plasmid midi kit  (Qiagen)


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    Name:
    QIAGEN Plasmid Midi Kit
    Description:
    For purification of up to 10 mg transfection grade plasmid or cosmid DNA Kit contents Qiagen Plasmid Midi Kit 25 preps 25 to 100mL Culture Volume Manual Centrifugation Processing 150 min Time Run Anion exchange Technology 1 Sample Run For Purification of up to 100g Transfection grade Plasmid or Cosmid DNA Ideal for Cloning Sequencing Capillary Sequencing In vitro Transcription Includes 25 Qiagen to tip 100 Reagents Buffers Benefits Purity equivalent to 2 x CsCl gradient centrifugation High yields of plasmid DNA Cost effective preparations LyseBlue for optimum lysis and maximum DNA yie
    Catalog Number:
    12143
    Price:
    288
    Category:
    QIAGEN Plasmid Kits
    Buy from Supplier


    Structured Review

    Qiagen plasmid midi kit
    QIAGEN Plasmid Midi Kit
    For purification of up to 10 mg transfection grade plasmid or cosmid DNA Kit contents Qiagen Plasmid Midi Kit 25 preps 25 to 100mL Culture Volume Manual Centrifugation Processing 150 min Time Run Anion exchange Technology 1 Sample Run For Purification of up to 100g Transfection grade Plasmid or Cosmid DNA Ideal for Cloning Sequencing Capillary Sequencing In vitro Transcription Includes 25 Qiagen to tip 100 Reagents Buffers Benefits Purity equivalent to 2 x CsCl gradient centrifugation High yields of plasmid DNA Cost effective preparations LyseBlue for optimum lysis and maximum DNA yie
    https://www.bioz.com/result/plasmid midi kit/product/Qiagen
    Average 90 stars, based on 1225 article reviews
    Price from $9.99 to $1999.99
    plasmid midi kit - by Bioz Stars, 2020-01
    90/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Mrg15 stimulates Ash1 H3K36 methyltransferase activity and facilitates Ash1 Trithorax group protein function in Drosophila
    Article Snippet: Ash1 R1288A gRNA and donor plasmids were purified using a Qiagen Plasmid Midi Kit (#12145) and introduced into nos-Cas9 embryos using standard procedures. .. To make the UAST-ash1 , UAST-mrg15 , UAST-nurf55 and UAST-mrg15:nurf55 constructs, fragments were subcloned into an attB-UAST vector using ClonExpress II One Step Cloning Kit (Vazyme, C112).

    Article Title: Three-dimensional modeling of and ligand docking to vitamin D receptor ligand binding domain
    Article Snippet: .. The cDNAs of the clones were purified with a Qiagen Plasmid Midi-Kit (Qiagen, Chatsworth, CA). .. Each mutant cDNA of clones was sequenced completely to ensure that no other base changes were produced (by cycle sequencing).

    Article Title: Paternal Origin of FGFR2 Mutations in Sporadic Cases of Crouzon Syndrome and Pfeiffer Syndrome
    Article Snippet: The 1,663-bp PCR product was cloned by use of the TA Cloning Kit (Invitrogen). .. Plasmid DNA was isolated by use of the Qiagen Plasmid Midi-Kit.

    Article Title: Haplotype Analysis of the Pre-harvest Sprouting Resistance Locus Phs-A1 Reveals a Causal Role of TaMKK3-A in Global Germplasm
    Article Snippet: This identified two positive clones for PM19-A1 (TaaCsp4AL037H11 and TaaCsp4AL172K12) and three positive clones for TaMKK3-A (TaaCsp4AL032F12, TaaCsp4AL012P14 and TaaCsp4AL002F16; Supplementary Table ). .. DNA of the BACs was extracted using the Qiagen Plasmid Midi Kit (Qiagen, Cat. No. 12143).

    Article Title: Condensins are Required for Maintenance of Nuclear Architecture
    Article Snippet: .. BAC clones were cultured and DNA was purified using QIAGEN Plasmid Midi Kit (Qiagen 12143, Hilden, Germany). .. Purified BAC DNA was amplified using Whole genome amplification kit (Sigma-Aldrich WGA1), 20 µg of amplified DNA was then digested using a cocktail of AluI, Rsa, MseI, MspI, HaeIII, and BfuCl (New England BioLabs, Ipswich, MA, USA) overnight at 37 °C, ethanol precipitated, and resuspended in 36 µL ddH2 O. DNA was denatured at 100 °C for 1 min, and then 3'-end-labeled with unmodified aminoallyl dUTP and terminal deoxynucleotidyl transferase (Roche, Mannheim, Germany).

    Article Title: Assignment of Chinook Salmon (Oncorhynchus tshawytscha) Linkage Groups to Specific Chromosomes Reveals a Karyotype with Multiple Rearrangements of the Chromosome Arms of Rainbow Trout (Oncorhynchus mykiss)
    Article Snippet: .. BAC DNA was isolated from clones containing a microsatellite marker with a known position in the rainbow trout linkage map using the QIAGEN Plasmid Midi Kit (catalog #12143) following the manufacturer’s protocol. .. BAC DNA was labeled with either spectrum orange (Vysis) or digoxigenin (Roche), as recommended by the manufacturers.

    Article Title: Ovaries absent links dLsd1 to HP1a for local H3K4 demethylation required for heterochromatic gene silencing
    Article Snippet: Ova cDNA was cloned into UASP-λN and UASP-lacI (gifts from Julius Brennecke, Institute of Molecular Biotechnology) to generate the UASP-λN-ova and UASP-lacI-ova transgenes respectively. .. All the plasmids were purified using a Qiagen Plasmid Midi Kit (#12145) and the DNA sequencing verified plasmids were introduced into embryos using either P-element or nos-phiC31 system to generate transgenic flies according to a standard procedure.

    Centrifugation:

    Article Title: Assignment of Chinook Salmon (Oncorhynchus tshawytscha) Linkage Groups to Specific Chromosomes Reveals a Karyotype with Multiple Rearrangements of the Chromosome Arms of Rainbow Trout (Oncorhynchus mykiss)
    Article Snippet: Cells were collected by centrifugation and re-suspended in 0.075 M KCl for 30 min, then fixed in 3:1 methanol:acetic acid. .. BAC DNA was isolated from clones containing a microsatellite marker with a known position in the rainbow trout linkage map using the QIAGEN Plasmid Midi Kit (catalog #12143) following the manufacturer’s protocol.

    Amplification:

    Article Title: Role of the FeoB Protein and Siderophore in Promoting Virulence of Xanthomonas oryzae pv. oryzae on Rice ▿
    Article Snippet: Plasmid DNA was isolated by the alkaline lysis method ( ) or by using a Qiagen plasmid midi-kit. .. Phusion polymerase (Finnzymes) was used for PCR amplification in applications that required high-fidelity DNA synthesis (such as for complementation analysis) while Taq polymerase was used for all other applications.

    Article Title: ALTHEA Gold Libraries™: antibody libraries for therapeutic antibody discovery
    Article Snippet: .. Amplicon preparation and NGS data generation Plasmid DNA from PLs, FLs and SLs were purified using QIAGEN Plasmid Midi Kit (Cat No.: 12143) and used as templates to generate two amplicons of approximately 300 bps. .. The PCR reactions were performed as follows: 5 min start at 95°C followed by 10 cycles of 1 min at 95°C, 1 min at 67°C, 1 min at 72°C and terminated by a 10-min extension at 72°C.

    Article Title: Paternal Origin of FGFR2 Mutations in Sporadic Cases of Crouzon Syndrome and Pfeiffer Syndrome
    Article Snippet: The upstream intron (intron IIIb) of exon IIIc (also referred to as “exon 10” or “exon B”) was PCR amplified by use of primers 1 and 2 (annealing at 54°C for 1 min and extension at 72°C for 4 min per cycle [35 cycles]) ( ; ). .. Plasmid DNA was isolated by use of the Qiagen Plasmid Midi-Kit.

