anti m13 mouse monoclonal antibody  (Sino Biological)


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    Name:
    Anti M13 Antibody Mouse Monoclonal
    Description:
    This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with M13 Bacteriophage The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography
    Catalog Number:
    11973-MM05T
    Price:
    None
    Category:
    Primary Antibody
    Reactivity:
    other
    Applications:
    ELISA
    Immunogen:
    M13 Bacteriophage
    Antibody Type:
    MAb
    Host:
    Mouse
    Isotype:
    Mouse IgG1
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    Structured Review

    Sino Biological anti m13 mouse monoclonal antibody
    Detection of membrane-localized EGFP via anti-EGFP nanobody-displaying phages (A),(B) Membrane expressed EGFP. (A) Microscopy images of HEK293T cells expressing indicated constructs, showing differential localization of untagged cytosolic EGFP (pCAG-EGFP, middle) and GPI-anchored membrane-localized EGFP (pCAG-EGFP-GPI, right, Methods ). (B) Schematic of surface-exposed GPI-anchored EGFP. (C) Schematic for detection of phage recognition via flow cytometry. Phage-stained cells are incubated with mouse <t>anti-M13</t> coat protein antibodies followed by detection by Alexa Fluor 647-conjugated anti-mouse secondary antibodies. Phage binding is thus reflected by Alexa Fluor 647 signal. (D) Flow cytometry analysis of anti-EGFP phage nanobody binding to EGFP-expressing HEK293T cells. EGFP fluorescence (y axis) and phage binding (x axis, Alexa Fluor 647) in each of the HEK293T cell populations as in A , either unstained (left) or stained with an anti-EGFP phage (right). EGFP-expressing cells were always characterized by the presence of both EGFP hi and EGFP lo populations. (E) Specificity of detection. As in D but using the indicated staining controls for specific staining of membrane-EGFP-expressing cells. (F),(G) PAC-tag does not impact nanobody display and antigen interaction. EGFP fluorescence (F, y axis) and phage binding (F, x axis, Alexa Fluor 647) and distribution of level of phage binding (G) for phage-stained EGFP-GPI expressing cells using indicated phage nanobodies (for RD1 sequences see Supp. Fig. 2B ).
    This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with M13 Bacteriophage The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography
    https://www.bioz.com/result/anti m13 mouse monoclonal antibody/product/Sino Biological
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti m13 mouse monoclonal antibody - by Bioz Stars, 2021-05
    95/100 stars

    Images

    1) Product Images from "Single-cell multimodal profiling of proteins and chromatin accessibility using PHAGE-ATAC"

    Article Title: Single-cell multimodal profiling of proteins and chromatin accessibility using PHAGE-ATAC

    Journal: bioRxiv

    doi: 10.1101/2020.10.01.322420

    Detection of membrane-localized EGFP via anti-EGFP nanobody-displaying phages (A),(B) Membrane expressed EGFP. (A) Microscopy images of HEK293T cells expressing indicated constructs, showing differential localization of untagged cytosolic EGFP (pCAG-EGFP, middle) and GPI-anchored membrane-localized EGFP (pCAG-EGFP-GPI, right, Methods ). (B) Schematic of surface-exposed GPI-anchored EGFP. (C) Schematic for detection of phage recognition via flow cytometry. Phage-stained cells are incubated with mouse anti-M13 coat protein antibodies followed by detection by Alexa Fluor 647-conjugated anti-mouse secondary antibodies. Phage binding is thus reflected by Alexa Fluor 647 signal. (D) Flow cytometry analysis of anti-EGFP phage nanobody binding to EGFP-expressing HEK293T cells. EGFP fluorescence (y axis) and phage binding (x axis, Alexa Fluor 647) in each of the HEK293T cell populations as in A , either unstained (left) or stained with an anti-EGFP phage (right). EGFP-expressing cells were always characterized by the presence of both EGFP hi and EGFP lo populations. (E) Specificity of detection. As in D but using the indicated staining controls for specific staining of membrane-EGFP-expressing cells. (F),(G) PAC-tag does not impact nanobody display and antigen interaction. EGFP fluorescence (F, y axis) and phage binding (F, x axis, Alexa Fluor 647) and distribution of level of phage binding (G) for phage-stained EGFP-GPI expressing cells using indicated phage nanobodies (for RD1 sequences see Supp. Fig. 2B ).
    Figure Legend Snippet: Detection of membrane-localized EGFP via anti-EGFP nanobody-displaying phages (A),(B) Membrane expressed EGFP. (A) Microscopy images of HEK293T cells expressing indicated constructs, showing differential localization of untagged cytosolic EGFP (pCAG-EGFP, middle) and GPI-anchored membrane-localized EGFP (pCAG-EGFP-GPI, right, Methods ). (B) Schematic of surface-exposed GPI-anchored EGFP. (C) Schematic for detection of phage recognition via flow cytometry. Phage-stained cells are incubated with mouse anti-M13 coat protein antibodies followed by detection by Alexa Fluor 647-conjugated anti-mouse secondary antibodies. Phage binding is thus reflected by Alexa Fluor 647 signal. (D) Flow cytometry analysis of anti-EGFP phage nanobody binding to EGFP-expressing HEK293T cells. EGFP fluorescence (y axis) and phage binding (x axis, Alexa Fluor 647) in each of the HEK293T cell populations as in A , either unstained (left) or stained with an anti-EGFP phage (right). EGFP-expressing cells were always characterized by the presence of both EGFP hi and EGFP lo populations. (E) Specificity of detection. As in D but using the indicated staining controls for specific staining of membrane-EGFP-expressing cells. (F),(G) PAC-tag does not impact nanobody display and antigen interaction. EGFP fluorescence (F, y axis) and phage binding (F, x axis, Alexa Fluor 647) and distribution of level of phage binding (G) for phage-stained EGFP-GPI expressing cells using indicated phage nanobodies (for RD1 sequences see Supp. Fig. 2B ).

