates each at a concentration of 10 mM in a solution of 0 6 mM Tris HCl pH 7 5 This mix is suitable for use in PCR sequencing fill in nick translation cDNA synthesis TdT tailing reactions and dilution of radiolabeled dNTPs
    https://www.bioz.com/result/18427 013 klenow dna polymerase t4 pnk minelute reaction cleanup kit/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    18427 013 klenow dna polymerase t4 pnk minelute reaction cleanup kit - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    Transduction:

    Article Title: Vpx mediated degradation of SAMHD1 has only a very limited effect on lentiviral transduction rate in ex vivo cultured HSPCs
    Article Snippet: .. THP-1 cells transduced with lentiviral vectors encoding SAMHD1 shRNA were selected by culturing in a medium containing 0.8 μg/ml puromycin. dNs consisted of a mixture of dA (D8668), dC (D0776), dG (D0901) and dT (T1895; all from Sigma-Aldrich). dNTPs (R0192) were purchased from Fermentas (Glen Burnie, MD). .. Cytokine cocktail for HSPC transduction consisted of a mixture of stem cell factor (SCF) at 100 ng/ml, Fms-related tyrosine kinase 3 ligand (Flt3L) at 100 ng/ml, interleukin-3 (IL-3) at 60 ng/ml and thrombopoietin (TPO) at 10 ng/ml (Immunotools, Friesoythe, Germany).

    Amplification:

    Article Title: Evolution of blue-flowered species of genus Linum based on high-throughput sequencing of ribosomal RNA genes
    Article Snippet: .. Amplification was carried out in 25 μl of PCR mix containing 1× KAPA2G buffer A (Kapa Biosystems), 250 μM each dNTP (Thermo Scientific, USA), 0.12 μM of MIDх-454 forward and reverse primers, 0.5 U of KAPA2G Fast HotStart DNA Polymerase (Kapa Biosystems), and 3 μl of the diluted (1:20) first-stage PCR product. .. The PCR products were separated in 1.5% agarose gel for 5 h at 45 V using ТВЕ buffer and then stained with ethidium bromide.

    Article Title: Reconstituting ParA/ParB-mediated transport of DNA cargo
    Article Snippet: .. pBR322:: sopC plasmid (PCR template) PCR Primers for sopC DNA amplification: (Primers synthesized by IDT) 10 mM dNTPs (Life Technologies; Cat. # 18427–013) 100 mM MgSO4 (New England Biolabs, Cat. # M0254S) 2000 U/mL VENT Polymerase (New England Biolabs, Cat. # M0254S) SYBR Gold DNA Stain (Life Technologies, Cat. # S-11494) Illustra MicroSpin S-400HR columns (GE Healthcare, Cat # 27–5140-01) MyOne Streptavidin C1 Dynabeads (Life Technologies, Cat. # 65001) 20 U/μL PstI Restriction Enzyme (New England Biolabs, Cat. # R0140S) Phenol:CHCl3:Isoamyl Alcohol (UltraPure 25:24:1 v/v) Ethanol. .. PCR Thermocycler Tabletop centrifuge Tube rotator BioMag Separator (Advanced Magnetics Inc.) Nanodrop Spectrophotometer.

    Article Title: Significance of UGT1A1*28 Genotype in Patients with Advanced Liver Injury Caused By Chronic Hepatitis C
    Article Snippet: .. The amplification reaction was performed in a total volume of 25 μL, and the reaction mix contained 20 pmol of each primer, 50–100 ng of genomic DNA, 200 mmol/L of each dNTP (Fermentas, ON, Canada), 1× PCR reaction buffer (Qiagen), 1× Q solution (Qiagen), 2.75 mmol/L MgCl2 , 1 U HotStar DNA polymerase (Qiagen). .. The temperature profile of the PCR reactions was for the initial activation of DNA polymerase set at 95 °C for 15 min, followed by 35 cycles of 30 sec denaturation at 95 °C, 30 sec annealing at 63 °C, and 30 sec elongation at 72 °C, ended by a final extension period of 7 min at 72 °C.

    Synthesized:

    Article Title: Correlation between CXCR4/CXCR7/CXCL12 chemokine axis expression and prognosis in lymph‐node‐positive lung cancer patients, et al. Correlation between CXCR4/CXCR7/CXCL12 chemokine axis expression and prognosis in lymph‐node‐positive lung cancer patients
    Article Snippet: .. RNA was converted into cDNA using the Prime Script RT Master Mix (Takara Bio, Shiga, Japan) according to the manufacturer's protocol. cDNA was synthesized using 500 ng of each mRNA sample with Oligo‐dT‐Primers (Life Technologies, Darmstadt, Germany), Superscript II Reverse Transcriptase (Life Technologies) and dNTP (Fermentas, Waltham, MA, USA) following the manufacturer's instructions. .. The cDNA was diluted fivefold and used for real‐time PCR analysis with the Thermal Cycler Dice Real Time System II TP‐900 (Takara Bio) using a TaqMan Gene Expression Assay probe and primer mix (Applied Biosystems, Foster City, CA, USA) according to the manufacturer's instructions.

