anti h7n9 ha mouse monoclonal antibody  (Sino Biological)


Bioz Verified Symbol Sino Biological is a verified supplier
Bioz Manufacturer Symbol Sino Biological manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Name:
    Anti H7N9 Hemagglutinin HA Antibody Mouse MAb
    Description:

    Catalog Number:
    11082-MM04
    Price:
    None
    Category:
    Primary Antibody
    Reactivity:
    H7N9
    Applications:
    WB,ELISA
    Antibody Type:
    MAb
    Host:
    Mouse
    Isotype:
    Mouse IgG2b
    Buy from Supplier


    Structured Review

    Sino Biological anti h7n9 ha mouse monoclonal antibody
    Robust HA-specific IgG antibody titers and hemagglutination-inhibition titers in the sera of the immunized mice. (A) IgG antibodies against <t>H7N9</t> A/Shanghai/1/2013 influenza HA protein. (B) IgG antibodies against H7N9 A/Anhui/1/2013 influenza HA protein.

    https://www.bioz.com/result/anti h7n9 ha mouse monoclonal antibody/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti h7n9 ha mouse monoclonal antibody - by Bioz Stars, 2021-04
    94/100 stars

    Images

    1) Product Images from "Protective Immunity to H7N9 Influenza viruses elicited by synthetic DNA Vaccine"

    Article Title: Protective Immunity to H7N9 Influenza viruses elicited by synthetic DNA Vaccine

    Journal: Vaccine

    doi: 10.1016/j.vaccine.2014.02.038

    Robust HA-specific IgG antibody titers and hemagglutination-inhibition titers in the sera of the immunized mice. (A) IgG antibodies against H7N9 A/Shanghai/1/2013 influenza HA protein. (B) IgG antibodies against H7N9 A/Anhui/1/2013 influenza HA protein.
    Figure Legend Snippet: Robust HA-specific IgG antibody titers and hemagglutination-inhibition titers in the sera of the immunized mice. (A) IgG antibodies against H7N9 A/Shanghai/1/2013 influenza HA protein. (B) IgG antibodies against H7N9 A/Anhui/1/2013 influenza HA protein.

    Techniques Used: HI Assay, Mouse Assay

    Protection from H7N9 A/Anhui/1/2013 virus challenge in the immunized mice. (A) Experimental schedule of challenge study. The mice (n=10) were immunized with 25 μg of pH7HA twice, three weeks apart. Four weeks after the second immunization, the
    Figure Legend Snippet: Protection from H7N9 A/Anhui/1/2013 virus challenge in the immunized mice. (A) Experimental schedule of challenge study. The mice (n=10) were immunized with 25 μg of pH7HA twice, three weeks apart. Four weeks after the second immunization, the

    Techniques Used: Mouse Assay

    The H7N9 HA DNA vaccine design and its expression (A) Phylogenetic tree based on neighbor-joining evaluation of H7HA alignment. Asterisks indicate location of consensus sequence. The strains used to generate the consensus HA are indicated. (B) The plasmid
    Figure Legend Snippet: The H7N9 HA DNA vaccine design and its expression (A) Phylogenetic tree based on neighbor-joining evaluation of H7HA alignment. Asterisks indicate location of consensus sequence. The strains used to generate the consensus HA are indicated. (B) The plasmid

    Techniques Used: Expressing, Sequencing, Plasmid Preparation

    2) Product Images from "Protective Immunity to H7N9 Influenza viruses elicited by synthetic DNA Vaccine"

    Article Title: Protective Immunity to H7N9 Influenza viruses elicited by synthetic DNA Vaccine

    Journal: Vaccine

    doi: 10.1016/j.vaccine.2014.02.038

    Robust HA-specific IgG antibody titers and hemagglutination-inhibition titers in the sera of the immunized mice. (A) IgG antibodies against H7N9 A/Shanghai/1/2013 influenza HA protein. (B) IgG antibodies against H7N9 A/Anhui/1/2013 influenza HA protein.
    Figure Legend Snippet: Robust HA-specific IgG antibody titers and hemagglutination-inhibition titers in the sera of the immunized mice. (A) IgG antibodies against H7N9 A/Shanghai/1/2013 influenza HA protein. (B) IgG antibodies against H7N9 A/Anhui/1/2013 influenza HA protein.

