repli g single cell kit (Qiagen)
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Name:
REPLI-g Single Cell Kit
Description:
For highly uniform whole genome amplification (WGA) from single cells or limited sample material. Kit contents: Qiagen REPLI-g Single Cell Kit, 24 WGA rxns, 2 to 1000 Cells Input Amount, 15 min. Hands-on Time, 8 to 16 hr. Reaction Time, 40g/rxns Yields, MDA Technology, Tube Format, For Highly Uniform Whole Genome Amplification (WGA) from Single Cells or Limited Sample, Includes REPLI-g sc Polymerase, Buffers and Reagents. Benefits: WGA from single cell material with complete genome coverage. Unbiased amplification of genomic loci due to MDA technology. Optimized for use with new technologies, including NGS. Consistent yields of up to 40 µg (average product length >10 kb). Novel tool for cancer research, stem cell research, or metagenomics
Catalog Number:
150343
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None
Category:
REPLI-g Single Cell Kit
Score:
85
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Structured Review
Qiagen
repli g single cell kit
For highly uniform whole genome amplification (WGA) from single cells or limited sample material. Kit contents: Qiagen REPLI-g Single Cell Kit, 24 WGA rxns, 2 to 1000 Cells Input Amount, 15 min. Hands-on Time, 8 to 16 hr. Reaction Time, 40g/rxns Yields, MDA Technology, Tube Format, For Highly Uniform Whole Genome Amplification (WGA) from Single Cells or Limited Sample, Includes REPLI-g sc Polymerase, Buffers and Reagents. Benefits: WGA from single cell material with complete genome coverage. Unbiased amplification of genomic loci due to MDA technology. Optimized for use with new technologies, including NGS. Consistent yields of up to 40 µg (average product length >10 kb). Novel tool for cancer research, stem cell research, or metagenomics
https://www.bioz.com/result/repli g single cell kit/product/Qiagen
Average 99 stars, based on 33 article reviews
Price from $9.99 to $1999.99
For highly uniform whole genome amplification (WGA) from single cells or limited sample material. Kit contents: Qiagen REPLI-g Single Cell Kit, 24 WGA rxns, 2 to 1000 Cells Input Amount, 15 min. Hands-on Time, 8 to 16 hr. Reaction Time, 40g/rxns Yields, MDA Technology, Tube Format, For Highly Uniform Whole Genome Amplification (WGA) from Single Cells or Limited Sample, Includes REPLI-g sc Polymerase, Buffers and Reagents. Benefits: WGA from single cell material with complete genome coverage. Unbiased amplification of genomic loci due to MDA technology. Optimized for use with new technologies, including NGS. Consistent yields of up to 40 µg (average product length >10 kb). Novel tool for cancer research, stem cell research, or metagenomics
https://www.bioz.com/result/repli g single cell kit/product/Qiagen
Average 99 stars, based on 33 article reviews
Price from $9.99 to $1999.99
repli g single cell kit - by Bioz Stars,
2019-12
99/100 stars
Related Products / Commonly Used Together
Images
1) Product Images from "Comparative study of whole genome amplification and next generation sequencing performance of single cancer cells"
Article Title: Comparative study of whole genome amplification and next generation sequencing performance of single cancer cells
Journal: Oncotarget
doi: 10.18632/oncotarget.10701
Figure Legend Snippet: Plots of CNA profiles along the whole genome (x axis) ( A ) CNA profile of unamplified DNA from unfixed cells. ( B – G ) plots of CNAs in single SK-BR-3 cells, obtained from EDTA-preserved blood. ( H , I ) CNA profiles of individual CTCs, obtained from EDTA-preserved blood of the same breast cancer patient. WGA kits: (B, E) Ampli1; (C, F, H, I) PicoPlex; (D, G) REPLI-g.
Techniques Used: Whole Genome Amplification
Figure Legend Snippet: Distribution of identified known SNPs between datasets ( A ) Known SNPs identified in single cells, amplified with Ampli1, PicoPlex, and REPLI-g WGA kits and obtained from EDTA-preserved blood in comparison to unamplified DNA. ( B ) Known SNPs identified in single cells, amplified with Ampli1 or PicoPlex and obtained from EDTA- and CellSave-preserved blood in comparison to unamplified DNA from unfixed cells. ( C ) Known SNPs identified in single CTCs, amplified with PicoPlex in comparison to each other.
Techniques Used: Amplification, Whole Genome Amplification
2) Product Images from "Comparative study of whole genome amplification and next generation sequencing performance of single cancer cells"
Article Title: Comparative study of whole genome amplification and next generation sequencing performance of single cancer cells
Journal: Oncotarget
doi: 10.18632/oncotarget.10701
Figure Legend Snippet: Plots of CNA profiles along the whole genome (x axis) ( A ) CNA profile of unamplified DNA from unfixed cells. ( B – G ) plots of CNAs in single SK-BR-3 cells, obtained from EDTA-preserved blood. ( H , I ) CNA profiles of individual CTCs, obtained from EDTA-preserved blood of the same breast cancer patient. WGA kits: (B, E) Ampli1; (C, F, H, I) PicoPlex; (D, G) REPLI-g.
