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GE Healthcare 16 10 hi prep heparin ff column
16 10 Hi Prep Heparin Ff Column, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 87/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/16 10 hi prep heparin ff column/product/GE Healthcare
Average 87 stars, based on 3 article reviews
Price from $9.99 to $1999.99
16 10 hi prep heparin ff column - by Bioz Stars, 2020-04
87/100 stars

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Clone Assay:

Article Title: Molecular breeding of polymerases for resistance to environmental inhibitors
Article Snippet: .. Promising polymerase clones were expressed and purified as described ( ) using a 16/10 Hi-Prep Heparin FF Column (Amersham Pharmacia Biotech). ..

Article Title: Molecular breeding of polymerases for amplification of ancient DNA
Article Snippet: The product was gel-purified and reamplified with primers 7,8 and cloned XbaI / SalI into pASK75 to create library 3T (1 × 109 cfu, 70% active clones). .. Expression of polymerases for characterization and ancient DNA PCR was as described , but using a 16/10 Hi-Prep Heparin FF Column (Amersham Pharmacia Biotech) column to purify heat-cleared (50°C, 30min) Bugbuster (Novagen) lysate with Complete™ EDTA-free protease inhibitor cocktail (Roche).

Article Title: Evolving a polymerase for hydrophobic base analogues
Article Snippet: Clones analyzed in more detail in this report derive from selection rounds 4 and 5. .. Expression of polymerases for characterization was as described using a 16/10 Hi-Prep Heparin FF Column (Amersham Pharmacia Biotech).

In Vivo:

Article Title: Evolving a polymerase for hydrophobic base analogues
Article Snippet: Expression of polymerases for characterization was as described using a 16/10 Hi-Prep Heparin FF Column (Amersham Pharmacia Biotech). .. Mutation rates were determined using a well-established in vivo gap filling assay (M. Arana, PH, T. Kunkel, unpublished results).

Amplification:

Article Title: Molecular breeding of polymerases for amplification of ancient DNA
Article Snippet: In StEP, genes to be recombined PCR are amplified with common flanking primers but extension times that are too short to allow complete primer extension during each cycle of PCR. .. Expression of polymerases for characterization and ancient DNA PCR was as described , but using a 16/10 Hi-Prep Heparin FF Column (Amersham Pharmacia Biotech) column to purify heat-cleared (50°C, 30min) Bugbuster (Novagen) lysate with Complete™ EDTA-free protease inhibitor cocktail (Roche).

Ancient DNA Assay:

Article Title: Molecular breeding of polymerases for amplification of ancient DNA
Article Snippet: .. Expression of polymerases for characterization and ancient DNA PCR was as described , but using a 16/10 Hi-Prep Heparin FF Column (Amersham Pharmacia Biotech) column to purify heat-cleared (50°C, 30min) Bugbuster (Novagen) lysate with Complete™ EDTA-free protease inhibitor cocktail (Roche). ..

Mutagenesis:

Article Title: A polymerase engineered for bisulfite sequencing
Article Snippet: TgoT comprises a A485L mutation of the Tgo DNA polymerase. .. Expression of polymerases for characterization was as described ( , ) using a 16/10 Hi-Prep Heparin FF Column (Amersham Pharmacia Biotech).

Article Title: Evolving a polymerase for hydrophobic base analogues
Article Snippet: Selected mutations were reverted back to wild-type Taq sequence using Quickchange Mutagenesis using Pfu Turbo (Stratagene) and primers 31–40. .. Expression of polymerases for characterization was as described using a 16/10 Hi-Prep Heparin FF Column (Amersham Pharmacia Biotech).

Protease Inhibitor:

Article Title: Molecular breeding of polymerases for amplification of ancient DNA
Article Snippet: .. Expression of polymerases for characterization and ancient DNA PCR was as described , but using a 16/10 Hi-Prep Heparin FF Column (Amersham Pharmacia Biotech) column to purify heat-cleared (50°C, 30min) Bugbuster (Novagen) lysate with Complete™ EDTA-free protease inhibitor cocktail (Roche). ..

Polymerase Chain Reaction:

Article Title: Molecular breeding of polymerases for resistance to environmental inhibitors
Article Snippet: PCR screening was performed using primers (5′-AAA AAT CTA GAT AAC GAG GGC AA/5′-ACC ACC GAA CTG CGG GTG ACG CCA AGC G) and 0.01 µl of pASK75 as template (100 µM stock), and the following cycling conditions: 94°C 1 min, 25× (94°C 10 s, 50°C 30 s, 72°C 1 min), 65°C 2 min. Lysates which resulted in an AP (representing active polymerase clones) were then screened for their resistance towards the different inhibitors in inhibition assays. .. Promising polymerase clones were expressed and purified as described ( ) using a 16/10 Hi-Prep Heparin FF Column (Amersham Pharmacia Biotech).

Article Title: Molecular breeding of polymerases for amplification of ancient DNA
Article Snippet: .. Expression of polymerases for characterization and ancient DNA PCR was as described , but using a 16/10 Hi-Prep Heparin FF Column (Amersham Pharmacia Biotech) column to purify heat-cleared (50°C, 30min) Bugbuster (Novagen) lysate with Complete™ EDTA-free protease inhibitor cocktail (Roche). ..

Article Title: Evolving a polymerase for hydrophobic base analogues
Article Snippet: After selection rounds one and two, clones were screened by d5NIC PCR with primers 1,2 or 3,4 and by polymerase ELISA as described using hairpins 16–19 or 20–23. .. Expression of polymerases for characterization was as described using a 16/10 Hi-Prep Heparin FF Column (Amersham Pharmacia Biotech).