    Techniques Used: Microscopy, Expressing, Construct, Flow Cytometry, Staining, Incubation, Binding Assay, Fluorescence

    Related Articles

    Incubation:

    Article Title: Potent SARS-CoV-2 neutralizing antibodies selected from a human antibody library constructed decades ago
    Article Snippet: After removal of the incubation solution, the resulting plates were rinsed once using the PBST buffer and incubated with a blocking solution containing 5% milk (v/v) in PBST (150 μL/well) at 37 °C for 1 h. After blocking and PBST washing (once), 50 μL of phagemid-containing XL1-Blue culture medium supernatants (by centrifuging the third round panning output XL1-Blue cells at 3000 g, 15 min) mixed with 10 μL 5% milk (v/v) in PBST was added to each well and incubated at 37 °C for 1 h. The resulting plates were rinsed eight times using PBST before subjecting to horseradish peroxidase (HRP) detection. .. A solution containing the secondary antibody, anti-M13 bacteriophage antibody conjugated with HRP (dilution factor 1 : 5000; Sino Biological, #11973-MM05T-H), was added into the above plates (150 μL/well) and incubated at 37 °C for 1 h. Plates were then washed eight times with PBST followed by the addition of 50 μL ABTS solution (Roche, #11684302001) into each well. .. After ~10 min incubation at room temperature, the absorbance change at 405 nm in each well was measured on a microplate reader (Enspire, PerkinElmer).

    Article Title: A potent bispecific nanobody protects hACE2 mice against SARS-CoV-2 infection via intranasal administration
    Article Snippet: Phage ELISA 200 ng S or RBD protein in coating buffer (pH 9.6) was used to coat 96-well plates (cat.# 9018, Corning) at 4 °C overnight. .. After washing, the plates were blocked with blocking buffer (3% BSA in PBST) for 1 h at 37 °C, and then incubated with library phages or single clone phage in bacterial supernatant at 4 °C for 1.5 h. After washing, an anti-M13 bacteriophage antibody with HRP (1:10000 dilution, cat.# 11973-MM05T-H, Sino Biological) was added and incubated at 37 °C for 1 h. Accordingly, TMB substrate(Sigma) was added at 37 °C for 10 min; 10 μl 0.2 M H2 SO4 was added to stop the reaction. .. Optical densities were measured at 450 nm using the Infinite 200 (Tecan, Ramsey, MN, USA).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Therapeutic antibodies, targeting the SARS-CoV-2 spike N-terminal domain, protect lethally infected K18-hACE2 mice
    Article Snippet: ELISA Direct ELISA consisted of coating microtiter plates with 1 μg/ml of recombinant SARS-CoV-2 spike, S1 domain, RBD or NTD subunits. .. For phage ELISA, HRP-conjugated anti-M13 antibody (Sino Biological, USA, Cat# 11973-MM05T-H lot HO13AU601; used at 1:8000 working dilution) was used following detection with TMB substrate (Millipore, USA). .. ELISA of both sera and recombinant human antibodies was applied with AP-conjugated Donkey anti-human IgG (Jackson ImmunoResearch, USA, Cat# 709-055-149 lot 130049; used at 1:2000 working dilution) following detection using p -nitrophenyl phosphate (pNPP) substrate (Sigma, Israel).