Techniques Used: Whole Genome Amplification
Figure Legend Snippet: Distribution of identified known SNPs between datasets ( A ) Known SNPs identified in single cells, amplified with Ampli1, PicoPlex, and REPLI-g WGA kits and obtained from EDTA-preserved blood in comparison to unamplified DNA. ( B ) Known SNPs identified in single cells, amplified with Ampli1 or PicoPlex and obtained from EDTA- and CellSave-preserved blood in comparison to unamplified DNA from unfixed cells. ( C ) Known SNPs identified in single CTCs, amplified with PicoPlex in comparison to each other.
Techniques Used: Amplification, Whole Genome Amplification
Related Articles
Amplification:Article Title: A tripartite survey of hyperparasitic fungi associated with ectoparasitic flies on bats (Mammalia: Chiroptera) in a neotropical cloud forest in Panama Article Snippet: Paragraph title: DNA extraction, amplification, phylogenetic analysis ... DNA was extracted from 1-4 Laboulbeniales thalli using a modified Article Title: Tracking heavy water (D2O) incorporation for identifying and sorting active microbial cells Article Snippet: The Article Title: Comparison of variations detection between whole-genome amplification methods used in single-cell resequencing Article Snippet: We obtained 29 single cells from the YH cell line (a human lymphoblastoid cell line from first Asian genome donor [ ]) and amplified them using seven commercialized kits. .. The kits tested were: GenomePlex® Single Cell WGA Kit (which we called DOP-1, Sigma-Aldrich, St. Louis, MO, USA); Silicon Biosystem Ampli™ WGA Kit (DOP-2, Silicon Biosystems, Bologna, Italy); NEB Single Cell WGA Kit (DOP-3, New England Biolabs, Ipswich, MA, USA); Qiagen REPLI-g Mini Kit (MDA-1, Qiagen, Düsseldorf, Germany); Qiagen Article Title: Evolution of multiple cell clones over a 29-year period of a CLL patient Article Snippet: Single CLL tumour cells are selected by a Micromanipulator (Eppendorf TransferMan NK2) under the inverted fluorescence microscope (Olympus IX-71), and pipetted into PCR tubes that contained 2 μl lysis buffer. .. Single-cell DNA amplification was carried out using the Article Title: Development of a facile droplet-based single-cell isolation platform for cultivation and genomic analysis in microorganisms Article Snippet: Cells were recovered from the droplets as described above and were lysed by adding 1.5 μl of buffer D2 (REPLI-g Single Cell Kit; Qiagen, USA) containing 0.08 mol/L dithiothreitol (DTT) and incubation at 65 o C for 10 minutes, followed by neutralization with 1.5 μl of Stop Solution ( Article Title: Performance of four modern whole genome amplification methods for copy number variant detection in single cells Article Snippet: For the bulk DNA sample, DNA was extracted from 5 * 106 cells using the DNeasy Blood & Tissue kit (Qiagen Hilden, Germany). .. Cell lysis and amplification was performed, using the Ampli-1 WGA kit (Silicon Biosystems, Castel Maggiore, Italy), the Article Title: Microfluidic-based mini-metagenomics enables discovery of novel microbial lineages from complex environmental samples Article Snippet: Paragraph title: Microfluidic genomic DNA amplification on Fluidigm C1 auto prep system ... After alkaline denaturation of DNA, neutralization and MDA were performed (Qiagen Article Title: Precision oncology using a limited number of cells: optimization of whole genome amplification products for sequencing applications Article Snippet: Isolated cells were subjected to genomic DNA isolation and WGA using one of the three commercially available single-cell WGA kits according to the manufacturer’s protocol: Article Title: A viability-linked metagenomic analysis of cleanroom environments: eukarya, prokaryotes, and viruses Article Snippet: All manipulations were performed in a bleach-cleaned biohood, which resided in an ultra-clean laboratory environment (i.e., single-use lab coats, bleached gloves, booties, etc.). .. Each sample was divided into 1 μl aliquots, which were amplified via Multiple Displacement Amplification (MDA) using Article Title: Impact of Contaminating DNA in Whole-Genome Amplification Kits Used for Metagenomic Shotgun Sequencing for Infection Diagnosis Article Snippet: Whole-genome amplification (WGA) is a useful tool for amplification of very small quantities of DNA for many uses, including metagenomic shotgun sequencing for infection diagnosis. .. The Illustra V2 Genomiphi, Illustra single cell Genomiphi, and Qiagen Article Title: CHROMOTHRIPSIS FROM DNA DAMAGE IN MICRONUCLEI Article Snippet: Paragraph title: Multi-strand displacement amplification and library construction of single-cell genomic DNA ... DNA from isolated cells was subject to MDA following lysis using the REPLI-g Single Cell Kit (Qiagen) with minor modification. (Note that we achieved the best overall coverage uniformity with this latest version of Article Title: FMR1 CGG repeat expansion mutation detection and linked haplotype analysis for reliable and accurate preimplantation genetic diagnosis of fragile X syndrome Article Snippet: Single cells isolated from lymphoblastoid cell lines (Coriell Cell Repositories, CCR; Camden, New Jersey, USA) were used to validate the FMR1 TP-PCR and tetradecaplex marker PCR assays for direct CGG repeat and linked multi-marker haplotype analyses, respectively. .. Isolation, lysis and/or whole-genome