Purification:

Article Title: Molecular breeding of polymerases for resistance to environmental inhibitors
Article Snippet: .. Promising polymerase clones were expressed and purified as described ( ) using a 16/10 Hi-Prep Heparin FF Column (Amersham Pharmacia Biotech). ..

Enzyme-linked Immunosorbent Assay:

Article Title: Evolving a polymerase for hydrophobic base analogues
Article Snippet: After selection rounds one and two, clones were screened by d5NIC PCR with primers 1,2 or 3,4 and by polymerase ELISA as described using hairpins 16–19 or 20–23. .. Expression of polymerases for characterization was as described using a 16/10 Hi-Prep Heparin FF Column (Amersham Pharmacia Biotech).

CTG Assay:

Article Title: Molecular breeding of polymerases for resistance to environmental inhibitors
Article Snippet: PCR screening was performed using primers (5′-AAA AAT CTA GAT AAC GAG GGC AA/5′-ACC ACC GAA CTG CGG GTG ACG CCA AGC G) and 0.01 µl of pASK75 as template (100 µM stock), and the following cycling conditions: 94°C 1 min, 25× (94°C 10 s, 50°C 30 s, 72°C 1 min), 65°C 2 min. Lysates which resulted in an AP (representing active polymerase clones) were then screened for their resistance towards the different inhibitors in inhibition assays. .. Promising polymerase clones were expressed and purified as described ( ) using a 16/10 Hi-Prep Heparin FF Column (Amersham Pharmacia Biotech).

Selection:

Article Title: A polymerase engineered for bisulfite sequencing
Article Snippet: Polymerases Polymerases 3A10 ( ) and 5D4 ( ) were obtained by compartmentalized self-replication (CSR) selection ( ) and polymerase ( ) E10 by short-patch CSR selection ( ) as described. .. Expression of polymerases for characterization was as described ( , ) using a 16/10 Hi-Prep Heparin FF Column (Amersham Pharmacia Biotech).

Article Title: Evolving a polymerase for hydrophobic base analogues
Article Snippet: Paragraph title: Selection, screening, protein expression and characterization ... Expression of polymerases for characterization was as described using a 16/10 Hi-Prep Heparin FF Column (Amersham Pharmacia Biotech).

Activity Assay:

Article Title: Molecular breeding of polymerases for resistance to environmental inhibitors
Article Snippet: Promising polymerase clones were expressed and purified as described ( ) using a 16/10 Hi-Prep Heparin FF Column (Amersham Pharmacia Biotech). .. Purified polymerases were activity normalized to Taq (5 U/µl) using primers 5′-AAA AAT CTA GAT AAC GAG GGC AA/5′-ACC ACC GAA CTG CGG GTG ACG CCA AGC G, 0.01 µl of pASK75 as template (100 µM stock), and the following cycling conditions: 94°C, 1 min, 25× (94°C 10 s, 50°C 30 s, 72°C 1 min), 65°C 2 min.

Expressing:

Article Title: A polymerase engineered for bisulfite sequencing
Article Snippet: .. Expression of polymerases for characterization was as described ( , ) using a 16/10 Hi-Prep Heparin FF Column (Amersham Pharmacia Biotech). ..

Article Title: Molecular breeding of polymerases for resistance to environmental inhibitors
Article Snippet: Paragraph title: Screening, protein expression and characterization ... Promising polymerase clones were expressed and purified as described ( ) using a 16/10 Hi-Prep Heparin FF Column (Amersham Pharmacia Biotech).

Article Title: Molecular breeding of polymerases for amplification of ancient DNA
Article Snippet: .. Expression of polymerases for characterization and ancient DNA PCR was as described , but using a 16/10 Hi-Prep Heparin FF Column (Amersham Pharmacia Biotech) column to purify heat-cleared (50°C, 30min) Bugbuster (Novagen) lysate with Complete™ EDTA-free protease inhibitor cocktail (Roche). ..

Article Title: Evolving a polymerase for hydrophobic base analogues
Article Snippet: .. Expression of polymerases for characterization was as described using a 16/10 Hi-Prep Heparin FF Column (Amersham Pharmacia Biotech). ..

Sequencing:

Article Title: Evolving a polymerase for hydrophobic base analogues
Article Snippet: Selected mutations were reverted back to wild-type Taq sequence using Quickchange Mutagenesis using Pfu Turbo (Stratagene) and primers 31–40. .. Expression of polymerases for characterization was as described using a 16/10 Hi-Prep Heparin FF Column (Amersham Pharmacia Biotech).

Inhibition:

Article Title: Molecular breeding of polymerases for resistance to environmental inhibitors
Article Snippet: PCR screening was performed using primers (5′-AAA AAT CTA GAT AAC GAG GGC AA/5′-ACC ACC GAA CTG CGG GTG ACG CCA AGC G) and 0.01 µl of pASK75 as template (100 µM stock), and the following cycling conditions: 94°C 1 min, 25× (94°C 10 s, 50°C 30 s, 72°C 1 min), 65°C 2 min. Lysates which resulted in an AP (representing active polymerase clones) were then screened for their resistance towards the different inhibitors in inhibition assays. .. Promising polymerase clones were expressed and purified as described ( ) using a 16/10 Hi-Prep Heparin FF Column (Amersham Pharmacia Biotech).

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    GE Healthcare hi prep 16 10 heparin ff column
    Hi Prep 16 10 Heparin Ff Column, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hi prep 16 10 heparin ff column/product/GE Healthcare
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hi prep 16 10 heparin ff column - by Bioz Stars, 2020-04
    86/100 stars
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