    Blocking Assay:

    Article Title: A potent bispecific nanobody protects hACE2 mice against SARS-CoV-2 infection via intranasal administration
    Article Snippet: Phage ELISA 200 ng S or RBD protein in coating buffer (pH 9.6) was used to coat 96-well plates (cat.# 9018, Corning) at 4 °C overnight. .. After washing, the plates were blocked with blocking buffer (3% BSA in PBST) for 1 h at 37 °C, and then incubated with library phages or single clone phage in bacterial supernatant at 4 °C for 1.5 h. After washing, an anti-M13 bacteriophage antibody with HRP (1:10000 dilution, cat.# 11973-MM05T-H, Sino Biological) was added and incubated at 37 °C for 1 h. Accordingly, TMB substrate(Sigma) was added at 37 °C for 10 min; 10 μl 0.2 M H2 SO4 was added to stop the reaction. .. Optical densities were measured at 450 nm using the Infinite 200 (Tecan, Ramsey, MN, USA).

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  • About
  • News
  • Press Release
  • Team
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  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    Sino Biological anti m13 mouse monoclonal antibody
    Detection of membrane-localized EGFP via anti-EGFP nanobody-displaying phages (A),(B) Membrane expressed EGFP. (A) Microscopy images of HEK293T cells expressing indicated constructs, showing differential localization of untagged cytosolic EGFP (pCAG-EGFP, middle) and GPI-anchored membrane-localized EGFP (pCAG-EGFP-GPI, right, Methods ). (B) Schematic of surface-exposed GPI-anchored EGFP. (C) Schematic for detection of phage recognition via flow cytometry. Phage-stained cells are incubated with mouse <t>anti-M13</t> coat protein antibodies followed by detection by Alexa Fluor 647-conjugated anti-mouse secondary antibodies. Phage binding is thus reflected by Alexa Fluor 647 signal. (D) Flow cytometry analysis of anti-EGFP phage nanobody binding to EGFP-expressing HEK293T cells. EGFP fluorescence (y axis) and phage binding (x axis, Alexa Fluor 647) in each of the HEK293T cell populations as in A , either unstained (left) or stained with an anti-EGFP phage (right). EGFP-expressing cells were always characterized by the presence of both EGFP hi and EGFP lo populations. (E) Specificity of detection. As in D but using the indicated staining controls for specific staining of membrane-EGFP-expressing cells. (F),(G) PAC-tag does not impact nanobody display and antigen interaction. EGFP fluorescence (F, y axis) and phage binding (F, x axis, Alexa Fluor 647) and distribution of level of phage binding (G) for phage-stained EGFP-GPI expressing cells using indicated phage nanobodies (for RD1 sequences see Supp. Fig. 2B ).
    Anti M13 Mouse Monoclonal Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti m13 mouse monoclonal antibody/product/Sino Biological
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti m13 mouse monoclonal antibody - by Bioz Stars, 2021-05
    95/100 stars
      Buy from Supplier

    95
    Sino Biological anti m13 hrp antibody
    Detection of membrane-localized EGFP via anti-EGFP nanobody-displaying phages (A),(B) Membrane expressed EGFP. (A) Microscopy images of HEK293T cells expressing indicated constructs, showing differential localization of untagged cytosolic EGFP (pCAG-EGFP, middle) and GPI-anchored membrane-localized EGFP (pCAG-EGFP-GPI, right, Methods ). (B) Schematic of surface-exposed GPI-anchored EGFP. (C) Schematic for detection of phage recognition via flow cytometry. Phage-stained cells are incubated with mouse <t>anti-M13</t> coat protein antibodies followed by detection by Alexa Fluor 647-conjugated anti-mouse secondary antibodies. Phage binding is thus reflected by Alexa Fluor 647 signal. (D) Flow cytometry analysis of anti-EGFP phage nanobody binding to EGFP-expressing HEK293T cells. EGFP fluorescence (y axis) and phage binding (x axis, Alexa Fluor 647) in each of the HEK293T cell populations as in A , either unstained (left) or stained with an anti-EGFP phage (right). EGFP-expressing cells were always characterized by the presence of both EGFP hi and EGFP lo populations. (E) Specificity of detection. As in D but using the indicated staining controls for specific staining of membrane-EGFP-expressing cells. (F),(G) PAC-tag does not impact nanobody display and antigen interaction. EGFP fluorescence (F, y axis) and phage binding (F, x axis, Alexa Fluor 647) and distribution of level of phage binding (G) for phage-stained EGFP-GPI expressing cells using indicated phage nanobodies (for RD1 sequences see Supp. Fig. 2B ).
    Anti M13 Hrp Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti m13 hrp antibody/product/Sino Biological
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti m13 hrp antibody - by Bioz Stars, 2021-05
    95/100 stars
      Buy from Supplier

