Journal: bioRxiv

Article Title: Discovery of novel microRNA regulators of ribosome biogenesis

doi: 10.1101/2023.02.17.526327

Figure Lengend Snippet: ( A ). Plot of genes with mRNA transcripts targeted by both hsa-miR-28-5p and hsa-miR-708-5p. The miR-eCLIP data were filtered for genes expressed in MCF10A cells, and RNAseq differential expression data following MIR-28 treatment is graphed for each gene on the x- and y-axes. Bolded points represent genes downregulated by both MIR-28 mimics by at least -0.5 log2 units, with a differential expression false discovery rate < 0.05 for both treatments. All genes are labeled with their HGNC symbol. ( B ). Plot of genes with mRNA transcripts targeted exclusively (XOR) by either hsa-miR-28-5p or hsa- miR-708-5p. The miR-eCLIP data were filtered and graphed as above. The genes encoding mRNA transcripts are colored blue if bound by hsa-miR-28-5p or red if bound by hsa-miR-708-5p, according to miR-eCLIP data. Select genes are labeled with HGNC symbol.

Article Snippet: Approximately 10 million crosslinked cells from each microRNA mimic treatment were pooled and processed for miR-eCLIP sequencing and data analysis (Eclipse Bioinnovations, San Diego, CA).

Techniques: Expressing, Labeling