powersoil dna isolation kit  (Qiagen)


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    Name:
    DNeasy PowerSoil Kit
    Description:
    For the isolation of microbial genomic DNA from all soil types Kit contents For the isolation of microbial genomic DNA from all soil types Benefits Fast and easy protocol isolates high quality DNA from up to 250 mg samples in just 30 minutes Inhibitor Removal Technology removes PCR inhibitors found in tough sample types Optimized to isolate DNA from difficult even the most difficult environmental samp
    Catalog Number:
    12888-100
    Price:
    550
    Category:
    DNeasy PowerSoil Kit
    Buy from Supplier


    Structured Review

    Qiagen powersoil dna isolation kit
    DNeasy PowerSoil Kit
    For the isolation of microbial genomic DNA from all soil types Kit contents For the isolation of microbial genomic DNA from all soil types Benefits Fast and easy protocol isolates high quality DNA from up to 250 mg samples in just 30 minutes Inhibitor Removal Technology removes PCR inhibitors found in tough sample types Optimized to isolate DNA from difficult even the most difficult environmental samp
    https://www.bioz.com/result/powersoil dna isolation kit/product/Qiagen
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    powersoil dna isolation kit - by Bioz Stars, 2021-06
    99/100 stars

    Images

    1) Product Images from "Comparing Two Common DNA Extraction Kits for the Characterization of Symbiotic Microbial Communities from Ascidian Tissue"

    Article Title: Comparing Two Common DNA Extraction Kits for the Characterization of Symbiotic Microbial Communities from Ascidian Tissue

    Journal: Microbes and Environments

    doi: 10.1264/jsme2.ME18031

    Non-metric multi-dimensional scaling plot based on the Bray-Curtis similarity of microbial communities obtained using DNeasy (black triangles) and PowerSoil (gray circles) kits. Circles encompass samples clustering at > 30% similarity and correspond to replicates of Clavelina oblonga (dashed line) and Polyandrocarpa anguinea (solid line), indicating a high degree of host-specificity and low technical variation across DNA extraction methods.
    Figure Legend Snippet: Non-metric multi-dimensional scaling plot based on the Bray-Curtis similarity of microbial communities obtained using DNeasy (black triangles) and PowerSoil (gray circles) kits. Circles encompass samples clustering at > 30% similarity and correspond to replicates of Clavelina oblonga (dashed line) and Polyandrocarpa anguinea (solid line), indicating a high degree of host-specificity and low technical variation across DNA extraction methods.

    Techniques Used: DNA Extraction

    2) Product Images from "Comparing Two Common DNA Extraction Kits for the Characterization of Symbiotic Microbial Communities from Ascidian Tissue"

    Article Title: Comparing Two Common DNA Extraction Kits for the Characterization of Symbiotic Microbial Communities from Ascidian Tissue

    Journal: Microbes and Environments

    doi: 10.1264/jsme2.ME18031

    Non-metric multi-dimensional scaling plot based on the Bray-Curtis similarity of microbial communities obtained using DNeasy (black triangles) and PowerSoil (gray circles) kits. Circles encompass samples clustering at > 30% similarity and correspond to replicates of Clavelina oblonga (dashed line) and Polyandrocarpa anguinea (solid line), indicating a high degree of host-specificity and low technical variation across DNA extraction methods.
    Figure Legend Snippet: Non-metric multi-dimensional scaling plot based on the Bray-Curtis similarity of microbial communities obtained using DNeasy (black triangles) and PowerSoil (gray circles) kits. Circles encompass samples clustering at > 30% similarity and correspond to replicates of Clavelina oblonga (dashed line) and Polyandrocarpa anguinea (solid line), indicating a high degree of host-specificity and low technical variation across DNA extraction methods.

