16 doxylstearic acids  (Millipore)


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  • 99
    Name:
    Amino Acids
    Description:

    Catalog Number:
    paa20
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    Structured Review

    Millipore 16 doxylstearic acids

    https://www.bioz.com/result/16 doxylstearic acids/product/Millipore
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    16 doxylstearic acids - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Modular Self-Assembly of Protein Cage Lattices for Multistep Catalysis
    Article Snippet: .. A truncated form of P22 Scaffolding Protein (amino acids 142 to 303, hereafter referred to as SP141 ) and P22 Coat Protein (CP) were cloned into the first and the second multi cloning sites of the pRSFDuet-1 vector (Novagen), respectively. .. Two repeats of a net negatively charged peptide with sequence VAALEKE (E2 peptide) was introduced at the C-terminus of the CP (hereafter referred to as CP-E2).

    Amplification:

    Article Title: Phosphoinositides are essential co-activators for p21-activated kinase 1 (Pak1)
    Article Snippet: .. The Pak1 autoregulatory domain (amino acids 1–84) was amplified by PCR as a BamHI/SalI or BglII/EcoRI fragment and subcloned into pGEX-KG-KAN (Novagen) or pEGFP-C1 (Clontech), respectively. .. For cloning of GST-Paktide, oligonucleotides encoding peptide sequence GRRRRRSWYWDG were annealed and ligated into pGEX-6P-1 (GE Healthcare) using BamHI/EcoRI .

    Scaffolding:

    Article Title: Modular Self-Assembly of Protein Cage Lattices for Multistep Catalysis
    Article Snippet: .. A truncated form of P22 Scaffolding Protein (amino acids 142 to 303, hereafter referred to as SP141 ) and P22 Coat Protein (CP) were cloned into the first and the second multi cloning sites of the pRSFDuet-1 vector (Novagen), respectively. .. Two repeats of a net negatively charged peptide with sequence VAALEKE (E2 peptide) was introduced at the C-terminus of the CP (hereafter referred to as CP-E2).

    Mutagenesis:

    Article Title: Diindolylmethane Analogs Bind NR4A1 and Are NR4A1 Antagonists in Colon Cancer Cells
    Article Snippet: .. The Flag-tagged full-length FLAG-NR4A1 and mutant FLAG-NR4A1(A–D) and FLAG-NR4A1(C–F) expression plasmids were constructed by inserting PCR-amplified full-length NR4A1 (amino acids 1–599) into the Eco RI/ Bam HI site and C-terminal NR4A1 (amino acids 67–599) and N-terminal NR4A1 (amino acids 1–354) into the Eco RI/ Kpm I site of p3XFLAG-CMV-10 expression vector (Sigma-Aldrich). ..

    Construct:

    Article Title: Diindolylmethane Analogs Bind NR4A1 and Are NR4A1 Antagonists in Colon Cancer Cells
    Article Snippet: .. The Flag-tagged full-length FLAG-NR4A1 and mutant FLAG-NR4A1(A–D) and FLAG-NR4A1(C–F) expression plasmids were constructed by inserting PCR-amplified full-length NR4A1 (amino acids 1–599) into the Eco RI/ Bam HI site and C-terminal NR4A1 (amino acids 67–599) and N-terminal NR4A1 (amino acids 1–354) into the Eco RI/ Kpm I site of p3XFLAG-CMV-10 expression vector (Sigma-Aldrich). ..

    Purification:

    Article Title: LAPTM5 Protein Is a Positive Regulator of Proinflammatory Signaling Pathways in Macrophages *
    Article Snippet: .. For pulldown of endogenous A20, GST fusion proteins were produced in bacteria and purified on glutathione-agarose beads (Sigma). .. 50 μg of GST or GST C terminus (LAPTM5) were incubated with 2 mg of RAW264.7 cell lysate for 4 h at 4 °C.

    Produced:

    Article Title: LAPTM5 Protein Is a Positive Regulator of Proinflammatory Signaling Pathways in Macrophages *
    Article Snippet: .. For pulldown of endogenous A20, GST fusion proteins were produced in bacteria and purified on glutathione-agarose beads (Sigma). .. 50 μg of GST or GST C terminus (LAPTM5) were incubated with 2 mg of RAW264.7 cell lysate for 4 h at 4 °C.

    other:

    Article Title: MUC1 is a receptor for the Salmonella SiiE adhesin that enables apical invasion into enterocytes
    Article Snippet: Enzymatic removal of sialic acids Desialylation of HT29-MTX and ΔMUC1 cells was achieved by incubating cells grown in a 24-well plate with 250 mU/mL neuraminidase (Sigma) in DPBS for 2 h at 37°C.

