vre  (Sino Biological)


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    Sino Biological vre
    Hemagglutination assay using <t>VRE-treated</t> RBCs or viruses. (A) VRE-induced hemagglutination was illustrated. (B) Schematic diagram of the experimental procedures. RBCs were treated with various concentrations of VRE at RT for 30 min. The unbound VRE was removed, and the treated RBC pellet was washed and resuspended. A total of 2,000 HAU of PR8-H1N1 virus was added to the treated RBCs and incubated at RT for 30 min. Then, the RBC pellet was washed again and resuspended in PBS. (C) The amount of RBC-bound virus was determined by qRT-PCR. (D) Schematic diagram of the experimental procedures. A total of 2,000 HAU of PR8-H1N1 virus was treated with various concentrations of VRE or 2 μg of <t>polyclonal</t> antibody against the H1N1 HA protein at RT for 30 min. Then, 500 µl of 1% RBCs was added to the treated virus solution and incubated at RT for 30 min, and the RBC pellet was washed and resuspended in PBS. (E) The amount of RBC-bound virus was determined by qRT-PCR. Data are presented as the mean ± SEM of three replicates and compared by one-way ANOVA followed by Dunnett’s multiple comparisons test (ns, non-significant; * p
    Vre, supplied by Sino Biological, used in various techniques. Bioz Stars score: 91/100, based on 0 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    vre - by Bioz Stars, 2021-02
    91/100 stars

    Images

    1) Product Images from "Vigna radiata (L.) R. Wilczek Extract Inhibits Influenza A Virus by Targeting Viral Attachment, Penetration, Assembly, and Release"

    Article Title: Vigna radiata (L.) R. Wilczek Extract Inhibits Influenza A Virus by Targeting Viral Attachment, Penetration, Assembly, and Release

    Journal: Frontiers in Pharmacology

    doi: 10.3389/fphar.2020.584973

    Hemagglutination assay using VRE-treated RBCs or viruses. (A) VRE-induced hemagglutination was illustrated. (B) Schematic diagram of the experimental procedures. RBCs were treated with various concentrations of VRE at RT for 30 min. The unbound VRE was removed, and the treated RBC pellet was washed and resuspended. A total of 2,000 HAU of PR8-H1N1 virus was added to the treated RBCs and incubated at RT for 30 min. Then, the RBC pellet was washed again and resuspended in PBS. (C) The amount of RBC-bound virus was determined by qRT-PCR. (D) Schematic diagram of the experimental procedures. A total of 2,000 HAU of PR8-H1N1 virus was treated with various concentrations of VRE or 2 μg of polyclonal antibody against the H1N1 HA protein at RT for 30 min. Then, 500 µl of 1% RBCs was added to the treated virus solution and incubated at RT for 30 min, and the RBC pellet was washed and resuspended in PBS. (E) The amount of RBC-bound virus was determined by qRT-PCR. Data are presented as the mean ± SEM of three replicates and compared by one-way ANOVA followed by Dunnett’s multiple comparisons test (ns, non-significant; * p
    Figure Legend Snippet: Hemagglutination assay using VRE-treated RBCs or viruses. (A) VRE-induced hemagglutination was illustrated. (B) Schematic diagram of the experimental procedures. RBCs were treated with various concentrations of VRE at RT for 30 min. The unbound VRE was removed, and the treated RBC pellet was washed and resuspended. A total of 2,000 HAU of PR8-H1N1 virus was added to the treated RBCs and incubated at RT for 30 min. Then, the RBC pellet was washed again and resuspended in PBS. (C) The amount of RBC-bound virus was determined by qRT-PCR. (D) Schematic diagram of the experimental procedures. A total of 2,000 HAU of PR8-H1N1 virus was treated with various concentrations of VRE or 2 μg of polyclonal antibody against the H1N1 HA protein at RT for 30 min. Then, 500 µl of 1% RBCs was added to the treated virus solution and incubated at RT for 30 min, and the RBC pellet was washed and resuspended in PBS. (E) The amount of RBC-bound virus was determined by qRT-PCR. Data are presented as the mean ± SEM of three replicates and compared by one-way ANOVA followed by Dunnett’s multiple comparisons test (ns, non-significant; * p