    Techniques Used: DNA Extraction

    Related Articles

    DNA Extraction:

    Article Title: Comparing Two Common DNA Extraction Kits for the Characterization of Symbiotic Microbial Communities from Ascidian Tissue
    Article Snippet: .. To compare the efficacy of the two DNA extraction kits, tunic tissue from each replicate was processed separately using the DNeasy Blood and Tissue Kit (Qiagen, hereafter “DNeasy”) and PowerSoil DNA Isolation Kit (Mo Bio Laboratories, hereafter “PowerSoil”) following the manufacturers’ protocols. .. DNA yields from tunic extractions were quantified on a NanoDrop One spectrophotometer (Thermo Scientific, Waltham, MA, USA).

    Article Title: Predicting Clinical Outcomes of Cirrhosis Patients With Hepatic Encephalopathy From the Fecal Microbiome
    Article Snippet: We collected 20 stool samples from patients with compensated cirrhosis (without a history of ascites, HE, variceal hemorrhage, and associated severe complication) (C2), 15 samples from patient with advanced stages (CTP score > 7) of cirrhosis with no overt HE 6 months before sampling (C3) and 13 from healthy individuals (C1) as control subjects ( ). .. DNA Extraction and Amplicon Library Construction and Sequencing Total genomic DNA extraction protocol from stool samples was a modification of the PowerSoil DNA isolation kit (Cat. #12888-100; MoBio, Carlsbad, CA), taken from the Human Microbiome Project ( http://hmpdacc.org/doc/HMP_MOP_Version12_0_072910.pdf ). .. In the initial processing of the stool samples, 1.0 g of sample was resuspended in 2.0 mL of MoBio bead solution by vigorous vortexing for 30 seconds in a 15-mL Falcon tube.

    Article Title: Comparing Two Common DNA Extraction Kits for the Characterization of Symbiotic Microbial Communities from Ascidian Tissue
    Article Snippet: Various DNA extraction methods are often used interchangeably for the characterization of microbial communities despite indications that different techniques produce disparate results. .. The microbiomes of two ascidian species were herein characterized using two common DNA extraction kits, the DNeasy Blood and Tissue Kit (Qiagen) and the PowerSoil DNA Isolation Kit (Mo Bio Laboratories), followed by next-generation (Illumina) sequencing of partial 16S rRNA genes. ..

    Centrifugation:

    Article Title: The Microbiome of the Cosmopolitan Diatom Leptocylindrus Reveals Significant Spatial and Temporal Variability
    Article Snippet: .. On day 18 (∼stationary phase) each replicate strain was harvested by centrifugation (2300 RCF for 5 min), and total DNA extracted from the pellet using a DNeasy® PowerSoil® Kit (Qiagen) as per manufacturer’s protocol. .. Bulk water samples were filtered through 0.22 μm pore-size membrane filters (Millipore) using a peristaltic pump and stored at -80°C prior to DNA extraction.

    Amplification:

    Article Title: Predicting Clinical Outcomes of Cirrhosis Patients With Hepatic Encephalopathy From the Fecal Microbiome
    Article Snippet: We collected 20 stool samples from patients with compensated cirrhosis (without a history of ascites, HE, variceal hemorrhage, and associated severe complication) (C2), 15 samples from patient with advanced stages (CTP score > 7) of cirrhosis with no overt HE 6 months before sampling (C3) and 13 from healthy individuals (C1) as control subjects ( ). .. DNA Extraction and Amplicon Library Construction and Sequencing Total genomic DNA extraction protocol from stool samples was a modification of the PowerSoil DNA isolation kit (Cat. #12888-100; MoBio, Carlsbad, CA), taken from the Human Microbiome Project ( http://hmpdacc.org/doc/HMP_MOP_Version12_0_072910.pdf ). .. In the initial processing of the stool samples, 1.0 g of sample was resuspended in 2.0 mL of MoBio bead solution by vigorous vortexing for 30 seconds in a 15-mL Falcon tube.

    Article Title: Using PCR-Based Sequencing to Diagnose Haycocknema perplexum Infection in Human Myositis Case, Australia
    Article Snippet: On the basis of this clinical evidence and the patient’s history, we suspected an infection with H. perplexum nematodes. .. From a 2-mm3 portion of the same muscle biopsy sample used for the previous histopathologic examination and frozen, we extracted genomic DNA using the DNeasy PowerSoil Kit (QIAGEN, Venlo, the Netherlands) and then subjected 2-μL aliquots of this DNA sample to 2 new nested PCR assays adapted from an established method ( – ) that had not amplified products from the same sample. .. The 2 optimized nested PCRs ( ) successfully amplified products of the expected sizes (≈400 bp for cox-1 and ≈830 bp for SSU ) from this sample, and these amplicons were directly sequenced ( ).

