dneasy powersoil kit  (Qiagen)


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    Name:
    DNeasy PowerSoil Kit
    Description:
    For the isolation of microbial genomic DNA from all soil types Kit contents For the isolation of microbial genomic DNA from all soil types Benefits Fast and easy protocol isolates high quality DNA from up to 250 mg samples in just 30 minutes Inhibitor Removal Technology removes PCR inhibitors found in tough sample types Optimized to isolate DNA from difficult even the most difficult environmental samp
    Catalog Number:
    12888-100
    Price:
    550
    Category:
    DNeasy PowerSoil Kit
    Buy from Supplier


    Structured Review

    Qiagen dneasy powersoil kit
    DNeasy PowerSoil Kit
    For the isolation of microbial genomic DNA from all soil types Kit contents For the isolation of microbial genomic DNA from all soil types Benefits Fast and easy protocol isolates high quality DNA from up to 250 mg samples in just 30 minutes Inhibitor Removal Technology removes PCR inhibitors found in tough sample types Optimized to isolate DNA from difficult even the most difficult environmental samp
    https://www.bioz.com/result/dneasy powersoil kit/product/Qiagen
    Average 90 stars, based on 42 article reviews
    Price from $9.99 to $1999.99
    dneasy powersoil kit - by Bioz Stars, 2020-01
    90/100 stars

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    Related Articles

    Clone Assay:

    Article Title: The Microbial Community of a Passive Biochemical Reactor Treating Arsenic, Zinc, and Sulfate-Rich Seepage
    Article Snippet: Cloned inserts were sequenced bi-directionally with M13F (5′-GTAAAACGACGGCCAG) and M13R (5′-CAGGAAACAGCTATGAC) primers at the Michael Smith Genome Sciences Centre (Vancouver, BC, Canada). .. The DNA was extracted using the Power Soil DNA isolation Kit (MoBio Laboratories Incorporation, Carlsbad, CA, USA, Cat No:12888-100) following the manufacturer’s instructions.

    Centrifugation:

    Article Title: High-throughput sequencing data and antibiotic resistance mechanisms of soil microbial communities in non-irrigated and irrigated soils with raw sewage in African cities
    Article Snippet: Low speed centrifugation in 50-mL conical tubes at 160 g for 6 min was used to remove coarse particles, big eukaryotic cells and bacterial flocks. .. Soil DNA was extracted using the DNeasy PowerSoil Kit (Qiagen, Germany) according to the manufacturer's instructions.

    Article Title: Systematic Bias Introduced by Genomic DNA Template Dilution in 16S rRNA Gene-Targeted Microbiota Profiling in Human Stool Homogenates
    Article Snippet: The contents of one PowerBead tube (Mo Bio Laboratories, Inc.; catalog no. 12888-50-PBT) and 60 μl of solution C1 (Mo Bio Laboratories, Inc.; catalog no. 12888-50-1) were added to each aliquot. .. Stool homogenates were clarified by centrifugation, pooled (2-ml total volume), and diluted to 3.4 ml with beading solution (Mo Bio Laboratories, Inc.; catalog no. 12888-50-BS).

    Amplification:

    Article Title: Alfalfa-containing diets alter luminal microbiota structure and short chain fatty acid sensing in the caecal mucosa of pigs
    Article Snippet: .. Bacterial DNA extraction, PCR amplification, and Illumina MiSeq sequencing The total genomic DNA was extracted from caecal digesta using Power Soil DNA Isolation Kit (12888–50; Mobio Technologies Inc., Vancouver, BC) following the manufacture recommendations. ..

    Article Title: Oral microbiome in HIV-associated periodontitis
    Article Snippet: .. 2.3 DNA extraction, 16 s rRNA gene amplification, and sequencing Genomic DNA from all samples was extracted utilizing the MoBio PowerSoil DNA Isolation Kit (Cat # 12888–50) and quantified via Quant-iT PicoGreen dsDNA Assay (Cat # P11496). .. The 16 s V4 region was amplified through 30 cycles PCR with an F515 locus-specific primer (5′-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG-3′) and an 806R-locus specific primer (5′-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG-3′) both with compatible overhang regions for index attachment.

    Article Title: Core Sulphate-Reducing Microorganisms in Metal-Removing Semi-Passive Biochemical Reactors and the Co-Occurrence of Methanogens
    Article Snippet: Paragraph title: 2.2. DNA Extraction, Amplification of SSU rRNA Genes and Pyrosequencing ... After homogenization of the sample under liquid N2 with a pestle and mortar, DNA was extracted from 0.5 g wet weight using a PowerSoil DNA isolation Kit (MoBio Laboratories Incorporation, Carlsbad, CA, USA, Cat No:12888-100).

    Article Title: Childhood Adversity Impact on Gut Microbiota and Inflammatory Response to Stress During Pregnancy
    Article Snippet: DNA was isolated from approximately 200 mg stool using the MoBio PowerSoil® DNA Isolation Kit (Catalog # 12888–50). .. 16S sequencing was performed; primers annealing to the V1–V2 region of the 16S bacterial gene were used for amplification as described previously ( ).

    Article Title: Core Sulphate-Reducing Microorganisms in Metal-Removing Semi-Passive Biochemical Reactors and the Co-Occurrence of Methanogens
    Article Snippet: .. DNA Extraction, Amplification of SSU rRNA Genes and Pyrosequencing After homogenization of the sample under liquid N2 with a pestle and mortar, DNA was extracted from 0.5 g wet weight using a PowerSoil DNA isolation Kit (MoBio Laboratories Incorporation, Carlsbad, CA, USA, Cat No:12888-100). .. Isolated community DNA (2 ng μL−1 ) was subjected to polymerase chain reaction (PCR) amplification of variable region V6 to V8 of SSU rRNA of bacteria and archaea by using barcoded primer pair 926f (AAA CTY AAA KGA ATT GAC GG), 1392r (ACG GGC GGT GTG TRC) in an iCycler® (Bio-Rad Laboratories Ltd., Meyerside Drive, ON, Canada) thermocycler under conditions: 95 °C for 3 min; 25 cycles of 95 °C for 30 s, 55 °C for 45 s, 72 °C for 90 s; and 72 °C for 10 min. DNA was purified using the QIAquick® PCR purification kit (Qiagen, Toronto, ON, Canada).