    Article Title: Regional Spread of Pseudomonas aeruginosa ST357 Producing IMP-7 Metallo-?-Lactamase in Central Europe ▿
    Article Snippet: The regions were amplified in two parts with primers specific for bla IMP genes ( ) and integronic conserved segments, 5′-CS and 3′-CS ( ); the 5′ parts were amplified with primers 5CS and Imp-R, whereas the 3′ parts were amplified with primers Imp-F and 3CS. .. Late-logarithmic cultures of the isolates were subjected to a plasmid purification procedure with the Qiagen plasmid midi-kit (Qiagen, Hilden, Germany).

    Article Title: Survivin Expression and Prognostic Significance in Pediatric Malignant Peripheral Nerve Sheath Tumors (MPNST)
    Article Snippet: The BAC probe was prepared from bacterial cultures using Qiagen-Plasmid Midi kit (Qiagen GmnH, Dϋsseldorf, Germany) and labelled by nick translation with SpectrumOrange-dUTP (Visys). .. Before being used for the analysis of tumor samples, the probe was assessed by PCR with primers specific for survivin gene (primer pair For 5′- GTG AAC GGA TAC CTC TCT ATA TGC TG-3′ and Rev 5′-CTG ACT ATC ACC GTT ACC AGA ACT G-3′ and the following conditions: denaturation 1 min at 94°C, annealing 2 min at 59°C and extension 3 min at 72°C for 35 cycles; the expected length of the amplified DNA was 949 bp), and by FISH on normal metaphases from PHA-stimulated peripheral blood mononuclear cells to check adequacy and consistency of hybridization signal.

    Article Title: Condensins are Required for Maintenance of Nuclear Architecture
    Article Snippet: BAC clones were cultured and DNA was purified using QIAGEN Plasmid Midi Kit (Qiagen 12143, Hilden, Germany). .. Purified BAC DNA was amplified using Whole genome amplification kit (Sigma-Aldrich WGA1), 20 µg of amplified DNA was then digested using a cocktail of AluI, Rsa, MseI, MspI, HaeIII, and BfuCl (New England BioLabs, Ipswich, MA, USA) overnight at 37 °C, ethanol precipitated, and resuspended in 36 µL ddH2 O. DNA was denatured at 100 °C for 1 min, and then 3'-end-labeled with unmodified aminoallyl dUTP and terminal deoxynucleotidyl transferase (Roche, Mannheim, Germany).

    DNA Synthesis:

    Article Title: Role of the FeoB Protein and Siderophore in Promoting Virulence of Xanthomonas oryzae pv. oryzae on Rice ▿
    Article Snippet: Plasmid DNA was isolated by the alkaline lysis method ( ) or by using a Qiagen plasmid midi-kit. .. Phusion polymerase (Finnzymes) was used for PCR amplification in applications that required high-fidelity DNA synthesis (such as for complementation analysis) while Taq polymerase was used for all other applications.

    High Throughput Screening Assay:

    Article Title: Haplotype Analysis of the Pre-harvest Sprouting Resistance Locus Phs-A1 Reveals a Causal Role of TaMKK3-A in Global Germplasm
    Article Snippet: Using the high-throughput BAC screening approach described by , a sequence database made from a three-dimensional pool of BAC clones comprising the Minimum Tilling Path (MTP) was searched for the sequences of PM19-A1 and TaMKK3-A . .. DNA of the BACs was extracted using the Qiagen Plasmid Midi Kit (Qiagen, Cat. No. 12143).

    TA Cloning:

    Article Title: Paternal Origin of FGFR2 Mutations in Sporadic Cases of Crouzon Syndrome and Pfeiffer Syndrome
    Article Snippet: The 1,663-bp PCR product was cloned by use of the TA Cloning Kit (Invitrogen). .. Plasmid DNA was isolated by use of the Qiagen Plasmid Midi-Kit.

    Construct:

    Article Title: Mrg15 stimulates Ash1 H3K36 methyltransferase activity and facilitates Ash1 Trithorax group protein function in Drosophila
    Article Snippet: Ash1 R1288A gRNA and donor plasmids were purified using a Qiagen Plasmid Midi Kit (#12145) and introduced into nos-Cas9 embryos using standard procedures. .. To make the UAST-ash1 , UAST-mrg15 , UAST-nurf55 and UAST-mrg15:nurf55 constructs, fragments were subcloned into an attB-UAST vector using ClonExpress II One Step Cloning Kit (Vazyme, C112).

    Article Title: Purification and Characterization of a Second Immunoreactive Mannoprotein from Cryptococcus neoformans That Stimulates T-Cell Responses
    Article Snippet: A cDNA library of C. neoformans B3501 constructed in the Uni-Zap XR vector (Stratagene) has been previously described ( ). .. Phagemid DNA was purified by using the Qiagen plasmid Midi-kit (Qiagen, Valencia, Calif.).

    Article Title: Three-dimensional modeling of and ligand docking to vitamin D receptor ligand binding domain
    Article Snippet: The human VDR expression vector pCMX-hVDR was constructed as described ( ) and was used as a template for site-directed mutagenesis. .. The cDNAs of the clones were purified with a Qiagen Plasmid Midi-Kit (Qiagen, Chatsworth, CA).

    Article Title: Effects of optional structural elements, including two alternative amino termini and a new splicing cassette IV, on the function of the sodium–bicarbonate cotransporter NBCn1 (SLC4A7)
    Article Snippet: The resulting construct was transformed into ABLE C Competent Cells (Cat. No. 200171, Agilent Technologies, Santa Clara, CA, USA). .. In total, 250 ml of bacterial culture was grown from one single colony and plasmids containing the cDNA of human NBCn1 were isolated with a QIAGEN Plasmid Midi Kit (Cat. No. 12143, QIAGEN, Germantown, MD, USA) according to the manufacturer's instructions.

    Article Title: Ovaries absent links dLsd1 to HP1a for local H3K4 demethylation required for heterochromatic gene silencing
    Article Snippet: The GFP-ova construct was obtained using Gateway cloning technology (Invitrogen) and pUGW (DGRC1283) vector. .. All the plasmids were purified using a Qiagen Plasmid Midi Kit (#12145) and the DNA sequencing verified plasmids were introduced into embryos using either P-element or nos-phiC31 system to generate transgenic flies according to a standard procedure.

    Article Title: Radiosensitization by PARP Inhibition in DNA Repair Proficient and Deficient Tumor Cells: Proliferative Recovery in Senescent Cells
    Article Snippet: .. ATG5 and ATG7 silencing shCon, shATG5 and shATG7 plasmid constructs were isolated (Qiagen-plasmid midi kit) using bacterial stocks (Sigma-Aldrich). .. Plasmid constructs were packaged into lentiviral particles using HEK 239T cells and a packaging mixture composed of Lipofectamine (Invitrogen, 11668–019), psPAX2 and pMD2.G packaging constructs (Addgene, 12260, 12259).

    Incubation:

    Article Title: Radiosensitization by PARP Inhibition in DNA Repair Proficient and Deficient Tumor Cells: Proliferative Recovery in Senescent Cells
    Article Snippet: HCT116 Ligase IV-deficient and Ligase IV proficient cells lines were maintained as subconfluent cultures in RPMI 1640 medium with 5% fetal bovine serum, 5% bovine calf serum, 2 mM L-glutamine, and penicillin/ (GIBCO Life Technologies, Gaithersburg, MD) and incubated at 37°C, 5% CO2 , in a humidified environment. .. ATG5 and ATG7 silencing shCon, shATG5 and shATG7 plasmid constructs were isolated (Qiagen-plasmid midi kit) using bacterial stocks (Sigma-Aldrich).

    Formalin-fixed Paraffin-Embedded:

    Article Title: Survivin Expression and Prognostic Significance in Pediatric Malignant Peripheral Nerve Sheath Tumors (MPNST)
    Article Snippet: The BAC probe was prepared from bacterial cultures using Qiagen-Plasmid Midi kit (Qiagen GmnH, Dϋsseldorf, Germany) and labelled by nick translation with SpectrumOrange-dUTP (Visys). .. FISH was performed on 4 µm FFPE sections as previously described .