    Expressing:

    Article Title: Diindolylmethane Analogs Bind NR4A1 and Are NR4A1 Antagonists in Colon Cancer Cells
    Article Snippet: .. The Flag-tagged full-length FLAG-NR4A1 and mutant FLAG-NR4A1(A–D) and FLAG-NR4A1(C–F) expression plasmids were constructed by inserting PCR-amplified full-length NR4A1 (amino acids 1–599) into the Eco RI/ Bam HI site and C-terminal NR4A1 (amino acids 67–599) and N-terminal NR4A1 (amino acids 1–354) into the Eco RI/ Kpm I site of p3XFLAG-CMV-10 expression vector (Sigma-Aldrich). ..

    Polymerase Chain Reaction:

    Article Title: Phosphoinositides are essential co-activators for p21-activated kinase 1 (Pak1)
    Article Snippet: .. The Pak1 autoregulatory domain (amino acids 1–84) was amplified by PCR as a BamHI/SalI or BglII/EcoRI fragment and subcloned into pGEX-KG-KAN (Novagen) or pEGFP-C1 (Clontech), respectively. .. For cloning of GST-Paktide, oligonucleotides encoding peptide sequence GRRRRRSWYWDG were annealed and ligated into pGEX-6P-1 (GE Healthcare) using BamHI/EcoRI .

    Article Title: Diindolylmethane Analogs Bind NR4A1 and Are NR4A1 Antagonists in Colon Cancer Cells
    Article Snippet: .. The Flag-tagged full-length FLAG-NR4A1 and mutant FLAG-NR4A1(A–D) and FLAG-NR4A1(C–F) expression plasmids were constructed by inserting PCR-amplified full-length NR4A1 (amino acids 1–599) into the Eco RI/ Bam HI site and C-terminal NR4A1 (amino acids 67–599) and N-terminal NR4A1 (amino acids 1–354) into the Eco RI/ Kpm I site of p3XFLAG-CMV-10 expression vector (Sigma-Aldrich). ..

    Affinity Purification:

    Article Title: Loss of the tumor suppressor BAP1 causes myeloid transformation
    Article Snippet: .. Flag-tagged human BAP1 (amino acids 2–729) was co-expressed with His-tagged human ASXL1 (amino acids 2–365) in Sf9 cells and affinity purified through anti-Flag M2 affinity resin (Sigma) and a Superdex S200 size-exclusion column (GE) in 300 mM NaCl, 50 mM Tris 8.0, and 2 mM β-mercaptoethanol (Buffer A). .. The BAP1/ASXL1 concentration was calculated by measuring the absorbance at 280 nm, using an extinction coefficient 78,000 M−1 cm−1 .

    Plasmid Preparation:

    Article Title: Modular Self-Assembly of Protein Cage Lattices for Multistep Catalysis
    Article Snippet: .. A truncated form of P22 Scaffolding Protein (amino acids 142 to 303, hereafter referred to as SP141 ) and P22 Coat Protein (CP) were cloned into the first and the second multi cloning sites of the pRSFDuet-1 vector (Novagen), respectively. .. Two repeats of a net negatively charged peptide with sequence VAALEKE (E2 peptide) was introduced at the C-terminus of the CP (hereafter referred to as CP-E2).

    Article Title: Diindolylmethane Analogs Bind NR4A1 and Are NR4A1 Antagonists in Colon Cancer Cells
    Article Snippet: .. The Flag-tagged full-length FLAG-NR4A1 and mutant FLAG-NR4A1(A–D) and FLAG-NR4A1(C–F) expression plasmids were constructed by inserting PCR-amplified full-length NR4A1 (amino acids 1–599) into the Eco RI/ Bam HI site and C-terminal NR4A1 (amino acids 67–599) and N-terminal NR4A1 (amino acids 1–354) into the Eco RI/ Kpm I site of p3XFLAG-CMV-10 expression vector (Sigma-Aldrich). ..