    Techniques Used: Hemagglutination Assay, Incubation, Quantitative RT-PCR

    2) Product Images from "Vigna radiata (L.) R. Wilczek Extract Inhibits Influenza A Virus by Targeting Viral Attachment, Penetration, Assembly, and Release"

    Article Title: Vigna radiata (L.) R. Wilczek Extract Inhibits Influenza A Virus by Targeting Viral Attachment, Penetration, Assembly, and Release

    Journal: Frontiers in Pharmacology

    doi: 10.3389/fphar.2020.584973

    Hemagglutination assay using VRE-treated RBCs or viruses. (A) VRE-induced hemagglutination was illustrated. (B) Schematic diagram of the experimental procedures. RBCs were treated with various concentrations of VRE at RT for 30 min. The unbound VRE was removed, and the treated RBC pellet was washed and resuspended. A total of 2,000 HAU of PR8-H1N1 virus was added to the treated RBCs and incubated at RT for 30 min. Then, the RBC pellet was washed again and resuspended in PBS. (C) The amount of RBC-bound virus was determined by qRT-PCR. (D) Schematic diagram of the experimental procedures. A total of 2,000 HAU of PR8-H1N1 virus was treated with various concentrations of VRE or 2 μg of polyclonal antibody against the H1N1 HA protein at RT for 30 min. Then, 500 µl of 1% RBCs was added to the treated virus solution and incubated at RT for 30 min, and the RBC pellet was washed and resuspended in PBS. (E) The amount of RBC-bound virus was determined by qRT-PCR. Data are presented as the mean ± SEM of three replicates and compared by one-way ANOVA followed by Dunnett’s multiple comparisons test (ns, non-significant; * p
    Figure Legend Snippet: Hemagglutination assay using VRE-treated RBCs or viruses. (A) VRE-induced hemagglutination was illustrated. (B) Schematic diagram of the experimental procedures. RBCs were treated with various concentrations of VRE at RT for 30 min. The unbound VRE was removed, and the treated RBC pellet was washed and resuspended. A total of 2,000 HAU of PR8-H1N1 virus was added to the treated RBCs and incubated at RT for 30 min. Then, the RBC pellet was washed again and resuspended in PBS. (C) The amount of RBC-bound virus was determined by qRT-PCR. (D) Schematic diagram of the experimental procedures. A total of 2,000 HAU of PR8-H1N1 virus was treated with various concentrations of VRE or 2 μg of polyclonal antibody against the H1N1 HA protein at RT for 30 min. Then, 500 µl of 1% RBCs was added to the treated virus solution and incubated at RT for 30 min, and the RBC pellet was washed and resuspended in PBS. (E) The amount of RBC-bound virus was determined by qRT-PCR. Data are presented as the mean ± SEM of three replicates and compared by one-way ANOVA followed by Dunnett’s multiple comparisons test (ns, non-significant; * p

    Techniques Used: Hemagglutination Assay, Incubation, Quantitative RT-PCR

    3) Product Images from "Vigna radiata (L.) R. Wilczek Extract Inhibits Influenza A Virus by Targeting Viral Attachment, Penetration, Assembly, and Release"

    Article Title: Vigna radiata (L.) R. Wilczek Extract Inhibits Influenza A Virus by Targeting Viral Attachment, Penetration, Assembly, and Release