    Infection:

    Article Title: Radiosensitization by PARP Inhibition in DNA Repair Proficient and Deficient Tumor Cells: Proliferative Recovery in Senescent Cells
    Article Snippet: ATG5 and ATG7 silencing shCon, shATG5 and shATG7 plasmid constructs were isolated (Qiagen-plasmid midi kit) using bacterial stocks (Sigma-Aldrich). .. Infected cells were then maintained with the selection marker, puromycin (2 µg/mL) throughout the course of the study.

    Expressing:

    Article Title: Three-dimensional modeling of and ligand docking to vitamin D receptor ligand binding domain
    Article Snippet: The human VDR expression vector pCMX-hVDR was constructed as described ( ) and was used as a template for site-directed mutagenesis. .. The cDNAs of the clones were purified with a Qiagen Plasmid Midi-Kit (Qiagen, Chatsworth, CA).

    Article Title: Effects of optional structural elements, including two alternative amino termini and a new splicing cassette IV, on the function of the sodium–bicarbonate cotransporter NBCn1 (SLC4A7)
    Article Snippet: The human NBCn1 variants were enhanced green fluorescent protein (EGFP)-tagged at the Ct, as described previously, starting from a pGH19 expression vector containing cDNA encoding EGFP ( ). .. In total, 250 ml of bacterial culture was grown from one single colony and plasmids containing the cDNA of human NBCn1 were isolated with a QIAGEN Plasmid Midi Kit (Cat. No. 12143, QIAGEN, Germantown, MD, USA) according to the manufacturer's instructions.

    Whole Genome Amplification:

    Article Title: Condensins are Required for Maintenance of Nuclear Architecture
    Article Snippet: BAC clones were cultured and DNA was purified using QIAGEN Plasmid Midi Kit (Qiagen 12143, Hilden, Germany). .. Purified BAC DNA was amplified using Whole genome amplification kit (Sigma-Aldrich WGA1), 20 µg of amplified DNA was then digested using a cocktail of AluI, Rsa, MseI, MspI, HaeIII, and BfuCl (New England BioLabs, Ipswich, MA, USA) overnight at 37 °C, ethanol precipitated, and resuspended in 36 µL ddH2 O. DNA was denatured at 100 °C for 1 min, and then 3'-end-labeled with unmodified aminoallyl dUTP and terminal deoxynucleotidyl transferase (Roche, Mannheim, Germany).

    Transformation Assay:

    Article Title: High Heterogeneity of Escherichia coli Sequence Types Harbouring ESBL/AmpC Genes on IncI1 Plasmids in the Colombian Poultry Chain
    Article Snippet: Plasmids were purified from the original donor strain using the Qiagen Plasmid Midi-kit (Qiagen, Germany). .. Plasmid DNA was transformed into ElectroMAX DH10B cells through electroporation (Invitrogen, USA) by mixing 5μl of isolated plasmid DNA with 20μl of electro-competent cells.

    Article Title: Three-dimensional modeling of and ligand docking to vitamin D receptor ligand binding domain
    Article Snippet: Escherichia coli DH5α competent cells were transformed with the vectors incorporating the desired mutations. .. The cDNAs of the clones were purified with a Qiagen Plasmid Midi-Kit (Qiagen, Chatsworth, CA).

    Article Title: Effects of optional structural elements, including two alternative amino termini and a new splicing cassette IV, on the function of the sodium–bicarbonate cotransporter NBCn1 (SLC4A7)
    Article Snippet: The resulting construct was transformed into ABLE C Competent Cells (Cat. No. 200171, Agilent Technologies, Santa Clara, CA, USA). .. In total, 250 ml of bacterial culture was grown from one single colony and plasmids containing the cDNA of human NBCn1 were isolated with a QIAGEN Plasmid Midi Kit (Cat. No. 12143, QIAGEN, Germantown, MD, USA) according to the manufacturer's instructions.

    Hybridization:

    Article Title: Survivin Expression and Prognostic Significance in Pediatric Malignant Peripheral Nerve Sheath Tumors (MPNST)
    Article Snippet: The BAC probe was prepared from bacterial cultures using Qiagen-Plasmid Midi kit (Qiagen GmnH, Dϋsseldorf, Germany) and labelled by nick translation with SpectrumOrange-dUTP (Visys). .. Before being used for the analysis of tumor samples, the probe was assessed by PCR with primers specific for survivin gene (primer pair For 5′- GTG AAC GGA TAC CTC TCT ATA TGC TG-3′ and Rev 5′-CTG ACT ATC ACC GTT ACC AGA ACT G-3′ and the following conditions: denaturation 1 min at 94°C, annealing 2 min at 59°C and extension 3 min at 72°C for 35 cycles; the expected length of the amplified DNA was 949 bp), and by FISH on normal metaphases from PHA-stimulated peripheral blood mononuclear cells to check adequacy and consistency of hybridization signal.

    Article Title: Assignment of Chinook Salmon (Oncorhynchus tshawytscha) Linkage Groups to Specific Chromosomes Reveals a Karyotype with Multiple Rearrangements of the Chromosome Arms of Rainbow Trout (Oncorhynchus mykiss)
    Article Snippet: BAC DNA was isolated from clones containing a microsatellite marker with a known position in the rainbow trout linkage map using the QIAGEN Plasmid Midi Kit (catalog #12143) following the manufacturer’s protocol. .. Hybridization with fluorochrome-labeled dUTPs was as suggested by the manufacturer (Vysis) with minor modifications ( ).

    Electroporation:

    Article Title: High Heterogeneity of Escherichia coli Sequence Types Harbouring ESBL/AmpC Genes on IncI1 Plasmids in the Colombian Poultry Chain
    Article Snippet: Plasmids were purified from the original donor strain using the Qiagen Plasmid Midi-kit (Qiagen, Germany). .. Plasmid DNA was transformed into ElectroMAX DH10B cells through electroporation (Invitrogen, USA) by mixing 5μl of isolated plasmid DNA with 20μl of electro-competent cells.

    Flow Cytometry:

    Article Title: Haplotype Analysis of the Pre-harvest Sprouting Resistance Locus Phs-A1 Reveals a Causal Role of TaMKK3-A in Global Germplasm
    Article Snippet: Physical Map Sequence Assembly and Annotation A fingerprinted Bacterial Artificial Chromosome (BAC) library of flow-sorted 4A chromosome was used for constructing the Chinese Spring Phs-A1 physical map . .. DNA of the BACs was extracted using the Qiagen Plasmid Midi Kit (Qiagen, Cat. No. 12143).

    Nick Translation:

    Article Title: Survivin Expression and Prognostic Significance in Pediatric Malignant Peripheral Nerve Sheath Tumors (MPNST)
    Article Snippet: .. The BAC probe was prepared from bacterial cultures using Qiagen-Plasmid Midi kit (Qiagen GmnH, Dϋsseldorf, Germany) and labelled by nick translation with SpectrumOrange-dUTP (Visys). .. Before being used for the analysis of tumor samples, the probe was assessed by PCR with primers specific for survivin gene (primer pair For 5′- GTG AAC GGA TAC CTC TCT ATA TGC TG-3′ and Rev 5′-CTG ACT ATC ACC GTT ACC AGA ACT G-3′ and the following conditions: denaturation 1 min at 94°C, annealing 2 min at 59°C and extension 3 min at 72°C for 35 cycles; the expected length of the amplified DNA was 949 bp), and by FISH on normal metaphases from PHA-stimulated peripheral blood mononuclear cells to check adequacy and consistency of hybridization signal.

    Cell Culture:

    Article Title: Mrg15 stimulates Ash1 H3K36 methyltransferase activity and facilitates Ash1 Trithorax group protein function in Drosophila
    Article Snippet: Fly stocks Flies were cultured on standard food medium at 25 °C. .. Ash1 R1288A gRNA and donor plasmids were purified using a Qiagen Plasmid Midi Kit (#12145) and introduced into nos-Cas9 embryos using standard procedures.

