dna extraction  (Qiagen)


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    Name:
    Solution C3
    Description:
    For the isolation of microbial genomic DNA from all soil types Kit contents DNeasy PowerSoil Isolation Kit Components Benefits Fast and easy protocol isolates high quality DNA from up to 250 mg samples in just 30 minutes Inhibitor Removal Technology removes PCR inhibitors found in tough sample types Optimized to isolate DNA from difficult even the most difficult environmental samp
    Catalog Number:
    12888-100-3
    Price:
    92
    Category:
    DNeasy PowerSoil Kit
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    Structured Review

    Qiagen dna extraction
    Solution C3
    For the isolation of microbial genomic DNA from all soil types Kit contents DNeasy PowerSoil Isolation Kit Components Benefits Fast and easy protocol isolates high quality DNA from up to 250 mg samples in just 30 minutes Inhibitor Removal Technology removes PCR inhibitors found in tough sample types Optimized to isolate DNA from difficult even the most difficult environmental samp
    https://www.bioz.com/result/dna extraction/product/Qiagen
    Average 99 stars, based on 3012 article reviews
    Price from $9.99 to $1999.99
    dna extraction - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    DNA Extraction:

    Article Title: Inter-personal diversity and temporal dynamics of dental, tongue, and salivary microbiota in the healthy oral cavity
    Article Snippet: Paragraph title: DNA extraction and sequencing ... Samples were divided in two tubes prior to adding Solution C3 (MO BIO) and subsequently mixed onto a single spin filter.

    Amplification:

    Article Title: Inter-personal diversity and temporal dynamics of dental, tongue, and salivary microbiota in the healthy oral cavity
    Article Snippet: Samples were divided in two tubes prior to adding Solution C3 (MO BIO) and subsequently mixed onto a single spin filter. .. The V3–V4 regions of the 16S rRNA gene were amplified using bacterial primer 341F and universal primer 806R.

    Article Title: Antiretroviral Therapy Administration in Healthy Rhesus Macaques Is Associated with Transient Shifts in Intestinal Bacterial Diversity and Modest Immunological Perturbations
    Article Snippet: .. Supernatant was transferred to a 96-well deep-well plate (Costar) and processed as follows with solution addition and supernatant transfer/removal facilitated using a Biomek NKP (Beckman Coulter) system: (step 1) 250 μl of solution C2 (Qiagen) was added to supernatant and mixed at 1,000 rpm for 10 min at room temperature on an orbital shaker; (step 2) the 96-well plate was sealed with an aluminum plate sealer and centrifuged at 4,500 × g for 10 min at 4°C; (step 3) 580 μl of supernatant was transferred to a new 96-well deep-well plate; (step 4) 200 μl of solution C3 (Qiagen) was added to the supernatant and mixed at 1,000 rpm for 10 min at room temperature; (step 5) 0.6 ng of an internal amplification control (GenBank accession number ) was added to each well as an internal amplification control; (step 6) the 96-well plate was sealed with an aluminum plate sealer and centrifuged at 4,500 × g for 10 min at 4°C; (step 7) 450 μl of supernatant was transferred to a new 96-well deep-well plate; (step 8) 450 μl of ClearMag (Qiagen) magnetic bead solution consisting of 19 μl of beads and 431 μl of binding solution was added to the supernatant and mixed at 1,000 rpm for 10 min at room temperature; (step 9) supernatant-containing plate was transferred to rest on a magnetic plate (Magnum FLX; Alpaqua), and bead-bound DNA was allowed to precipitate for 15 min at room temperature; (step 10) 820 μl of supernatant was removed following precipitation; (step 11) the deep-well plate was removed from the magnet, and bead-bound DNA washed three times with 500 μl of solution C5-D (Qiagen) on an orbital shaker, with between-wash magnetic pelleting and supernatant removal as in steps 9 and 10; (step 12) after final washing, pelleted DNA was allowed to dry overnight at room temperature; (step 13) 200 μl of sterile water was added to elute the DNA and mixed at 1,000 rpm for 15 min at room temperature; (step 14) the deep-well plate was transferred back to the magnetic plate where DNA-free beads were allowed to pellet for 10 min; (step 15) 200 μl of DNA-containing supernatant was transferred to a new deep-well plate. .. Eluted DNA was cleaned using a Qiagen DNeasy Blood and Tissue kit per the manufacturer’s protocol, beginning with the addition of buffer AL ( ).