    Article Title: The Microbial Community of a Passive Biochemical Reactor Treating Arsenic, Zinc, and Sulfate-Rich Seepage
    Article Snippet: The DNA was extracted using the Power Soil DNA isolation Kit (MoBio Laboratories Incorporation, Carlsbad, CA, USA, Cat No:12888-100) following the manufacturer’s instructions. .. DNA from adjacent 5-cm intervals was combined to yield 38 samples from all cores and all depths that were used for PCR amplification of the V6–V8 variable SSU rRNA region: 926f (5′ AAACTYAAAKGAATTGRCGG 3′) and 1392r (5′ ACGGGCGGTGTGTRC 3′) as described in detail in the Supplemental Material.

    Article Title: Early-Onset Preeclampsia Is Associated With Gut Microbial Alterations in Antepartum and Postpartum Women
    Article Snippet: DNA Extraction and 16S rRNA Gene Sequencing The microbial genomic DNA was extracted according to the MOBIO PowerSoil® DNA Isolation Kit 12888-100 protocol, and DNA was stored at −80°C in Tris-EDTA buffer solution before use. .. To enable amplification of the V4 region of the 16S rRNA gene and add barcode sequences, unique fusion primers were designed based on the universal primers set, 515F (5′-GTGYCAGCMGCCGCGGTAA-3′) and 806R (5′-GGACTACNVGGGTWTCTAAT-3′), along with barcode sequences.

    Whole Genome Amplification:

    Article Title: Axiom Microbiome Array, the next generation microarray for high-throughput pathogen and microbiome analysis
    Article Snippet: Soil was collected from locations in San Francisco as described previously [ ] and DNA was extracted using the MoBio PowerSoil extraction kit (Catalog number: 12888–50). .. Soil DNA was quantified using the Qubit spectrophotometer and each quantity of microbial DNA was spiked into 1.0 ng of soil DNA prior to whole genome amplification in the Axiom microarray process.

    Picogreen Assay:

    Article Title: High-throughput sequencing data and antibiotic resistance mechanisms of soil microbial communities in non-irrigated and irrigated soils with raw sewage in African cities
    Article Snippet: Soil DNA was extracted using the DNeasy PowerSoil Kit (Qiagen, Germany) according to the manufacturer's instructions. .. DNA concentration was determined by using the Quant-iT PicoGreen dsDNA Assay Kit, and the Qubit™ 3.0 Fluorometer (Qubit, Life Technologies, USA).

    Article Title: Oral microbiome in HIV-associated periodontitis
    Article Snippet: .. 2.3 DNA extraction, 16 s rRNA gene amplification, and sequencing Genomic DNA from all samples was extracted utilizing the MoBio PowerSoil DNA Isolation Kit (Cat # 12888–50) and quantified via Quant-iT PicoGreen dsDNA Assay (Cat # P11496). .. The 16 s V4 region was amplified through 30 cycles PCR with an F515 locus-specific primer (5′-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG-3′) and an 806R-locus specific primer (5′-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG-3′) both with compatible overhang regions for index attachment.

    Real-time Polymerase Chain Reaction:

    Article Title: Axiom Microbiome Array, the next generation microarray for high-throughput pathogen and microbiome analysis
    Article Snippet: DNA sample acquisition and quantitation Vaccinia Lister DNA was obtained from Advanced Biotechnologies Inc. (Columbia, MD) and copy number quantitation was performed by Advanced Biotechnologies using real-time PCR on the Roche LightCycler. .. Soil was collected from locations in San Francisco as described previously [ ] and DNA was extracted using the MoBio PowerSoil extraction kit (Catalog number: 12888–50).

    Microarray:

    Article Title: Axiom Microbiome Array, the next generation microarray for high-throughput pathogen and microbiome analysis
    Article Snippet: Soil was collected from locations in San Francisco as described previously [ ] and DNA was extracted using the MoBio PowerSoil extraction kit (Catalog number: 12888–50). .. Soil DNA was quantified using the Qubit spectrophotometer and each quantity of microbial DNA was spiked into 1.0 ng of soil DNA prior to whole genome amplification in the Axiom microarray process.

    Quantitation Assay:

    Article Title: Axiom Microbiome Array, the next generation microarray for high-throughput pathogen and microbiome analysis
    Article Snippet: Paragraph title: DNA sample acquisition and quantitation ... Soil was collected from locations in San Francisco as described previously [ ] and DNA was extracted using the MoBio PowerSoil extraction kit (Catalog number: 12888–50).

    Infection:

    Article Title: STAT2 dependent Type I Interferon response promotes dysbiosis and luminal expansion of the enteric pathogen Salmonella Typhimurium
    Article Snippet: .. Microbiota analysis DNA from fecal contents of wild-type and Stat2 -/- infected mice was extracted using the PowerSoil DNA Isolation Kit (MoBio, 12888–50) according to manufacturer’s protocol. .. High quality isolated DNA was then submitted to SeqMatic for 16S rRNA V4 sequencing using the Illumina MiSeq platform.

    Sequencing:

    Article Title: Metagenomic Analysis of Microbial Community Compositions and Cold-Responsive Stress Genes in Selected Antarctic Lacustrine and Soil Ecosystems
    Article Snippet: Paragraph title: 2.2. DNA Extraction and Sequencing ... The LU_sediment samples (1 g each) and the membrane filters were subjected to DNA extraction by using the MoBio PowerSoil® DNA Isolation Kit (MoBio Laboratories Inc., Carlsbad, CA, USA; cat # 12888–100).