    Article Title: Condensins are Required for Maintenance of Nuclear Architecture
    Article Snippet: .. BAC clones were cultured and DNA was purified using QIAGEN Plasmid Midi Kit (Qiagen 12143, Hilden, Germany). .. Purified BAC DNA was amplified using Whole genome amplification kit (Sigma-Aldrich WGA1), 20 µg of amplified DNA was then digested using a cocktail of AluI, Rsa, MseI, MspI, HaeIII, and BfuCl (New England BioLabs, Ipswich, MA, USA) overnight at 37 °C, ethanol precipitated, and resuspended in 36 µL ddH2 O. DNA was denatured at 100 °C for 1 min, and then 3'-end-labeled with unmodified aminoallyl dUTP and terminal deoxynucleotidyl transferase (Roche, Mannheim, Germany).

    Article Title: Assignment of Chinook Salmon (Oncorhynchus tshawytscha) Linkage Groups to Specific Chromosomes Reveals a Karyotype with Multiple Rearrangements of the Chromosome Arms of Rainbow Trout (Oncorhynchus mykiss)
    Article Snippet: Briefly, the buffy coat was isolated from whole blood and placed in MEM media with pen-strep, L-glutamine, 10% fetal calf serum, and 200 ug/ml LPS and cultured for 6 days at 20°. .. BAC DNA was isolated from clones containing a microsatellite marker with a known position in the rainbow trout linkage map using the QIAGEN Plasmid Midi Kit (catalog #12143) following the manufacturer’s protocol.

    Generated:

    Article Title: Mrg15 stimulates Ash1 H3K36 methyltransferase activity and facilitates Ash1 Trithorax group protein function in Drosophila
    Article Snippet: The strains used in this study were as follows: nos-Cas9 (attP2) ; ash1 22 (BDSC #24161); arm-GAL4 (BDSC #1560); nos-phiC31; attP40 (BDSC #25709); and ash1 R1288A , which was generated by CRISPR/Cas9 using 5′-cggaggtacttttcgcaata-3′ gRNA and a 2 kb genomic DNA fragment with the R1288 site at the middle inserted into pEasyT vector as donor plasmid and R1288A mutation was generated by Quickchange. .. Ash1 R1288A gRNA and donor plasmids were purified using a Qiagen Plasmid Midi Kit (#12145) and introduced into nos-Cas9 embryos using standard procedures.

    Article Title: Paternal Origin of FGFR2 Mutations in Sporadic Cases of Crouzon Syndrome and Pfeiffer Syndrome
    Article Snippet: Plasmid DNA was isolated by use of the Qiagen Plasmid Midi-Kit. .. Clones were sequenced by the Johns Hopkins Genetic Research Core Facility, by means of both a 373A automated DNA sequencer (Applied Biosystems) and the fluorescent dideoxy terminator method (Smith et al. ), with use of these primers and other primers generated from the sequence.

    Article Title: Effects of optional structural elements, including two alternative amino termini and a new splicing cassette IV, on the function of the sodium–bicarbonate cotransporter NBCn1 (SLC4A7)
    Article Snippet: In total, 250 ml of bacterial culture was grown from one single colony and plasmids containing the cDNA of human NBCn1 were isolated with a QIAGEN Plasmid Midi Kit (Cat. No. 12143, QIAGEN, Germantown, MD, USA) according to the manufacturer's instructions. .. Briefly, an Xma I site was generated by site-directed mutagenesis right before the ‘ATG’ encoding the Met in the motif ‘MSTEN’ in construct pGH19-EGFP-hNBCe1-A.

    Article Title: Radiosensitization by PARP Inhibition in DNA Repair Proficient and Deficient Tumor Cells: Proliferative Recovery in Senescent Cells
    Article Snippet: HCT116 colon cancer cells were purchased from ATCC and HCT116 Ligase IV-deficient were generated as previously described [ ]. .. ATG5 and ATG7 silencing shCon, shATG5 and shATG7 plasmid constructs were isolated (Qiagen-plasmid midi kit) using bacterial stocks (Sigma-Aldrich).

    DNA Sequencing:

    Article Title: Ovaries absent links dLsd1 to HP1a for local H3K4 demethylation required for heterochromatic gene silencing
    Article Snippet: .. All the plasmids were purified using a Qiagen Plasmid Midi Kit (#12145) and the DNA sequencing verified plasmids were introduced into embryos using either P-element or nos-phiC31 system to generate transgenic flies according to a standard procedure. ..

    DNA Labeling:

    Article Title: Condensins are Required for Maintenance of Nuclear Architecture
    Article Snippet: BAC clones were cultured and DNA was purified using QIAGEN Plasmid Midi Kit (Qiagen 12143, Hilden, Germany). .. DNA was ethanol precipitated, resuspended in 10 μL ddH2O, and then conjugated to fluorophores using ARES Alexa Fluor DNA labeling kits (A-21665, A21667, and A-21676; Life Technologies) for 2 h, according to the manufacturer’s instructions.

    Polymerase Chain Reaction:

    Article Title: Role of the FeoB Protein and Siderophore in Promoting Virulence of Xanthomonas oryzae pv. oryzae on Rice ▿
    Article Snippet: Plasmid DNA was isolated by the alkaline lysis method ( ) or by using a Qiagen plasmid midi-kit. .. Phusion polymerase (Finnzymes) was used for PCR amplification in applications that required high-fidelity DNA synthesis (such as for complementation analysis) while Taq polymerase was used for all other applications.

    Article Title: ALTHEA Gold Libraries™: antibody libraries for therapeutic antibody discovery
    Article Snippet: Amplicon preparation and NGS data generation Plasmid DNA from PLs, FLs and SLs were purified using QIAGEN Plasmid Midi Kit (Cat No.: 12143) and used as templates to generate two amplicons of approximately 300 bps. .. The PCR reactions were performed as follows: 5 min start at 95°C followed by 10 cycles of 1 min at 95°C, 1 min at 67°C, 1 min at 72°C and terminated by a 10-min extension at 72°C.

    Article Title: Paternal Origin of FGFR2 Mutations in Sporadic Cases of Crouzon Syndrome and Pfeiffer Syndrome
    Article Snippet: The 1,663-bp PCR product was cloned by use of the TA Cloning Kit (Invitrogen). .. Plasmid DNA was isolated by use of the Qiagen Plasmid Midi-Kit.

    Article Title: Survivin Expression and Prognostic Significance in Pediatric Malignant Peripheral Nerve Sheath Tumors (MPNST)
    Article Snippet: The BAC probe was prepared from bacterial cultures using Qiagen-Plasmid Midi kit (Qiagen GmnH, Dϋsseldorf, Germany) and labelled by nick translation with SpectrumOrange-dUTP (Visys). .. Before being used for the analysis of tumor samples, the probe was assessed by PCR with primers specific for survivin gene (primer pair For 5′- GTG AAC GGA TAC CTC TCT ATA TGC TG-3′ and Rev 5′-CTG ACT ATC ACC GTT ACC AGA ACT G-3′ and the following conditions: denaturation 1 min at 94°C, annealing 2 min at 59°C and extension 3 min at 72°C for 35 cycles; the expected length of the amplified DNA was 949 bp), and by FISH on normal metaphases from PHA-stimulated peripheral blood mononuclear cells to check adequacy and consistency of hybridization signal.

    Injection:

    Article Title: Ovaries absent links dLsd1 to HP1a for local H3K4 demethylation required for heterochromatic gene silencing
    Article Snippet: For the lacO-terminator-GFP reporter, 555 bp VASA terminator was injected immediately following start codon of GFP in the lacO-GFP reporter. .. All the plasmids were purified using a Qiagen Plasmid Midi Kit (#12145) and the DNA sequencing verified plasmids were introduced into embryos using either P-element or nos-phiC31 system to generate transgenic flies according to a standard procedure.

