15 mer poly dt oligonucleotide  (Thermo Fisher)


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    Structured Review

    Thermo Fisher 15 mer poly dt oligonucleotide
    15 Mer Poly Dt Oligonucleotide, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/15 mer poly dt oligonucleotide/product/Thermo Fisher
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    15 mer poly dt oligonucleotide - by Bioz Stars, 2020-04
    91/100 stars

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    Related Articles

    Amplification:

    Article Title: In vivo monitoring of transfected DNA, gene expression kinetics, and cellular immune responses in mice immunized with a human NIS gene-expressing plasmid
    Article Snippet: The isolated RNAs were dissolved in diethyl pyrocarbonate-treated water (DEPC; Sigma-Aldrich, MO, USA). cDNA was generated from an mRNA template by incubating the mRNA with a 15-mer poly dT oligonucleotide (Invitrogen, CA, USA) and the Superscript reverse transcriptase enzyme (GIBCO-BRL, CA, USA) at 37°C for 1 h using the Superscript Preamplification System protocol. hNIS gene expression was detected in various organs (dLNs, non-dLNs, and spleens) by RT-PCR using the following gene-specific primers: 5’-GGCTCCTCGGTGACTCTAGGATGC-3’ (forward) and 5’-CATGAATTCTGGGCTCAATTTTCTTGTCC-3’ (reverse). .. To control for DNA integrity, the mouse β-actin gene (codons 135-223) was amplified using the following gene-specific primers: 5’-GGCTCCTCGGTGACTCTAGGATGC-3’ (forward) and 5’-CATGAATTCTGGGCTCAATTTTCTTGTCC-3’ (reverse).

    Isolation:

    Article Title: In vivo monitoring of transfected DNA, gene expression kinetics, and cellular immune responses in mice immunized with a human NIS gene-expressing plasmid
    Article Snippet: .. The isolated RNAs were dissolved in diethyl pyrocarbonate-treated water (DEPC; Sigma-Aldrich, MO, USA). cDNA was generated from an mRNA template by incubating the mRNA with a 15-mer poly dT oligonucleotide (Invitrogen, CA, USA) and the Superscript reverse transcriptase enzyme (GIBCO-BRL, CA, USA) at 37°C for 1 h using the Superscript Preamplification System protocol. hNIS gene expression was detected in various organs (dLNs, non-dLNs, and spleens) by RT-PCR using the following gene-specific primers: 5’-GGCTCCTCGGTGACTCTAGGATGC-3’ (forward) and 5’-CATGAATTCTGGGCTCAATTTTCTTGTCC-3’ (reverse). .. To control for DNA integrity, the mouse β-actin gene (codons 135-223) was amplified using the following gene-specific primers: 5’-GGCTCCTCGGTGACTCTAGGATGC-3’ (forward) and 5’-CATGAATTCTGGGCTCAATTTTCTTGTCC-3’ (reverse).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: In vivo monitoring of transfected DNA, gene expression kinetics, and cellular immune responses in mice immunized with a human NIS gene-expressing plasmid
    Article Snippet: .. The isolated RNAs were dissolved in diethyl pyrocarbonate-treated water (DEPC; Sigma-Aldrich, MO, USA). cDNA was generated from an mRNA template by incubating the mRNA with a 15-mer poly dT oligonucleotide (Invitrogen, CA, USA) and the Superscript reverse transcriptase enzyme (GIBCO-BRL, CA, USA) at 37°C for 1 h using the Superscript Preamplification System protocol. hNIS gene expression was detected in various organs (dLNs, non-dLNs, and spleens) by RT-PCR using the following gene-specific primers: 5’-GGCTCCTCGGTGACTCTAGGATGC-3’ (forward) and 5’-CATGAATTCTGGGCTCAATTTTCTTGTCC-3’ (reverse). .. To control for DNA integrity, the mouse β-actin gene (codons 135-223) was amplified using the following gene-specific primers: 5’-GGCTCCTCGGTGACTCTAGGATGC-3’ (forward) and 5’-CATGAATTCTGGGCTCAATTTTCTTGTCC-3’ (reverse).

    Generated:

    Article Title: In vivo monitoring of transfected DNA, gene expression kinetics, and cellular immune responses in mice immunized with a human NIS gene-expressing plasmid
    Article Snippet: .. The isolated RNAs were dissolved in diethyl pyrocarbonate-treated water (DEPC; Sigma-Aldrich, MO, USA). cDNA was generated from an mRNA template by incubating the mRNA with a 15-mer poly dT oligonucleotide (Invitrogen, CA, USA) and the Superscript reverse transcriptase enzyme (GIBCO-BRL, CA, USA) at 37°C for 1 h using the Superscript Preamplification System protocol. hNIS gene expression was detected in various organs (dLNs, non-dLNs, and spleens) by RT-PCR using the following gene-specific primers: 5’-GGCTCCTCGGTGACTCTAGGATGC-3’ (forward) and 5’-CATGAATTCTGGGCTCAATTTTCTTGTCC-3’ (reverse). .. To control for DNA integrity, the mouse β-actin gene (codons 135-223) was amplified using the following gene-specific primers: 5’-GGCTCCTCGGTGACTCTAGGATGC-3’ (forward) and 5’-CATGAATTCTGGGCTCAATTTTCTTGTCC-3’ (reverse).

    Expressing:

    Article Title: In vivo monitoring of transfected DNA, gene expression kinetics, and cellular immune responses in mice immunized with a human NIS gene-expressing plasmid
    Article Snippet: .. The isolated RNAs were dissolved in diethyl pyrocarbonate-treated water (DEPC; Sigma-Aldrich, MO, USA). cDNA was generated from an mRNA template by incubating the mRNA with a 15-mer poly dT oligonucleotide (Invitrogen, CA, USA) and the Superscript reverse transcriptase enzyme (GIBCO-BRL, CA, USA) at 37°C for 1 h using the Superscript Preamplification System protocol. hNIS gene expression was detected in various organs (dLNs, non-dLNs, and spleens) by RT-PCR using the following gene-specific primers: 5’-GGCTCCTCGGTGACTCTAGGATGC-3’ (forward) and 5’-CATGAATTCTGGGCTCAATTTTCTTGTCC-3’ (reverse). .. To control for DNA integrity, the mouse β-actin gene (codons 135-223) was amplified using the following gene-specific primers: 5’-GGCTCCTCGGTGACTCTAGGATGC-3’ (forward) and 5’-CATGAATTCTGGGCTCAATTTTCTTGTCC-3’ (reverse).

    Polymerase Chain Reaction:

    Article Title: In vivo monitoring of transfected DNA, gene expression kinetics, and cellular immune responses in mice immunized with a human NIS gene-expressing plasmid
    Article Snippet: The isolated RNAs were dissolved in diethyl pyrocarbonate-treated water (DEPC; Sigma-Aldrich, MO, USA). cDNA was generated from an mRNA template by incubating the mRNA with a 15-mer poly dT oligonucleotide (Invitrogen, CA, USA) and the Superscript reverse transcriptase enzyme (GIBCO-BRL, CA, USA) at 37°C for 1 h using the Superscript Preamplification System protocol. hNIS gene expression was detected in various organs (dLNs, non-dLNs, and spleens) by RT-PCR using the following gene-specific primers: 5’-GGCTCCTCGGTGACTCTAGGATGC-3’ (forward) and 5’-CATGAATTCTGGGCTCAATTTTCTTGTCC-3’ (reverse). .. For amplification of the β-actin gene, the following PCR conditions were used: 34 cycles of 60 s at 94°C, 60 s at 55°C, and 60 s at 72°C.

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