    Article Title: Reconstituting ParA/ParB-mediated transport of DNA cargo
    Article Snippet: .. pBR322:: sopC plasmid (PCR template) PCR Primers for sopC DNA amplification: (Primers synthesized by IDT) 10 mM dNTPs (Life Technologies; Cat. # 18427–013) 100 mM MgSO4 (New England Biolabs, Cat. # M0254S) 2000 U/mL VENT Polymerase (New England Biolabs, Cat. # M0254S) SYBR Gold DNA Stain (Life Technologies, Cat. # S-11494) Illustra MicroSpin S-400HR columns (GE Healthcare, Cat # 27–5140-01) MyOne Streptavidin C1 Dynabeads (Life Technologies, Cat. # 65001) 20 U/μL PstI Restriction Enzyme (New England Biolabs, Cat. # R0140S) Phenol:CHCl3:Isoamyl Alcohol (UltraPure 25:24:1 v/v) Ethanol. .. PCR Thermocycler Tabletop centrifuge Tube rotator BioMag Separator (Advanced Magnetics Inc.) Nanodrop Spectrophotometer.

    shRNA:

    Article Title: Vpx mediated degradation of SAMHD1 has only a very limited effect on lentiviral transduction rate in ex vivo cultured HSPCs
    Article Snippet: .. THP-1 cells transduced with lentiviral vectors encoding SAMHD1 shRNA were selected by culturing in a medium containing 0.8 μg/ml puromycin. dNs consisted of a mixture of dA (D8668), dC (D0776), dG (D0901) and dT (T1895; all from Sigma-Aldrich). dNTPs (R0192) were purchased from Fermentas (Glen Burnie, MD). .. Cytokine cocktail for HSPC transduction consisted of a mixture of stem cell factor (SCF) at 100 ng/ml, Fms-related tyrosine kinase 3 ligand (Flt3L) at 100 ng/ml, interleukin-3 (IL-3) at 60 ng/ml and thrombopoietin (TPO) at 10 ng/ml (Immunotools, Friesoythe, Germany).

    Polymerase Chain Reaction:

    Article Title: PCR detection of Burkholderia multivorans in water and soil samples
    Article Snippet: .. The PCR mix contained 1× CorelLoad PCR buffer (Qiagen), 0.25 mM dNTP (Applied Biosystems), 1 U Taq (Qiagen), 0.5 μM of both primers, 1× Q-solution (Qiagen) and 200 ng/μl BSA (Roche). .. For each sample, 2 μl DNA was added to 23 μl PCR master mix.

    Article Title: UV-B induced transcript accumulation of DAHP synthase in suspension-cultured Catharanthus roseus cells
    Article Snippet: .. A common PCR mix for CrRPS9 and CrDHS1 for each treatment was prepared containing one μl of the RT reaction mix per 20 μl reaction volume containing 0.4 U of Taq DNA polymerase (Fermentas), 0.1 mM dNTP (Fermentas) and 200 μM of each dNTP and later split into equal half to add 100 pM of gene-specific primer in a 1X reaction buffer. .. Reactions were amplified for a total of 15 cycles in the Minicycler (MJ Research PTC-150) using 94°C denaturation (1 min), 52°C annealing for CrDHS1 and 50°C for CrRPS9 (1 min) and 72°C extension (1 min), followed by a further 5 min extension.

    Article Title: A mutant Pfu DNA polymerase designed for advanced uracil-excision DNA engineering
    Article Snippet: .. PCR with dUTP replacing dTTP was performed using standard Phusion (Finnzymes) reaction conditions as recommended by the manufacturer, using the dUTP/dNTPs mix (Fermentas) instead of dNTPs. .. The pET-Pfu-vector was a kind gift from Jens Lykke-Andersen.