    Pulsed-Field Gel:

    Article Title: Regional Spread of Pseudomonas aeruginosa ST357 Producing IMP-7 Metallo-?-Lactamase in Central Europe ▿
    Article Snippet: Total DNA from the isolates was purified in agarose plugs as described previously , and undigested DNA and DNA digested with S1 nuclease (Takara, Otsu, Japan) were run by pulsed-field gel electrophoresis (PFGE). .. Late-logarithmic cultures of the isolates were subjected to a plasmid purification procedure with the Qiagen plasmid midi-kit (Qiagen, Hilden, Germany).

    DNA Extraction:

    Article Title: Role of the FeoB Protein and Siderophore in Promoting Virulence of Xanthomonas oryzae pv. oryzae on Rice ▿
    Article Snippet: Genomic DNA isolation was performed as described by Leach et al. ( ). .. Plasmid DNA was isolated by the alkaline lysis method ( ) or by using a Qiagen plasmid midi-kit.

    Mutagenesis:

    Article Title: Mrg15 stimulates Ash1 H3K36 methyltransferase activity and facilitates Ash1 Trithorax group protein function in Drosophila
    Article Snippet: The strains used in this study were as follows: nos-Cas9 (attP2) ; ash1 22 (BDSC #24161); arm-GAL4 (BDSC #1560); nos-phiC31; attP40 (BDSC #25709); and ash1 R1288A , which was generated by CRISPR/Cas9 using 5′-cggaggtacttttcgcaata-3′ gRNA and a 2 kb genomic DNA fragment with the R1288 site at the middle inserted into pEasyT vector as donor plasmid and R1288A mutation was generated by Quickchange. .. Ash1 R1288A gRNA and donor plasmids were purified using a Qiagen Plasmid Midi Kit (#12145) and introduced into nos-Cas9 embryos using standard procedures.

    Article Title: Three-dimensional modeling of and ligand docking to vitamin D receptor ligand binding domain
    Article Snippet: Paragraph title: Site-Directed Mutagenesis. ... The cDNAs of the clones were purified with a Qiagen Plasmid Midi-Kit (Qiagen, Chatsworth, CA).

    Article Title: Effects of optional structural elements, including two alternative amino termini and a new splicing cassette IV, on the function of the sodium–bicarbonate cotransporter NBCn1 (SLC4A7)
    Article Snippet: In total, 250 ml of bacterial culture was grown from one single colony and plasmids containing the cDNA of human NBCn1 were isolated with a QIAGEN Plasmid Midi Kit (Cat. No. 12143, QIAGEN, Germantown, MD, USA) according to the manufacturer's instructions. .. Briefly, an Xma I site was generated by site-directed mutagenesis right before the ‘ATG’ encoding the Met in the motif ‘MSTEN’ in construct pGH19-EGFP-hNBCe1-A.

    Isolation:

    Article Title: High Heterogeneity of Escherichia coli Sequence Types Harbouring ESBL/AmpC Genes on IncI1 Plasmids in the Colombian Poultry Chain
    Article Snippet: Plasmids were purified from the original donor strain using the Qiagen Plasmid Midi-kit (Qiagen, Germany). .. Plasmid DNA was transformed into ElectroMAX DH10B cells through electroporation (Invitrogen, USA) by mixing 5μl of isolated plasmid DNA with 20μl of electro-competent cells.

    Article Title: Role of the FeoB Protein and Siderophore in Promoting Virulence of Xanthomonas oryzae pv. oryzae on Rice ▿
    Article Snippet: .. Plasmid DNA was isolated by the alkaline lysis method ( ) or by using a Qiagen plasmid midi-kit. .. Phusion polymerase (Finnzymes) was used for PCR amplification in applications that required high-fidelity DNA synthesis (such as for complementation analysis) while Taq polymerase was used for all other applications.

    Article Title: Paternal Origin of FGFR2 Mutations in Sporadic Cases of Crouzon Syndrome and Pfeiffer Syndrome
    Article Snippet: .. Plasmid DNA was isolated by use of the Qiagen Plasmid Midi-Kit. .. Clones were sequenced by the Johns Hopkins Genetic Research Core Facility, by means of both a 373A automated DNA sequencer (Applied Biosystems) and the fluorescent dideoxy terminator method (Smith et al. ), with use of these primers and other primers generated from the sequence.

    Article Title: Effects of optional structural elements, including two alternative amino termini and a new splicing cassette IV, on the function of the sodium–bicarbonate cotransporter NBCn1 (SLC4A7)
    Article Snippet: .. In total, 250 ml of bacterial culture was grown from one single colony and plasmids containing the cDNA of human NBCn1 were isolated with a QIAGEN Plasmid Midi Kit (Cat. No. 12143, QIAGEN, Germantown, MD, USA) according to the manufacturer's instructions. ..

    Article Title: Assignment of Chinook Salmon (Oncorhynchus tshawytscha) Linkage Groups to Specific Chromosomes Reveals a Karyotype with Multiple Rearrangements of the Chromosome Arms of Rainbow Trout (Oncorhynchus mykiss)
    Article Snippet: .. BAC DNA was isolated from clones containing a microsatellite marker with a known position in the rainbow trout linkage map using the QIAGEN Plasmid Midi Kit (catalog #12143) following the manufacturer’s protocol. .. BAC DNA was labeled with either spectrum orange (Vysis) or digoxigenin (Roche), as recommended by the manufacturers.

    Article Title: Radiosensitization by PARP Inhibition in DNA Repair Proficient and Deficient Tumor Cells: Proliferative Recovery in Senescent Cells
    Article Snippet: .. ATG5 and ATG7 silencing shCon, shATG5 and shATG7 plasmid constructs were isolated (Qiagen-plasmid midi kit) using bacterial stocks (Sigma-Aldrich). .. Plasmid constructs were packaged into lentiviral particles using HEK 239T cells and a packaging mixture composed of Lipofectamine (Invitrogen, 11668–019), psPAX2 and pMD2.G packaging constructs (Addgene, 12260, 12259).

    Subcloning:

    Article Title: Effects of optional structural elements, including two alternative amino termini and a new splicing cassette IV, on the function of the sodium–bicarbonate cotransporter NBCn1 (SLC4A7)
    Article Snippet: Paragraph title: Subcloning of cDNAs encoding NBCn1 variants into pGH19 ... In total, 250 ml of bacterial culture was grown from one single colony and plasmids containing the cDNA of human NBCn1 were isolated with a QIAGEN Plasmid Midi Kit (Cat. No. 12143, QIAGEN, Germantown, MD, USA) according to the manufacturer's instructions.

    Labeling:

    Article Title: Assignment of Chinook Salmon (Oncorhynchus tshawytscha) Linkage Groups to Specific Chromosomes Reveals a Karyotype with Multiple Rearrangements of the Chromosome Arms of Rainbow Trout (Oncorhynchus mykiss)
    Article Snippet: BAC DNA was isolated from clones containing a microsatellite marker with a known position in the rainbow trout linkage map using the QIAGEN Plasmid Midi Kit (catalog #12143) following the manufacturer’s protocol. .. BAC DNA was labeled with either spectrum orange (Vysis) or digoxigenin (Roche), as recommended by the manufacturers.

    Purification:

    Article Title: High Heterogeneity of Escherichia coli Sequence Types Harbouring ESBL/AmpC Genes on IncI1 Plasmids in the Colombian Poultry Chain
    Article Snippet: .. Plasmids were purified from the original donor strain using the Qiagen Plasmid Midi-kit (Qiagen, Germany). .. Plasmid DNA was transformed into ElectroMAX DH10B cells through electroporation (Invitrogen, USA) by mixing 5μl of isolated plasmid DNA with 20μl of electro-competent cells.