    Isolation:

    Article Title: Antiretroviral Therapy Administration in Healthy Rhesus Macaques Is Associated with Transient Shifts in Intestinal Bacterial Diversity and Modest Immunological Perturbations
    Article Snippet: Paragraph title: 16S isolation and analysis. ... Supernatant was transferred to a 96-well deep-well plate (Costar) and processed as follows with solution addition and supernatant transfer/removal facilitated using a Biomek NKP (Beckman Coulter) system: (step 1) 250 μl of solution C2 (Qiagen) was added to supernatant and mixed at 1,000 rpm for 10 min at room temperature on an orbital shaker; (step 2) the 96-well plate was sealed with an aluminum plate sealer and centrifuged at 4,500 × g for 10 min at 4°C; (step 3) 580 μl of supernatant was transferred to a new 96-well deep-well plate; (step 4) 200 μl of solution C3 (Qiagen) was added to the supernatant and mixed at 1,000 rpm for 10 min at room temperature; (step 5) 0.6 ng of an internal amplification control (GenBank accession number ) was added to each well as an internal amplification control; (step 6) the 96-well plate was sealed with an aluminum plate sealer and centrifuged at 4,500 × g for 10 min at 4°C; (step 7) 450 μl of supernatant was transferred to a new 96-well deep-well plate; (step 8) 450 μl of ClearMag (Qiagen) magnetic bead solution consisting of 19 μl of beads and 431 μl of binding solution was added to the supernatant and mixed at 1,000 rpm for 10 min at room temperature; (step 9) supernatant-containing plate was transferred to rest on a magnetic plate (Magnum FLX; Alpaqua), and bead-bound DNA was allowed to precipitate for 15 min at room temperature; (step 10) 820 μl of supernatant was removed following precipitation; (step 11) the deep-well plate was removed from the magnet, and bead-bound DNA washed three times with 500 μl of solution C5-D (Qiagen) on an orbital shaker, with between-wash magnetic pelleting and supernatant removal as in steps 9 and 10; (step 12) after final washing, pelleted DNA was allowed to dry overnight at room temperature; (step 13) 200 μl of sterile water was added to elute the DNA and mixed at 1,000 rpm for 15 min at room temperature; (step 14) the deep-well plate was transferred back to the magnetic plate where DNA-free beads were allowed to pellet for 10 min; (step 15) 200 μl of DNA-containing supernatant was transferred to a new deep-well plate.

    Spectrophotometry:

    Article Title: Inter-personal diversity and temporal dynamics of dental, tongue, and salivary microbiota in the healthy oral cavity
    Article Snippet: Samples were divided in two tubes prior to adding Solution C3 (MO BIO) and subsequently mixed onto a single spin filter. .. DNA concentrations were determined by UV spectrophotometry (Ultrospec 3000, Pharmacia Biotech) at a wavelength of 260 nm.

    Purification:

    Article Title: Antiretroviral Therapy Administration in Healthy Rhesus Macaques Is Associated with Transient Shifts in Intestinal Bacterial Diversity and Modest Immunological Perturbations
    Article Snippet: Supernatant was transferred to a 96-well deep-well plate (Costar) and processed as follows with solution addition and supernatant transfer/removal facilitated using a Biomek NKP (Beckman Coulter) system: (step 1) 250 μl of solution C2 (Qiagen) was added to supernatant and mixed at 1,000 rpm for 10 min at room temperature on an orbital shaker; (step 2) the 96-well plate was sealed with an aluminum plate sealer and centrifuged at 4,500 × g for 10 min at 4°C; (step 3) 580 μl of supernatant was transferred to a new 96-well deep-well plate; (step 4) 200 μl of solution C3 (Qiagen) was added to the supernatant and mixed at 1,000 rpm for 10 min at room temperature; (step 5) 0.6 ng of an internal amplification control (GenBank accession number ) was added to each well as an internal amplification control; (step 6) the 96-well plate was sealed with an aluminum plate sealer and centrifuged at 4,500 × g for 10 min at 4°C; (step 7) 450 μl of supernatant was transferred to a new 96-well deep-well plate; (step 8) 450 μl of ClearMag (Qiagen) magnetic bead solution consisting of 19 μl of beads and 431 μl of binding solution was added to the supernatant and mixed at 1,000 rpm for 10 min at room temperature; (step 9) supernatant-containing plate was transferred to rest on a magnetic plate (Magnum FLX; Alpaqua), and bead-bound DNA was allowed to precipitate for 15 min at room temperature; (step 10) 820 μl of supernatant was removed following precipitation; (step 11) the deep-well plate was removed from the magnet, and bead-bound DNA washed three times with 500 μl of solution C5-D (Qiagen) on an orbital shaker, with between-wash magnetic pelleting and supernatant removal as in steps 9 and 10; (step 12) after final washing, pelleted DNA was allowed to dry overnight at room temperature; (step 13) 200 μl of sterile water was added to elute the DNA and mixed at 1,000 rpm for 15 min at room temperature; (step 14) the deep-well plate was transferred back to the magnetic plate where DNA-free beads were allowed to pellet for 10 min; (step 15) 200 μl of DNA-containing supernatant was transferred to a new deep-well plate. .. 16S amplicons were purified using Agencourt AMPure XP PCR purification (Beckman Coulter) both before and after index amplification.