    Techniques Used: HI Assay, Mouse Assay

    Protection from H7N9 A/Anhui/1/2013 virus challenge in the immunized mice. (A) Experimental schedule of challenge study. The mice (n=10) were immunized with 25 μg of pH7HA twice, three weeks apart. Four weeks after the second immunization, the
    Figure Legend Snippet: Protection from H7N9 A/Anhui/1/2013 virus challenge in the immunized mice. (A) Experimental schedule of challenge study. The mice (n=10) were immunized with 25 μg of pH7HA twice, three weeks apart. Four weeks after the second immunization, the

    Techniques Used: Mouse Assay

    The H7N9 HA DNA vaccine design and its expression (A) Phylogenetic tree based on neighbor-joining evaluation of H7HA alignment. Asterisks indicate location of consensus sequence. The strains used to generate the consensus HA are indicated. (B) The plasmid
    Figure Legend Snippet: The H7N9 HA DNA vaccine design and its expression (A) Phylogenetic tree based on neighbor-joining evaluation of H7HA alignment. Asterisks indicate location of consensus sequence. The strains used to generate the consensus HA are indicated. (B) The plasmid

    Techniques Used: Expressing, Sequencing, Plasmid Preparation

    3) Product Images from "Nanodiamond enhances immune responses in mice against recombinant HA/H7N9 protein"

    Article Title: Nanodiamond enhances immune responses in mice against recombinant HA/H7N9 protein

    Journal: Journal of Nanobiotechnology

    doi: 10.1186/s12951-017-0305-2

    Immunopotentiation following immunization with an H7:ND (1/12, w/w) conjugate. a Immunization scheme in mice. b Measurement of H7 specific IgG amount in mouse sera via ELISA. In total, 50 ng of purified H7 [Influenza antigen A/Anhui/1/2013 (H7N9), NIBSC] per well were coated at the plate. The sera were diluted 1:5000 and a monoclonal mouse anti- H7N9 haemagglutinin/HA antibody (SinoBiological InC.) at concentrations of 0.5, 0.75, 1, 1.25, 2.5, 5, 12.5, 25, 50, 100, 150 μg/mL was applied as a standard, then analysed via ELISA. Specific immune responses were measured at 450 nm after 1 and 2 booster immunizations with H7 (group 1), H7:ND (group 2) and PBS:ND (group 3). The responses were recalculated according the standard values as µg/mL anti H7N9 antibody in the sera. The BSA background was subtracted. A standard curve was built by the help of OD450 values corresponding to known amounts of H7N9 haemagglutinin/HA antibody. The amount of H7 specific IgG antibody in mouse sera was measured via the standard curve. Statistical analyses were performed using the t-test (SigmaPlot) and are presented. A single dot indicates the value of a single mouse serum. SD was included on a single dot that corresponds to an ELISA data variation of a single mouse serum with three replications. The bars indicate the average value of the test groups. P
    Figure Legend Snippet: Immunopotentiation following immunization with an H7:ND (1/12, w/w) conjugate. a Immunization scheme in mice. b Measurement of H7 specific IgG amount in mouse sera via ELISA. In total, 50 ng of purified H7 [Influenza antigen A/Anhui/1/2013 (H7N9), NIBSC] per well were coated at the plate. The sera were diluted 1:5000 and a monoclonal mouse anti- H7N9 haemagglutinin/HA antibody (SinoBiological InC.) at concentrations of 0.5, 0.75, 1, 1.25, 2.5, 5, 12.5, 25, 50, 100, 150 μg/mL was applied as a standard, then analysed via ELISA. Specific immune responses were measured at 450 nm after 1 and 2 booster immunizations with H7 (group 1), H7:ND (group 2) and PBS:ND (group 3). The responses were recalculated according the standard values as µg/mL anti H7N9 antibody in the sera. The BSA background was subtracted. A standard curve was built by the help of OD450 values corresponding to known amounts of H7N9 haemagglutinin/HA antibody. The amount of H7 specific IgG antibody in mouse sera was measured via the standard curve. Statistical analyses were performed using the t-test (SigmaPlot) and are presented. A single dot indicates the value of a single mouse serum. SD was included on a single dot that corresponds to an ELISA data variation of a single mouse serum with three replications. The bars indicate the average value of the test groups. P