    Journal: Frontiers in Pharmacology

    doi: 10.3389/fphar.2020.584973

    Hemagglutination assay using VRE-treated RBCs or viruses. (A) VRE-induced hemagglutination was illustrated. (B) Schematic diagram of the experimental procedures. RBCs were treated with various concentrations of VRE at RT for 30 min. The unbound VRE was removed, and the treated RBC pellet was washed and resuspended. A total of 2,000 HAU of PR8-H1N1 virus was added to the treated RBCs and incubated at RT for 30 min. Then, the RBC pellet was washed again and resuspended in PBS. (C) The amount of RBC-bound virus was determined by qRT-PCR. (D) Schematic diagram of the experimental procedures. A total of 2,000 HAU of PR8-H1N1 virus was treated with various concentrations of VRE or 2 μg of polyclonal antibody against the H1N1 HA protein at RT for 30 min. Then, 500 µl of 1% RBCs was added to the treated virus solution and incubated at RT for 30 min, and the RBC pellet was washed and resuspended in PBS. (E) The amount of RBC-bound virus was determined by qRT-PCR. Data are presented as the mean ± SEM of three replicates and compared by one-way ANOVA followed by Dunnett’s multiple comparisons test (ns, non-significant; * p
    Figure Legend Snippet: Hemagglutination assay using VRE-treated RBCs or viruses. (A) VRE-induced hemagglutination was illustrated. (B) Schematic diagram of the experimental procedures. RBCs were treated with various concentrations of VRE at RT for 30 min. The unbound VRE was removed, and the treated RBC pellet was washed and resuspended. A total of 2,000 HAU of PR8-H1N1 virus was added to the treated RBCs and incubated at RT for 30 min. Then, the RBC pellet was washed again and resuspended in PBS. (C) The amount of RBC-bound virus was determined by qRT-PCR. (D) Schematic diagram of the experimental procedures. A total of 2,000 HAU of PR8-H1N1 virus was treated with various concentrations of VRE or 2 μg of polyclonal antibody against the H1N1 HA protein at RT for 30 min. Then, 500 µl of 1% RBCs was added to the treated virus solution and incubated at RT for 30 min, and the RBC pellet was washed and resuspended in PBS. (E) The amount of RBC-bound virus was determined by qRT-PCR. Data are presented as the mean ± SEM of three replicates and compared by one-way ANOVA followed by Dunnett’s multiple comparisons test (ns, non-significant; * p

    Techniques Used: Hemagglutination Assay, Incubation, Quantitative RT-PCR

    4) Product Images from "Vigna radiata (L.) R. Wilczek Extract Inhibits Influenza A Virus by Targeting Viral Attachment, Penetration, Assembly, and Release"

    Article Title: Vigna radiata (L.) R. Wilczek Extract Inhibits Influenza A Virus by Targeting Viral Attachment, Penetration, Assembly, and Release

    Journal: Frontiers in Pharmacology

    doi: 10.3389/fphar.2020.584973

    VRE interferes with the viral life cycle under different treatment conditions. (A) Schematic diagram of the experimental design. MDCK cells were infected with PR8-H1N1 (MOI = 2) under six different treatment conditions. At 12 hpi, the virus titers were determined by a TCID 50 assay. (B) The viral titers of each group relative to the vehicle control are indicated. Data in the plot present the mean ± SEM of three replicates. Data were compared by one-way ANOVA followed by Dunnett’s multiple comparisons test (**** p
    Figure Legend Snippet: VRE interferes with the viral life cycle under different treatment conditions. (A) Schematic diagram of the experimental design. MDCK cells were infected with PR8-H1N1 (MOI = 2) under six different treatment conditions. At 12 hpi, the virus titers were determined by a TCID 50 assay. (B) The viral titers of each group relative to the vehicle control are indicated. Data in the plot present the mean ± SEM of three replicates. Data were compared by one-way ANOVA followed by Dunnett’s multiple comparisons test (**** p

    Techniques Used: Infection

    VRE inhibits viral neuraminidase activity in a concentration-dependent manner. The PR8-H1N1 neuraminidase inhibition activity of oseltamivir (A) and VRE (B) was measured by detecting fluorescence and normalized to the control. The 3937-H6N1 neuraminidase inhibition activity of oseltamivir (C) and VRE (D) was measured by detecting fluorescence and normalized to the control. The IC 50 for inhibiting neuraminidase is indicated. Data are presented as the mean ± SEM of three replicates. (E) MDCK cells were infected with PR8-H1N1 (MOI = 5) for 1 h, and the cells were treated with VRE (2,000 μg/ml) or oseltamivir (5 μg/ml) for 6–12 h post infection. TEM images demonstrated different levels of virus release from infected MDCK cells.
    Figure Legend Snippet: VRE inhibits viral neuraminidase activity in a concentration-dependent manner. The PR8-H1N1 neuraminidase inhibition activity of oseltamivir (A) and VRE (B) was measured by detecting fluorescence and normalized to the control. The 3937-H6N1 neuraminidase inhibition activity of oseltamivir (C) and VRE (D) was measured by detecting fluorescence and normalized to the control. The IC 50 for inhibiting neuraminidase is indicated. Data are presented as the mean ± SEM of three replicates. (E) MDCK cells were infected with PR8-H1N1 (MOI = 5) for 1 h, and the cells were treated with VRE (2,000 μg/ml) or oseltamivir (5 μg/ml) for 6–12 h post infection. TEM images demonstrated different levels of virus release from infected MDCK cells.