    Image Search Results


    Detection of membrane-localized EGFP via anti-EGFP nanobody-displaying phages (A),(B) Membrane expressed EGFP. (A) Microscopy images of HEK293T cells expressing indicated constructs, showing differential localization of untagged cytosolic EGFP (pCAG-EGFP, middle) and GPI-anchored membrane-localized EGFP (pCAG-EGFP-GPI, right, Methods ). (B) Schematic of surface-exposed GPI-anchored EGFP. (C) Schematic for detection of phage recognition via flow cytometry. Phage-stained cells are incubated with mouse anti-M13 coat protein antibodies followed by detection by Alexa Fluor 647-conjugated anti-mouse secondary antibodies. Phage binding is thus reflected by Alexa Fluor 647 signal. (D) Flow cytometry analysis of anti-EGFP phage nanobody binding to EGFP-expressing HEK293T cells. EGFP fluorescence (y axis) and phage binding (x axis, Alexa Fluor 647) in each of the HEK293T cell populations as in A , either unstained (left) or stained with an anti-EGFP phage (right). EGFP-expressing cells were always characterized by the presence of both EGFP hi and EGFP lo populations. (E) Specificity of detection. As in D but using the indicated staining controls for specific staining of membrane-EGFP-expressing cells. (F),(G) PAC-tag does not impact nanobody display and antigen interaction. EGFP fluorescence (F, y axis) and phage binding (F, x axis, Alexa Fluor 647) and distribution of level of phage binding (G) for phage-stained EGFP-GPI expressing cells using indicated phage nanobodies (for RD1 sequences see Supp. Fig. 2B ).

    Journal: bioRxiv

    Article Title: Single-cell multimodal profiling of proteins and chromatin accessibility using PHAGE-ATAC

    doi: 10.1101/2020.10.01.322420

    Figure Lengend Snippet: Detection of membrane-localized EGFP via anti-EGFP nanobody-displaying phages (A),(B) Membrane expressed EGFP. (A) Microscopy images of HEK293T cells expressing indicated constructs, showing differential localization of untagged cytosolic EGFP (pCAG-EGFP, middle) and GPI-anchored membrane-localized EGFP (pCAG-EGFP-GPI, right, Methods ). (B) Schematic of surface-exposed GPI-anchored EGFP. (C) Schematic for detection of phage recognition via flow cytometry. Phage-stained cells are incubated with mouse anti-M13 coat protein antibodies followed by detection by Alexa Fluor 647-conjugated anti-mouse secondary antibodies. Phage binding is thus reflected by Alexa Fluor 647 signal. (D) Flow cytometry analysis of anti-EGFP phage nanobody binding to EGFP-expressing HEK293T cells. EGFP fluorescence (y axis) and phage binding (x axis, Alexa Fluor 647) in each of the HEK293T cell populations as in A , either unstained (left) or stained with an anti-EGFP phage (right). EGFP-expressing cells were always characterized by the presence of both EGFP hi and EGFP lo populations. (E) Specificity of detection. As in D but using the indicated staining controls for specific staining of membrane-EGFP-expressing cells. (F),(G) PAC-tag does not impact nanobody display and antigen interaction. EGFP fluorescence (F, y axis) and phage binding (F, x axis, Alexa Fluor 647) and distribution of level of phage binding (G) for phage-stained EGFP-GPI expressing cells using indicated phage nanobodies (for RD1 sequences see Supp. Fig. 2B ).

    Article Snippet: Cells were resuspended in FC buffer and anti-M13 antibody (Sino Biological, 11973-MM05T-50) was added at 1:500 dilution.

    Techniques: Microscopy, Expressing, Construct, Flow Cytometry, Staining, Incubation, Binding Assay, Fluorescence