    Techniques Used: Mouse Assay, Enzyme-linked Immunosorbent Assay, Purification

    Construct for expression of trimeric Haemagglutinin in plants. H7 was expressed in tobacco leaves under the control of the CaMV 35S promoter. H7 was fused with trimeric motif GCN4-pII to form H7-pII. Recombinant H7 contained His and c-myc tags for affinity chromatography purification and Western blotting, respectively. The LeB4 signal peptide and KDEL motif were used to ensure ER retention. 35S-P: CaMV 35 S promoter; SP: legumin B4 signal peptide; H7: haemagglutinin A/H7N9; GCN4-pII: trimeric motif; His: His-tag; 35S-T: CaMV 35S terminator
    Figure Legend Snippet: Construct for expression of trimeric Haemagglutinin in plants. H7 was expressed in tobacco leaves under the control of the CaMV 35S promoter. H7 was fused with trimeric motif GCN4-pII to form H7-pII. Recombinant H7 contained His and c-myc tags for affinity chromatography purification and Western blotting, respectively. The LeB4 signal peptide and KDEL motif were used to ensure ER retention. 35S-P: CaMV 35 S promoter; SP: legumin B4 signal peptide; H7: haemagglutinin A/H7N9; GCN4-pII: trimeric motif; His: His-tag; 35S-T: CaMV 35S terminator

    Techniques Used: Construct, Expressing, Recombinant, Affinity Chromatography, Purification, Western Blot

    4) Product Images from "Nanodiamond enhances immune responses in mice against recombinant HA/H7N9 protein"

    Article Title: Nanodiamond enhances immune responses in mice against recombinant HA/H7N9 protein

    Journal: Journal of Nanobiotechnology

    doi: 10.1186/s12951-017-0305-2

    Immunopotentiation following immunization with an H7:ND (1/12, w/w) conjugate. a Immunization scheme in mice. b Measurement of H7 specific IgG amount in mouse sera via ELISA. In total, 50 ng of purified H7 [Influenza antigen A/Anhui/1/2013 (H7N9), NIBSC] per well were coated at the plate. The sera were diluted 1:5000 and a monoclonal mouse anti- H7N9 haemagglutinin/HA antibody (SinoBiological InC.) at concentrations of 0.5, 0.75, 1, 1.25, 2.5, 5, 12.5, 25, 50, 100, 150 μg/mL was applied as a standard, then analysed via ELISA. Specific immune responses were measured at 450 nm after 1 and 2 booster immunizations with H7 (group 1), H7:ND (group 2) and PBS:ND (group 3). The responses were recalculated according the standard values as µg/mL anti H7N9 antibody in the sera. The BSA background was subtracted. A standard curve was built by the help of OD450 values corresponding to known amounts of H7N9 haemagglutinin/HA antibody. The amount of H7 specific IgG antibody in mouse sera was measured via the standard curve. Statistical analyses were performed using the t-test (SigmaPlot) and are presented. A single dot indicates the value of a single mouse serum. SD was included on a single dot that corresponds to an ELISA data variation of a single mouse serum with three replications. The bars indicate the average value of the test groups. P
    Figure Legend Snippet: Immunopotentiation following immunization with an H7:ND (1/12, w/w) conjugate. a Immunization scheme in mice. b Measurement of H7 specific IgG amount in mouse sera via ELISA. In total, 50 ng of purified H7 [Influenza antigen A/Anhui/1/2013 (H7N9), NIBSC] per well were coated at the plate. The sera were diluted 1:5000 and a monoclonal mouse anti- H7N9 haemagglutinin/HA antibody (SinoBiological InC.) at concentrations of 0.5, 0.75, 1, 1.25, 2.5, 5, 12.5, 25, 50, 100, 150 μg/mL was applied as a standard, then analysed via ELISA. Specific immune responses were measured at 450 nm after 1 and 2 booster immunizations with H7 (group 1), H7:ND (group 2) and PBS:ND (group 3). The responses were recalculated according the standard values as µg/mL anti H7N9 antibody in the sera. The BSA background was subtracted. A standard curve was built by the help of OD450 values corresponding to known amounts of H7N9 haemagglutinin/HA antibody. The amount of H7 specific IgG antibody in mouse sera was measured via the standard curve. Statistical analyses were performed using the t-test (SigmaPlot) and are presented. A single dot indicates the value of a single mouse serum. SD was included on a single dot that corresponds to an ELISA data variation of a single mouse serum with three replications. The bars indicate the average value of the test groups. P