    Techniques Used: Activity Assay, Concentration Assay, Inhibition, Fluorescence, Infection, Transmission Electron Microscopy

    Cytotoxicity and virus inhibitory activity of VRE in MDCK cells. (A) MDCK cells were treated with various concentrations of VRE at 37°C for 24, 48, and 72 h. Cell viability was evaluated by MTT assays. Data in the plot present the mean ± SEM of four replicates. (B) Schematic diagram of the experimental design. PR8-H1N1 (MOI = 0.1) was treated with VRE at 37°C for 1 h. At the same time, MDCK cells were incubated with VRE in medium at 37°C for 1 h. After incubation, the VRE-virus mixture was used to infect cells for 1 h. The cells were washed after supernatant removal and then incubated with VRE for 24 h. The supernatants were collected for the TCID 50 assay. (C) The viral titers of each group are indicated. Data in the plot present the mean ± standard error of the mean (SEM) of three replicates. Data were compared by one-way ANOVA followed by Dunnett’s multiple comparisons test (ns, non-significant; * p
    Figure Legend Snippet: Cytotoxicity and virus inhibitory activity of VRE in MDCK cells. (A) MDCK cells were treated with various concentrations of VRE at 37°C for 24, 48, and 72 h. Cell viability was evaluated by MTT assays. Data in the plot present the mean ± SEM of four replicates. (B) Schematic diagram of the experimental design. PR8-H1N1 (MOI = 0.1) was treated with VRE at 37°C for 1 h. At the same time, MDCK cells were incubated with VRE in medium at 37°C for 1 h. After incubation, the VRE-virus mixture was used to infect cells for 1 h. The cells were washed after supernatant removal and then incubated with VRE for 24 h. The supernatants were collected for the TCID 50 assay. (C) The viral titers of each group are indicated. Data in the plot present the mean ± standard error of the mean (SEM) of three replicates. Data were compared by one-way ANOVA followed by Dunnett’s multiple comparisons test (ns, non-significant; * p

    Techniques Used: Activity Assay, MTT Assay, Incubation

    Hemagglutination assay using VRE-treated RBCs or viruses. (A) VRE-induced hemagglutination was illustrated. (B) Schematic diagram of the experimental procedures. RBCs were treated with various concentrations of VRE at RT for 30 min. The unbound VRE was removed, and the treated RBC pellet was washed and resuspended. A total of 2,000 HAU of PR8-H1N1 virus was added to the treated RBCs and incubated at RT for 30 min. Then, the RBC pellet was washed again and resuspended in PBS. (C) The amount of RBC-bound virus was determined by qRT-PCR. (D) Schematic diagram of the experimental procedures. A total of 2,000 HAU of PR8-H1N1 virus was treated with various concentrations of VRE or 2 μg of polyclonal antibody against the H1N1 HA protein at RT for 30 min. Then, 500 µl of 1% RBCs was added to the treated virus solution and incubated at RT for 30 min, and the RBC pellet was washed and resuspended in PBS. (E) The amount of RBC-bound virus was determined by qRT-PCR. Data are presented as the mean ± SEM of three replicates and compared by one-way ANOVA followed by Dunnett’s multiple comparisons test (ns, non-significant; * p
    Figure Legend Snippet: Hemagglutination assay using VRE-treated RBCs or viruses. (A) VRE-induced hemagglutination was illustrated. (B) Schematic diagram of the experimental procedures. RBCs were treated with various concentrations of VRE at RT for 30 min. The unbound VRE was removed, and the treated RBC pellet was washed and resuspended. A total of 2,000 HAU of PR8-H1N1 virus was added to the treated RBCs and incubated at RT for 30 min. Then, the RBC pellet was washed again and resuspended in PBS. (C) The amount of RBC-bound virus was determined by qRT-PCR. (D) Schematic diagram of the experimental procedures. A total of 2,000 HAU of PR8-H1N1 virus was treated with various concentrations of VRE or 2 μg of polyclonal antibody against the H1N1 HA protein at RT for 30 min. Then, 500 µl of 1% RBCs was added to the treated virus solution and incubated at RT for 30 min, and the RBC pellet was washed and resuspended in PBS. (E) The amount of RBC-bound virus was determined by qRT-PCR. Data are presented as the mean ± SEM of three replicates and compared by one-way ANOVA followed by Dunnett’s multiple comparisons test (ns, non-significant; * p