    Article Title: Alfalfa-containing diets alter luminal microbiota structure and short chain fatty acid sensing in the caecal mucosa of pigs
    Article Snippet: .. Bacterial DNA extraction, PCR amplification, and Illumina MiSeq sequencing The total genomic DNA was extracted from caecal digesta using Power Soil DNA Isolation Kit (12888–50; Mobio Technologies Inc., Vancouver, BC) following the manufacture recommendations. ..

    Article Title: High-throughput sequencing data and antibiotic resistance mechanisms of soil microbial communities in non-irrigated and irrigated soils with raw sewage in African cities
    Article Snippet: Soil DNA was extracted using the DNeasy PowerSoil Kit (Qiagen, Germany) according to the manufacturer's instructions. .. Six DNA samples representative of the three cities were sent to Edinburgh Genomics for high-throughput sequencing.

    Article Title: Oral microbiome in HIV-associated periodontitis
    Article Snippet: .. 2.3 DNA extraction, 16 s rRNA gene amplification, and sequencing Genomic DNA from all samples was extracted utilizing the MoBio PowerSoil DNA Isolation Kit (Cat # 12888–50) and quantified via Quant-iT PicoGreen dsDNA Assay (Cat # P11496). .. The 16 s V4 region was amplified through 30 cycles PCR with an F515 locus-specific primer (5′-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG-3′) and an 806R-locus specific primer (5′-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG-3′) both with compatible overhang regions for index attachment.

    Article Title: Childhood Adversity Impact on Gut Microbiota and Inflammatory Response to Stress During Pregnancy
    Article Snippet: Paragraph title: 2.3. Stool sampling and sequencing. ... DNA was isolated from approximately 200 mg stool using the MoBio PowerSoil® DNA Isolation Kit (Catalog # 12888–50).

    Article Title: STAT2 dependent Type I Interferon response promotes dysbiosis and luminal expansion of the enteric pathogen Salmonella Typhimurium
    Article Snippet: Microbiota analysis DNA from fecal contents of wild-type and Stat2 -/- infected mice was extracted using the PowerSoil DNA Isolation Kit (MoBio, 12888–50) according to manufacturer’s protocol. .. High quality isolated DNA was then submitted to SeqMatic for 16S rRNA V4 sequencing using the Illumina MiSeq platform.

    Article Title: Early-Onset Preeclampsia Is Associated With Gut Microbial Alterations in Antepartum and Postpartum Women
    Article Snippet: .. DNA Extraction and 16S rRNA Gene Sequencing The microbial genomic DNA was extracted according to the MOBIO PowerSoil® DNA Isolation Kit 12888-100 protocol, and DNA was stored at −80°C in Tris-EDTA buffer solution before use. .. To enable amplification of the V4 region of the 16S rRNA gene and add barcode sequences, unique fusion primers were designed based on the universal primers set, 515F (5′-GTGYCAGCMGCCGCGGTAA-3′) and 806R (5′-GGACTACNVGGGTWTCTAAT-3′), along with barcode sequences.

    DNA Extraction:

    Article Title: Metagenomic Analysis of Microbial Community Compositions and Cold-Responsive Stress Genes in Selected Antarctic Lacustrine and Soil Ecosystems
    Article Snippet: .. The LU_sediment samples (1 g each) and the membrane filters were subjected to DNA extraction by using the MoBio PowerSoil® DNA Isolation Kit (MoBio Laboratories Inc., Carlsbad, CA, USA; cat # 12888–100). .. Briefly, each sample was transferred into separate 2 mL PowerBead tubes and then used for community DNA extraction.

    Article Title: Alfalfa-containing diets alter luminal microbiota structure and short chain fatty acid sensing in the caecal mucosa of pigs
    Article Snippet: .. Bacterial DNA extraction, PCR amplification, and Illumina MiSeq sequencing The total genomic DNA was extracted from caecal digesta using Power Soil DNA Isolation Kit (12888–50; Mobio Technologies Inc., Vancouver, BC) following the manufacture recommendations. ..

    Article Title: Oral microbiome in HIV-associated periodontitis
    Article Snippet: .. 2.3 DNA extraction, 16 s rRNA gene amplification, and sequencing Genomic DNA from all samples was extracted utilizing the MoBio PowerSoil DNA Isolation Kit (Cat # 12888–50) and quantified via Quant-iT PicoGreen dsDNA Assay (Cat # P11496). .. The 16 s V4 region was amplified through 30 cycles PCR with an F515 locus-specific primer (5′-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG-3′) and an 806R-locus specific primer (5′-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG-3′) both with compatible overhang regions for index attachment.

    Article Title: Core Sulphate-Reducing Microorganisms in Metal-Removing Semi-Passive Biochemical Reactors and the Co-Occurrence of Methanogens
    Article Snippet: .. After homogenization of the sample under liquid N2 with a pestle and mortar, DNA was extracted from 0.5 g wet weight using a PowerSoil DNA isolation Kit (MoBio Laboratories Incorporation, Carlsbad, CA, USA, Cat No:12888-100). .. Isolated community DNA (2 ng μL−1 ) was subjected to polymerase chain reaction (PCR) amplification of variable region V6 to V8 of SSU rRNA of bacteria and archaea by using barcoded primer pair 926f (AAA CTY AAA KGA ATT GAC GG), 1392r (ACG GGC GGT GTG TRC) in an iCycler® (Bio-Rad Laboratories Ltd., Meyerside Drive, ON, Canada) thermocycler under conditions: 95 °C for 3 min; 25 cycles of 95 °C for 30 s, 55 °C for 45 s, 72 °C for 90 s; and 72 °C for 10 min. DNA was purified using the QIAquick® PCR purification kit (Qiagen, Toronto, ON, Canada).

    Article Title: Childhood Adversity Impact on Gut Microbiota and Inflammatory Response to Stress During Pregnancy
    Article Snippet: .. DNA was isolated from approximately 200 mg stool using the MoBio PowerSoil® DNA Isolation Kit (Catalog # 12888–50). .. 16S sequencing was performed; primers annealing to the V1–V2 region of the 16S bacterial gene were used for amplification as described previously ( ).