    Techniques Used: Mouse Assay, Enzyme-linked Immunosorbent Assay, Purification

    5) Product Images from "Nanodiamond enhances immune responses in mice against recombinant HA/H7N9 protein"

    Article Title: Nanodiamond enhances immune responses in mice against recombinant HA/H7N9 protein

    Journal: Journal of Nanobiotechnology

    doi: 10.1186/s12951-017-0305-2

    Immunopotentiation following immunization with an H7:ND (1/12, w/w) conjugate. a Immunization scheme in mice. b Measurement of H7 specific IgG amount in mouse sera via ELISA. In total, 50 ng of purified H7 [Influenza antigen A/Anhui/1/2013 (H7N9), NIBSC] per well were coated at the plate. The sera were diluted 1:5000 and a monoclonal mouse anti- H7N9 haemagglutinin/HA antibody (SinoBiological InC.) at concentrations of 0.5, 0.75, 1, 1.25, 2.5, 5, 12.5, 25, 50, 100, 150 μg/mL was applied as a standard, then analysed via ELISA. Specific immune responses were measured at 450 nm after 1 and 2 booster immunizations with H7 (group 1), H7:ND (group 2) and PBS:ND (group 3). The responses were recalculated according the standard values as µg/mL anti H7N9 antibody in the sera. The BSA background was subtracted. A standard curve was built by the help of OD450 values corresponding to known amounts of H7N9 haemagglutinin/HA antibody. The amount of H7 specific IgG antibody in mouse sera was measured via the standard curve. Statistical analyses were performed using the t-test (SigmaPlot) and are presented. A single dot indicates the value of a single mouse serum. SD was included on a single dot that corresponds to an ELISA data variation of a single mouse serum with three replications. The bars indicate the average value of the test groups. P
    Figure Legend Snippet: Immunopotentiation following immunization with an H7:ND (1/12, w/w) conjugate. a Immunization scheme in mice. b Measurement of H7 specific IgG amount in mouse sera via ELISA. In total, 50 ng of purified H7 [Influenza antigen A/Anhui/1/2013 (H7N9), NIBSC] per well were coated at the plate. The sera were diluted 1:5000 and a monoclonal mouse anti- H7N9 haemagglutinin/HA antibody (SinoBiological InC.) at concentrations of 0.5, 0.75, 1, 1.25, 2.5, 5, 12.5, 25, 50, 100, 150 μg/mL was applied as a standard, then analysed via ELISA. Specific immune responses were measured at 450 nm after 1 and 2 booster immunizations with H7 (group 1), H7:ND (group 2) and PBS:ND (group 3). The responses were recalculated according the standard values as µg/mL anti H7N9 antibody in the sera. The BSA background was subtracted. A standard curve was built by the help of OD450 values corresponding to known amounts of H7N9 haemagglutinin/HA antibody. The amount of H7 specific IgG antibody in mouse sera was measured via the standard curve. Statistical analyses were performed using the t-test (SigmaPlot) and are presented. A single dot indicates the value of a single mouse serum. SD was included on a single dot that corresponds to an ELISA data variation of a single mouse serum with three replications. The bars indicate the average value of the test groups. P