    Sequencing:

    Article Title: Predicting Clinical Outcomes of Cirrhosis Patients With Hepatic Encephalopathy From the Fecal Microbiome
    Article Snippet: We collected 20 stool samples from patients with compensated cirrhosis (without a history of ascites, HE, variceal hemorrhage, and associated severe complication) (C2), 15 samples from patient with advanced stages (CTP score > 7) of cirrhosis with no overt HE 6 months before sampling (C3) and 13 from healthy individuals (C1) as control subjects ( ). .. DNA Extraction and Amplicon Library Construction and Sequencing Total genomic DNA extraction protocol from stool samples was a modification of the PowerSoil DNA isolation kit (Cat. #12888-100; MoBio, Carlsbad, CA), taken from the Human Microbiome Project ( http://hmpdacc.org/doc/HMP_MOP_Version12_0_072910.pdf ). .. In the initial processing of the stool samples, 1.0 g of sample was resuspended in 2.0 mL of MoBio bead solution by vigorous vortexing for 30 seconds in a 15-mL Falcon tube.

    Article Title: Comparing Two Common DNA Extraction Kits for the Characterization of Symbiotic Microbial Communities from Ascidian Tissue
    Article Snippet: Various DNA extraction methods are often used interchangeably for the characterization of microbial communities despite indications that different techniques produce disparate results. .. The microbiomes of two ascidian species were herein characterized using two common DNA extraction kits, the DNeasy Blood and Tissue Kit (Qiagen) and the PowerSoil DNA Isolation Kit (Mo Bio Laboratories), followed by next-generation (Illumina) sequencing of partial 16S rRNA genes. ..

    Modification:

    Article Title: Predicting Clinical Outcomes of Cirrhosis Patients With Hepatic Encephalopathy From the Fecal Microbiome
    Article Snippet: We collected 20 stool samples from patients with compensated cirrhosis (without a history of ascites, HE, variceal hemorrhage, and associated severe complication) (C2), 15 samples from patient with advanced stages (CTP score > 7) of cirrhosis with no overt HE 6 months before sampling (C3) and 13 from healthy individuals (C1) as control subjects ( ). .. DNA Extraction and Amplicon Library Construction and Sequencing Total genomic DNA extraction protocol from stool samples was a modification of the PowerSoil DNA isolation kit (Cat. #12888-100; MoBio, Carlsbad, CA), taken from the Human Microbiome Project ( http://hmpdacc.org/doc/HMP_MOP_Version12_0_072910.pdf ). .. In the initial processing of the stool samples, 1.0 g of sample was resuspended in 2.0 mL of MoBio bead solution by vigorous vortexing for 30 seconds in a 15-mL Falcon tube.

    Nested PCR:

    Article Title: Using PCR-Based Sequencing to Diagnose Haycocknema perplexum Infection in Human Myositis Case, Australia
    Article Snippet: On the basis of this clinical evidence and the patient’s history, we suspected an infection with H. perplexum nematodes. .. From a 2-mm3 portion of the same muscle biopsy sample used for the previous histopathologic examination and frozen, we extracted genomic DNA using the DNeasy PowerSoil Kit (QIAGEN, Venlo, the Netherlands) and then subjected 2-μL aliquots of this DNA sample to 2 new nested PCR assays adapted from an established method ( – ) that had not amplified products from the same sample. .. The 2 optimized nested PCRs ( ) successfully amplified products of the expected sizes (≈400 bp for cox-1 and ≈830 bp for SSU ) from this sample, and these amplicons were directly sequenced ( ).