    Techniques Used: Hemagglutination Assay, Incubation, Quantitative RT-PCR

    Virus inhibition by VRE treatment. (A) Schematic diagram of the experimental design. Different initial time points of VRE treatment of MDCK cells relative to PR8-H1N1 (MOI = 2) infection were tested to evaluate the viral inhibition effect of VRE. (B) At 12 hpi, the extracellular virus titers were determined by TCID 50 . (C) At 12 hpi, intracellular viral RNA was quantitated by qRT-PCR. The untreated cell group was used as a control. Data in the plot present the mean ± SEM of three replicates. Data were compared by one-way ANOVA followed by Dunnett’s multiple comparisons test (ns, non-significant; * p
    Figure Legend Snippet: Virus inhibition by VRE treatment. (A) Schematic diagram of the experimental design. Different initial time points of VRE treatment of MDCK cells relative to PR8-H1N1 (MOI = 2) infection were tested to evaluate the viral inhibition effect of VRE. (B) At 12 hpi, the extracellular virus titers were determined by TCID 50 . (C) At 12 hpi, intracellular viral RNA was quantitated by qRT-PCR. The untreated cell group was used as a control. Data in the plot present the mean ± SEM of three replicates. Data were compared by one-way ANOVA followed by Dunnett’s multiple comparisons test (ns, non-significant; * p

    Techniques Used: Inhibition, Infection, Quantitative RT-PCR

    VRE blocks virus penetration. (A,B) Schematic diagram of the experimental procedures. (C) MDCK cells were infected with PR8-H1N1 (MOI = 2) at 4°C for 1 h for virus attachment. One hour later, VRE was applied to the cells for 1 h to inhibit virus penetration. The unpenetrated virus was inactivated with PBS at pH 2 for 1 min and neutralized with PBS at pH 11. The cells were then incubated in fresh medium for an additional 6 h. The virus signal was detected via immunofluorescence staining with an NP antibody. (D) The fluorescence intensity was measured with a microplate reader. Relative NP expression levels were determined. Data are presented as the mean ± SEM of three replicates and were compared by one-way ANOVA followed by Dunnett’s multiple comparisons test (**** p
    Figure Legend Snippet: VRE blocks virus penetration. (A,B) Schematic diagram of the experimental procedures. (C) MDCK cells were infected with PR8-H1N1 (MOI = 2) at 4°C for 1 h for virus attachment. One hour later, VRE was applied to the cells for 1 h to inhibit virus penetration. The unpenetrated virus was inactivated with PBS at pH 2 for 1 min and neutralized with PBS at pH 11. The cells were then incubated in fresh medium for an additional 6 h. The virus signal was detected via immunofluorescence staining with an NP antibody. (D) The fluorescence intensity was measured with a microplate reader. Relative NP expression levels were determined. Data are presented as the mean ± SEM of three replicates and were compared by one-way ANOVA followed by Dunnett’s multiple comparisons test (**** p

    Techniques Used: Infection, Incubation, Immunofluorescence, Staining, Fluorescence, Expressing