    Techniques Used: Mouse Assay, Enzyme-linked Immunosorbent Assay, Purification

    Construct for expression of trimeric Haemagglutinin in plants. H7 was expressed in tobacco leaves under the control of the CaMV 35S promoter. H7 was fused with trimeric motif GCN4-pII to form H7-pII. Recombinant H7 contained His and c-myc tags for affinity chromatography purification and Western blotting, respectively. The LeB4 signal peptide and KDEL motif were used to ensure ER retention. 35S-P: CaMV 35 S promoter; SP: legumin B4 signal peptide; H7: haemagglutinin A/H7N9; GCN4-pII: trimeric motif; His: His-tag; 35S-T: CaMV 35S terminator
    Figure Legend Snippet: Construct for expression of trimeric Haemagglutinin in plants. H7 was expressed in tobacco leaves under the control of the CaMV 35S promoter. H7 was fused with trimeric motif GCN4-pII to form H7-pII. Recombinant H7 contained His and c-myc tags for affinity chromatography purification and Western blotting, respectively. The LeB4 signal peptide and KDEL motif were used to ensure ER retention. 35S-P: CaMV 35 S promoter; SP: legumin B4 signal peptide; H7: haemagglutinin A/H7N9; GCN4-pII: trimeric motif; His: His-tag; 35S-T: CaMV 35S terminator

    Techniques Used: Construct, Expressing, Recombinant, Affinity Chromatography, Purification, Western Blot

    Related Articles

    Blocking Assay:

    Article Title: Antiviral activity of carnosic acid against respiratory syncytial virus
    Article Snippet: Final concentration of DMSO was 2% in all samples. .. After blocking with 5% skim milk, the expression of hRSV fusion (F) protein was assessed by enzyme-linked immune sorbent assay (ELISA) using mouse anti-F monoclonal antibody (Sino Biological) and horseradish peroxidase (HRP)-conjugated anti-mouse IgG. .. Extraction and isolation of compounds Air-dried, powdered aerial parts of R. officinalis (12.5 g) were extracted three times with 70% ethanol (EtOH) by maceration.

    Article Title: Nanodiamond enhances immune responses in mice against recombinant HA/H7N9 protein
    Article Snippet: ELISA For testing mouse sera, microtiter plates (ImmunoPlate Maxisorp, Nalgen Nunc International, Roskilde, Denmark) were coated with 100 µL of 50 ng H7 (Influenza Antigen A/Anhui/1/2013 (H7N9), NIBSC) in PBS (100 mM NaCl, 32 mM Na2 HPO4 , 17 mM Na2 HPO4, pH 7.2) and incubated overnight. .. After blocking with 3% (w/v) bovine serum albumin (BSA), 0.05% (v/v) Tween20 in PBS (PBST) for 2 h, 100 µL of the specific mouse sera dilution [5000 times in 1% (w/v) BSA in PBST] and an anti H7N9 haemagglutinin/HA monoclonal mouse antibody (SinoBiological InC.) at concentrations of 0.5, 0.75, 1, 1.25, 2.5, 5, 12.5, 25, 50, 100, 150 μg/mL was applied on each ELISA plate and incubated at 25 °C for 1 h. Each serum dilution was measured in triplicate. .. The plates were washed 5 times with PBST, followed by the addition of 100 µl of a goat anti-mouse IgG conjugate horseradish peroxidase (HRP) dilution (2000 times in 1% (w/v) BSA in PBST and then incubated at 25 °C for 1 h. The enzymatic substrate, 1-Step™ Ultra TMB-ELISA Substrate Solution (Thermo Fisher Scientific, Lithuania) was added.

    Expressing:

    Article Title: Antiviral activity of carnosic acid against respiratory syncytial virus
    Article Snippet: Final concentration of DMSO was 2% in all samples. .. After blocking with 5% skim milk, the expression of hRSV fusion (F) protein was assessed by enzyme-linked immune sorbent assay (ELISA) using mouse anti-F monoclonal antibody (Sino Biological) and horseradish peroxidase (HRP)-conjugated anti-mouse IgG. .. Extraction and isolation of compounds Air-dried, powdered aerial parts of R. officinalis (12.5 g) were extracted three times with 70% ethanol (EtOH) by maceration.

