powersoil dna isolation kit  (Qiagen)

 
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    Name:
    DNeasy PowerSoil Kit
    Description:
    For the isolation of microbial genomic DNA from all soil types Kit contents For the isolation of microbial genomic DNA from all soil types Benefits Fast and easy protocol isolates high quality DNA from up to 250 mg samples in just 30 minutes Inhibitor Removal Technology removes PCR inhibitors found in tough sample types Optimized to isolate DNA from difficult even the most difficult environmental samp
    Catalog Number:
    12888-100
    Price:
    550
    Category:
    DNeasy PowerSoil Kit
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    Structured Review

    Qiagen powersoil dna isolation kit
    DNeasy PowerSoil Kit
    For the isolation of microbial genomic DNA from all soil types Kit contents For the isolation of microbial genomic DNA from all soil types Benefits Fast and easy protocol isolates high quality DNA from up to 250 mg samples in just 30 minutes Inhibitor Removal Technology removes PCR inhibitors found in tough sample types Optimized to isolate DNA from difficult even the most difficult environmental samp
    https://www.bioz.com/result/powersoil dna isolation kit/product/Qiagen
    Average 99 stars, based on 19125 article reviews
    Price from $9.99 to $1999.99
    powersoil dna isolation kit - by Bioz Stars, 2020-07
    99/100 stars

    Images

    1) Product Images from "Comparing Two Common DNA Extraction Kits for the Characterization of Symbiotic Microbial Communities from Ascidian Tissue"

    Article Title: Comparing Two Common DNA Extraction Kits for the Characterization of Symbiotic Microbial Communities from Ascidian Tissue

    Journal: Microbes and Environments

    doi: 10.1264/jsme2.ME18031

    Non-metric multi-dimensional scaling plot based on the Bray-Curtis similarity of microbial communities obtained using DNeasy (black triangles) and PowerSoil (gray circles) kits. Circles encompass samples clustering at > 30% similarity and correspond to replicates of Clavelina oblonga (dashed line) and Polyandrocarpa anguinea (solid line), indicating a high degree of host-specificity and low technical variation across DNA extraction methods.
    Figure Legend Snippet: Non-metric multi-dimensional scaling plot based on the Bray-Curtis similarity of microbial communities obtained using DNeasy (black triangles) and PowerSoil (gray circles) kits. Circles encompass samples clustering at > 30% similarity and correspond to replicates of Clavelina oblonga (dashed line) and Polyandrocarpa anguinea (solid line), indicating a high degree of host-specificity and low technical variation across DNA extraction methods.

    Techniques Used: DNA Extraction

    2) Product Images from "16S rRNA gene pyrosequencing of reference and clinical samples and investigation of the temperature stability of microbiome profiles"

    Article Title: 16S rRNA gene pyrosequencing of reference and clinical samples and investigation of the temperature stability of microbiome profiles

    Journal: Microbiome

    doi: 10.1186/2049-2618-2-31

    Normalization of PCR amplification by choosing PCR cycle number close to the Ct value. Throat swabs from healthy volunteers were extracted with Qiagen DNeasy (D) or MO BIO PowerSoil (M). DNA extracts were subjected to 16S gene TaqMan qPCR and subsequent PCR using a cycle number of 20, 25, or 30 based on individual sample's qPCR Ct value. PCR amplicons were quantified by PicoGreen dsDNA assay. DNA concentrations for DNA extracts ( blue markers and axis ) and PCR amplicons ( red markers and axis ) are shown in 16S gene copies/μl in the same scale. DNA sample name, 08S1M as an example, depicts the subject number (08), swab number (S1), and extraction method (M).
    Figure Legend Snippet: Normalization of PCR amplification by choosing PCR cycle number close to the Ct value. Throat swabs from healthy volunteers were extracted with Qiagen DNeasy (D) or MO BIO PowerSoil (M). DNA extracts were subjected to 16S gene TaqMan qPCR and subsequent PCR using a cycle number of 20, 25, or 30 based on individual sample's qPCR Ct value. PCR amplicons were quantified by PicoGreen dsDNA assay. DNA concentrations for DNA extracts ( blue markers and axis ) and PCR amplicons ( red markers and axis ) are shown in 16S gene copies/μl in the same scale. DNA sample name, 08S1M as an example, depicts the subject number (08), swab number (S1), and extraction method (M).

    Techniques Used: Polymerase Chain Reaction, Amplification, Real-time Polymerase Chain Reaction, Picogreen Assay

    Storage temperature comparisons for Qiagen DNeasy and MO BIO PowerSoil DNA extraction methods for the mock bacterial community HM-280. Identical samples from the mock community were stored at four different temperatures and extracted after 4 weeks using the two methods, Qiagen DNeasy and MO BIO PowerSoil. Microbial profiles at the genus level were estimated using QIIME. Overall, samples were well preserved at lower temperatures for both methods, whereas significant differences were observed at 37°C.
    Figure Legend Snippet: Storage temperature comparisons for Qiagen DNeasy and MO BIO PowerSoil DNA extraction methods for the mock bacterial community HM-280. Identical samples from the mock community were stored at four different temperatures and extracted after 4 weeks using the two methods, Qiagen DNeasy and MO BIO PowerSoil. Microbial profiles at the genus level were estimated using QIIME. Overall, samples were well preserved at lower temperatures for both methods, whereas significant differences were observed at 37°C.

    Techniques Used: DNA Extraction

    3) Product Images from "Comparing Two Common DNA Extraction Kits for the Characterization of Symbiotic Microbial Communities from Ascidian Tissue"

    Article Title: Comparing Two Common DNA Extraction Kits for the Characterization of Symbiotic Microbial Communities from Ascidian Tissue

    Journal: Microbes and Environments

    doi: 10.1264/jsme2.ME18031

    Non-metric multi-dimensional scaling plot based on the Bray-Curtis similarity of microbial communities obtained using DNeasy (black triangles) and PowerSoil (gray circles) kits. Circles encompass samples clustering at > 30% similarity and correspond to replicates of Clavelina oblonga (dashed line) and Polyandrocarpa anguinea (solid line), indicating a high degree of host-specificity and low technical variation across DNA extraction methods.
    Figure Legend Snippet: Non-metric multi-dimensional scaling plot based on the Bray-Curtis similarity of microbial communities obtained using DNeasy (black triangles) and PowerSoil (gray circles) kits. Circles encompass samples clustering at > 30% similarity and correspond to replicates of Clavelina oblonga (dashed line) and Polyandrocarpa anguinea (solid line), indicating a high degree of host-specificity and low technical variation across DNA extraction methods.

    Techniques Used: DNA Extraction

    4) Product Images from "Comparing Two Common DNA Extraction Kits for the Characterization of Symbiotic Microbial Communities from Ascidian Tissue"

    Article Title: Comparing Two Common DNA Extraction Kits for the Characterization of Symbiotic Microbial Communities from Ascidian Tissue

    Journal: Microbes and Environments

    doi: 10.1264/jsme2.ME18031

    Non-metric multi-dimensional scaling plot based on the Bray-Curtis similarity of microbial communities obtained using DNeasy (black triangles) and PowerSoil (gray circles) kits. Circles encompass samples clustering at > 30% similarity and correspond to replicates of Clavelina oblonga (dashed line) and Polyandrocarpa anguinea (solid line), indicating a high degree of host-specificity and low technical variation across DNA extraction methods.
    Figure Legend Snippet: Non-metric multi-dimensional scaling plot based on the Bray-Curtis similarity of microbial communities obtained using DNeasy (black triangles) and PowerSoil (gray circles) kits. Circles encompass samples clustering at > 30% similarity and correspond to replicates of Clavelina oblonga (dashed line) and Polyandrocarpa anguinea (solid line), indicating a high degree of host-specificity and low technical variation across DNA extraction methods.

    Techniques Used: DNA Extraction

    5) Product Images from "Comparing Two Common DNA Extraction Kits for the Characterization of Symbiotic Microbial Communities from Ascidian Tissue"

    Article Title: Comparing Two Common DNA Extraction Kits for the Characterization of Symbiotic Microbial Communities from Ascidian Tissue

    Journal: Microbes and Environments

    doi: 10.1264/jsme2.ME18031

    Non-metric multi-dimensional scaling plot based on the Bray-Curtis similarity of microbial communities obtained using DNeasy (black triangles) and PowerSoil (gray circles) kits. Circles encompass samples clustering at > 30% similarity and correspond to replicates of Clavelina oblonga (dashed line) and Polyandrocarpa anguinea (solid line), indicating a high degree of host-specificity and low technical variation across DNA extraction methods.
    Figure Legend Snippet: Non-metric multi-dimensional scaling plot based on the Bray-Curtis similarity of microbial communities obtained using DNeasy (black triangles) and PowerSoil (gray circles) kits. Circles encompass samples clustering at > 30% similarity and correspond to replicates of Clavelina oblonga (dashed line) and Polyandrocarpa anguinea (solid line), indicating a high degree of host-specificity and low technical variation across DNA extraction methods.

    Techniques Used: DNA Extraction

    Related Articles

    Sequencing:

    Article Title: Quantification of Mercury Bioavailability for Methylation Using Diffusive Gradient in Thin-Film Samplers
    Article Snippet: .. Microbial community abundance and diversity was assessed by analysis of genomic DNA (gDNA) via 16S rRNA SSU gene sequencing, and the Hg-methylating community was quantified via PCR-based methods (qualitative broad-range, hgcAB ; quantitative clade-specific, hgcA ), as described previously. gDNA was extracted following the DNeasy PowerSoil Kit with minor modifications (Qiagen Inc., Germantown, MD). .. Final nucleic acid concentrations and purity were measured with a Qubit (Thermo Fisher Scientific) and a NanoDrop One (Thermo Fisher Scientific), respectively.