    Article Title: Evolution of blue-flowered species of genus Linum based on high-throughput sequencing of ribosomal RNA genes
    Article Snippet: .. Amplification was carried out in 25 μl of PCR mix containing 1× KAPA2G buffer A (Kapa Biosystems), 250 μM each dNTP (Thermo Scientific, USA), 0.12 μM of MIDх-454 forward and reverse primers, 0.5 U of KAPA2G Fast HotStart DNA Polymerase (Kapa Biosystems), and 3 μl of the diluted (1:20) first-stage PCR product. .. The PCR products were separated in 1.5% agarose gel for 5 h at 45 V using ТВЕ buffer and then stained with ethidium bromide.

    Article Title: Reconstituting ParA/ParB-mediated transport of DNA cargo
    Article Snippet: .. pBR322:: sopC plasmid (PCR template) PCR Primers for sopC DNA amplification: (Primers synthesized by IDT) 10 mM dNTPs (Life Technologies; Cat. # 18427–013) 100 mM MgSO4 (New England Biolabs, Cat. # M0254S) 2000 U/mL VENT Polymerase (New England Biolabs, Cat. # M0254S) SYBR Gold DNA Stain (Life Technologies, Cat. # S-11494) Illustra MicroSpin S-400HR columns (GE Healthcare, Cat # 27–5140-01) MyOne Streptavidin C1 Dynabeads (Life Technologies, Cat. # 65001) 20 U/μL PstI Restriction Enzyme (New England Biolabs, Cat. # R0140S) Phenol:CHCl3:Isoamyl Alcohol (UltraPure 25:24:1 v/v) Ethanol. .. PCR Thermocycler Tabletop centrifuge Tube rotator BioMag Separator (Advanced Magnetics Inc.) Nanodrop Spectrophotometer.

    Article Title: Significance of UGT1A1*28 Genotype in Patients with Advanced Liver Injury Caused By Chronic Hepatitis C
    Article Snippet: .. The amplification reaction was performed in a total volume of 25 μL, and the reaction mix contained 20 pmol of each primer, 50–100 ng of genomic DNA, 200 mmol/L of each dNTP (Fermentas, ON, Canada), 1× PCR reaction buffer (Qiagen), 1× Q solution (Qiagen), 2.75 mmol/L MgCl2 , 1 U HotStar DNA polymerase (Qiagen). .. The temperature profile of the PCR reactions was for the initial activation of DNA polymerase set at 95 °C for 15 min, followed by 35 cycles of 30 sec denaturation at 95 °C, 30 sec annealing at 63 °C, and 30 sec elongation at 72 °C, ended by a final extension period of 7 min at 72 °C.

    Staining:

    Article Title: Reconstituting ParA/ParB-mediated transport of DNA cargo
    Article Snippet: .. pBR322:: sopC plasmid (PCR template) PCR Primers for sopC DNA amplification: (Primers synthesized by IDT) 10 mM dNTPs (Life Technologies; Cat. # 18427–013) 100 mM MgSO4 (New England Biolabs, Cat. # M0254S) 2000 U/mL VENT Polymerase (New England Biolabs, Cat. # M0254S) SYBR Gold DNA Stain (Life Technologies, Cat. # S-11494) Illustra MicroSpin S-400HR columns (GE Healthcare, Cat # 27–5140-01) MyOne Streptavidin C1 Dynabeads (Life Technologies, Cat. # 65001) 20 U/μL PstI Restriction Enzyme (New England Biolabs, Cat. # R0140S) Phenol:CHCl3:Isoamyl Alcohol (UltraPure 25:24:1 v/v) Ethanol. .. PCR Thermocycler Tabletop centrifuge Tube rotator BioMag Separator (Advanced Magnetics Inc.) Nanodrop Spectrophotometer.

    Plasmid Preparation:

    Article Title: Reconstituting ParA/ParB-mediated transport of DNA cargo
    Article Snippet: .. pBR322:: sopC plasmid (PCR template) PCR Primers for sopC DNA amplification: (Primers synthesized by IDT) 10 mM dNTPs (Life Technologies; Cat. # 18427–013) 100 mM MgSO4 (New England Biolabs, Cat. # M0254S) 2000 U/mL VENT Polymerase (New England Biolabs, Cat. # M0254S) SYBR Gold DNA Stain (Life Technologies, Cat. # S-11494) Illustra MicroSpin S-400HR columns (GE Healthcare, Cat # 27–5140-01) MyOne Streptavidin C1 Dynabeads (Life Technologies, Cat. # 65001) 20 U/μL PstI Restriction Enzyme (New England Biolabs, Cat. # R0140S) Phenol:CHCl3:Isoamyl Alcohol (UltraPure 25:24:1 v/v) Ethanol. .. PCR Thermocycler Tabletop centrifuge Tube rotator BioMag Separator (Advanced Magnetics Inc.) Nanodrop Spectrophotometer.