    Article Title: Protective Immunity to H7N9 Influenza viruses elicited by synthetic DNA Vaccine
    Article Snippet: The synthetic H7HA gene, which is 1683 bp in length, was synthesized, sequence verified, and was subcloned into the expression vector pGX0001 at BamHI and NotI sites and named pH7HA ( ) in just a few days. .. To determine the expression of the H7HA construct, an indirect immunofluorescent assay was carried out using an anti-H7N9 HA mouse monoclonal antibody. .. High membrane expression was observed by fluorescent microscopy in the pH7HA-transfected cells , supporting the idea that the expressed HA protein was a surface protein and the protein exhibited a relatively native conformation.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Antiviral activity of carnosic acid against respiratory syncytial virus
    Article Snippet: Final concentration of DMSO was 2% in all samples. .. After blocking with 5% skim milk, the expression of hRSV fusion (F) protein was assessed by enzyme-linked immune sorbent assay (ELISA) using mouse anti-F monoclonal antibody (Sino Biological) and horseradish peroxidase (HRP)-conjugated anti-mouse IgG. .. Extraction and isolation of compounds Air-dried, powdered aerial parts of R. officinalis (12.5 g) were extracted three times with 70% ethanol (EtOH) by maceration.

    Article Title: Nanodiamond enhances immune responses in mice against recombinant HA/H7N9 protein
    Article Snippet: ELISA For testing mouse sera, microtiter plates (ImmunoPlate Maxisorp, Nalgen Nunc International, Roskilde, Denmark) were coated with 100 µL of 50 ng H7 (Influenza Antigen A/Anhui/1/2013 (H7N9), NIBSC) in PBS (100 mM NaCl, 32 mM Na2 HPO4 , 17 mM Na2 HPO4, pH 7.2) and incubated overnight. .. After blocking with 3% (w/v) bovine serum albumin (BSA), 0.05% (v/v) Tween20 in PBS (PBST) for 2 h, 100 µL of the specific mouse sera dilution [5000 times in 1% (w/v) BSA in PBST] and an anti H7N9 haemagglutinin/HA monoclonal mouse antibody (SinoBiological InC.) at concentrations of 0.5, 0.75, 1, 1.25, 2.5, 5, 12.5, 25, 50, 100, 150 μg/mL was applied on each ELISA plate and incubated at 25 °C for 1 h. Each serum dilution was measured in triplicate. .. The plates were washed 5 times with PBST, followed by the addition of 100 µl of a goat anti-mouse IgG conjugate horseradish peroxidase (HRP) dilution (2000 times in 1% (w/v) BSA in PBST and then incubated at 25 °C for 1 h. The enzymatic substrate, 1-Step™ Ultra TMB-ELISA Substrate Solution (Thermo Fisher Scientific, Lithuania) was added.

    Article Title: Nanodiamond enhances immune responses in mice against recombinant HA/H7N9 protein
    Article Snippet: .. The ELISA values of all mouse sera were normalised by use of a monoclonal mouse standard H7N9 haemagglutinin/HA antibody (SinoBiological Inc.). ..

    SDS Page:

    Article Title: Identifying novel amino acid substitutions of hemagglutinin involved in virulence enhancement in H7N9 virus strains
    Article Snippet: Western Blot Cultured cells were washed with PBS twice, lysed with lysis buffer (20 mM Tris, pH 8.0, 0.5% NP-40, 0.25% sodium deoxycholate, 1 mM EDTA, protease inhibitor cocktail), centrifuged at 15,000g for 15 min, and supernatants were collected for protein quantification by the Lowry’s method. .. After diluting the samples to 2 mg/mL, proteins were heat-denatured for 5 min, submitted to 4–10% SDS-PAGE, transferred onto a PVDF membrane, blocked with 1% bovine serum albumin, and incubated with H7 monoclonal antibody (1000; mouse anti-H7N9 hemagglutinin/HA antibody, Sino Biological, Beijing) at 4 °C overnight. .. The membrane was then incubated with anti-mouse IR dye 800-labeled IgG secondary antibody (Li-Cor, Lincoln, NE), measured at a recommended wavelength, and analyzed by the Odyssey software.

