Structured Review

Millipore 15n4
15n4, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/15n4/product/Millipore
Average 94 stars, based on 3 article reviews
Price from $9.99 to $1999.99
15n4 - by Bioz Stars, 2020-04
94/100 stars

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Modification:

Article Title: A Subcellular Quantitative Proteomic Analysis of Herpes Simplex Virus Type 1-Infected HEK 293T Cells
Article Snippet: Cell and Virus HEK 293T cells obtained from CCTCC (China Center for Type Culture Collection, Wuhan, China) were grown in Dulbecco’s modified Eagle’s medium (DMEM, GIBCO, Grand Island, NE, USA) supplemented with 1% penicillin/streptomycin (GIBCO) and 10% fetal bovine serum (FBS, GIBCO) at 37 °C with 5% CO2 . .. For SILAC labeling, HEK 293T cells were cultured in a SILAC medium (Thermo, Waltham, MA, USA) containing 10% dialyzed FBS (Thermo) and l -arginine and l -lysine (R0K0, “light”), l -arginine-13C6 and l -lysine-4,4,5,5-d4 (L) (R6K4, “medium”), or l- arginine-13C6, 15N4 and l -lysine-13C6, 15N2 (R10K8, “heavy”) (Sigma, St. Louis, MO, USA).

Labeling:

Article Title: Proteomics of a fuzzy organelle: interphase chromatin
Article Snippet: .. For SILAC labeling (Ong et al , ) of human cells, medium was supplemented with unlabeled amino acids (light label), with arginine:HCl, 13C6 (Cambridge Isotope Laboratories), and lysine:HCl, 4,4,5,5-d4 (Sigma) for the medium label, or with arginine:HCl 13C6, 15N4 and lysine:HCl, 13C6, 15N2 (Sigma) for the heavy label. ..

Article Title: A Subcellular Quantitative Proteomic Analysis of Herpes Simplex Virus Type 1-Infected HEK 293T Cells
Article Snippet: .. For SILAC labeling, HEK 293T cells were cultured in a SILAC medium (Thermo, Waltham, MA, USA) containing 10% dialyzed FBS (Thermo) and l -arginine and l -lysine (R0K0, “light”), l -arginine-13C6 and l -lysine-4,4,5,5-d4 (L) (R6K4, “medium”), or l- arginine-13C6, 15N4 and l -lysine-13C6, 15N2 (R10K8, “heavy”) (Sigma, St. Louis, MO, USA). .. Cells were cultured in the SILAC medium for at least seven doublings to ensure that the labeled amino acids were incorporated into cellular proteins.

Cell Culture:

Article Title: Proteomics of a fuzzy organelle: interphase chromatin
Article Snippet: Paragraph title: Cell culture, SILAC labeling, and treatments ... For SILAC labeling (Ong et al , ) of human cells, medium was supplemented with unlabeled amino acids (light label), with arginine:HCl, 13C6 (Cambridge Isotope Laboratories), and lysine:HCl, 4,4,5,5-d4 (Sigma) for the medium label, or with arginine:HCl 13C6, 15N4 and lysine:HCl, 13C6, 15N2 (Sigma) for the heavy label.

Article Title: A Subcellular Quantitative Proteomic Analysis of Herpes Simplex Virus Type 1-Infected HEK 293T Cells
Article Snippet: .. For SILAC labeling, HEK 293T cells were cultured in a SILAC medium (Thermo, Waltham, MA, USA) containing 10% dialyzed FBS (Thermo) and l -arginine and l -lysine (R0K0, “light”), l -arginine-13C6 and l -lysine-4,4,5,5-d4 (L) (R6K4, “medium”), or l- arginine-13C6, 15N4 and l -lysine-13C6, 15N2 (R10K8, “heavy”) (Sigma, St. Louis, MO, USA). .. Cells were cultured in the SILAC medium for at least seven doublings to ensure that the labeled amino acids were incorporated into cellular proteins.

Infection:

Article Title: A Subcellular Quantitative Proteomic Analysis of Herpes Simplex Virus Type 1-Infected HEK 293T Cells
Article Snippet: For SILAC labeling, HEK 293T cells were cultured in a SILAC medium (Thermo, Waltham, MA, USA) containing 10% dialyzed FBS (Thermo) and l -arginine and l -lysine (R0K0, “light”), l -arginine-13C6 and l -lysine-4,4,5,5-d4 (L) (R6K4, “medium”), or l- arginine-13C6, 15N4 and l -lysine-13C6, 15N2 (R10K8, “heavy”) (Sigma, St. Louis, MO, USA). .. Cells cultured in different mediums were infected with HSV-1 at an MOI of 5 or mock treated, and harvested at 4 or 20 h p.i.

Expressing:

Article Title: Proteomics of a fuzzy organelle: interphase chromatin
Article Snippet: For SILAC labeling (Ong et al , ) of human cells, medium was supplemented with unlabeled amino acids (light label), with arginine:HCl, 13C6 (Cambridge Isotope Laboratories), and lysine:HCl, 4,4,5,5-d4 (Sigma) for the medium label, or with arginine:HCl 13C6, 15N4 and lysine:HCl, 13C6, 15N2 (Sigma) for the heavy label. .. HeLa Tet-On cells expressing a Flag- and HA-tagged version of macroH2A have been described (Timinszky et al , ).