    Article Title: Core Sulphate-Reducing Microorganisms in Metal-Removing Semi-Passive Biochemical Reactors and the Co-Occurrence of Methanogens
    Article Snippet: .. DNA Extraction, Amplification of SSU rRNA Genes and Pyrosequencing After homogenization of the sample under liquid N2 with a pestle and mortar, DNA was extracted from 0.5 g wet weight using a PowerSoil DNA isolation Kit (MoBio Laboratories Incorporation, Carlsbad, CA, USA, Cat No:12888-100). .. Isolated community DNA (2 ng μL−1 ) was subjected to polymerase chain reaction (PCR) amplification of variable region V6 to V8 of SSU rRNA of bacteria and archaea by using barcoded primer pair 926f (AAA CTY AAA KGA ATT GAC GG), 1392r (ACG GGC GGT GTG TRC) in an iCycler® (Bio-Rad Laboratories Ltd., Meyerside Drive, ON, Canada) thermocycler under conditions: 95 °C for 3 min; 25 cycles of 95 °C for 30 s, 55 °C for 45 s, 72 °C for 90 s; and 72 °C for 10 min. DNA was purified using the QIAquick® PCR purification kit (Qiagen, Toronto, ON, Canada).

    Article Title: The Microbial Community of a Passive Biochemical Reactor Treating Arsenic, Zinc, and Sulfate-Rich Seepage
    Article Snippet: .. The DNA was extracted using the Power Soil DNA isolation Kit (MoBio Laboratories Incorporation, Carlsbad, CA, USA, Cat No:12888-100) following the manufacturer’s instructions. .. DNA from adjacent 5-cm intervals was combined to yield 38 samples from all cores and all depths that were used for PCR amplification of the V6–V8 variable SSU rRNA region: 926f (5′ AAACTYAAAKGAATTGRCGG 3′) and 1392r (5′ ACGGGCGGTGTGTRC 3′) as described in detail in the Supplemental Material.

    Article Title: Boosting natural history research via metagenomic clean-up of crowdsourced feces
    Article Snippet: Lab work : DNA extraction (QIAGEN DNeasy Blood & Tissue kit) for multidimensional work of this nature, preferably sampling the interior of the sample. .. Kits such as the QIAGEN DNeasy PowerSoil kit if microbiome characterization is the focus.

    Article Title: STAT2 dependent Type I Interferon response promotes dysbiosis and luminal expansion of the enteric pathogen Salmonella Typhimurium
    Article Snippet: .. Microbiota analysis DNA from fecal contents of wild-type and Stat2 -/- infected mice was extracted using the PowerSoil DNA Isolation Kit (MoBio, 12888–50) according to manufacturer’s protocol. .. High quality isolated DNA was then submitted to SeqMatic for 16S rRNA V4 sequencing using the Illumina MiSeq platform.

    Article Title: Early-Onset Preeclampsia Is Associated With Gut Microbial Alterations in Antepartum and Postpartum Women
    Article Snippet: .. DNA Extraction and 16S rRNA Gene Sequencing The microbial genomic DNA was extracted according to the MOBIO PowerSoil® DNA Isolation Kit 12888-100 protocol, and DNA was stored at −80°C in Tris-EDTA buffer solution before use. .. To enable amplification of the V4 region of the 16S rRNA gene and add barcode sequences, unique fusion primers were designed based on the universal primers set, 515F (5′-GTGYCAGCMGCCGCGGTAA-3′) and 806R (5′-GGACTACNVGGGTWTCTAAT-3′), along with barcode sequences.

    Magnetic Beads:

    Article Title: Oral microbiome in HIV-associated periodontitis
    Article Snippet: 2.3 DNA extraction, 16 s rRNA gene amplification, and sequencing Genomic DNA from all samples was extracted utilizing the MoBio PowerSoil DNA Isolation Kit (Cat # 12888–50) and quantified via Quant-iT PicoGreen dsDNA Assay (Cat # P11496). .. [ ] PCR cleanup was performed using Agencourt AmpureXP magnetic beads (Cat # A63880).

    Isolation:

    Article Title: Core Sulphate-Reducing Microorganisms in Metal-Removing Semi-Passive Biochemical Reactors and the Co-Occurrence of Methanogens
    Article Snippet: After homogenization of the sample under liquid N2 with a pestle and mortar, DNA was extracted from 0.5 g wet weight using a PowerSoil DNA isolation Kit (MoBio Laboratories Incorporation, Carlsbad, CA, USA, Cat No:12888-100). .. Isolated community DNA (2 ng μL−1 ) was subjected to polymerase chain reaction (PCR) amplification of variable region V6 to V8 of SSU rRNA of bacteria and archaea by using barcoded primer pair 926f (AAA CTY AAA KGA ATT GAC GG), 1392r (ACG GGC GGT GTG TRC) in an iCycler® (Bio-Rad Laboratories Ltd., Meyerside Drive, ON, Canada) thermocycler under conditions: 95 °C for 3 min; 25 cycles of 95 °C for 30 s, 55 °C for 45 s, 72 °C for 90 s; and 72 °C for 10 min. DNA was purified using the QIAquick® PCR purification kit (Qiagen, Toronto, ON, Canada).

    Article Title: Childhood Adversity Impact on Gut Microbiota and Inflammatory Response to Stress During Pregnancy
    Article Snippet: .. DNA was isolated from approximately 200 mg stool using the MoBio PowerSoil® DNA Isolation Kit (Catalog # 12888–50). .. 16S sequencing was performed; primers annealing to the V1–V2 region of the 16S bacterial gene were used for amplification as described previously ( ).