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  • 86
    Qiagen powersoil dna isolation kit
    Representative results comparing MoBio <t>PowerSoil®,</t> MP Biomedicals™ FastDNA™ SPIN and SDE methods run on a LabChip GX Touch: (a) Representative 16S libraries of gDNA extracted with MoBio PowerSoil®. (b) Representative ITS libraries of gDNA extracted with MoBio PowerSoil®. (c) Representative 16S libraries of gDNA extracted with SDE. (d) Representative ITS libraries of gDNA extracted with SDE. (e) Representative 16S libraries of extracted gDNA with MP Biomedicals™ FastDNA™ SPIN. (f) Representative ITS libraries of extracted gDNA with MP Biomedicals™ FastDNA™ SPIN. The target size of representative 16S libraries is between 350 and 500 bp and for ITS libraries between 300 and 700 bp. gDNA, genomic <t>DNA;</t> SDE, soil gDNA extraction method [Correction added on 11 Dec 2020, after first online publication: Figure 2 has been updated to correct minor inaccuracies.]
    Powersoil Dna Isolation Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/powersoil dna isolation kit/product/Qiagen
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    powersoil dna isolation kit - by Bioz Stars, 2021-06
    86/100 stars
      Buy from Supplier

    Image Search Results


    Representative results comparing MoBio PowerSoil®, MP Biomedicals™ FastDNA™ SPIN and SDE methods run on a LabChip GX Touch: (a) Representative 16S libraries of gDNA extracted with MoBio PowerSoil®. (b) Representative ITS libraries of gDNA extracted with MoBio PowerSoil®. (c) Representative 16S libraries of gDNA extracted with SDE. (d) Representative ITS libraries of gDNA extracted with SDE. (e) Representative 16S libraries of extracted gDNA with MP Biomedicals™ FastDNA™ SPIN. (f) Representative ITS libraries of extracted gDNA with MP Biomedicals™ FastDNA™ SPIN. The target size of representative 16S libraries is between 350 and 500 bp and for ITS libraries between 300 and 700 bp. gDNA, genomic DNA; SDE, soil gDNA extraction method [Correction added on 11 Dec 2020, after first online publication: Figure 2 has been updated to correct minor inaccuracies.]

    Journal: MicrobiologyOpen

    Article Title: A low‐cost pipeline for soil microbiome profiling

    doi: 10.1002/mbo3.1133

    Figure Lengend Snippet: Representative results comparing MoBio PowerSoil®, MP Biomedicals™ FastDNA™ SPIN and SDE methods run on a LabChip GX Touch: (a) Representative 16S libraries of gDNA extracted with MoBio PowerSoil®. (b) Representative ITS libraries of gDNA extracted with MoBio PowerSoil®. (c) Representative 16S libraries of gDNA extracted with SDE. (d) Representative ITS libraries of gDNA extracted with SDE. (e) Representative 16S libraries of extracted gDNA with MP Biomedicals™ FastDNA™ SPIN. (f) Representative ITS libraries of extracted gDNA with MP Biomedicals™ FastDNA™ SPIN. The target size of representative 16S libraries is between 350 and 500 bp and for ITS libraries between 300 and 700 bp. gDNA, genomic DNA; SDE, soil gDNA extraction method [Correction added on 11 Dec 2020, after first online publication: Figure 2 has been updated to correct minor inaccuracies.]

    Article Snippet: We tested four different extraction methods: (a) the PowerSoil® DNA Isolation Kit (12888‐50; CAMBIO, now 12888‐100, DNeasy PowerSoil, QIAGEN Ltd); (b) the MP Biomedicals™ FastDNA™ SPIN Kit for Soil (11492400; Fisher Scientific); (c) our SDE method; and (d) the recently published paperdisk method (Zou et al., ).