    VRE inhibits α -glucosidase activity in a concentration-dependent manner and interferes with the surface expression of HA. (A) α -Glucosidase activity was measured by detecting the absorbance at 450 nm and normalized to the control. The IC 50 of VRE in inhibiting α -glucosidase is indicated. (B) Schematic diagram of the experimental procedures. MDCK cells were infected with PR8-H1N1 (MOI = 10) and then treated with various concentrations of VRE at 2–5 h post infection. The cells were then washed and fixed with or without permeabilization. HA expression was detected via immunofluorescence staining with an HA antibody. (C) HA expression on the surface (left, cells were fixed only) or in the entire cell (right, cells were fixed and permeabilized) was imaged and merged with DAPI-stained images. (D) Fluorescence intensity was measured. The HA expression levels relative to the nuclear signal were determined from the two different types of staining methods. (E) The ratio of surface/total HA expression under various VRE treatments is shown. Data are presented as the mean ± SEM of three replicates and compared by one-way ANOVA followed by Dunnett’s multiple comparisons test (ns, non-significant; * p
    Figure Legend Snippet: VRE inhibits α -glucosidase activity in a concentration-dependent manner and interferes with the surface expression of HA. (A) α -Glucosidase activity was measured by detecting the absorbance at 450 nm and normalized to the control. The IC 50 of VRE in inhibiting α -glucosidase is indicated. (B) Schematic diagram of the experimental procedures. MDCK cells were infected with PR8-H1N1 (MOI = 10) and then treated with various concentrations of VRE at 2–5 h post infection. The cells were then washed and fixed with or without permeabilization. HA expression was detected via immunofluorescence staining with an HA antibody. (C) HA expression on the surface (left, cells were fixed only) or in the entire cell (right, cells were fixed and permeabilized) was imaged and merged with DAPI-stained images. (D) Fluorescence intensity was measured. The HA expression levels relative to the nuclear signal were determined from the two different types of staining methods. (E) The ratio of surface/total HA expression under various VRE treatments is shown. Data are presented as the mean ± SEM of three replicates and compared by one-way ANOVA followed by Dunnett’s multiple comparisons test (ns, non-significant; * p

    Techniques Used: Activity Assay, Concentration Assay, Expressing, Infection, Immunofluorescence, Staining, Fluorescence

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    Incubation:

    Article Title: ATXN3 Positively Regulates Type I IFN Antiviral Response by Deubiquitinating and Stabilizing HDAC3.
    Article Snippet: .. Membranes were then blocked with 5% nonfat milk or 5% BSA for 0.5 h at room temperature and then incubated with the primary Abs: anti-Flag (F7425; Sigma-Aldrich), anti-HA (ab9110; Abcam), anti-ubiquitin (sc-8017; Santa Cruz Biotechnology), anti-GAPDH (AB-MM001; Hangzhou Goodhere Biotechnology), anti-Myc (M20002H; Abmart), anti–a-tubulin (66031-1-Ig; Proteintech), anti–pY701-STAT1 (9167; Cell Signaling Technology), anti–VSV-G (sc-66180; Santa Cruz Biotechnology), anti–b-actin (66009-1-Ig; Proteintech), anti-HDAC3 (sc-11417; Santa Cruz Biotechnology), anti-ATXN3 (13505-1-AP; Proteintech), and anti-HA (H1N1) (11684-T56; Sino Biological). .. Then the polyvinylidene difluoride membrane was washed three times with PBST (13 PBS and 1% Tween 20) and then subjected to secondary Abs (HRP-conjugated goat anti-rabbit or goat anti-mouse [Bioworld]) for 1 h at room temperature and visualized with ECL Prime (Thermo Scientific).

    Article Title: Vigna radiata (L.) R. Wilczek Extract Inhibits Influenza A Virus by Targeting Viral Attachment, Penetration, Assembly, and Release
    Article Snippet: .. To determine VRE binding to the viral surface and its inhibition of virus-induced hemagglutination, 2,000 HAU of PR8-H1N1 virus was treated with various concentrations (0, 125, 500, and 2,000 μg/ml) of VRE or 2 μg of polyclonal antibody against H1N1 hemagglutinin (SinoBiological #11684-T56) at RT for 30 min. Next, 500 µl of 1% RBCs was added to the treated virus solution and incubated at RT for 30 min, and then the RBC pellet was washed and resuspended in 100 μl of PBS. .. The amount of RBC-bound virus was determined by qRT-PCR.