    Article Title: Core Sulphate-Reducing Microorganisms in Metal-Removing Semi-Passive Biochemical Reactors and the Co-Occurrence of Methanogens
    Article Snippet: DNA Extraction, Amplification of SSU rRNA Genes and Pyrosequencing After homogenization of the sample under liquid N2 with a pestle and mortar, DNA was extracted from 0.5 g wet weight using a PowerSoil DNA isolation Kit (MoBio Laboratories Incorporation, Carlsbad, CA, USA, Cat No:12888-100). .. Isolated community DNA (2 ng μL−1 ) was subjected to polymerase chain reaction (PCR) amplification of variable region V6 to V8 of SSU rRNA of bacteria and archaea by using barcoded primer pair 926f (AAA CTY AAA KGA ATT GAC GG), 1392r (ACG GGC GGT GTG TRC) in an iCycler® (Bio-Rad Laboratories Ltd., Meyerside Drive, ON, Canada) thermocycler under conditions: 95 °C for 3 min; 25 cycles of 95 °C for 30 s, 55 °C for 45 s, 72 °C for 90 s; and 72 °C for 10 min. DNA was purified using the QIAquick® PCR purification kit (Qiagen, Toronto, ON, Canada).

    Article Title: STAT2 dependent Type I Interferon response promotes dysbiosis and luminal expansion of the enteric pathogen Salmonella Typhimurium
    Article Snippet: Microbiota analysis DNA from fecal contents of wild-type and Stat2 -/- infected mice was extracted using the PowerSoil DNA Isolation Kit (MoBio, 12888–50) according to manufacturer’s protocol. .. High quality isolated DNA was then submitted to SeqMatic for 16S rRNA V4 sequencing using the Illumina MiSeq platform.

    Purification:

    Article Title: Metagenomic Analysis of Microbial Community Compositions and Cold-Responsive Stress Genes in Selected Antarctic Lacustrine and Soil Ecosystems
    Article Snippet: DNA Extraction and Sequencing Purification of community DNA from LU_water and LU_sediment samples was carried out by using sterilized scalpels, separate pipettes, and separate fresh reagents to avoid cross contamination. .. The LU_sediment samples (1 g each) and the membrane filters were subjected to DNA extraction by using the MoBio PowerSoil® DNA Isolation Kit (MoBio Laboratories Inc., Carlsbad, CA, USA; cat # 12888–100).

    Article Title: High-throughput sequencing data and antibiotic resistance mechanisms of soil microbial communities in non-irrigated and irrigated soils with raw sewage in African cities
    Article Snippet: To collect the bacterial cells from the different soils, soil biomass purification was conducted according to Sentchilo et al. (2013) [ ]. .. Soil DNA was extracted using the DNeasy PowerSoil Kit (Qiagen, Germany) according to the manufacturer's instructions.

    Article Title: Core Sulphate-Reducing Microorganisms in Metal-Removing Semi-Passive Biochemical Reactors and the Co-Occurrence of Methanogens
    Article Snippet: After homogenization of the sample under liquid N2 with a pestle and mortar, DNA was extracted from 0.5 g wet weight using a PowerSoil DNA isolation Kit (MoBio Laboratories Incorporation, Carlsbad, CA, USA, Cat No:12888-100). .. Isolated community DNA (2 ng μL−1 ) was subjected to polymerase chain reaction (PCR) amplification of variable region V6 to V8 of SSU rRNA of bacteria and archaea by using barcoded primer pair 926f (AAA CTY AAA KGA ATT GAC GG), 1392r (ACG GGC GGT GTG TRC) in an iCycler® (Bio-Rad Laboratories Ltd., Meyerside Drive, ON, Canada) thermocycler under conditions: 95 °C for 3 min; 25 cycles of 95 °C for 30 s, 55 °C for 45 s, 72 °C for 90 s; and 72 °C for 10 min. DNA was purified using the QIAquick® PCR purification kit (Qiagen, Toronto, ON, Canada).

    Article Title: Childhood Adversity Impact on Gut Microbiota and Inflammatory Response to Stress During Pregnancy
    Article Snippet: DNA was isolated from approximately 200 mg stool using the MoBio PowerSoil® DNA Isolation Kit (Catalog # 12888–50). .. Purified products from the samples were pooled in equal amounts and sequenced using Illumina MiSeq; positive and negative controls were used ( ).

    Article Title: Core Sulphate-Reducing Microorganisms in Metal-Removing Semi-Passive Biochemical Reactors and the Co-Occurrence of Methanogens
    Article Snippet: DNA Extraction, Amplification of SSU rRNA Genes and Pyrosequencing After homogenization of the sample under liquid N2 with a pestle and mortar, DNA was extracted from 0.5 g wet weight using a PowerSoil DNA isolation Kit (MoBio Laboratories Incorporation, Carlsbad, CA, USA, Cat No:12888-100). .. Isolated community DNA (2 ng μL−1 ) was subjected to polymerase chain reaction (PCR) amplification of variable region V6 to V8 of SSU rRNA of bacteria and archaea by using barcoded primer pair 926f (AAA CTY AAA KGA ATT GAC GG), 1392r (ACG GGC GGT GTG TRC) in an iCycler® (Bio-Rad Laboratories Ltd., Meyerside Drive, ON, Canada) thermocycler under conditions: 95 °C for 3 min; 25 cycles of 95 °C for 30 s, 55 °C for 45 s, 72 °C for 90 s; and 72 °C for 10 min. DNA was purified using the QIAquick® PCR purification kit (Qiagen, Toronto, ON, Canada).

    Article Title: The Microbial Community of a Passive Biochemical Reactor Treating Arsenic, Zinc, and Sulfate-Rich Seepage
    Article Snippet: The DNA was extracted using the Power Soil DNA isolation Kit (MoBio Laboratories Incorporation, Carlsbad, CA, USA, Cat No:12888-100) following the manufacturer’s instructions. .. Purified PCR products were sent to the McGill University Innovation Centre (Montréal, QC, Canada) for pyrosequencing using a Roche GS-FLX Titanium Series sequencer.