    Incubation:

    Article Title: Identifying novel amino acid substitutions of hemagglutinin involved in virulence enhancement in H7N9 virus strains
    Article Snippet: Western Blot Cultured cells were washed with PBS twice, lysed with lysis buffer (20 mM Tris, pH 8.0, 0.5% NP-40, 0.25% sodium deoxycholate, 1 mM EDTA, protease inhibitor cocktail), centrifuged at 15,000g for 15 min, and supernatants were collected for protein quantification by the Lowry’s method. .. After diluting the samples to 2 mg/mL, proteins were heat-denatured for 5 min, submitted to 4–10% SDS-PAGE, transferred onto a PVDF membrane, blocked with 1% bovine serum albumin, and incubated with H7 monoclonal antibody (1000; mouse anti-H7N9 hemagglutinin/HA antibody, Sino Biological, Beijing) at 4 °C overnight. .. The membrane was then incubated with anti-mouse IR dye 800-labeled IgG secondary antibody (Li-Cor, Lincoln, NE), measured at a recommended wavelength, and analyzed by the Odyssey software.

    Article Title: Protective Immunity to H7N9 Influenza viruses elicited by synthetic DNA Vaccine
    Article Snippet: .. The cells were incubated with anti-H7N9 HA mouse monoclonal antibody (Sino Biological Inc., Cat# 11082-MM04) at a 1:400 dilution for 2h at room temperature. .. The slides were then incubated with the Alexa 555-conjugated anti-mouse secondary antibody (Cell Signaling Technology) for 60 min in the dark, and analyzed by fluorescent microscopy (Leica DM4000B, Leica Microsystems Inc, USA) using the SPOT Advanced software program (SPOT™ Diagnostic Instruments, Inc).

    Article Title: Nanodiamond enhances immune responses in mice against recombinant HA/H7N9 protein
    Article Snippet: ELISA For testing mouse sera, microtiter plates (ImmunoPlate Maxisorp, Nalgen Nunc International, Roskilde, Denmark) were coated with 100 µL of 50 ng H7 (Influenza Antigen A/Anhui/1/2013 (H7N9), NIBSC) in PBS (100 mM NaCl, 32 mM Na2 HPO4 , 17 mM Na2 HPO4, pH 7.2) and incubated overnight. .. After blocking with 3% (w/v) bovine serum albumin (BSA), 0.05% (v/v) Tween20 in PBS (PBST) for 2 h, 100 µL of the specific mouse sera dilution [5000 times in 1% (w/v) BSA in PBST] and an anti H7N9 haemagglutinin/HA monoclonal mouse antibody (SinoBiological InC.) at concentrations of 0.5, 0.75, 1, 1.25, 2.5, 5, 12.5, 25, 50, 100, 150 μg/mL was applied on each ELISA plate and incubated at 25 °C for 1 h. Each serum dilution was measured in triplicate. .. The plates were washed 5 times with PBST, followed by the addition of 100 µl of a goat anti-mouse IgG conjugate horseradish peroxidase (HRP) dilution (2000 times in 1% (w/v) BSA in PBST and then incubated at 25 °C for 1 h. The enzymatic substrate, 1-Step™ Ultra TMB-ELISA Substrate Solution (Thermo Fisher Scientific, Lithuania) was added.

    Positive Control:

    Article Title: Nanodiamond enhances immune responses in mice against recombinant HA/H7N9 protein
    Article Snippet: Then, the single stripes were incubated for 2 h at room temperature with a 1:500 dilution of mixture of five mice sera of each group (group 1, group 2 and group 3). .. Anti-mouse H7N9 Haemagglutinin/HA antibody (SinoBiological InC., China) was used as a positive control (P). .. Next, the membranes were incubated for 1 h at room temperature with the addition of a 1∶2000 dilution of an HRP conjugated goat anti-mouse IgG secondary antibody.

