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ptpn22  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc ptpn22
    Ptpn22, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/14693/PTPN22+Rabbit+mAb/pm41318084-77-2-33
    Average 94 stars, based on 24 article reviews
    ptpn22 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    other:

    Article Title: The Tyrosine Phosphatase Activity of PTPN22 Is Involved in T Cell Development via the Regulation of TCR Expression.
    Article Snippet: Antibodies including anti‐PTPN22 (#14693), anti‐PLCγ1 (#5690), anti‐GAPDH (#2118), anti‐phospho‐PLCγ1 (Y783) (#2821), anti‐pErk1 (Thr202)/Erk2 (Tyr204) (#04370), anti‐CD3ε (#4443) and anti‐rat IgG antibody coupled to Alexa Fluor 488 (#4416) were purchased from Cell Signaling Technology (CST, Danvers, MA, USA).

    Immunoprecipitation:

    Article Title: Phosphatase PTPN22 functions as an adaptor in the mTORC2 complex
    Article Snippet: .. Immunoprecipitation (IP) and western blotting (WB) were performed by using the following antibodies: PTPN22 (CST 14693S; WB 1:1000 or IP 1:100 or 1:1000), mTOR (CST 2972S; WB 1:1000), mTOR (CST 2983S; WB 1:1000), RICTOR (CST 9476S; WB 1:1000), RAPTOR (CST 2280S; WB 1:1000), mLST8/GβL (CST 3274S; WB 1:1000), SIN1 (abcam ab71152; WB 1:3000 or IP 2.5 μg), AKT (CST 9272S; WB 1:2000), pS473-AKT (CST 9271L; WB 1:1500), pS473-AKT (CST 9018S; WB 1:1500), pT308-AKT (CST 9275L; WB 1:1000), GSK-3β (CST 12456T; WB 1:2000), pS9-GSK-3β (CST 5558T; WB 1:1000), FoxO1 (CST 2880T; WB 1:1000), pT24-FoxO1/pT32-FoxO3a (CST 9464T; WB 1:1000), p70S6K (CST 2708S; WB 1:2000), pT389-p70S6K (CST 9205S; WB 1:1000), PKCα (CST 2056T; WB 1:1000), phospho-PKCα/βII [pT638/641] (CST 9375T; WB 1:1000), SGK1 (ab59337; WB 1:1000), pS422-SGK1 (ab55281; WB 1:500), pS10-Histone H3 (CST 9701L; IF 1:200), β-actin (Sigma-Aldrich A5441; WB 1:10,000), FLAG (Sigma-Aldrich F3165; WB 1:10,000 or IP 2 μg), c-Myc (Santa Cruz Biotechnologies clone 9E10 (sc-40); WB 1:1000), c-Myc (Sigma-Aldrich M4439; IP 1 μg), MBP (Sigma-Aldrich M1321; WB 1:5000), anti-6-His (Bethyl A190-114A; 1:800) and GST (Santa Cruz Biotechnologies sc-138; WB 1:2500). .. Secondary antibodies: HRP-conjugated anti-mouse (315-035-048) and anti-rabbit (111-035-144) antibodies were obtained from Jackson Immunologicals.

    Western Blot:

    Article Title: Phosphatase PTPN22 functions as an adaptor in the mTORC2 complex
    Article Snippet: .. Immunoprecipitation (IP) and western blotting (WB) were performed by using the following antibodies: PTPN22 (CST 14693S; WB 1:1000 or IP 1:100 or 1:1000), mTOR (CST 2972S; WB 1:1000), mTOR (CST 2983S; WB 1:1000), RICTOR (CST 9476S; WB 1:1000), RAPTOR (CST 2280S; WB 1:1000), mLST8/GβL (CST 3274S; WB 1:1000), SIN1 (abcam ab71152; WB 1:3000 or IP 2.5 μg), AKT (CST 9272S; WB 1:2000), pS473-AKT (CST 9271L; WB 1:1500), pS473-AKT (CST 9018S; WB 1:1500), pT308-AKT (CST 9275L; WB 1:1000), GSK-3β (CST 12456T; WB 1:2000), pS9-GSK-3β (CST 5558T; WB 1:1000), FoxO1 (CST 2880T; WB 1:1000), pT24-FoxO1/pT32-FoxO3a (CST 9464T; WB 1:1000), p70S6K (CST 2708S; WB 1:2000), pT389-p70S6K (CST 9205S; WB 1:1000), PKCα (CST 2056T; WB 1:1000), phospho-PKCα/βII [pT638/641] (CST 9375T; WB 1:1000), SGK1 (ab59337; WB 1:1000), pS422-SGK1 (ab55281; WB 1:500), pS10-Histone H3 (CST 9701L; IF 1:200), β-actin (Sigma-Aldrich A5441; WB 1:10,000), FLAG (Sigma-Aldrich F3165; WB 1:10,000 or IP 2 μg), c-Myc (Santa Cruz Biotechnologies clone 9E10 (sc-40); WB 1:1000), c-Myc (Sigma-Aldrich M4439; IP 1 μg), MBP (Sigma-Aldrich M1321; WB 1:5000), anti-6-His (Bethyl A190-114A; 1:800) and GST (Santa Cruz Biotechnologies sc-138; WB 1:2500). .. Secondary antibodies: HRP-conjugated anti-mouse (315-035-048) and anti-rabbit (111-035-144) antibodies were obtained from Jackson Immunologicals.

    Concentration Assay:

    Article Title: Angulin-1/LSR inhibition transiently disrupts the blood-tumor barrier to enhance doxil permeability and impair malignant glioma progression
    Article Snippet: 1:1000 dilution of primary antibodies Occludin (#ABIN500409), Claudin-5 (#ABIN6260869), ZO-1 (#ABIN675024), Tricellulin/MARVELD2 (#ABIN7264716), and Angulin-1/LSR (# ABIN6737870) were used and purchased from Antibodies Online (Limerick, PA, USA). .. Vinculin mAb (#4650), GAPDH mAb (#2118), HA-tag rabbit mAb (#3724), or DYKDDDDK Tag (D6W5B) Rabbit mAb (#14693) from Cell Signaling (Danvers, MA, USA) were diluted to 1:1000 concentration. ..



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