    Polymerase Chain Reaction:

    Article Title: Alfalfa-containing diets alter luminal microbiota structure and short chain fatty acid sensing in the caecal mucosa of pigs
    Article Snippet: .. Bacterial DNA extraction, PCR amplification, and Illumina MiSeq sequencing The total genomic DNA was extracted from caecal digesta using Power Soil DNA Isolation Kit (12888–50; Mobio Technologies Inc., Vancouver, BC) following the manufacture recommendations. ..

    Article Title: Oral microbiome in HIV-associated periodontitis
    Article Snippet: 2.3 DNA extraction, 16 s rRNA gene amplification, and sequencing Genomic DNA from all samples was extracted utilizing the MoBio PowerSoil DNA Isolation Kit (Cat # 12888–50) and quantified via Quant-iT PicoGreen dsDNA Assay (Cat # P11496). .. The 16 s V4 region was amplified through 30 cycles PCR with an F515 locus-specific primer (5′-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG-3′) and an 806R-locus specific primer (5′-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG-3′) both with compatible overhang regions for index attachment.

    Article Title: Core Sulphate-Reducing Microorganisms in Metal-Removing Semi-Passive Biochemical Reactors and the Co-Occurrence of Methanogens
    Article Snippet: After homogenization of the sample under liquid N2 with a pestle and mortar, DNA was extracted from 0.5 g wet weight using a PowerSoil DNA isolation Kit (MoBio Laboratories Incorporation, Carlsbad, CA, USA, Cat No:12888-100). .. Isolated community DNA (2 ng μL−1 ) was subjected to polymerase chain reaction (PCR) amplification of variable region V6 to V8 of SSU rRNA of bacteria and archaea by using barcoded primer pair 926f (AAA CTY AAA KGA ATT GAC GG), 1392r (ACG GGC GGT GTG TRC) in an iCycler® (Bio-Rad Laboratories Ltd., Meyerside Drive, ON, Canada) thermocycler under conditions: 95 °C for 3 min; 25 cycles of 95 °C for 30 s, 55 °C for 45 s, 72 °C for 90 s; and 72 °C for 10 min. DNA was purified using the QIAquick® PCR purification kit (Qiagen, Toronto, ON, Canada).

    Article Title: Core Sulphate-Reducing Microorganisms in Metal-Removing Semi-Passive Biochemical Reactors and the Co-Occurrence of Methanogens
    Article Snippet: DNA Extraction, Amplification of SSU rRNA Genes and Pyrosequencing After homogenization of the sample under liquid N2 with a pestle and mortar, DNA was extracted from 0.5 g wet weight using a PowerSoil DNA isolation Kit (MoBio Laboratories Incorporation, Carlsbad, CA, USA, Cat No:12888-100). .. Isolated community DNA (2 ng μL−1 ) was subjected to polymerase chain reaction (PCR) amplification of variable region V6 to V8 of SSU rRNA of bacteria and archaea by using barcoded primer pair 926f (AAA CTY AAA KGA ATT GAC GG), 1392r (ACG GGC GGT GTG TRC) in an iCycler® (Bio-Rad Laboratories Ltd., Meyerside Drive, ON, Canada) thermocycler under conditions: 95 °C for 3 min; 25 cycles of 95 °C for 30 s, 55 °C for 45 s, 72 °C for 90 s; and 72 °C for 10 min. DNA was purified using the QIAquick® PCR purification kit (Qiagen, Toronto, ON, Canada).

    Article Title: The Microbial Community of a Passive Biochemical Reactor Treating Arsenic, Zinc, and Sulfate-Rich Seepage
    Article Snippet: Clone libraries for the middle section (20–30 cm depth) of each of the nine cores were produced for Archaea and bacteria using primers targeting the archaeal domain A4F (5′-TCCGGTTGATCCTGCCRG) and U1492R (5′-GGTTACCTTGTTACGACTT) and the bacterial domain B27F (5′-AGAGTTTGATCCTGGCTCAG) and U1492R (5′-GGTTACCTTAGTTACGACTT), respectively, using gradient PCR methods described in detail in the Supplemental Material. .. The DNA was extracted using the Power Soil DNA isolation Kit (MoBio Laboratories Incorporation, Carlsbad, CA, USA, Cat No:12888-100) following the manufacturer’s instructions.

    Article Title: Early-Onset Preeclampsia Is Associated With Gut Microbial Alterations in Antepartum and Postpartum Women
    Article Snippet: DNA Extraction and 16S rRNA Gene Sequencing The microbial genomic DNA was extracted according to the MOBIO PowerSoil® DNA Isolation Kit 12888-100 protocol, and DNA was stored at −80°C in Tris-EDTA buffer solution before use. .. PCR mixtures contained 1 μL of each forward and reverse primer (10 μM), 1 μL of template DNA, 4 μL of dNTPs (2.5 mM), 5 μL of 10 × EasyPfu Buffer, 1 μL of Easy Pfu DNA Polymerase (2.5 U/μL), and 1 μL of double distilled water in a 50 μL reaction volume.

    Mouse Assay:

    Article Title: STAT2 dependent Type I Interferon response promotes dysbiosis and luminal expansion of the enteric pathogen Salmonella Typhimurium
    Article Snippet: .. Microbiota analysis DNA from fecal contents of wild-type and Stat2 -/- infected mice was extracted using the PowerSoil DNA Isolation Kit (MoBio, 12888–50) according to manufacturer’s protocol. .. High quality isolated DNA was then submitted to SeqMatic for 16S rRNA V4 sequencing using the Illumina MiSeq platform.