    Article Title: Role of the FeoB Protein and Siderophore in Promoting Virulence of Xanthomonas oryzae pv. oryzae on Rice ▿
    Article Snippet: Plasmid DNA was isolated by the alkaline lysis method ( ) or by using a Qiagen plasmid midi-kit. .. PCR products and restriction enzyme-digested DNA fragments were purified using a QIAquick PCR purification kit and a QIAquick nucleotide removal kit (Qiagen), respectively.

    Article Title: ALTHEA Gold Libraries™: antibody libraries for therapeutic antibody discovery
    Article Snippet: .. Amplicon preparation and NGS data generation Plasmid DNA from PLs, FLs and SLs were purified using QIAGEN Plasmid Midi Kit (Cat No.: 12143) and used as templates to generate two amplicons of approximately 300 bps. .. The PCR reactions were performed as follows: 5 min start at 95°C followed by 10 cycles of 1 min at 95°C, 1 min at 67°C, 1 min at 72°C and terminated by a 10-min extension at 72°C.

    Article Title: Mrg15 stimulates Ash1 H3K36 methyltransferase activity and facilitates Ash1 Trithorax group protein function in Drosophila
    Article Snippet: .. Ash1 R1288A gRNA and donor plasmids were purified using a Qiagen Plasmid Midi Kit (#12145) and introduced into nos-Cas9 embryos using standard procedures. .. To make the UAST-ash1 , UAST-mrg15 , UAST-nurf55 and UAST-mrg15:nurf55 constructs, fragments were subcloned into an attB-UAST vector using ClonExpress II One Step Cloning Kit (Vazyme, C112).

    Article Title: Purification and Characterization of a Second Immunoreactive Mannoprotein from Cryptococcus neoformans That Stimulates T-Cell Responses
    Article Snippet: .. Phagemid DNA was purified by using the Qiagen plasmid Midi-kit (Qiagen, Valencia, Calif.). .. C. neoformans cDNA library was mixed with XL-Blue MRF′ Escherichia coli host cells and plated at 2 × 104 to 5 × 104 phage/plate.

    Article Title: Three-dimensional modeling of and ligand docking to vitamin D receptor ligand binding domain
    Article Snippet: .. The cDNAs of the clones were purified with a Qiagen Plasmid Midi-Kit (Qiagen, Chatsworth, CA). .. Each mutant cDNA of clones was sequenced completely to ensure that no other base changes were produced (by cycle sequencing).

    Article Title: Regional Spread of Pseudomonas aeruginosa ST357 Producing IMP-7 Metallo-?-Lactamase in Central Europe ▿
    Article Snippet: .. Late-logarithmic cultures of the isolates were subjected to a plasmid purification procedure with the Qiagen plasmid midi-kit (Qiagen, Hilden, Germany). .. The preparations obtained were treated with EcoRI (Fermentas, Vilnius, Lithuania) and electrophoresed along with the isolates' total DNAs cut with the same restriction enzyme.

    Article Title: Condensins are Required for Maintenance of Nuclear Architecture
    Article Snippet: .. BAC clones were cultured and DNA was purified using QIAGEN Plasmid Midi Kit (Qiagen 12143, Hilden, Germany). .. Purified BAC DNA was amplified using Whole genome amplification kit (Sigma-Aldrich WGA1), 20 µg of amplified DNA was then digested using a cocktail of AluI, Rsa, MseI, MspI, HaeIII, and BfuCl (New England BioLabs, Ipswich, MA, USA) overnight at 37 °C, ethanol precipitated, and resuspended in 36 µL ddH2 O. DNA was denatured at 100 °C for 1 min, and then 3'-end-labeled with unmodified aminoallyl dUTP and terminal deoxynucleotidyl transferase (Roche, Mannheim, Germany).

    Article Title: Ovaries absent links dLsd1 to HP1a for local H3K4 demethylation required for heterochromatic gene silencing
    Article Snippet: .. All the plasmids were purified using a Qiagen Plasmid Midi Kit (#12145) and the DNA sequencing verified plasmids were introduced into embryos using either P-element or nos-phiC31 system to generate transgenic flies according to a standard procedure. ..

    Sequencing:

    Article Title: Three-dimensional modeling of and ligand docking to vitamin D receptor ligand binding domain
    Article Snippet: The cDNAs of the clones were purified with a Qiagen Plasmid Midi-Kit (Qiagen, Chatsworth, CA). .. Each mutant cDNA of clones was sequenced completely to ensure that no other base changes were produced (by cycle sequencing).

    Article Title: Paternal Origin of FGFR2 Mutations in Sporadic Cases of Crouzon Syndrome and Pfeiffer Syndrome
    Article Snippet: Plasmid DNA was isolated by use of the Qiagen Plasmid Midi-Kit. .. Clones were sequenced by the Johns Hopkins Genetic Research Core Facility, by means of both a 373A automated DNA sequencer (Applied Biosystems) and the fluorescent dideoxy terminator method (Smith et al. ), with use of these primers and other primers generated from the sequence.

    Article Title: Haplotype Analysis of the Pre-harvest Sprouting Resistance Locus Phs-A1 Reveals a Causal Role of TaMKK3-A in Global Germplasm
    Article Snippet: Paragraph title: Physical Map Sequence Assembly and Annotation ... DNA of the BACs was extracted using the Qiagen Plasmid Midi Kit (Qiagen, Cat. No. 12143).

    CRISPR:

    Article Title: Mrg15 stimulates Ash1 H3K36 methyltransferase activity and facilitates Ash1 Trithorax group protein function in Drosophila
    Article Snippet: The strains used in this study were as follows: nos-Cas9 (attP2) ; ash1 22 (BDSC #24161); arm-GAL4 (BDSC #1560); nos-phiC31; attP40 (BDSC #25709); and ash1 R1288A , which was generated by CRISPR/Cas9 using 5′-cggaggtacttttcgcaata-3′ gRNA and a 2 kb genomic DNA fragment with the R1288 site at the middle inserted into pEasyT vector as donor plasmid and R1288A mutation was generated by Quickchange. .. Ash1 R1288A gRNA and donor plasmids were purified using a Qiagen Plasmid Midi Kit (#12145) and introduced into nos-Cas9 embryos using standard procedures.

    cDNA Library Assay:

    Article Title: Purification and Characterization of a Second Immunoreactive Mannoprotein from Cryptococcus neoformans That Stimulates T-Cell Responses
    Article Snippet: A cDNA library of C. neoformans B3501 constructed in the Uni-Zap XR vector (Stratagene) has been previously described ( ). .. Phagemid DNA was purified by using the Qiagen plasmid Midi-kit (Qiagen, Valencia, Calif.).

    Activated Clotting Time Assay:

    Article Title: Survivin Expression and Prognostic Significance in Pediatric Malignant Peripheral Nerve Sheath Tumors (MPNST)
    Article Snippet: The BAC probe was prepared from bacterial cultures using Qiagen-Plasmid Midi kit (Qiagen GmnH, Dϋsseldorf, Germany) and labelled by nick translation with SpectrumOrange-dUTP (Visys). .. Before being used for the analysis of tumor samples, the probe was assessed by PCR with primers specific for survivin gene (primer pair For 5′- GTG AAC GGA TAC CTC TCT ATA TGC TG-3′ and Rev 5′-CTG ACT ATC ACC GTT ACC AGA ACT G-3′ and the following conditions: denaturation 1 min at 94°C, annealing 2 min at 59°C and extension 3 min at 72°C for 35 cycles; the expected length of the amplified DNA was 949 bp), and by FISH on normal metaphases from PHA-stimulated peripheral blood mononuclear cells to check adequacy and consistency of hybridization signal.

    In Situ Hybridization:

    Article Title: Assignment of Chinook Salmon (Oncorhynchus tshawytscha) Linkage Groups to Specific Chromosomes Reveals a Karyotype with Multiple Rearrangements of the Chromosome Arms of Rainbow Trout (Oncorhynchus mykiss)
    Article Snippet: Paragraph title: In situ hybridization and karyotyping ... BAC DNA was isolated from clones containing a microsatellite marker with a known position in the rainbow trout linkage map using the QIAGEN Plasmid Midi Kit (catalog #12143) following the manufacturer’s protocol.