amplification, using the Genomiphi™ V2 DNA (GE Healthcare, Little Chalfont, UK) or Whole Genome Amplification:Article Title: Ultrahigh-throughput functional profiling of microbiota communities Article Snippet: The selected MDE droplets were freeze dried and total DNA was isolated using the QIAamp DNA Investigator Kit (Qiagen). .. Whole-genome amplification was performed using the Article Title: Germinal center reentries of BCL2-overexpressing B cells drive follicular lymphoma progression Article Snippet: The 50-Mb mouse exome was captured using Agilent SureSelect XT, genome version GRCm38/mm10. .. Capture libraries were constructed from 1 μg of WGA-amplified DNA from GC and post-GC subsets (BCL2-enriched or control empty vector) and germlines (naive) using the Article Title: Comparison of variations detection between whole-genome amplification methods used in single-cell resequencing Article Snippet: We obtained 29 single cells from the YH cell line (a human lymphoblastoid cell line from first Asian genome donor [ ]) and amplified them using seven commercialized kits. .. The kits tested were: GenomePlex® Single Cell WGA Kit (which we called DOP-1, Sigma-Aldrich, St. Louis, MO, USA); Silicon Biosystem Ampli™ WGA Kit (DOP-2, Silicon Biosystems, Bologna, Italy); NEB Single Cell WGA Kit (DOP-3, New England Biolabs, Ipswich, MA, USA); Qiagen REPLI-g Mini Kit (MDA-1, Qiagen, Düsseldorf, Germany); Qiagen Article Title: Performance of four modern whole genome amplification methods for copy number variant detection in single cells Article Snippet: For the bulk DNA sample, DNA was extracted from 5 * 106 cells using the DNeasy Blood & Tissue kit (Qiagen Hilden, Germany). .. Cell lysis and amplification was performed, using the Ampli-1 WGA kit (Silicon Biosystems, Castel Maggiore, Italy), the Article Title: Microfluidic-based mini-metagenomics enables discovery of novel microbial lineages from complex environmental samples Article Snippet: Following cell loading, whole genome amplification via MDA was performed on-chip in 96 independent reactions. .. After alkaline denaturation of DNA, neutralization and MDA were performed (Qiagen Article Title: Precision oncology using a limited number of cells: optimization of whole genome amplification products for sequencing applications Article Snippet: Cell transfer to the tube cap was confirmed by imaging the cap prior to cap closure using the cap-check function. .. Isolated cells were subjected to genomic DNA isolation and WGA using one of the three commercially available single-cell WGA kits according to the manufacturer’s protocol: Article Title: A viability-linked metagenomic analysis of cleanroom environments: eukarya, prokaryotes, and viruses Article Snippet: All manipulations were performed in a bleach-cleaned biohood, which resided in an ultra-clean laboratory environment (i.e., single-use lab coats, bleached gloves, booties, etc.). .. 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(Note that we achieved the best overall coverage uniformity with this latest version of Article Title: FMR1 CGG repeat expansion mutation detection and linked haplotype analysis for reliable and accurate preimplantation genetic diagnosis of fragile X syndrome Article Snippet: Single cells isolated from lymphoblastoid cell lines (Coriell Cell Repositories, CCR; Camden, New Jersey, USA) were used to validate the FMR1 TP-PCR and tetradecaplex marker PCR assays for direct CGG repeat and linked multi-marker haplotype analyses, respectively. .. Isolation, lysis and/or whole-genome amplification, using the Genomiphi™ V2 DNA (GE Healthcare, Little Chalfont, UK) or Neutralization:Article Title: Development of a facile droplet-based single-cell isolation platform for cultivation and genomic analysis in microorganisms Article Snippet: Sixty droplets each with a single S. cerevisiae cell and twenty blank droplets were then collected successively in these wells. .. Cells were recovered from the droplets as described above and were lysed by adding 1.5 μl of buffer D2 ( Article Title: Microfluidic-based mini-metagenomics enables discovery of novel microbial lineages from complex environmental samples Article Snippet: A lysozyme (Epicenter) digestion step was added before alkaline denaturation of DNA. .. After alkaline denaturation of DNA, neutralization and MDA were performed (Qiagen Cytometry:Article Title: DNA methyltransferase 3b silencing affects locus-specific DNA methylation and inhibits proliferation, migration and invasion in human hepatocellular carcinoma SMMC-7721 and BEL-7402 cells Article Snippet: Paragraph title: Cell cycle and apoptosis flow cytometry ... Cell apoptosis and cell cycle distribution were finally evaluated using the Annexin V-FITC/propidium iodide apoptosis detection kit (Qiagen) and Construct:Article Title: Germinal center reentries of BCL2-overexpressing B cells drive follicular lymphoma progression Article Snippet: The 50-Mb mouse exome was captured using Agilent SureSelect XT, genome version GRCm38/mm10. .. Capture libraries were constructed from 1 μg of WGA-amplified DNA from GC and post-GC subsets (BCL2-enriched or control empty vector) and germlines (naive) using the Real-time Polymerase Chain Reaction:Article Title: Development of a facile droplet-based single-cell isolation platform for cultivation and genomic analysis in microorganisms Article Snippet: Paragraph title: Real-time quantitative PCR (qPCR) of single-cell DNA ... Cells were recovered from the droplets as described above and were lysed by adding 1.5 μl of buffer D2 (REPLI-g Single Cell Kit; Qiagen, USA) containing 0.08 mol/L dithiothreitol (DTT) and incubation at 65 o C for 10 minutes, followed by neutralization with 1.5 μl of Stop Solution ( Incubation:Article Title: Development of a facile droplet-based single-cell isolation platform for cultivation and genomic analysis in microorganisms Article Snippet: Sixty droplets each with a single S. cerevisiae cell and twenty blank droplets were then collected successively in these wells. .. Cells were recovered from the droplets as described above and were lysed by adding 1.5 μl of buffer D2 ( Article Title: CHROMOTHRIPSIS FROM DNA DAMAGE IN MICRONUCLEI Article Snippet: DNA from isolated cells was subject to MDA following lysis using the REPLI-g Single Cell Kit (Qiagen) with minor modification. (Note that we achieved the best overall coverage uniformity with this latest version of Modification:Article Title: A tripartite survey of hyperparasitic fungi associated with ectoparasitic flies on bats (Mammalia: Chiroptera) in a neotropical cloud forest in Panama Article Snippet: Voucher slides are deposited at Farlow Herbarium (FH; Harvard University, Cambridge, MA, USA) and Herbario de la Universidad Autónoma de Chiriquí (UCH; David, Panamá). .. DNA was extracted from 1-4 Laboulbeniales thalli using a modified Article Title: Microfluidic-based mini-metagenomics enables discovery of novel microbial lineages from complex environmental samples Article Snippet: The diluted environmental sample was loaded onto the chip using a modified version of the loading protocol where washing was not performed, as the capture sites were too large for microbial cells. .. After alkaline denaturation of DNA, neutralization and MDA were performed (Qiagen Article Title: Precision oncology using a limited number of cells: optimization of whole genome amplification products for sequencing applications Article Snippet: Isolated cells were subjected to genomic DNA isolation and WGA using one of the three commercially available single-cell WGA kits according to the manufacturer’s protocol: Article Title: CHROMOTHRIPSIS FROM DNA DAMAGE IN MICRONUCLEI Article Snippet: Finally, the high processivity of Phi-29 polymerase consistently generates large amplicons above 10 kb , ; this enables us to perform Sanger sequencing on the MDA product after PCR to generate phasing information of rearrangements and validate their association with the missegregated chromosome, which is crucial in establishing the relationship between chromosomal rearrangements and DNA damage in the micronuclei. .. DNA from isolated cells was subject to MDA following lysis using the Flow Cytometry:Article Title: DNA methyltransferase 3b silencing affects locus-specific DNA methylation and inhibits proliferation, migration and invasion in human hepatocellular carcinoma SMMC-7721 and BEL-7402 cells Article Snippet: Paragraph title: Cell cycle and apoptosis flow cytometry ... Cell apoptosis and cell cycle distribution were finally evaluated using the Annexin V-FITC/propidium iodide apoptosis detection kit (Qiagen) and Gas Chromatography:Article Title: Germinal center reentries of BCL2-overexpressing B cells drive follicular lymphoma progression Article Snippet: The 50-Mb mouse exome was captured using Agilent SureSelect XT, genome version GRCm38/mm10. .. Capture libraries were constructed from 1 μg of WGA-amplified DNA from GC and post-GC subsets (BCL2-enriched or control empty vector) and germlines (naive) using the Sensitive Assay:Article Title: Performance of four modern whole genome amplification methods for copy number variant detection in single cells Article Snippet: Cell lysis and amplification was performed, using the Ampli-1 WGA kit (Silicon Biosystems, Castel Maggiore, Italy), the Infection:Article Title: Impact of Contaminating DNA in Whole-Genome Amplification Kits Used for Metagenomic Shotgun Sequencing for Infection Diagnosis Article Snippet: Whole-genome amplification (WGA) is a useful tool for amplification of very small quantities of DNA for many uses, including metagenomic shotgun sequencing for infection diagnosis. .. The Illustra V2 Genomiphi, Illustra single cell Genomiphi, and Qiagen DNA Sequencing:Article Title: Evolution of multiple cell clones over a 29-year period of a CLL patient Article Snippet: Paragraph title: Single-cell DNA sequencing ... Single-cell DNA amplification was carried out using the Polymerase Chain Reaction:Article Title: A tripartite survey of hyperparasitic fungi associated with ectoparasitic flies on bats (Mammalia: Chiroptera) in a neotropical cloud forest in Panama Article Snippet: DNA was extracted from 1-4 Laboulbeniales thalli using a modified Article Title: Tracking heavy water (D2O) incorporation for identifying and sorting active microbial cells Article Snippet: The Article Title: Evolution of multiple cell clones over a 29-year period of a CLL patient Article Snippet: Single CLL tumour cells are selected by a Micromanipulator (Eppendorf TransferMan NK2) under the inverted fluorescence microscope (Olympus IX-71), and pipetted into PCR tubes that contained 2 μl lysis buffer. .. Single-cell DNA amplification was carried out using the Article Title: Development of a facile droplet-based single-cell isolation platform for cultivation and genomic analysis in microorganisms Article Snippet: Eighty wells of a 96-well PCR plate were each filled with 1 μl of PBS buffer (pH 8.0) before single-cell droplet collection. .. Cells were recovered from the droplets as described above and were lysed by adding 1.5 μl of buffer D2 (REPLI-g Single Cell Kit; Qiagen, USA) containing 0.08 mol/L dithiothreitol (DTT) and incubation at 65 o C for 10 minutes, followed by neutralization with 1.5 μl of Stop Solution ( Article Title: CHROMOTHRIPSIS FROM DNA DAMAGE IN MICRONUCLEI Article Snippet: Finally, the high processivity of Phi-29 polymerase consistently generates large amplicons above 10 kb , ; this enables us to perform Sanger sequencing on the MDA product after PCR to generate phasing information of rearrangements and validate their association with the missegregated chromosome, which is crucial in establishing the relationship between chromosomal rearrangements and DNA damage in the micronuclei. .. DNA from isolated cells was subject to MDA following lysis using the REPLI-g Single Cell Kit (Qiagen) with minor modification. (Note that we achieved the best overall coverage uniformity with this latest version of Article Title: FMR1 CGG repeat expansion mutation detection and linked haplotype analysis for reliable and accurate preimplantation genetic diagnosis of fragile X syndrome Article Snippet: Single cells isolated from lymphoblastoid cell lines (Coriell Cell Repositories, CCR; Camden, New Jersey, USA) were used to validate the FMR1 TP-PCR and tetradecaplex marker PCR assays for direct CGG repeat and linked multi-marker haplotype analyses, respectively. .. Isolation, lysis and/or whole-genome amplification, using the Genomiphi™ V2 DNA (GE Healthcare, Little Chalfont, UK) or Sonication:Article Title: Impact of Contaminating DNA in Whole-Genome Amplification Kits Used for Metagenomic Shotgun Sequencing for Infection Diagnosis Article Snippet: Three WGA kits were tested for their utility in a metagenomics approach to identify the pathogens in sonicate fluid comprised of biofilms and other materials dislodged from the surfaces of explanted prosthetic joints using sonication. .. The Illustra V2 Genomiphi, Illustra single cell Genomiphi, and Qiagen DNA Extraction:Article Title: A tripartite survey of hyperparasitic fungi associated with ectoparasitic flies on bats (Mammalia: Chiroptera) in a neotropical cloud forest in Panama Article Snippet: Paragraph title: DNA extraction, amplification, phylogenetic analysis ... DNA was extracted from 1-4 Laboulbeniales thalli using a modified Article Title: Precision oncology using a limited number of cells: optimization of whole genome amplification products for sequencing applications Article Snippet: Cell transfer to the tube cap was confirmed by imaging the cap prior to cap closure using the cap-check function. .. Isolated cells were subjected to genomic DNA isolation and WGA using one of the three commercially available single-cell WGA kits according to the manufacturer’s protocol: Article Title: Genomic Comparison of Campylobacter spp. and Their Potential for Zoonotic Transmission between Birds, Primates, and Livestock Article Snippet: Paragraph title: DNA extraction, library preparation, and next-generation sequencing. ... DNA was extracted using Fluorescence:Article Title: Evolution of multiple cell clones over a 29-year period of a CLL patient Article Snippet: Single CLL tumour cells are selected by a Micromanipulator (Eppendorf TransferMan NK2) under the inverted fluorescence microscope (Olympus IX-71), and pipetted into PCR tubes that contained 2 μl lysis buffer. .. Single-cell DNA amplification was carried out using the Multiple Displacement Amplification:Article Title: Tracking heavy water (D2O) incorporation for identifying and sorting active microbial cells Article Snippet: To wash off any cells that might have stuck to the walls of the capillary, 2 μL of sterile PBS buffer was then added to the capillary piece and the tube was centrifuged again. .. The Article Title: Comparison of variations detection between whole-genome amplification methods used in single-cell resequencing Article Snippet: We obtained 29 single cells from the YH cell line (a human lymphoblastoid cell line from first Asian genome donor [ ]) and amplified them using seven commercialized kits. .. The kits tested were: GenomePlex® Single Cell WGA Kit (which we called DOP-1, Sigma-Aldrich, St. Louis, MO, USA); Silicon Biosystem Ampli™ WGA Kit (DOP-2, Silicon Biosystems, Bologna, Italy); NEB Single Cell WGA Kit (DOP-3, New England Biolabs, Ipswich, MA, USA); Qiagen REPLI-g Mini Kit (MDA-1, Qiagen, Düsseldorf, Germany); Qiagen Article Title: Microfluidic-based mini-metagenomics enables discovery of novel microbial lineages from complex environmental samples Article Snippet: A lysozyme (Epicenter) digestion step was added before alkaline denaturation of DNA. .. After alkaline denaturation of DNA, neutralization and MDA were performed (Qiagen Article Title: Precision oncology using a limited number of cells: optimization of whole genome amplification products for sequencing applications Article Snippet: Isolated cells were subjected to genomic DNA isolation and WGA using one of the three commercially available single-cell WGA kits according to the manufacturer’s protocol: Article Title: A viability-linked metagenomic analysis of cleanroom environments: eukarya, prokaryotes, and viruses Article Snippet: All manipulations were performed in a bleach-cleaned biohood, which resided in an ultra-clean laboratory environment (i.e., single-use lab coats, bleached gloves, booties, etc.). .. Each sample was divided into 1 μl aliquots, which were amplified via Multiple Displacement Amplification (MDA) using Article Title: CHROMOTHRIPSIS FROM DNA DAMAGE IN MICRONUCLEI Article Snippet: Finally, the high processivity of Phi-29 polymerase consistently generates large amplicons above 10 kb , ; this enables us to perform Sanger sequencing on the MDA product after PCR to generate phasing information of rearrangements and validate their association with the missegregated chromosome, which is crucial in establishing the relationship between chromosomal rearrangements and DNA damage in the micronuclei. .. DNA from isolated cells was subject to MDA following lysis using the Isolation:Article Title: Ultrahigh-throughput functional profiling of microbiota communities Article Snippet: The selected MDE droplets were freeze dried and total DNA was isolated using the QIAamp DNA Investigator Kit (Qiagen). .. Whole-genome amplification was performed using the Article Title: Precision oncology using a limited number of cells: optimization of whole genome amplification products for sequencing applications Article Snippet: Cell transfer to the tube cap was confirmed by imaging the cap prior to cap closure using the cap-check function. .. Isolated cells were subjected to genomic DNA isolation and WGA using one of the three commercially available single-cell WGA kits according to the manufacturer’s protocol: Article Title: CHROMOTHRIPSIS FROM DNA DAMAGE IN MICRONUCLEI Article Snippet: Finally, the high processivity of Phi-29 polymerase consistently generates large amplicons above 10 kb , ; this enables us to perform Sanger sequencing on the MDA product after PCR to generate phasing information of rearrangements and validate their association with the missegregated chromosome, which is crucial in establishing the relationship between chromosomal rearrangements and DNA damage in the micronuclei. .. DNA from isolated cells was subject to MDA following lysis using the Article Title: FMR1 CGG repeat expansion mutation detection and linked haplotype analysis for reliable and accurate preimplantation genetic diagnosis of fragile X syndrome Article Snippet: Single cells isolated from lymphoblastoid cell lines (Coriell Cell Repositories, CCR; Camden, New Jersey, USA) were used to validate the FMR1 TP-PCR and tetradecaplex marker PCR assays for direct CGG repeat and linked multi-marker haplotype analyses, respectively. .. Isolation, lysis and/or whole-genome amplification, using the Genomiphi™ V2 DNA (GE Healthcare, Little Chalfont, UK) or Article Title: Genomic Comparison of Campylobacter spp. and Their Potential for Zoonotic Transmission between Birds, Primates, and Livestock Article Snippet: High-molecular-weight genomic DNA (gDNA) was isolated from bacterial colonies grown on 5% SBA plates (UC Davis Vet Med Biological Services) at 37°C in microaerophilic conditions (as described above). .. DNA was extracted using Microscopy:Article Title: Evolution of multiple cell clones over a 29-year period of a CLL patient Article Snippet: Single CLL tumour cells are selected by a Micromanipulator (Eppendorf TransferMan NK2) under the inverted fluorescence microscope (Olympus IX-71), and pipetted into PCR tubes that contained 2 μl lysis buffer. .. Single-cell DNA amplification was carried out using the Purification:Article Title: A tripartite survey of hyperparasitic fungi associated with ectoparasitic flies on bats (Mammalia: Chiroptera) in a neotropical cloud forest in Panama Article Snippet: DNA was extracted from 1-4 Laboulbeniales thalli using a modified Article Title: Evolution of multiple cell clones over a 29-year period of a CLL patient Article Snippet: Single-cell DNA amplification was carried out using the Article Title: Search for a Prion-Specific Nucleic Acid Article Snippet: The plates were irradiated at 4°C at 10 cm from the UV light source with an E max of 254 nm. .. The P3′SS vector was irradiated with 19.5 J/m2 (15 s) and 39 J/m2 (30 s), after which it was purified using the QIAGEN Article Title: Genomic Comparison of Campylobacter spp. and Their Potential for Zoonotic Transmission between Birds, Primates, and Livestock Article Snippet: DNA was extracted using Sequencing:Article Title: Ultrahigh-throughput functional profiling of microbiota communities Article Snippet: Paragraph title: NGS Sequencing. ... Whole-genome amplification was performed using the Article Title: Germinal center reentries of BCL2-overexpressing B cells drive follicular lymphoma progression Article Snippet: Paragraph title: Whole-exome mouse sequencing and analysis. ... Capture libraries were constructed from 1 μg of WGA-amplified DNA from GC and post-GC subsets (BCL2-enriched or control empty vector) and germlines (naive) using the Article Title: Comparison of variations detection between whole-genome amplification methods used in single-cell resequencing Article Snippet: The kits tested were: GenomePlex® Single Cell WGA Kit (which we called DOP-1, Sigma-Aldrich, St. Louis, MO, USA); Silicon Biosystem Ampli™ WGA Kit (DOP-2, Silicon Biosystems, Bologna, Italy); NEB Single Cell WGA Kit (DOP-3, New England Biolabs, Ipswich, MA, USA); Qiagen REPLI-g Mini Kit (MDA-1, Qiagen, Düsseldorf, Germany); Qiagen Article Title: Evolution of multiple cell clones over a 29-year period of a CLL patient Article Snippet: Single-cell DNA amplification was carried out using the Article Title: A viability-linked metagenomic analysis of cleanroom environments: eukarya, prokaryotes, and viruses Article Snippet: Paragraph title: Metagenomic sequencing ... Each sample was divided into 1 μl aliquots, which were amplified via Multiple Displacement Amplification (MDA) using Article Title: CHROMOTHRIPSIS FROM DNA DAMAGE IN MICRONUCLEI Article Snippet: Finally, the high processivity of Phi-29 polymerase consistently generates large amplicons above 10 kb , ; this enables us to perform Sanger sequencing on the MDA product after PCR to generate phasing information of rearrangements and validate their association with the missegregated chromosome, which is crucial in establishing the relationship between chromosomal rearrangements and DNA damage in the micronuclei. .. DNA from isolated cells was subject to MDA following lysis using the REPLI-g Single Cell Kit (Qiagen) with minor modification. (Note that we achieved the best overall coverage uniformity with this latest version of Blocking Assay:Article Title: Microfluidic-based mini-metagenomics enables discovery of novel microbial lineages from complex environmental samples Article Snippet: Priming the C1 IFC involved filling all microfluidic control channels with C1 Harvest Reagent, all capture sites with C1 Blocking Reagent, and the input multiplexer with C1 Preloading Reagent. .. After alkaline denaturation of DNA, neutralization and MDA were performed (Qiagen Shotgun Sequencing:Article Title: Impact of Contaminating DNA in Whole-Genome Amplification Kits Used for Metagenomic Shotgun Sequencing for Infection Diagnosis Article Snippet: Whole-genome amplification (WGA) is a useful tool for amplification of very small quantities of DNA for many uses, including metagenomic shotgun sequencing for infection diagnosis. .. The Illustra V2 Genomiphi, Illustra single cell Genomiphi, and Qiagen Chromatin Immunoprecipitation:Article Title: Microfluidic-based mini-metagenomics enables discovery of novel microbial lineages from complex environmental samples Article Snippet: The diluted environmental sample was loaded onto the chip using a modified version of the loading protocol where washing was not performed, as the capture sites were too large for microbial cells. .. After alkaline denaturation of DNA, neutralization and MDA were performed (Qiagen Plasmid Preparation:Article Title: Germinal center reentries of BCL2-overexpressing B cells drive follicular lymphoma progression Article Snippet: The 50-Mb mouse exome was captured using Agilent SureSelect XT, genome version GRCm38/mm10. .. Capture libraries were constructed from 1 μg of WGA-amplified DNA from GC and post-GC subsets (BCL2-enriched or control empty vector) and germlines (naive) using the Article Title: Search for a Prion-Specific Nucleic Acid Article Snippet: The plates were irradiated at 4°C at 10 cm from the UV light source with an E max of 254 nm. .. The P3′SS vector was irradiated with 19.5 J/m2 (15 s) and 39 J/m2 (30 s), after which it was purified using the QIAGEN Irradiation:Article Title: Search for a Prion-Specific Nucleic Acid Article Snippet: The plates were irradiated at 4°C at 10 cm from the UV light source with an E max of 254 nm. .. The P3′SS vector was irradiated with 19.5 J/m2 (15 s) and 39 J/m2 (30 s), after which it was purified using the QIAGEN Selection:Article Title: Impact of Contaminating DNA in Whole-Genome Amplification Kits Used for Metagenomic Shotgun Sequencing for Infection Diagnosis Article Snippet: The Illustra V2 Genomiphi, Illustra single cell Genomiphi, and Qiagen Next-Generation Sequencing:Article Title: Ultrahigh-throughput functional profiling of microbiota communities Article Snippet: Paragraph title: NGS Sequencing. ... Whole-genome amplification was performed using the Article Title: Genomic Comparison of Campylobacter spp. and Their Potential for Zoonotic Transmission between Birds, Primates, and Livestock Article Snippet: Paragraph title: DNA extraction, library preparation, and next-generation sequencing. ... DNA was extracted using Multiple Annealing and Looping–Based Amplification Cycles:Article Title: Comparison of variations detection between whole-genome amplification methods used in single-cell resequencing Article Snippet: We obtained 29 single cells from the YH cell line (a human lymphoblastoid cell line from first Asian genome donor [ ]) and amplified them using seven commercialized kits. .. The kits tested were: GenomePlex® Single Cell WGA Kit (which we called DOP-1, Sigma-Aldrich, St. Louis, MO, USA); Silicon Biosystem Ampli™ WGA Kit (DOP-2, Silicon Biosystems, Bologna, Italy); NEB Single Cell WGA Kit (DOP-3, New England Biolabs, Ipswich, MA, USA); Qiagen REPLI-g Mini Kit (MDA-1, Qiagen, Düsseldorf, Germany); Qiagen Article Title: Precision oncology using a limited number of cells: optimization of whole genome amplification products for sequencing applications Article Snippet: Cell transfer to the tube cap was confirmed by imaging the cap prior to cap closure using the cap-check function. .. Isolated cells were subjected to genomic DNA isolation and WGA using one of the three commercially available single-cell WGA kits according to the manufacturer’s protocol: Article Title: CHROMOTHRIPSIS FROM DNA DAMAGE IN MICRONUCLEI Article Snippet: We chose to amplify single-cell genomes by multi-strand displacement amplification (MDA) with the Phi-29 polymerase for four main reasons: First, MDA gives better overall genome coverage than PCR-based methods and also gives comparable uniformity - to other methods such as MALBAC ; this is required for the detection of chromosomal rearrangements. .. DNA from isolated cells was subject to MDA following lysis using the REPLI-g Single Cell Kit (Qiagen) with minor modification. (Note that we achieved the best overall coverage uniformity with this latest version of Concentration Assay:Article Title: Performance of four modern whole genome amplification methods for copy number variant detection in single cells Article Snippet: Cell lysis and amplification was performed, using the Ampli-1 WGA kit (Silicon Biosystems, Castel Maggiore, Italy), the Marker:Article Title: FMR1 CGG repeat expansion mutation detection and linked haplotype analysis for reliable and accurate preimplantation genetic diagnosis of fragile X syndrome Article Snippet: Single cells isolated from lymphoblastoid cell lines (Coriell Cell Repositories, CCR; Camden, New Jersey, USA) were used to validate the FMR1 TP-PCR and tetradecaplex marker PCR assays for direct CGG repeat and linked multi-marker haplotype analyses, respectively. .. Isolation, lysis and/or whole-genome amplification, using the Genomiphi™ V2 DNA (GE Healthcare, Little Chalfont, UK) or Lysis:Article Title: Tracking heavy water (D2O) incorporation for identifying and sorting active microbial cells Article Snippet: To wash off any cells that might have stuck to the walls of the capillary, 2 μL of sterile PBS buffer was then added to the capillary piece and the tube was centrifuged again. .. The Article Title: Evolution of multiple cell clones over a 29-year period of a CLL patient Article Snippet: Single CLL tumour cells are selected by a Micromanipulator (Eppendorf TransferMan NK2) under the inverted fluorescence microscope (Olympus IX-71), and pipetted into PCR tubes that contained 2 μl lysis buffer. .. Single-cell DNA amplification was carried out using the Article Title: Performance of four modern whole genome amplification methods for copy number variant detection in single cells Article Snippet: For the bulk DNA sample, DNA was extracted from 5 * 106 cells using the DNeasy Blood & Tissue kit (Qiagen Hilden, Germany). .. Cell lysis and amplification was performed, using the Ampli-1 WGA kit (Silicon Biosystems, Castel Maggiore, Italy), the Article Title: Precision oncology using a limited number of cells: optimization of whole genome amplification products for sequencing applications Article Snippet: Isolated cells were subjected to genomic DNA isolation and WGA using one of the three commercially available single-cell WGA kits according to the manufacturer’s protocol: Article Title: A viability-linked metagenomic analysis of cleanroom environments: eukarya, prokaryotes, and viruses Article Snippet: Each sample was divided into 1 μl aliquots, which were amplified via Multiple Displacement Amplification (MDA) using Article Title: CHROMOTHRIPSIS FROM DNA DAMAGE IN MICRONUCLEI Article Snippet: Finally, the high processivity of Phi-29 polymerase consistently generates large amplicons above 10 kb , ; this enables us to perform Sanger sequencing on the MDA product after PCR to generate phasing information of rearrangements and validate their association with the missegregated chromosome, which is crucial in establishing the relationship between chromosomal rearrangements and DNA damage in the micronuclei. .. DNA from isolated cells was subject to MDA following lysis using the Article Title: FMR1 CGG repeat expansion mutation detection and linked haplotype analysis for reliable and accurate preimplantation genetic diagnosis of fragile X syndrome Article Snippet: Single cells isolated from lymphoblastoid cell lines (Coriell Cell Repositories, CCR; Camden, New Jersey, USA) were used to validate the FMR1 TP-PCR and tetradecaplex marker PCR assays for direct CGG repeat and linked multi-marker haplotype analyses, respectively. .. Isolation, lysis and/or whole-genome amplification, using the Genomiphi™ V2 DNA (GE Healthcare, Little Chalfont, UK) or Variant Assay:Article Title: Germinal center reentries of BCL2-overexpressing B cells drive follicular lymphoma progression Article Snippet: Capture libraries were constructed from 1 μg of WGA-amplified DNA from GC and post-GC subsets (BCL2-enriched or control empty vector) and germlines (naive) using the Environmental Sampling:Article Title: Microfluidic-based mini-metagenomics enables discovery of novel microbial lineages from complex environmental samples Article Snippet: The diluted environmental sample was loaded onto the chip using a modified version of the loading protocol where washing was not performed, as the capture sites were too large for microbial cells. .. After alkaline denaturation of DNA, neutralization and MDA were performed (Qiagen |