    Agarose Gel Electrophoresis:

    Article Title: Metagenomic Analysis of Microbial Community Compositions and Cold-Responsive Stress Genes in Selected Antarctic Lacustrine and Soil Ecosystems
    Article Snippet: The LU_sediment samples (1 g each) and the membrane filters were subjected to DNA extraction by using the MoBio PowerSoil® DNA Isolation Kit (MoBio Laboratories Inc., Carlsbad, CA, USA; cat # 12888–100). .. The quality and concentration of the pooled DNA from each water and sediment sample was determined by using a Lambda II spectrophotometer (Perkin Elmer, Norwalk, Conn.) followed by agarose gel electrophoresis (1% wt/vol agarose in 1X Tris-Acetate-EDTA (TAE) buffer, pH 7.8) [ ].

    Article Title: Core Sulphate-Reducing Microorganisms in Metal-Removing Semi-Passive Biochemical Reactors and the Co-Occurrence of Methanogens
    Article Snippet: After homogenization of the sample under liquid N2 with a pestle and mortar, DNA was extracted from 0.5 g wet weight using a PowerSoil DNA isolation Kit (MoBio Laboratories Incorporation, Carlsbad, CA, USA, Cat No:12888-100). .. DNA concentrations were measured on a NanoDrop® ND-2000 UV-Vis Spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA) and by running 1 µL of the PCR product on a 1% agarose gel.

    Article Title: Core Sulphate-Reducing Microorganisms in Metal-Removing Semi-Passive Biochemical Reactors and the Co-Occurrence of Methanogens
    Article Snippet: DNA Extraction, Amplification of SSU rRNA Genes and Pyrosequencing After homogenization of the sample under liquid N2 with a pestle and mortar, DNA was extracted from 0.5 g wet weight using a PowerSoil DNA isolation Kit (MoBio Laboratories Incorporation, Carlsbad, CA, USA, Cat No:12888-100). .. DNA concentrations were measured on a NanoDrop® ND-2000 UV-Vis Spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA) and by running 1 µL of the PCR product on a 1% agarose gel.

    Article Title: Early-Onset Preeclampsia Is Associated With Gut Microbial Alterations in Antepartum and Postpartum Women
    Article Snippet: DNA Extraction and 16S rRNA Gene Sequencing The microbial genomic DNA was extracted according to the MOBIO PowerSoil® DNA Isolation Kit 12888-100 protocol, and DNA was stored at −80°C in Tris-EDTA buffer solution before use. .. Thermal cycling consisted of an initial denaturation step at 95°C for 5 min, followed by 30 cycles of denaturation at 94°C for 30 s, annealing at 60°C for 30 s, and extension at 72°C for 40 s, with a final extension step at 72°C for 4 min. Amplicons from each sample were run on an agarose gel.

    Homogenization:

    Article Title: Systematic Bias Introduced by Genomic DNA Template Dilution in 16S rRNA Gene-Targeted Microbiota Profiling in Human Stool Homogenates
    Article Snippet: Paragraph title: Sample collection and homogenization. ... The contents of one PowerBead tube (Mo Bio Laboratories, Inc.; catalog no. 12888-50-PBT) and 60 μl of solution C1 (Mo Bio Laboratories, Inc.; catalog no. 12888-50-1) were added to each aliquot.

    Article Title: Core Sulphate-Reducing Microorganisms in Metal-Removing Semi-Passive Biochemical Reactors and the Co-Occurrence of Methanogens
    Article Snippet: .. After homogenization of the sample under liquid N2 with a pestle and mortar, DNA was extracted from 0.5 g wet weight using a PowerSoil DNA isolation Kit (MoBio Laboratories Incorporation, Carlsbad, CA, USA, Cat No:12888-100). .. Isolated community DNA (2 ng μL−1 ) was subjected to polymerase chain reaction (PCR) amplification of variable region V6 to V8 of SSU rRNA of bacteria and archaea by using barcoded primer pair 926f (AAA CTY AAA KGA ATT GAC GG), 1392r (ACG GGC GGT GTG TRC) in an iCycler® (Bio-Rad Laboratories Ltd., Meyerside Drive, ON, Canada) thermocycler under conditions: 95 °C for 3 min; 25 cycles of 95 °C for 30 s, 55 °C for 45 s, 72 °C for 90 s; and 72 °C for 10 min. DNA was purified using the QIAquick® PCR purification kit (Qiagen, Toronto, ON, Canada).

    Article Title: Core Sulphate-Reducing Microorganisms in Metal-Removing Semi-Passive Biochemical Reactors and the Co-Occurrence of Methanogens
    Article Snippet: .. DNA Extraction, Amplification of SSU rRNA Genes and Pyrosequencing After homogenization of the sample under liquid N2 with a pestle and mortar, DNA was extracted from 0.5 g wet weight using a PowerSoil DNA isolation Kit (MoBio Laboratories Incorporation, Carlsbad, CA, USA, Cat No:12888-100). .. Isolated community DNA (2 ng μL−1 ) was subjected to polymerase chain reaction (PCR) amplification of variable region V6 to V8 of SSU rRNA of bacteria and archaea by using barcoded primer pair 926f (AAA CTY AAA KGA ATT GAC GG), 1392r (ACG GGC GGT GTG TRC) in an iCycler® (Bio-Rad Laboratories Ltd., Meyerside Drive, ON, Canada) thermocycler under conditions: 95 °C for 3 min; 25 cycles of 95 °C for 30 s, 55 °C for 45 s, 72 °C for 90 s; and 72 °C for 10 min. DNA was purified using the QIAquick® PCR purification kit (Qiagen, Toronto, ON, Canada).

    Preserving:

    Article Title: Boosting natural history research via metagenomic clean-up of crowdsourced feces
    Article Snippet: When such preservation is not possible, a 2-step preservation method can be used: first, storage in ethanol and then desiccation in silica after 1 day [ ]. .. Kits such as the QIAGEN DNeasy PowerSoil kit if microbiome characterization is the focus.

