Microarray:Article Title: Multiplex microarray of serially deposited biomolecules on a microarray
Article Snippet: .. Serial Attachment of Proteins on 12K CombiMatrix Microarray In an embodiment, a method for serial construction of a protein microarray comprises: (a) blocking a microarray with a blocking protein, wherein the blocking protein is Casein or Bovine Calf Serum or a combination thereof; (b) washing the microarray to remove the blocking solution, wherein the step of washing comprises washing three times with PBS/Tween, three times with PBS, and three times with the electrolyte used for the polymerization of pyrrole; (c) electropolymerizing pyrrole for 5 seconds at 1.5 V in Sodium Phosphate on an electrode or set of electrodes that have been predetermined; (d) washing the microarray 3 times with PBS; (e) diluting a protein solution to about 1 ug/ml; (f) exposing the microarray to the protein solution for about 5 to 30 minutes; (g) washing the microarray three times with PBS/tween; (h) blocking the microarray with a blocking solution, wherein the blocking solution is saturated Casein and the time of blocking is about 2-5 minutes; and (i) repeating steps (b) through (h) for each protein to be attached to the microarray. ..
Article Title: Microarray analysis of differentially expressed mRNAs and miRNAs in young leaves of sorghum under dry-down conditions.
Article Snippet: Sorghum is a C4 plant adapted to semi-arid environments, and characterized by high water-use efficiency.. To better understand the molecular and physiological basis of drought response the sorghum genotype IS19453, selected as a drought tolerant line during field trials, was evaluated in a “dry-down” experiment under controlled conditions.. The incoming stress was monitored by determining the water potential available for 4-leaf-old plants.
Article Title: Serially deposited biomolecules
Article Snippet: .. Serial Attachment of Proteins on 12K CombiMatrix Microarray In an embodiment, a method for serial construction of a protein microarray comprises: (a) blocking a microarray with a blocking protein, wherein the blocking protein is Casein or Bovine Calf Serum or a combination thereof; (b) washing the microarray to remove the blocking solution, wherein the step of washing comprises washing three times with PBS/Tween, three times with PBS, and three times with the electrolyte used for the polymerization of pyrrole; (c) electropolymerizing pyrrole for 5 seconds at 1.5 V in Sodium Phosphate on an electrode or set of electrodes that have been predetermined; (d) washing the microarray 3 times with PBS; (e) diluting a protein solution to about 1 μg/ml; (f) exposing the microarray to the protein solution for about 5 to 30 minutes; (g) washing the microarray three times with PBS/tween; (h) blocking the microarray with a blocking solution, wherein the blocking solution is saturated Casein and the time of blocking is about 2-5 minutes; and (i) repeating steps (b) through (h) for each protein to be attached to the microarray. ..
Blocking Assay:Article Title: Multiplex microarray of serially deposited biomolecules on a microarray
Article Snippet: .. Serial Attachment of Proteins on 12K CombiMatrix Microarray In an embodiment, a method for serial construction of a protein microarray comprises: (a) blocking a microarray with a blocking protein, wherein the blocking protein is Casein or Bovine Calf Serum or a combination thereof; (b) washing the microarray to remove the blocking solution, wherein the step of washing comprises washing three times with PBS/Tween, three times with PBS, and three times with the electrolyte used for the polymerization of pyrrole; (c) electropolymerizing pyrrole for 5 seconds at 1.5 V in Sodium Phosphate on an electrode or set of electrodes that have been predetermined; (d) washing the microarray 3 times with PBS; (e) diluting a protein solution to about 1 ug/ml; (f) exposing the microarray to the protein solution for about 5 to 30 minutes; (g) washing the microarray three times with PBS/tween; (h) blocking the microarray with a blocking solution, wherein the blocking solution is saturated Casein and the time of blocking is about 2-5 minutes; and (i) repeating steps (b) through (h) for each protein to be attached to the microarray. ..
Article Title: Serially deposited biomolecules
Article Snippet: .. Serial Attachment of Proteins on 12K CombiMatrix Microarray In an embodiment, a method for serial construction of a protein microarray comprises: (a) blocking a microarray with a blocking protein, wherein the blocking protein is Casein or Bovine Calf Serum or a combination thereof; (b) washing the microarray to remove the blocking solution, wherein the step of washing comprises washing three times with PBS/Tween, three times with PBS, and three times with the electrolyte used for the polymerization of pyrrole; (c) electropolymerizing pyrrole for 5 seconds at 1.5 V in Sodium Phosphate on an electrode or set of electrodes that have been predetermined; (d) washing the microarray 3 times with PBS; (e) diluting a protein solution to about 1 μg/ml; (f) exposing the microarray to the protein solution for about 5 to 30 minutes; (g) washing the microarray three times with PBS/tween; (h) blocking the microarray with a blocking solution, wherein the blocking solution is saturated Casein and the time of blocking is about 2-5 minutes; and (i) repeating steps (b) through (h) for each protein to be attached to the microarray. ..
Expressing:Article Title: Microarray analysis of differentially expressed mRNAs and miRNAs in young leaves of sorghum under dry-down conditions.
Article Snippet: Sorghum is a C4 plant adapted to semi-arid environments, and characterized by high water-use efficiency.. To better understand the molecular and physiological basis of drought response the sorghum genotype IS19453, selected as a drought tolerant line during field trials, was evaluated in a “dry-down” experiment under controlled conditions.. The incoming stress was monitored by determining the water potential available for 4-leaf-old plants.
Hybridization:Article Title: Microarray analysis of differentially expressed mRNAs and miRNAs in young leaves of sorghum under dry-down conditions.
Article Snippet: Sorghum is a C4 plant adapted to semi-arid environments, and characterized by high water-use efficiency.. To better understand the molecular and physiological basis of drought response the sorghum genotype IS19453, selected as a drought tolerant line during field trials, was evaluated in a “dry-down” experiment under controlled conditions.. The incoming stress was monitored by determining the water potential available for 4-leaf-old plants.
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