    Article Title: Vigna radiata (L.) R. Wilczek Extract Inhibits Influenza A Virus by Targeting Viral Attachment, Penetration, Assembly, and Release
    Article Snippet: .. The primary antibody anti-HA (1:500, SinoBiological #11684-T56) was diluted in 1% BSA and incubated with cells overnight at 4°C. .. The secondary antibody, FITC-conjugated goat anti-rabbit IgG (1:1,000, Jackson ImmunoResearch), was diluted with 1% BSA and incubated with cells for 1 h at room temperature.

    Binding Assay:

    Article Title: Vigna radiata (L.) R. Wilczek Extract Inhibits Influenza A Virus by Targeting Viral Attachment, Penetration, Assembly, and Release
    Article Snippet: .. To determine VRE binding to the viral surface and its inhibition of virus-induced hemagglutination, 2,000 HAU of PR8-H1N1 virus was treated with various concentrations (0, 125, 500, and 2,000 μg/ml) of VRE or 2 μg of polyclonal antibody against H1N1 hemagglutinin (SinoBiological #11684-T56) at RT for 30 min. Next, 500 µl of 1% RBCs was added to the treated virus solution and incubated at RT for 30 min, and then the RBC pellet was washed and resuspended in 100 μl of PBS. .. The amount of RBC-bound virus was determined by qRT-PCR.

    Inhibition:

    Article Title: Vigna radiata (L.) R. Wilczek Extract Inhibits Influenza A Virus by Targeting Viral Attachment, Penetration, Assembly, and Release
    Article Snippet: .. To determine VRE binding to the viral surface and its inhibition of virus-induced hemagglutination, 2,000 HAU of PR8-H1N1 virus was treated with various concentrations (0, 125, 500, and 2,000 μg/ml) of VRE or 2 μg of polyclonal antibody against H1N1 hemagglutinin (SinoBiological #11684-T56) at RT for 30 min. Next, 500 µl of 1% RBCs was added to the treated virus solution and incubated at RT for 30 min, and then the RBC pellet was washed and resuspended in 100 μl of PBS. .. The amount of RBC-bound virus was determined by qRT-PCR.