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    Millipore carbamazepine
    Fasted Simulated Intestinal Fluid (FaSSIF) mini-dissolution curves for <t>carbamazepine</t> loaded silica (left) and carbamazepine HME formulation (right) showing crystalline carbamazepine (♦), unstressed carbamazepine formulation (●), and stressed carbamazepine formulations at 30 (▪), 60 (X), and 90 (▲) days.
    Carbamazepine, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/carbamazepine/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    carbamazepine - by Bioz Stars, 2020-04
    99/100 stars
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    Fasted Simulated Intestinal Fluid (FaSSIF) mini-dissolution curves for carbamazepine loaded silica (left) and carbamazepine HME formulation (right) showing crystalline carbamazepine (♦), unstressed carbamazepine formulation (●), and stressed carbamazepine formulations at 30 (▪), 60 (X), and 90 (▲) days.

    Journal: Pharmaceutics

    Article Title: Opportunities for Successful Stabilization of Poor Glass-Forming Drugs: A Stability-Based Comparison of Mesoporous Silica Versus Hot Melt Extrusion Technologies

    doi: 10.3390/pharmaceutics11110577

    Figure Lengend Snippet: Fasted Simulated Intestinal Fluid (FaSSIF) mini-dissolution curves for carbamazepine loaded silica (left) and carbamazepine HME formulation (right) showing crystalline carbamazepine (♦), unstressed carbamazepine formulation (●), and stressed carbamazepine formulations at 30 (▪), 60 (X), and 90 (▲) days.

    Article Snippet: Materials Haloperidol, carbamazepine, HPLC grade acetonitrile, and HPLC grade methanol were purchased from MilliporeSigma (Darmstadt, Germany).

    Techniques:

    13 C SS-NMR spectra for crystalline carbamazepine ( c ) and carbamazepine HME formulation at 0 days ( b ) and 90 days ( a ).

    Journal: Pharmaceutics

    Article Title: Opportunities for Successful Stabilization of Poor Glass-Forming Drugs: A Stability-Based Comparison of Mesoporous Silica Versus Hot Melt Extrusion Technologies

    doi: 10.3390/pharmaceutics11110577

    Figure Lengend Snippet: 13 C SS-NMR spectra for crystalline carbamazepine ( c ) and carbamazepine HME formulation at 0 days ( b ) and 90 days ( a ).

    Article Snippet: Materials Haloperidol, carbamazepine, HPLC grade acetonitrile, and HPLC grade methanol were purchased from MilliporeSigma (Darmstadt, Germany).

    Techniques: Nuclear Magnetic Resonance

    Powder X-ray (PXRD) patterns for carbamazepine loaded silica (top) and carbamazepine HME (bottom) showing crystalline carbamazepine (a), pure Parteck MXP ® (PVA) (b), unstressed carbamazepine formulation (c) and stressed carbamazepine formulations at 30 (d), 60 (e), and 90 (f) days. The arrows indicate crystalline peaks in the diffractograms.

    Journal: Pharmaceutics

    Article Title: Opportunities for Successful Stabilization of Poor Glass-Forming Drugs: A Stability-Based Comparison of Mesoporous Silica Versus Hot Melt Extrusion Technologies

    doi: 10.3390/pharmaceutics11110577

    Figure Lengend Snippet: Powder X-ray (PXRD) patterns for carbamazepine loaded silica (top) and carbamazepine HME (bottom) showing crystalline carbamazepine (a), pure Parteck MXP ® (PVA) (b), unstressed carbamazepine formulation (c) and stressed carbamazepine formulations at 30 (d), 60 (e), and 90 (f) days. The arrows indicate crystalline peaks in the diffractograms.

    Article Snippet: Materials Haloperidol, carbamazepine, HPLC grade acetonitrile, and HPLC grade methanol were purchased from MilliporeSigma (Darmstadt, Germany).

    Techniques:

    Haloperidol (top) and carbamazepine (bottom) hot melt extrusion (HME) before (left) and after (right) 7 days accelerated stability conditions as specified in the materials and methods section.

    Journal: Pharmaceutics

    Article Title: Opportunities for Successful Stabilization of Poor Glass-Forming Drugs: A Stability-Based Comparison of Mesoporous Silica Versus Hot Melt Extrusion Technologies

    doi: 10.3390/pharmaceutics11110577

    Figure Lengend Snippet: Haloperidol (top) and carbamazepine (bottom) hot melt extrusion (HME) before (left) and after (right) 7 days accelerated stability conditions as specified in the materials and methods section.

    Article Snippet: Materials Haloperidol, carbamazepine, HPLC grade acetonitrile, and HPLC grade methanol were purchased from MilliporeSigma (Darmstadt, Germany).

    Techniques:

    SEM images for carbamazepine loaded silica ( a ) and HME ( b ) showing particle size and morphology at 0 days (top) and 90 days stability (bottom) as specified in the materials and methods section.

    Journal: Pharmaceutics

    Article Title: Opportunities for Successful Stabilization of Poor Glass-Forming Drugs: A Stability-Based Comparison of Mesoporous Silica Versus Hot Melt Extrusion Technologies

    doi: 10.3390/pharmaceutics11110577

    Figure Lengend Snippet: SEM images for carbamazepine loaded silica ( a ) and HME ( b ) showing particle size and morphology at 0 days (top) and 90 days stability (bottom) as specified in the materials and methods section.

    Article Snippet: Materials Haloperidol, carbamazepine, HPLC grade acetonitrile, and HPLC grade methanol were purchased from MilliporeSigma (Darmstadt, Germany).

    Techniques: