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Merck & Co 150 mm sequant zic philic column
150 Mm Sequant Zic Philic Column, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/150 mm sequant zic philic column/product/Merck & Co
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
150 mm sequant zic philic column - by Bioz Stars, 2021-07
86/100 stars

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Liquid Chromatography with Mass Spectroscopy:

Article Title: Metabolic Analyses Revealed Time-Dependent Synergistic Killing by Colistin and Aztreonam Combination Against Multidrug-Resistant Acinetobacter baumannii
Article Snippet: The supernatants (0.3 mL) containing extracted metabolites were collected after centrifugation at 3,220 ×g and 4°C for 10 min, then further centrifuged at 14,000 ×g for 10 min to achieve particle-free samples (0.2 mL) for LC-MS analysis. .. LC-MS Analysis of Metabolites Based on a published method , metabolite samples were analyzed on a Q-Exactive Orbitrap mass spectrometer (Thermo Fisher) coupled to a Dionex U3000 high-performance liquid chromatography (HPLC) with a ZIC-pHILIC column (5 μm, polymeric, 150 × 4.6 mm; SeQuant, Merck). .. The MS system was operated in both positive and negative electro-spray ionisation (ESI) mode with a resolution at 35,000 and a detection range of 85 to 1,275 m/z .

Mass Spectrometry:

Article Title: Metabolic Analyses Revealed Time-Dependent Synergistic Killing by Colistin and Aztreonam Combination Against Multidrug-Resistant Acinetobacter baumannii
Article Snippet: The supernatants (0.3 mL) containing extracted metabolites were collected after centrifugation at 3,220 ×g and 4°C for 10 min, then further centrifuged at 14,000 ×g for 10 min to achieve particle-free samples (0.2 mL) for LC-MS analysis. .. LC-MS Analysis of Metabolites Based on a published method , metabolite samples were analyzed on a Q-Exactive Orbitrap mass spectrometer (Thermo Fisher) coupled to a Dionex U3000 high-performance liquid chromatography (HPLC) with a ZIC-pHILIC column (5 μm, polymeric, 150 × 4.6 mm; SeQuant, Merck). .. The MS system was operated in both positive and negative electro-spray ionisation (ESI) mode with a resolution at 35,000 and a detection range of 85 to 1,275 m/z .

High Performance Liquid Chromatography:

Article Title: Metabolic Analyses Revealed Time-Dependent Synergistic Killing by Colistin and Aztreonam Combination Against Multidrug-Resistant Acinetobacter baumannii
Article Snippet: The supernatants (0.3 mL) containing extracted metabolites were collected after centrifugation at 3,220 ×g and 4°C for 10 min, then further centrifuged at 14,000 ×g for 10 min to achieve particle-free samples (0.2 mL) for LC-MS analysis. .. LC-MS Analysis of Metabolites Based on a published method , metabolite samples were analyzed on a Q-Exactive Orbitrap mass spectrometer (Thermo Fisher) coupled to a Dionex U3000 high-performance liquid chromatography (HPLC) with a ZIC-pHILIC column (5 μm, polymeric, 150 × 4.6 mm; SeQuant, Merck). .. The MS system was operated in both positive and negative electro-spray ionisation (ESI) mode with a resolution at 35,000 and a detection range of 85 to 1,275 m/z .

Article Title: Acetate Recapturing by Nuclear Acetyl-CoA Synthetase 2 Prevents Loss of Histone Acetylation during Oxygen and Serum Limitation
Article Snippet: Analysis was performed with a Q-Exactive Orbitrap mass spectrometer (Thermo Scientific) and a Thermo Ultimate 3000 high-performance liquid chromatography (HPLC) system. .. HPLC consisted of a ZIC-pHILIC column (SeQuant, 150 × 2.1 mm, 5 μm, Merck) with a ZIC-pHILIC guard column (SeQuant, 20 × 2.1 mm) and an initial mobile phase of 20% 20 mM ammonium carbonate (pH 9.4), and 80% acetonitrile. ..

Article Title: Protective Role of Mytilus edulis Hydrolysate in Lipopolysaccharide-Galactosamine Acute Liver Injury
Article Snippet: The sustainability of the lab-scale pre-GMP production was confirmed by the IGMM (Institute of Genetics and Molecular Medicine) Mass Spectrometry laboratory (University of Edinburgh). .. N2-01 (in its undiluted form) was diluted 40:1 in 5:3:2 methanol:acetonitrile:water and 10 ul were injected onto a ZIC-pHILIC 4.6 mm x 150 mm Sequant column (Merck, United Kingdom) on an Ultimate 3000 series HPLC (Thermo Fisher Scientific, United States) with A gradient from 90 to 5% B in 20 min, where A was 20 mm ammonium carbonate and B was acetonitrile. .. Data were acquired on Q Exactive (Thermo Fisher Scientific, United States) with HESI in positive (75.5–1132.5) and negative (77.5–1132.5) mode at 70k resolution, with ddMS2 at 17.5k.

Injection:

Article Title: Protective Role of Mytilus edulis Hydrolysate in Lipopolysaccharide-Galactosamine Acute Liver Injury
Article Snippet: The sustainability of the lab-scale pre-GMP production was confirmed by the IGMM (Institute of Genetics and Molecular Medicine) Mass Spectrometry laboratory (University of Edinburgh). .. N2-01 (in its undiluted form) was diluted 40:1 in 5:3:2 methanol:acetonitrile:water and 10 ul were injected onto a ZIC-pHILIC 4.6 mm x 150 mm Sequant column (Merck, United Kingdom) on an Ultimate 3000 series HPLC (Thermo Fisher Scientific, United States) with A gradient from 90 to 5% B in 20 min, where A was 20 mm ammonium carbonate and B was acetonitrile. .. Data were acquired on Q Exactive (Thermo Fisher Scientific, United States) with HESI in positive (75.5–1132.5) and negative (77.5–1132.5) mode at 70k resolution, with ddMS2 at 17.5k.

Article Title: Development of novel MOG analogues with increased stability to explore MCT2 and α-ketoglutarate biology in vivo
Article Snippet: Liquid Chromatography-Mass Spectrometry The LC–MS method was adapted from ref . .. Samples were injected into a Dionex UltiMate LC system (Thermo Scientific) using a ZIC-pHILIC (150 mm × 4.6 mm, 5-μm particle) column (Merck Sequant). .. A 15-min elution gradient was used (80% solvent A to 20% solvent B), followed by a 5-min wash (95:5 solvent A to solvent B) and 5-min re-equilibration; solvent A was 20 mM ammonium carbonate in water (Optima HPLC grade, Sigma Aldrich) and solvent B was acetonitrile (Optima HPLC grade, Sigma Aldrich).

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    Merck & Co sequant zic philic column
    Digestion products obtained upon incubation of E. coli PGN with cell extracts of T. forsythia , prepared from cultures grown with MurNAc or PGN. Samples were taken after several time points and analysed by LC-ESI-MS in negative ion mode, showing the presence of GlcNAc-MurNAc - m/z 495.18 [M-H] − , GlcNAc-anhMurNAc - m/ z 477.17 [M-H] − , MurNAc - m/z 292.11 [M-H] − and anhMurNAc - m/z 274.09 [M-H] − . Measured masses were in accordance with the calculated masses of m/z 495.18, m/z 477.17, m/z 292.10, and m/z 274.09, respectively. Shown are the mean values of triplicates calculated from the area under the curve (AUC) ( a ). The disaccharide was confirmed to be GlcNAc-MurNAc based on the successful digestion with the N -acetylglucosaminidase NagZ, shown as the extracted ion chromatogram (EIC) upon elution from a <t>SeQuant®</t> <t>ZIC®-pHILIC</t> column, before (black) and after (red) enzyme digestion ( b )
    Sequant Zic Philic Column, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sequant zic philic column/product/Merck & Co
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sequant zic philic column - by Bioz Stars, 2021-07
    86/100 stars
      Buy from Supplier

    86
    Merck & Co zic philic column
    Digestion products obtained upon incubation of E. coli PGN with cell extracts of T. forsythia , prepared from cultures grown with MurNAc or PGN. Samples were taken after several time points and analysed by LC-ESI-MS in negative ion mode, showing the presence of GlcNAc-MurNAc - m/z 495.18 [M-H] − , GlcNAc-anhMurNAc - m/ z 477.17 [M-H] − , MurNAc - m/z 292.11 [M-H] − and anhMurNAc - m/z 274.09 [M-H] − . Measured masses were in accordance with the calculated masses of m/z 495.18, m/z 477.17, m/z 292.10, and m/z 274.09, respectively. Shown are the mean values of triplicates calculated from the area under the curve (AUC) ( a ). The disaccharide was confirmed to be GlcNAc-MurNAc based on the successful digestion with the N -acetylglucosaminidase NagZ, shown as the extracted ion chromatogram (EIC) upon elution from a <t>SeQuant®</t> <t>ZIC®-pHILIC</t> column, before (black) and after (red) enzyme digestion ( b )
    Zic Philic Column, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zic philic column/product/Merck & Co
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    zic philic column - by Bioz Stars, 2021-07
    86/100 stars
      Buy from Supplier