    Sequencing:

    Article Title: Inter-personal diversity and temporal dynamics of dental, tongue, and salivary microbiota in the healthy oral cavity
    Article Snippet: Paragraph title: DNA extraction and sequencing ... Samples were divided in two tubes prior to adding Solution C3 (MO BIO) and subsequently mixed onto a single spin filter.

    Article Title: Antiretroviral Therapy Administration in Healthy Rhesus Macaques Is Associated with Transient Shifts in Intestinal Bacterial Diversity and Modest Immunological Perturbations
    Article Snippet: Supernatant was transferred to a 96-well deep-well plate (Costar) and processed as follows with solution addition and supernatant transfer/removal facilitated using a Biomek NKP (Beckman Coulter) system: (step 1) 250 μl of solution C2 (Qiagen) was added to supernatant and mixed at 1,000 rpm for 10 min at room temperature on an orbital shaker; (step 2) the 96-well plate was sealed with an aluminum plate sealer and centrifuged at 4,500 × g for 10 min at 4°C; (step 3) 580 μl of supernatant was transferred to a new 96-well deep-well plate; (step 4) 200 μl of solution C3 (Qiagen) was added to the supernatant and mixed at 1,000 rpm for 10 min at room temperature; (step 5) 0.6 ng of an internal amplification control (GenBank accession number ) was added to each well as an internal amplification control; (step 6) the 96-well plate was sealed with an aluminum plate sealer and centrifuged at 4,500 × g for 10 min at 4°C; (step 7) 450 μl of supernatant was transferred to a new 96-well deep-well plate; (step 8) 450 μl of ClearMag (Qiagen) magnetic bead solution consisting of 19 μl of beads and 431 μl of binding solution was added to the supernatant and mixed at 1,000 rpm for 10 min at room temperature; (step 9) supernatant-containing plate was transferred to rest on a magnetic plate (Magnum FLX; Alpaqua), and bead-bound DNA was allowed to precipitate for 15 min at room temperature; (step 10) 820 μl of supernatant was removed following precipitation; (step 11) the deep-well plate was removed from the magnet, and bead-bound DNA washed three times with 500 μl of solution C5-D (Qiagen) on an orbital shaker, with between-wash magnetic pelleting and supernatant removal as in steps 9 and 10; (step 12) after final washing, pelleted DNA was allowed to dry overnight at room temperature; (step 13) 200 μl of sterile water was added to elute the DNA and mixed at 1,000 rpm for 15 min at room temperature; (step 14) the deep-well plate was transferred back to the magnetic plate where DNA-free beads were allowed to pellet for 10 min; (step 15) 200 μl of DNA-containing supernatant was transferred to a new deep-well plate. .. Total DNA (105 ng) was subjected to dual-index amplification and sequencing for nonhuman primate fecal pellets, using barcoded universal primers spanning base pairs 515(F) to 806(R) of the bacterial 16S rRNA V4 region.