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    Sino Biological influenza a h1n1 hemagglutinin ha antibody rabbit pab
    Hemagglutination assay using VRE-treated RBCs or viruses. (A) VRE-induced hemagglutination was illustrated. (B) Schematic diagram of the experimental procedures. RBCs were treated with various concentrations of VRE at RT for 30 min. The unbound VRE was removed, and the treated RBC pellet was washed and resuspended. A total of 2,000 HAU of <t>PR8-H1N1</t> virus was added to the treated RBCs and incubated at RT for 30 min. Then, the RBC pellet was washed again and resuspended in PBS. (C) The amount of RBC-bound virus was determined by qRT-PCR. (D) Schematic diagram of the experimental procedures. A total of 2,000 HAU of PR8-H1N1 virus was treated with various concentrations of VRE or 2 μg of polyclonal antibody against the H1N1 HA protein at RT for 30 min. Then, 500 µl of 1% RBCs was added to the treated virus solution and incubated at RT for 30 min, and the RBC pellet was washed and resuspended in PBS. (E) The amount of RBC-bound virus was determined by qRT-PCR. Data are presented as the mean ± SEM of three replicates and compared by one-way ANOVA followed by Dunnett’s multiple comparisons test (ns, non-significant; * p
    Influenza A H1n1 Hemagglutinin Ha Antibody Rabbit Pab, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Hemagglutination assay using VRE-treated RBCs or viruses. (A) VRE-induced hemagglutination was illustrated. (B) Schematic diagram of the experimental procedures. RBCs were treated with various concentrations of VRE at RT for 30 min. The unbound VRE was removed, and the treated RBC pellet was washed and resuspended. A total of 2,000 HAU of <t>PR8-H1N1</t> virus was added to the treated RBCs and incubated at RT for 30 min. Then, the RBC pellet was washed again and resuspended in PBS. (C) The amount of RBC-bound virus was determined by qRT-PCR. (D) Schematic diagram of the experimental procedures. A total of 2,000 HAU of PR8-H1N1 virus was treated with various concentrations of VRE or 2 μg of polyclonal antibody against the H1N1 HA protein at RT for 30 min. Then, 500 µl of 1% RBCs was added to the treated virus solution and incubated at RT for 30 min, and the RBC pellet was washed and resuspended in PBS. (E) The amount of RBC-bound virus was determined by qRT-PCR. Data are presented as the mean ± SEM of three replicates and compared by one-way ANOVA followed by Dunnett’s multiple comparisons test (ns, non-significant; * p
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    Hemagglutination assay using VRE-treated RBCs or viruses. (A) VRE-induced hemagglutination was illustrated. (B) Schematic diagram of the experimental procedures. RBCs were treated with various concentrations of VRE at RT for 30 min. The unbound VRE was removed, and the treated RBC pellet was washed and resuspended. A total of 2,000 HAU of PR8-H1N1 virus was added to the treated RBCs and incubated at RT for 30 min. Then, the RBC pellet was washed again and resuspended in PBS. (C) The amount of RBC-bound virus was determined by qRT-PCR. (D) Schematic diagram of the experimental procedures. A total of 2,000 HAU of PR8-H1N1 virus was treated with various concentrations of VRE or 2 μg of polyclonal antibody against the H1N1 HA protein at RT for 30 min. Then, 500 µl of 1% RBCs was added to the treated virus solution and incubated at RT for 30 min, and the RBC pellet was washed and resuspended in PBS. (E) The amount of RBC-bound virus was determined by qRT-PCR. Data are presented as the mean ± SEM of three replicates and compared by one-way ANOVA followed by Dunnett’s multiple comparisons test (ns, non-significant; * p

    Journal: Frontiers in Pharmacology

    Article Title: Vigna radiata (L.) R. Wilczek Extract Inhibits Influenza A Virus by Targeting Viral Attachment, Penetration, Assembly, and Release

    doi: 10.3389/fphar.2020.584973

    Figure Lengend Snippet: Hemagglutination assay using VRE-treated RBCs or viruses. (A) VRE-induced hemagglutination was illustrated. (B) Schematic diagram of the experimental procedures. RBCs were treated with various concentrations of VRE at RT for 30 min. The unbound VRE was removed, and the treated RBC pellet was washed and resuspended. A total of 2,000 HAU of PR8-H1N1 virus was added to the treated RBCs and incubated at RT for 30 min. Then, the RBC pellet was washed again and resuspended in PBS. (C) The amount of RBC-bound virus was determined by qRT-PCR. (D) Schematic diagram of the experimental procedures. A total of 2,000 HAU of PR8-H1N1 virus was treated with various concentrations of VRE or 2 μg of polyclonal antibody against the H1N1 HA protein at RT for 30 min. Then, 500 µl of 1% RBCs was added to the treated virus solution and incubated at RT for 30 min, and the RBC pellet was washed and resuspended in PBS. (E) The amount of RBC-bound virus was determined by qRT-PCR. Data are presented as the mean ± SEM of three replicates and compared by one-way ANOVA followed by Dunnett’s multiple comparisons test (ns, non-significant; * p

    Article Snippet: To determine VRE binding to the viral surface and its inhibition of virus-induced hemagglutination, 2,000 HAU of PR8-H1N1 virus was treated with various concentrations (0, 125, 500, and 2,000 μg/ml) of VRE or 2 μg of polyclonal antibody against H1N1 hemagglutinin (SinoBiological #11684-T56) at RT for 30 min. Next, 500 µl of 1% RBCs was added to the treated virus solution and incubated at RT for 30 min, and then the RBC pellet was washed and resuspended in 100 μl of PBS.

    Techniques: Hemagglutination Assay, Incubation, Quantitative RT-PCR