    86
    Merck & Co zic philic
    Digestion products obtained upon incubation of E. coli PGN with cell extracts of T. forsythia , prepared from cultures grown with MurNAc or PGN. Samples were taken after several time points and analysed by LC-ESI-MS in negative ion mode, showing the presence of GlcNAc-MurNAc - m/z 495.18 [M-H] − , GlcNAc-anhMurNAc - m/ z 477.17 [M-H] − , MurNAc - m/z 292.11 [M-H] − and anhMurNAc - m/z 274.09 [M-H] − . Measured masses were in accordance with the calculated masses of m/z 495.18, m/z 477.17, m/z 292.10, and m/z 274.09, respectively. Shown are the mean values of triplicates calculated from the area under the curve (AUC) ( a ). The disaccharide was confirmed to be GlcNAc-MurNAc based on the successful digestion with the N -acetylglucosaminidase NagZ, shown as the extracted ion chromatogram (EIC) upon elution from a <t>SeQuant®</t> <t>ZIC®-pHILIC</t> column, before (black) and after (red) enzyme digestion ( b )
    Zic Philic, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zic philic/product/Merck & Co
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    zic philic - by Bioz Stars, 2021-07
    86/100 stars
      Buy from Supplier

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    Digestion products obtained upon incubation of E. coli PGN with cell extracts of T. forsythia , prepared from cultures grown with MurNAc or PGN. Samples were taken after several time points and analysed by LC-ESI-MS in negative ion mode, showing the presence of GlcNAc-MurNAc - m/z 495.18 [M-H] − , GlcNAc-anhMurNAc - m/ z 477.17 [M-H] − , MurNAc - m/z 292.11 [M-H] − and anhMurNAc - m/z 274.09 [M-H] − . Measured masses were in accordance with the calculated masses of m/z 495.18, m/z 477.17, m/z 292.10, and m/z 274.09, respectively. Shown are the mean values of triplicates calculated from the area under the curve (AUC) ( a ). The disaccharide was confirmed to be GlcNAc-MurNAc based on the successful digestion with the N -acetylglucosaminidase NagZ, shown as the extracted ion chromatogram (EIC) upon elution from a SeQuant® ZIC®-pHILIC column, before (black) and after (red) enzyme digestion ( b )

    Journal: BMC Microbiology

    Article Title: Utilization of different MurNAc sources by the oral pathogen Tannerella forsythia and role of the inner membrane transporter AmpG

    doi: 10.1186/s12866-020-02006-z

    Figure Lengend Snippet: Digestion products obtained upon incubation of E. coli PGN with cell extracts of T. forsythia , prepared from cultures grown with MurNAc or PGN. Samples were taken after several time points and analysed by LC-ESI-MS in negative ion mode, showing the presence of GlcNAc-MurNAc - m/z 495.18 [M-H] − , GlcNAc-anhMurNAc - m/ z 477.17 [M-H] − , MurNAc - m/z 292.11 [M-H] − and anhMurNAc - m/z 274.09 [M-H] − . Measured masses were in accordance with the calculated masses of m/z 495.18, m/z 477.17, m/z 292.10, and m/z 274.09, respectively. Shown are the mean values of triplicates calculated from the area under the curve (AUC) ( a ). The disaccharide was confirmed to be GlcNAc-MurNAc based on the successful digestion with the N -acetylglucosaminidase NagZ, shown as the extracted ion chromatogram (EIC) upon elution from a SeQuant® ZIC®-pHILIC column, before (black) and after (red) enzyme digestion ( b )

    Article Snippet: Five-microliters of each sample were analysed immediately by LS-MS run in negative ion mode as described previously [ ] in order to ensure the efficient separation of GlcNAc-MurNAc and MurNAc on a SeQuant® ZIC®-pHILIC column (Merck, PEEK 150 × 2.1 mm, 5 μm).

    Techniques: Incubation