    Polymerase Chain Reaction:

    Article Title: Antiretroviral Therapy Administration in Healthy Rhesus Macaques Is Associated with Transient Shifts in Intestinal Bacterial Diversity and Modest Immunological Perturbations
    Article Snippet: Supernatant was transferred to a 96-well deep-well plate (Costar) and processed as follows with solution addition and supernatant transfer/removal facilitated using a Biomek NKP (Beckman Coulter) system: (step 1) 250 μl of solution C2 (Qiagen) was added to supernatant and mixed at 1,000 rpm for 10 min at room temperature on an orbital shaker; (step 2) the 96-well plate was sealed with an aluminum plate sealer and centrifuged at 4,500 × g for 10 min at 4°C; (step 3) 580 μl of supernatant was transferred to a new 96-well deep-well plate; (step 4) 200 μl of solution C3 (Qiagen) was added to the supernatant and mixed at 1,000 rpm for 10 min at room temperature; (step 5) 0.6 ng of an internal amplification control (GenBank accession number ) was added to each well as an internal amplification control; (step 6) the 96-well plate was sealed with an aluminum plate sealer and centrifuged at 4,500 × g for 10 min at 4°C; (step 7) 450 μl of supernatant was transferred to a new 96-well deep-well plate; (step 8) 450 μl of ClearMag (Qiagen) magnetic bead solution consisting of 19 μl of beads and 431 μl of binding solution was added to the supernatant and mixed at 1,000 rpm for 10 min at room temperature; (step 9) supernatant-containing plate was transferred to rest on a magnetic plate (Magnum FLX; Alpaqua), and bead-bound DNA was allowed to precipitate for 15 min at room temperature; (step 10) 820 μl of supernatant was removed following precipitation; (step 11) the deep-well plate was removed from the magnet, and bead-bound DNA washed three times with 500 μl of solution C5-D (Qiagen) on an orbital shaker, with between-wash magnetic pelleting and supernatant removal as in steps 9 and 10; (step 12) after final washing, pelleted DNA was allowed to dry overnight at room temperature; (step 13) 200 μl of sterile water was added to elute the DNA and mixed at 1,000 rpm for 15 min at room temperature; (step 14) the deep-well plate was transferred back to the magnetic plate where DNA-free beads were allowed to pellet for 10 min; (step 15) 200 μl of DNA-containing supernatant was transferred to a new deep-well plate. .. 16S amplicons were purified using Agencourt AMPure XP PCR purification (Beckman Coulter) both before and after index amplification.

    Binding Assay:

    Article Title: Antiretroviral Therapy Administration in Healthy Rhesus Macaques Is Associated with Transient Shifts in Intestinal Bacterial Diversity and Modest Immunological Perturbations
    Article Snippet: .. Supernatant was transferred to a 96-well deep-well plate (Costar) and processed as follows with solution addition and supernatant transfer/removal facilitated using a Biomek NKP (Beckman Coulter) system: (step 1) 250 μl of solution C2 (Qiagen) was added to supernatant and mixed at 1,000 rpm for 10 min at room temperature on an orbital shaker; (step 2) the 96-well plate was sealed with an aluminum plate sealer and centrifuged at 4,500 × g for 10 min at 4°C; (step 3) 580 μl of supernatant was transferred to a new 96-well deep-well plate; (step 4) 200 μl of solution C3 (Qiagen) was added to the supernatant and mixed at 1,000 rpm for 10 min at room temperature; (step 5) 0.6 ng of an internal amplification control (GenBank accession number ) was added to each well as an internal amplification control; (step 6) the 96-well plate was sealed with an aluminum plate sealer and centrifuged at 4,500 × g for 10 min at 4°C; (step 7) 450 μl of supernatant was transferred to a new 96-well deep-well plate; (step 8) 450 μl of ClearMag (Qiagen) magnetic bead solution consisting of 19 μl of beads and 431 μl of binding solution was added to the supernatant and mixed at 1,000 rpm for 10 min at room temperature; (step 9) supernatant-containing plate was transferred to rest on a magnetic plate (Magnum FLX; Alpaqua), and bead-bound DNA was allowed to precipitate for 15 min at room temperature; (step 10) 820 μl of supernatant was removed following precipitation; (step 11) the deep-well plate was removed from the magnet, and bead-bound DNA washed three times with 500 μl of solution C5-D (Qiagen) on an orbital shaker, with between-wash magnetic pelleting and supernatant removal as in steps 9 and 10; (step 12) after final washing, pelleted DNA was allowed to dry overnight at room temperature; (step 13) 200 μl of sterile water was added to elute the DNA and mixed at 1,000 rpm for 15 min at room temperature; (step 14) the deep-well plate was transferred back to the magnetic plate where DNA-free beads were allowed to pellet for 10 min; (step 15) 200 μl of DNA-containing supernatant was transferred to a new deep-well plate. .. Eluted DNA was cleaned using a Qiagen DNeasy Blood and Tissue kit per the manufacturer’s protocol, beginning with the addition of buffer AL ( ).

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  • 99
    Qiagen solution c3
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