    Plasmid Preparation:

    Article Title: High Heterogeneity of Escherichia coli Sequence Types Harbouring ESBL/AmpC Genes on IncI1 Plasmids in the Colombian Poultry Chain
    Article Snippet: .. Plasmids were purified from the original donor strain using the Qiagen Plasmid Midi-kit (Qiagen, Germany). .. Plasmid DNA was transformed into ElectroMAX DH10B cells through electroporation (Invitrogen, USA) by mixing 5μl of isolated plasmid DNA with 20μl of electro-competent cells.

    Article Title: Role of the FeoB Protein and Siderophore in Promoting Virulence of Xanthomonas oryzae pv. oryzae on Rice ▿
    Article Snippet: .. Plasmid DNA was isolated by the alkaline lysis method ( ) or by using a Qiagen plasmid midi-kit. .. Phusion polymerase (Finnzymes) was used for PCR amplification in applications that required high-fidelity DNA synthesis (such as for complementation analysis) while Taq polymerase was used for all other applications.

    Article Title: ALTHEA Gold Libraries™: antibody libraries for therapeutic antibody discovery
    Article Snippet: .. Amplicon preparation and NGS data generation Plasmid DNA from PLs, FLs and SLs were purified using QIAGEN Plasmid Midi Kit (Cat No.: 12143) and used as templates to generate two amplicons of approximately 300 bps. .. The PCR reactions were performed as follows: 5 min start at 95°C followed by 10 cycles of 1 min at 95°C, 1 min at 67°C, 1 min at 72°C and terminated by a 10-min extension at 72°C.

    Article Title: Mrg15 stimulates Ash1 H3K36 methyltransferase activity and facilitates Ash1 Trithorax group protein function in Drosophila
    Article Snippet: .. Ash1 R1288A gRNA and donor plasmids were purified using a Qiagen Plasmid Midi Kit (#12145) and introduced into nos-Cas9 embryos using standard procedures. .. To make the UAST-ash1 , UAST-mrg15 , UAST-nurf55 and UAST-mrg15:nurf55 constructs, fragments were subcloned into an attB-UAST vector using ClonExpress II One Step Cloning Kit (Vazyme, C112).

    Article Title: Purification and Characterization of a Second Immunoreactive Mannoprotein from Cryptococcus neoformans That Stimulates T-Cell Responses
    Article Snippet: .. Phagemid DNA was purified by using the Qiagen plasmid Midi-kit (Qiagen, Valencia, Calif.). .. C. neoformans cDNA library was mixed with XL-Blue MRF′ Escherichia coli host cells and plated at 2 × 104 to 5 × 104 phage/plate.

    Article Title: Three-dimensional modeling of and ligand docking to vitamin D receptor ligand binding domain
    Article Snippet: .. The cDNAs of the clones were purified with a Qiagen Plasmid Midi-Kit (Qiagen, Chatsworth, CA). .. Each mutant cDNA of clones was sequenced completely to ensure that no other base changes were produced (by cycle sequencing).

    Article Title: Paternal Origin of FGFR2 Mutations in Sporadic Cases of Crouzon Syndrome and Pfeiffer Syndrome
    Article Snippet: .. Plasmid DNA was isolated by use of the Qiagen Plasmid Midi-Kit. .. Clones were sequenced by the Johns Hopkins Genetic Research Core Facility, by means of both a 373A automated DNA sequencer (Applied Biosystems) and the fluorescent dideoxy terminator method (Smith et al. ), with use of these primers and other primers generated from the sequence.

    Article Title: Effects of optional structural elements, including two alternative amino termini and a new splicing cassette IV, on the function of the sodium–bicarbonate cotransporter NBCn1 (SLC4A7)
    Article Snippet: .. In total, 250 ml of bacterial culture was grown from one single colony and plasmids containing the cDNA of human NBCn1 were isolated with a QIAGEN Plasmid Midi Kit (Cat. No. 12143, QIAGEN, Germantown, MD, USA) according to the manufacturer's instructions. ..

    Article Title: Haplotype Analysis of the Pre-harvest Sprouting Resistance Locus Phs-A1 Reveals a Causal Role of TaMKK3-A in Global Germplasm
    Article Snippet: .. DNA of the BACs was extracted using the Qiagen Plasmid Midi Kit (Qiagen, Cat. No. 12143). .. Eleven of the 20 MTP BACs containing and distal to PM19-A1 in the physical map of Cluster 16421 and the four MTP BACs of Cluster 285 were sequenced on the Illumina MiSeq with 250 bp paired-end reads.

    Article Title: Regional Spread of Pseudomonas aeruginosa ST357 Producing IMP-7 Metallo-?-Lactamase in Central Europe ▿
    Article Snippet: .. Late-logarithmic cultures of the isolates were subjected to a plasmid purification procedure with the Qiagen plasmid midi-kit (Qiagen, Hilden, Germany). .. The preparations obtained were treated with EcoRI (Fermentas, Vilnius, Lithuania) and electrophoresed along with the isolates' total DNAs cut with the same restriction enzyme.

    Article Title: Condensins are Required for Maintenance of Nuclear Architecture
    Article Snippet: .. BAC clones were cultured and DNA was purified using QIAGEN Plasmid Midi Kit (Qiagen 12143, Hilden, Germany). .. Purified BAC DNA was amplified using Whole genome amplification kit (Sigma-Aldrich WGA1), 20 µg of amplified DNA was then digested using a cocktail of AluI, Rsa, MseI, MspI, HaeIII, and BfuCl (New England BioLabs, Ipswich, MA, USA) overnight at 37 °C, ethanol precipitated, and resuspended in 36 µL ddH2 O. DNA was denatured at 100 °C for 1 min, and then 3'-end-labeled with unmodified aminoallyl dUTP and terminal deoxynucleotidyl transferase (Roche, Mannheim, Germany).

    Article Title: Assignment of Chinook Salmon (Oncorhynchus tshawytscha) Linkage Groups to Specific Chromosomes Reveals a Karyotype with Multiple Rearrangements of the Chromosome Arms of Rainbow Trout (Oncorhynchus mykiss)
    Article Snippet: .. BAC DNA was isolated from clones containing a microsatellite marker with a known position in the rainbow trout linkage map using the QIAGEN Plasmid Midi Kit (catalog #12143) following the manufacturer’s protocol. .. BAC DNA was labeled with either spectrum orange (Vysis) or digoxigenin (Roche), as recommended by the manufacturers.

    Article Title: Ovaries absent links dLsd1 to HP1a for local H3K4 demethylation required for heterochromatic gene silencing
    Article Snippet: .. All the plasmids were purified using a Qiagen Plasmid Midi Kit (#12145) and the DNA sequencing verified plasmids were introduced into embryos using either P-element or nos-phiC31 system to generate transgenic flies according to a standard procedure. ..

    Selection:

    Article Title: High Heterogeneity of Escherichia coli Sequence Types Harbouring ESBL/AmpC Genes on IncI1 Plasmids in the Colombian Poultry Chain
    Article Snippet: For the highly prevalent bla CMY and bla SHV genes, a random selection was made using the Random function in Microsoft® Excel to assign random numbers to isolates positive for bla CMY and bla SHV . .. Plasmids were purified from the original donor strain using the Qiagen Plasmid Midi-kit (Qiagen, Germany).

    Article Title: Radiosensitization by PARP Inhibition in DNA Repair Proficient and Deficient Tumor Cells: Proliferative Recovery in Senescent Cells
    Article Snippet: ATG5 and ATG7 silencing shCon, shATG5 and shATG7 plasmid constructs were isolated (Qiagen-plasmid midi kit) using bacterial stocks (Sigma-Aldrich). .. Infected cells were then maintained with the selection marker, puromycin (2 µg/mL) throughout the course of the study.