    Techniques:

    Genomic TapeStation traces of representative MoBio PowerSoil®, MP Biomedicals™ FastDNA™ SPIN and SDE gDNA extractions. gDNA, genomic DNA; SDE, soil gDNA extraction method

    Journal: MicrobiologyOpen

    Article Title: A low‐cost pipeline for soil microbiome profiling

    doi: 10.1002/mbo3.1133

    Figure Lengend Snippet: Genomic TapeStation traces of representative MoBio PowerSoil®, MP Biomedicals™ FastDNA™ SPIN and SDE gDNA extractions. gDNA, genomic DNA; SDE, soil gDNA extraction method

    Article Snippet: We tested four different extraction methods: (a) the PowerSoil® DNA Isolation Kit (12888‐50; CAMBIO, now 12888‐100, DNeasy PowerSoil, QIAGEN Ltd); (b) the MP Biomedicals™ FastDNA™ SPIN Kit for Soil (11492400; Fisher Scientific); (c) our SDE method; and (d) the recently published paperdisk method (Zou et al., ).

    Techniques:

    PCoA comparison of ASV abundances and relative abundance bar chart for bacterial and fungal communities of three different gDNA extraction methods and four soil types: (a) Top 30 bacterial community composition representing 0.72% of the overall bacterial community. (b) PCoA showing beta diversity by Bray–Curtis distance for bacterial community composition. (c) Top 30 fungal community composition representing 1.87% of the overall fungal community. (d) PCoA showing beta diversity by Bray–Curtis distance for fungal community composition. (a) and (b) show that the clustering for the entire bacterial communities is soil type‐dependent and not driven by different gDNA extraction methods. (c) and (d) show the same is true for the fungal community structure. Statistical analysis shows no significant difference between DNA extraction methods used for both bacterial (adonis test, p ‐value: .579) and fungal (adonis test, p ‐value: .801) communities, but shows a significant difference between locations for bacterial (ANOSIM test, p ‐value: 9.99e–4) and fungal (ANOSIM test, p ‐value: 9.99e‐4) communities. ASVs, amplicon sequence variants; BrF, broad‐leafed forest soil; Cer, standard cereal compost used at the JIC; CoF, coniferous forest soil; MiF, mixed forest soil; SDE, soil gDNA extraction method, MoBio, MoBio PowerSoil®; MP, MP Biomedicals™ FastDNA™ SPIN

    Journal: MicrobiologyOpen

    Article Title: A low‐cost pipeline for soil microbiome profiling

    doi: 10.1002/mbo3.1133

    Figure Lengend Snippet: PCoA comparison of ASV abundances and relative abundance bar chart for bacterial and fungal communities of three different gDNA extraction methods and four soil types: (a) Top 30 bacterial community composition representing 0.72% of the overall bacterial community. (b) PCoA showing beta diversity by Bray–Curtis distance for bacterial community composition. (c) Top 30 fungal community composition representing 1.87% of the overall fungal community. (d) PCoA showing beta diversity by Bray–Curtis distance for fungal community composition. (a) and (b) show that the clustering for the entire bacterial communities is soil type‐dependent and not driven by different gDNA extraction methods. (c) and (d) show the same is true for the fungal community structure. Statistical analysis shows no significant difference between DNA extraction methods used for both bacterial (adonis test, p ‐value: .579) and fungal (adonis test, p ‐value: .801) communities, but shows a significant difference between locations for bacterial (ANOSIM test, p ‐value: 9.99e–4) and fungal (ANOSIM test, p ‐value: 9.99e‐4) communities. ASVs, amplicon sequence variants; BrF, broad‐leafed forest soil; Cer, standard cereal compost used at the JIC; CoF, coniferous forest soil; MiF, mixed forest soil; SDE, soil gDNA extraction method, MoBio, MoBio PowerSoil®; MP, MP Biomedicals™ FastDNA™ SPIN

    Article Snippet: We tested four different extraction methods: (a) the PowerSoil® DNA Isolation Kit (12888‐50; CAMBIO, now 12888‐100, DNeasy PowerSoil, QIAGEN Ltd); (b) the MP Biomedicals™ FastDNA™ SPIN Kit for Soil (11492400; Fisher Scientific); (c) our SDE method; and (d) the recently published paperdisk method (Zou et al., ).