    Article Title: Comparing Two Common DNA Extraction Kits for the Characterization of Symbiotic Microbial Communities from Ascidian Tissue
    Article Snippet: .. The microbiomes of two ascidian species were herein characterized using two common DNA extraction kits, the DNeasy Blood and Tissue Kit (Qiagen) and the PowerSoil DNA Isolation Kit (Mo Bio Laboratories), followed by next-generation (Illumina) sequencing of partial 16S rRNA genes. ..

    Polymerase Chain Reaction:

    Article Title: Quantification of Mercury Bioavailability for Methylation Using Diffusive Gradient in Thin-Film Samplers
    Article Snippet: .. Microbial community abundance and diversity was assessed by analysis of genomic DNA (gDNA) via 16S rRNA SSU gene sequencing, and the Hg-methylating community was quantified via PCR-based methods (qualitative broad-range, hgcAB ; quantitative clade-specific, hgcA ), as described previously. gDNA was extracted following the DNeasy PowerSoil Kit with minor modifications (Qiagen Inc., Germantown, MD). .. Final nucleic acid concentrations and purity were measured with a Qubit (Thermo Fisher Scientific) and a NanoDrop One (Thermo Fisher Scientific), respectively.

    Incubation:

    Article Title: 16S rRNA gene pyrosequencing of reference and clinical samples and investigation of the temperature stability of microbiome profiles
    Article Snippet: .. Triplicate Copan flocked swabs spiked with microbial mock community A cells (BEI HM-280) were incubated at 37°C, 4°C, -20°C, or -80°C for 4 weeks, then extracted with MO BIO PowerSoil DNA Isolation Kit or Qiagen DNeasy Blood & Tissue Kit with enzymatic treatment. .. TaqMan quantitative PCR was used to determine the 16S rDNA copy number in the DNA extracts.

    DNA Extraction:

    Article Title: Comparing Two Common DNA Extraction Kits for the Characterization of Symbiotic Microbial Communities from Ascidian Tissue
    Article Snippet: .. The microbiomes of two ascidian species were herein characterized using two common DNA extraction kits, the DNeasy Blood and Tissue Kit (Qiagen) and the PowerSoil DNA Isolation Kit (Mo Bio Laboratories), followed by next-generation (Illumina) sequencing of partial 16S rRNA genes. ..

    Article Title: 16S rRNA gene pyrosequencing of reference and clinical samples and investigation of the temperature stability of microbiome profiles
    Article Snippet: .. Triplicate Copan flocked swabs spiked with microbial mock community A cells (BEI HM-280) were incubated at 37°C, 4°C, -20°C, or -80°C for 4 weeks, then extracted with MO BIO PowerSoil DNA Isolation Kit or Qiagen DNeasy Blood & Tissue Kit with enzymatic treatment. .. TaqMan quantitative PCR was used to determine the 16S rDNA copy number in the DNA extracts.

    Article Title: Comparing Two Common DNA Extraction Kits for the Characterization of Symbiotic Microbial Communities from Ascidian Tissue
    Article Snippet: .. To compare the efficacy of the two DNA extraction kits, tunic tissue from each replicate was processed separately using the DNeasy Blood and Tissue Kit (Qiagen, hereafter “DNeasy”) and PowerSoil DNA Isolation Kit (Mo Bio Laboratories, hereafter “PowerSoil”) following the manufacturers’ protocols. .. DNA yields from tunic extractions were quantified on a NanoDrop One spectrophotometer (Thermo Scientific, Waltham, MA, USA).

    Article Title: Effects of environmental variation and spatial distance on Bacteria, Archaea and viruses in sub-polar and arctic waters
    Article Snippet: .. Upon thawing, nucleic acids were extracted using a PowerSoil DNA Isolation kit (cat. no. 12888-100; Mo Bio, Carlsbad, CA, USA) using the alternative lysis protocol involving two heating steps to 70 °C for 5 min. .. The extracts had a volume of 100 μl in solution S6 (contains no EDTA) and subsamples were used directly in subsequent PCR amplifications.

    Lysis:

    Article Title: Effects of environmental variation and spatial distance on Bacteria, Archaea and viruses in sub-polar and arctic waters
    Article Snippet: .. Upon thawing, nucleic acids were extracted using a PowerSoil DNA Isolation kit (cat. no. 12888-100; Mo Bio, Carlsbad, CA, USA) using the alternative lysis protocol involving two heating steps to 70 °C for 5 min. .. The extracts had a volume of 100 μl in solution S6 (contains no EDTA) and subsamples were used directly in subsequent PCR amplifications.

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  • 99
    Qiagen dneasy powersoil kit
    Dneasy Powersoil Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dneasy powersoil kit/product/Qiagen
    Average 99 stars, based on 63 article reviews
    Price from $9.99 to $1999.99
    dneasy powersoil kit - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

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