Review




Structured Review

Tosoh Corporation deae toyopearl 650
Deae Toyopearl 650, supplied by Tosoh Corporation, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/14693/pm39772954-43-18-22
Average 95 stars, based on 1 article reviews
deae toyopearl 650 - by Bioz Stars, 2026-09
95/100 stars

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Incubation:

Article Title: Molecular interactions of photosystem I and ZIF-8 in bio-nanohybrid materials.
Article Snippet: .. The samples were gently mixed by pipetting and incubated on ice for 30 min. Insoluble materials were removed by ultracentrifugation at 40 000 rpm for 1 h at 4 1C, and the supernatant was collected and applied to an ion-exchange column (Toyopearl DEAE-650M, 5 mL, TOSOH BIOSCIENCEs) on Äkta. ..

Chromatography:

Article Title: Structure and Biosynthetic Gene Cluster of Sulfated Capsular Polysaccharide from the Marine Bacterium Vibrio sp. KMM 8419
Article Snippet: .. The freeze-dried supernatant was subjected to anion exchange chromatography on a column (10 × 1.5 cm) of Toyopearl DEAE-650M (Tosoh Bioscience, Tokyo, Japan) in a step-wise gradient of NaCl. ..

Article Title: Structure and Biosynthetic Gene Cluster of Sulfated Capsular Polysaccharide from the Marine Bacterium Vibrio sp. KMM 8419.
Article Snippet: .. The freeze-dried supernatant was subjected to anion exchange chromatography on a column (10 × 1.5 cm) of Toyopearl DEAE-650M (Tosoh Bioscience, Tokyo, Japan) in a step-wise gradient of NaCl. ..

Article Title: Structural characterization of pyruvic oxime dioxygenase, a key enzyme of heterotrophic nitrification
Article Snippet: .. AfPOD was overexpressed in the Escherichia coli cells and purified by two chromatographic steps: anion-exchange chromatography on DEAE-Toyopearl 650 M gel (Tosoh, Tokyo, Japan) and gel filtration chromatography on Sephacryl S-200 (Cytiva, Marlborough, MA), as described previously ( ). ..

Article Title: Structural characterization of pyruvic oxime dioxygenase, a key enzyme in heterotrophic nitrification.
Article Snippet: .. AfPOD was overexpressed in the Escherichia coli cells and purified by two chromato graphic steps: anion-exchange chromatography on DEAE-Toyopearl 650 M gel (Tosoh, Tokyo, Japan) and gel filtration chromatography on Sephacryl S-200 (Cytiva, Marlbor ough, MA, USA), as described previously (9). ..

other:

Article Title: Gene cloning and characterization of N-carbamyl-l-glutamic acid amidohydrolase involved in ergothioneine utilization in Burkholderia sp. HME13.
Article Snippet: Toyopearl 18 Butyl-650M and DEAE-650M were purchased from Tosoh (Tokyo, Japan).

Membrane:

Article Title: Use of alkaline washes during chromatography to remove impurities
Article Snippet: Commercially available anion exchange resins include DEAE cellulose, POROS® PI 20, P150, HQ 10, HQ 20, HQ 50, D 50 from Applied Biosystems, SARTOBIND® Q from Sartorius, MonoQ, MiniQ, Source 15Q and 30Q, Q, DEAE and ANX SEPHAROSE® Fast Flow, Q SEPHAROSE® High Performance, QAE SEPHADEX® and FAST Q SEPHAROSE® (GE Healthcare), WP PEI, WP DEAM, WP QUAT from J.T. .. Baker, Hydrocell DEAE and Hydrocell QA from Biochrom Labs Inc., UNOsphere Q, MACRO-PREP® DEAE and MACRO-PREP® High Q from Biorad, Ceramic HyperD Q, ceramic HyperD DEAE, TRISACRYL® M and LS DEAE, Spherodex LS DEAE, QMA SPHEROSIL® LS, QMA SPHEROSIL® M and MUSTANG® Q from Pall Technologies, DOWEX® Fine Mesh Strong Base Type I and Type II Anion Resins and DOWEX® MONOSPHER E 77, weak base anion from Dow Liquid Separations, INTERCEPT® Q membrane, Matrex CELLUFINE® A200, A500, Q500, and Q800, from Millipore, FRACTOGEL® EMD TMAE, FRACTOGEL® EMD DEAE and FRACTOGEL® EMD DMAE from EMD, AMBERLITE® weak strong anion exchangers type I and II, DOWEX® weak and strong anion exchangers type I and II, DIAION® weak and strong anion exchangers type I and II, DUOLITE® from Sigma-Aldrich, TSK gel Q and DEAE 5PW and 5PW-HR, TOYOPEARL® SuperQ-650S, 650M and 650C, QAE-550C and 650S, DEAE-650M and 650C from Tosoh, QA52, DE23, DE32, DE51, DE52, DE53, Express-Ion D and Express-ion Q from Whatman, and SARTOBIND® Q (Sartorius Corporation, New York, USA). .. A “mixed mode ion exchange resin”, “mixed mode ion exchange membrane” or “mixed mode” refers to a solid phase which is covalently modified with cationic, anionic, and/or hydrophobic moieties.

Purification:

Article Title: Structural characterization of pyruvic oxime dioxygenase, a key enzyme of heterotrophic nitrification
Article Snippet: .. AfPOD was overexpressed in the Escherichia coli cells and purified by two chromatographic steps: anion-exchange chromatography on DEAE-Toyopearl 650 M gel (Tosoh, Tokyo, Japan) and gel filtration chromatography on Sephacryl S-200 (Cytiva, Marlborough, MA), as described previously ( ). ..

Article Title: Structural characterization of pyruvic oxime dioxygenase, a key enzyme in heterotrophic nitrification.
Article Snippet: .. AfPOD was overexpressed in the Escherichia coli cells and purified by two chromato graphic steps: anion-exchange chromatography on DEAE-Toyopearl 650 M gel (Tosoh, Tokyo, Japan) and gel filtration chromatography on Sephacryl S-200 (Cytiva, Marlbor ough, MA, USA), as described previously (9). ..

Filtration:

Article Title: Structural characterization of pyruvic oxime dioxygenase, a key enzyme of heterotrophic nitrification
Article Snippet: .. AfPOD was overexpressed in the Escherichia coli cells and purified by two chromatographic steps: anion-exchange chromatography on DEAE-Toyopearl 650 M gel (Tosoh, Tokyo, Japan) and gel filtration chromatography on Sephacryl S-200 (Cytiva, Marlborough, MA), as described previously ( ). ..

Article Title: Structural characterization of pyruvic oxime dioxygenase, a key enzyme in heterotrophic nitrification.
Article Snippet: .. AfPOD was overexpressed in the Escherichia coli cells and purified by two chromato graphic steps: anion-exchange chromatography on DEAE-Toyopearl 650 M gel (Tosoh, Tokyo, Japan) and gel filtration chromatography on Sephacryl S-200 (Cytiva, Marlbor ough, MA, USA), as described previously (9). ..



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