    Techniques: DNA Extraction, Amplification, Sequencing

    Alpha‐diversity comparison for bacterial and fungal communities of three different gDNA extraction methods and four soil types: (a) Bacterial alpha‐diversity overview of the observed richness and Shannon diversity. No influence on alpha diversity by extraction method (Shannon diversity ANOVA test, p ‐value: .466618, observed richness Kruskal test, p ‐value: .36554), but by soil type (Shannon diversity ANOVA test, p ‐value: .018507, Observed richness Kruskal test, p ‐value: .032519). No influence by soil type on fungal alpha diversity (Shannon diversity Kruskal test, p ‐value: .21473, observed diversity Kruskal test, p ‐value: .103862) and some influence of extraction method (Shannon diversity Kruskal test, p ‐value: .018628, observed richness Kruskal test, p ‐value: .168873). BrF, broad‐leafed forest soil; Cer, standard cereal compost used at the JIC; CoF, coniferous forest soil; gDNA, genomic DNA; MiF, mixed forest soil; MoBio, MoBio PowerSoil®; MP, MP Biomedicals™ FastDNA™ SPIN; SDE, soil gDNA extraction method

    Journal: MicrobiologyOpen

    Article Title: A low‐cost pipeline for soil microbiome profiling

    doi: 10.1002/mbo3.1133

    Figure Lengend Snippet: Alpha‐diversity comparison for bacterial and fungal communities of three different gDNA extraction methods and four soil types: (a) Bacterial alpha‐diversity overview of the observed richness and Shannon diversity. No influence on alpha diversity by extraction method (Shannon diversity ANOVA test, p ‐value: .466618, observed richness Kruskal test, p ‐value: .36554), but by soil type (Shannon diversity ANOVA test, p ‐value: .018507, Observed richness Kruskal test, p ‐value: .032519). No influence by soil type on fungal alpha diversity (Shannon diversity Kruskal test, p ‐value: .21473, observed diversity Kruskal test, p ‐value: .103862) and some influence of extraction method (Shannon diversity Kruskal test, p ‐value: .018628, observed richness Kruskal test, p ‐value: .168873). BrF, broad‐leafed forest soil; Cer, standard cereal compost used at the JIC; CoF, coniferous forest soil; gDNA, genomic DNA; MiF, mixed forest soil; MoBio, MoBio PowerSoil®; MP, MP Biomedicals™ FastDNA™ SPIN; SDE, soil gDNA extraction method

    Article Snippet: We tested four different extraction methods: (a) the PowerSoil® DNA Isolation Kit (12888‐50; CAMBIO, now 12888‐100, DNeasy PowerSoil, QIAGEN Ltd); (b) the MP Biomedicals™ FastDNA™ SPIN Kit for Soil (11492400; Fisher Scientific); (c) our SDE method; and (d) the recently published paperdisk method (Zou et al., ).

    Techniques:

    Non-metric multi-dimensional scaling plot based on the Bray-Curtis similarity of microbial communities obtained using DNeasy (black triangles) and PowerSoil (gray circles) kits. Circles encompass samples clustering at > 30% similarity and correspond to replicates of Clavelina oblonga (dashed line) and Polyandrocarpa anguinea (solid line), indicating a high degree of host-specificity and low technical variation across DNA extraction methods.

    Journal: Microbes and Environments

    Article Title: Comparing Two Common DNA Extraction Kits for the Characterization of Symbiotic Microbial Communities from Ascidian Tissue

    doi: 10.1264/jsme2.ME18031

    Figure Lengend Snippet: Non-metric multi-dimensional scaling plot based on the Bray-Curtis similarity of microbial communities obtained using DNeasy (black triangles) and PowerSoil (gray circles) kits. Circles encompass samples clustering at > 30% similarity and correspond to replicates of Clavelina oblonga (dashed line) and Polyandrocarpa anguinea (solid line), indicating a high degree of host-specificity and low technical variation across DNA extraction methods.

    Article Snippet: To compare the efficacy of the two DNA extraction kits, tunic tissue from each replicate was processed separately using the DNeasy Blood and Tissue Kit (Qiagen, hereafter “DNeasy”) and PowerSoil DNA Isolation Kit (Mo Bio Laboratories, hereafter “PowerSoil”) following the manufacturers’ protocols.

    Techniques: DNA Extraction