    Spectrophotometry:

    Article Title: Metagenomic Analysis of Microbial Community Compositions and Cold-Responsive Stress Genes in Selected Antarctic Lacustrine and Soil Ecosystems
    Article Snippet: The LU_sediment samples (1 g each) and the membrane filters were subjected to DNA extraction by using the MoBio PowerSoil® DNA Isolation Kit (MoBio Laboratories Inc., Carlsbad, CA, USA; cat # 12888–100). .. The quality and concentration of the pooled DNA from each water and sediment sample was determined by using a Lambda II spectrophotometer (Perkin Elmer, Norwalk, Conn.) followed by agarose gel electrophoresis (1% wt/vol agarose in 1X Tris-Acetate-EDTA (TAE) buffer, pH 7.8) [ ].

    Article Title: Core Sulphate-Reducing Microorganisms in Metal-Removing Semi-Passive Biochemical Reactors and the Co-Occurrence of Methanogens
    Article Snippet: After homogenization of the sample under liquid N2 with a pestle and mortar, DNA was extracted from 0.5 g wet weight using a PowerSoil DNA isolation Kit (MoBio Laboratories Incorporation, Carlsbad, CA, USA, Cat No:12888-100). .. DNA concentrations were measured on a NanoDrop® ND-2000 UV-Vis Spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA) and by running 1 µL of the PCR product on a 1% agarose gel.

    Article Title: Core Sulphate-Reducing Microorganisms in Metal-Removing Semi-Passive Biochemical Reactors and the Co-Occurrence of Methanogens
    Article Snippet: DNA Extraction, Amplification of SSU rRNA Genes and Pyrosequencing After homogenization of the sample under liquid N2 with a pestle and mortar, DNA was extracted from 0.5 g wet weight using a PowerSoil DNA isolation Kit (MoBio Laboratories Incorporation, Carlsbad, CA, USA, Cat No:12888-100). .. DNA concentrations were measured on a NanoDrop® ND-2000 UV-Vis Spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA) and by running 1 µL of the PCR product on a 1% agarose gel.

    Article Title: Axiom Microbiome Array, the next generation microarray for high-throughput pathogen and microbiome analysis
    Article Snippet: DNA concentration was determined by Qubit spectrophotometer and the DNA genome copy numbers per test sample were calculated based on the genomic size of the two bacteria. .. Soil was collected from locations in San Francisco as described previously [ ] and DNA was extracted using the MoBio PowerSoil extraction kit (Catalog number: 12888–50).

    Sampling:

    Article Title: Childhood Adversity Impact on Gut Microbiota and Inflammatory Response to Stress During Pregnancy
    Article Snippet: Paragraph title: 2.3. Stool sampling and sequencing. ... DNA was isolated from approximately 200 mg stool using the MoBio PowerSoil® DNA Isolation Kit (Catalog # 12888–50).

    Article Title: Boosting natural history research via metagenomic clean-up of crowdsourced feces
    Article Snippet: Lab work : DNA extraction (QIAGEN DNeasy Blood & Tissue kit) for multidimensional work of this nature, preferably sampling the interior of the sample. .. Kits such as the QIAGEN DNeasy PowerSoil kit if microbiome characterization is the focus.

    Produced:

    Article Title: The Microbial Community of a Passive Biochemical Reactor Treating Arsenic, Zinc, and Sulfate-Rich Seepage
    Article Snippet: Clone libraries for the middle section (20–30 cm depth) of each of the nine cores were produced for Archaea and bacteria using primers targeting the archaeal domain A4F (5′-TCCGGTTGATCCTGCCRG) and U1492R (5′-GGTTACCTTGTTACGACTT) and the bacterial domain B27F (5′-AGAGTTTGATCCTGGCTCAG) and U1492R (5′-GGTTACCTTAGTTACGACTT), respectively, using gradient PCR methods described in detail in the Supplemental Material. .. The DNA was extracted using the Power Soil DNA isolation Kit (MoBio Laboratories Incorporation, Carlsbad, CA, USA, Cat No:12888-100) following the manufacturer’s instructions.

    Concentration Assay:

    Article Title: Metagenomic Analysis of Microbial Community Compositions and Cold-Responsive Stress Genes in Selected Antarctic Lacustrine and Soil Ecosystems
    Article Snippet: The LU_sediment samples (1 g each) and the membrane filters were subjected to DNA extraction by using the MoBio PowerSoil® DNA Isolation Kit (MoBio Laboratories Inc., Carlsbad, CA, USA; cat # 12888–100). .. The quality and concentration of the pooled DNA from each water and sediment sample was determined by using a Lambda II spectrophotometer (Perkin Elmer, Norwalk, Conn.) followed by agarose gel electrophoresis (1% wt/vol agarose in 1X Tris-Acetate-EDTA (TAE) buffer, pH 7.8) [ ].

    Article Title: High-throughput sequencing data and antibiotic resistance mechanisms of soil microbial communities in non-irrigated and irrigated soils with raw sewage in African cities
    Article Snippet: Soil DNA was extracted using the DNeasy PowerSoil Kit (Qiagen, Germany) according to the manufacturer's instructions. .. DNA concentration was determined by using the Quant-iT PicoGreen dsDNA Assay Kit, and the Qubit™ 3.0 Fluorometer (Qubit, Life Technologies, USA).

    Article Title: Axiom Microbiome Array, the next generation microarray for high-throughput pathogen and microbiome analysis
    Article Snippet: DNA concentration was determined by Qubit spectrophotometer and the DNA genome copy numbers per test sample were calculated based on the genomic size of the two bacteria. .. Soil was collected from locations in San Francisco as described previously [ ] and DNA was extracted using the MoBio PowerSoil extraction kit (Catalog number: 12888–50).

    High Throughput Screening Assay:

    Article Title: High-throughput sequencing data and antibiotic resistance mechanisms of soil microbial communities in non-irrigated and irrigated soils with raw sewage in African cities
    Article Snippet: Soil DNA was extracted using the DNeasy PowerSoil Kit (Qiagen, Germany) according to the manufacturer's instructions. .. Six DNA samples representative of the three cities were sent to Edinburgh Genomics for high-throughput sequencing.

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    Qiagen solution c4
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