    Transgenic Assay:

    Article Title: Mrg15 stimulates Ash1 H3K36 methyltransferase activity and facilitates Ash1 Trithorax group protein function in Drosophila
    Article Snippet: Ash1 R1288A gRNA and donor plasmids were purified using a Qiagen Plasmid Midi Kit (#12145) and introduced into nos-Cas9 embryos using standard procedures. .. The plasmid DNA was introduced to nos-phiC31;attP40 embryos using a standard procedure to generate transgenic flies.

    Article Title: Ovaries absent links dLsd1 to HP1a for local H3K4 demethylation required for heterochromatic gene silencing
    Article Snippet: .. All the plasmids were purified using a Qiagen Plasmid Midi Kit (#12145) and the DNA sequencing verified plasmids were introduced into embryos using either P-element or nos-phiC31 system to generate transgenic flies according to a standard procedure. ..

    Next-Generation Sequencing:

    Article Title: ALTHEA Gold Libraries™: antibody libraries for therapeutic antibody discovery
    Article Snippet: .. Amplicon preparation and NGS data generation Plasmid DNA from PLs, FLs and SLs were purified using QIAGEN Plasmid Midi Kit (Cat No.: 12143) and used as templates to generate two amplicons of approximately 300 bps. .. The PCR reactions were performed as follows: 5 min start at 95°C followed by 10 cycles of 1 min at 95°C, 1 min at 67°C, 1 min at 72°C and terminated by a 10-min extension at 72°C.

    Knock-Out:

    Article Title: Ovaries absent links dLsd1 to HP1a for local H3K4 demethylation required for heterochromatic gene silencing
    Article Snippet: Paragraph title: Generation of knock-out and transgenic flies ... All the plasmids were purified using a Qiagen Plasmid Midi Kit (#12145) and the DNA sequencing verified plasmids were introduced into embryos using either P-element or nos-phiC31 system to generate transgenic flies according to a standard procedure.

    Produced:

    Article Title: Three-dimensional modeling of and ligand docking to vitamin D receptor ligand binding domain
    Article Snippet: Five clones of mutated hVDRs (S237A, S275A, S278A, C288A, and H397A) were produced by changing the corresponding amino acid residue into alanine according to the manufacturer's instructions. .. The cDNAs of the clones were purified with a Qiagen Plasmid Midi-Kit (Qiagen, Chatsworth, CA).

    Article Title: Haplotype Analysis of the Pre-harvest Sprouting Resistance Locus Phs-A1 Reveals a Causal Role of TaMKK3-A in Global Germplasm
    Article Snippet: DNA of the BACs was extracted using the Qiagen Plasmid Midi Kit (Qiagen, Cat. No. 12143). .. An average of 2,105,488 and 2,752,220 paired-end reads per BAC were produced for Cluster 16421 and 285 BACs, respectively.

    Alkaline Lysis:

    Article Title: Role of the FeoB Protein and Siderophore in Promoting Virulence of Xanthomonas oryzae pv. oryzae on Rice ▿
    Article Snippet: .. Plasmid DNA was isolated by the alkaline lysis method ( ) or by using a Qiagen plasmid midi-kit. .. Phusion polymerase (Finnzymes) was used for PCR amplification in applications that required high-fidelity DNA synthesis (such as for complementation analysis) while Taq polymerase was used for all other applications.

    BAC Assay:

    Article Title: Haplotype Analysis of the Pre-harvest Sprouting Resistance Locus Phs-A1 Reveals a Causal Role of TaMKK3-A in Global Germplasm
    Article Snippet: The PM19-A1 -containing BACs belong to BAC Cluster 16421 which has 20 BACs in its MTP while the TaMKK3-A -containing BACs belong to BAC Cluster 285 comprised of four MTP BACs (Supplementary Table ). .. DNA of the BACs was extracted using the Qiagen Plasmid Midi Kit (Qiagen, Cat. No. 12143).

    Article Title: Survivin Expression and Prognostic Significance in Pediatric Malignant Peripheral Nerve Sheath Tumors (MPNST)
    Article Snippet: .. The BAC probe was prepared from bacterial cultures using Qiagen-Plasmid Midi kit (Qiagen GmnH, Dϋsseldorf, Germany) and labelled by nick translation with SpectrumOrange-dUTP (Visys). .. Before being used for the analysis of tumor samples, the probe was assessed by PCR with primers specific for survivin gene (primer pair For 5′- GTG AAC GGA TAC CTC TCT ATA TGC TG-3′ and Rev 5′-CTG ACT ATC ACC GTT ACC AGA ACT G-3′ and the following conditions: denaturation 1 min at 94°C, annealing 2 min at 59°C and extension 3 min at 72°C for 35 cycles; the expected length of the amplified DNA was 949 bp), and by FISH on normal metaphases from PHA-stimulated peripheral blood mononuclear cells to check adequacy and consistency of hybridization signal.

    Article Title: Condensins are Required for Maintenance of Nuclear Architecture
    Article Snippet: .. BAC clones were cultured and DNA was purified using QIAGEN Plasmid Midi Kit (Qiagen 12143, Hilden, Germany). .. Purified BAC DNA was amplified using Whole genome amplification kit (Sigma-Aldrich WGA1), 20 µg of amplified DNA was then digested using a cocktail of AluI, Rsa, MseI, MspI, HaeIII, and BfuCl (New England BioLabs, Ipswich, MA, USA) overnight at 37 °C, ethanol precipitated, and resuspended in 36 µL ddH2 O. DNA was denatured at 100 °C for 1 min, and then 3'-end-labeled with unmodified aminoallyl dUTP and terminal deoxynucleotidyl transferase (Roche, Mannheim, Germany).

    Article Title: Assignment of Chinook Salmon (Oncorhynchus tshawytscha) Linkage Groups to Specific Chromosomes Reveals a Karyotype with Multiple Rearrangements of the Chromosome Arms of Rainbow Trout (Oncorhynchus mykiss)
    Article Snippet: .. BAC DNA was isolated from clones containing a microsatellite marker with a known position in the rainbow trout linkage map using the QIAGEN Plasmid Midi Kit (catalog #12143) following the manufacturer’s protocol. .. BAC DNA was labeled with either spectrum orange (Vysis) or digoxigenin (Roche), as recommended by the manufacturers.

    Marker:

    Article Title: Assignment of Chinook Salmon (Oncorhynchus tshawytscha) Linkage Groups to Specific Chromosomes Reveals a Karyotype with Multiple Rearrangements of the Chromosome Arms of Rainbow Trout (Oncorhynchus mykiss)
    Article Snippet: .. BAC DNA was isolated from clones containing a microsatellite marker with a known position in the rainbow trout linkage map using the QIAGEN Plasmid Midi Kit (catalog #12143) following the manufacturer’s protocol. .. BAC DNA was labeled with either spectrum orange (Vysis) or digoxigenin (Roche), as recommended by the manufacturers.

    Article Title: Radiosensitization by PARP Inhibition in DNA Repair Proficient and Deficient Tumor Cells: Proliferative Recovery in Senescent Cells
    Article Snippet: ATG5 and ATG7 silencing shCon, shATG5 and shATG7 plasmid constructs were isolated (Qiagen-plasmid midi kit) using bacterial stocks (Sigma-Aldrich). .. Infected cells were then maintained with the selection marker, puromycin (2 µg/mL) throughout the course of the study.

    Fluorescence In Situ Hybridization:

    Article Title: Survivin Expression and Prognostic Significance in Pediatric Malignant Peripheral Nerve Sheath Tumors (MPNST)
    Article Snippet: Paragraph title: FISH analysis ... The BAC probe was prepared from bacterial cultures using Qiagen-Plasmid Midi kit (Qiagen GmnH, Dϋsseldorf, Germany) and labelled by nick translation with SpectrumOrange-dUTP (Visys).

    Article Title: Condensins are Required for Maintenance of Nuclear Architecture
    Article Snippet: Paragraph title: 2.4. FISH Probe Preparation ... BAC clones were cultured and DNA was purified using QIAGEN Plasmid Midi Kit (Qiagen 12143, Hilden, Germany).

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