h46a  (ATCC)


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    ATCC h46a
    H46a, supplied by ATCC, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/h46a/product/ATCC
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    h46a - by Bioz Stars, 2022-10
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    ATCC strain h46a
    SDS-PAGE: Purification of SK from <t>H46a</t> culture by reducing agent(lane 1); Protein MW Marker. (lane 2); Standard SK. (lane 3 4); H46a culture. (lane 5); 2 mg methanol extract treated with 200 mM DTT Aldrithiol. (lane 6); 2 mg methanol extract treated with 300 mM DTT Aldrithiol
    Strain H46a, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/strain h46a/product/ATCC
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    strain h46a - by Bioz Stars, 2022-10
    93/100 stars
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    SDS-PAGE: Purification of SK from H46a culture by reducing agent(lane 1); Protein MW Marker. (lane 2); Standard SK. (lane 3 4); H46a culture. (lane 5); 2 mg methanol extract treated with 200 mM DTT Aldrithiol. (lane 6); 2 mg methanol extract treated with 300 mM DTT Aldrithiol

    Journal: Iranian Journal of Microbiology

    Article Title: Fermentation, fractionation and purification of streptokinase by chemical reduction method

    doi:

    Figure Lengend Snippet: SDS-PAGE: Purification of SK from H46a culture by reducing agent(lane 1); Protein MW Marker. (lane 2); Standard SK. (lane 3 4); H46a culture. (lane 5); 2 mg methanol extract treated with 200 mM DTT Aldrithiol. (lane 6); 2 mg methanol extract treated with 300 mM DTT Aldrithiol

    Article Snippet: MATERIALS AND METHODS The bacterial strain and materials used in the present study includes; S. equisimilis group C, strain H46A (ATCC 12449, USA), Todd Hewitt Broth (THB, HiMEDIA Laboratories), Brain Heart Infusion (BHI, USA), Trypticase Soy Agar (TSA, BBL, USA) Lysine monohydrochloride (Sigma Chemical, USA), Hexyl resorcinol (Merck, Germany), 3-amino-n-caproic acid (EACA, Sigma Chemical, USA), Cyanogen bromide-activated Sepharose 4B (Sigma Chemical, USA), Chromogenic substrate S-2251 (Chromogenix laboratories, Italy), Dithiothreitol (Sigma Chemical, USA), Aldrithiol-2 (Sigma Chemical, USA), Salts, acids and bases for buffers (Merck, Germany).

    Techniques: SDS Page, Purification, Marker