    Construct:

    Article Title: Protective Immunity to H7N9 Influenza viruses elicited by synthetic DNA Vaccine
    Article Snippet: The synthetic H7HA gene, which is 1683 bp in length, was synthesized, sequence verified, and was subcloned into the expression vector pGX0001 at BamHI and NotI sites and named pH7HA ( ) in just a few days. .. To determine the expression of the H7HA construct, an indirect immunofluorescent assay was carried out using an anti-H7N9 HA mouse monoclonal antibody. .. High membrane expression was observed by fluorescent microscopy in the pH7HA-transfected cells , supporting the idea that the expressed HA protein was a surface protein and the protein exhibited a relatively native conformation.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Sino Biological anti h7n9 ha mouse monoclonal antibody
    Robust HA-specific IgG antibody titers and hemagglutination-inhibition titers in the sera of the immunized mice. (A) IgG antibodies against <t>H7N9</t> A/Shanghai/1/2013 influenza HA protein. (B) IgG antibodies against H7N9 A/Anhui/1/2013 influenza HA protein.
    Anti H7n9 Ha Mouse Monoclonal Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti h7n9 ha mouse monoclonal antibody/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti h7n9 ha mouse monoclonal antibody - by Bioz Stars, 2021-04
    94/100 stars
      Buy from Supplier

    Image Search Results


    Robust HA-specific IgG antibody titers and hemagglutination-inhibition titers in the sera of the immunized mice. (A) IgG antibodies against H7N9 A/Shanghai/1/2013 influenza HA protein. (B) IgG antibodies against H7N9 A/Anhui/1/2013 influenza HA protein.

    Journal: Vaccine

    Article Title: Protective Immunity to H7N9 Influenza viruses elicited by synthetic DNA Vaccine

    doi: 10.1016/j.vaccine.2014.02.038

    Figure Lengend Snippet: Robust HA-specific IgG antibody titers and hemagglutination-inhibition titers in the sera of the immunized mice. (A) IgG antibodies against H7N9 A/Shanghai/1/2013 influenza HA protein. (B) IgG antibodies against H7N9 A/Anhui/1/2013 influenza HA protein.

    Article Snippet: The cells were incubated with anti-H7N9 HA mouse monoclonal antibody (Sino Biological Inc., Cat# 11082-MM04) at a 1:400 dilution for 2h at room temperature.

    Techniques: HI Assay, Mouse Assay

    Protection from H7N9 A/Anhui/1/2013 virus challenge in the immunized mice. (A) Experimental schedule of challenge study. The mice (n=10) were immunized with 25 μg of pH7HA twice, three weeks apart. Four weeks after the second immunization, the

    Journal: Vaccine

    Article Title: Protective Immunity to H7N9 Influenza viruses elicited by synthetic DNA Vaccine

    doi: 10.1016/j.vaccine.2014.02.038

    Figure Lengend Snippet: Protection from H7N9 A/Anhui/1/2013 virus challenge in the immunized mice. (A) Experimental schedule of challenge study. The mice (n=10) were immunized with 25 μg of pH7HA twice, three weeks apart. Four weeks after the second immunization, the

    Article Snippet: The cells were incubated with anti-H7N9 HA mouse monoclonal antibody (Sino Biological Inc., Cat# 11082-MM04) at a 1:400 dilution for 2h at room temperature.

    Techniques: Mouse Assay

    The H7N9 HA DNA vaccine design and its expression (A) Phylogenetic tree based on neighbor-joining evaluation of H7HA alignment. Asterisks indicate location of consensus sequence. The strains used to generate the consensus HA are indicated. (B) The plasmid

    Journal: Vaccine

    Article Title: Protective Immunity to H7N9 Influenza viruses elicited by synthetic DNA Vaccine

    doi: 10.1016/j.vaccine.2014.02.038

    Figure Lengend Snippet: The H7N9 HA DNA vaccine design and its expression (A) Phylogenetic tree based on neighbor-joining evaluation of H7HA alignment. Asterisks indicate location of consensus sequence. The strains used to generate the consensus HA are indicated. (B) The plasmid

    Article Snippet: The cells were incubated with anti-H7N9 HA mouse monoclonal antibody (Sino Biological Inc., Cat# 11082-MM04) at a 1:400 dilution for 2h at room temperature.

    Techniques: Expressing, Sequencing, Plasmid Preparation

    Immunopotentiation following immunization with an H7:ND (1/12, w/w) conjugate. a Immunization scheme in mice. b Measurement of H7 specific IgG amount in mouse sera via ELISA. In total, 50 ng of purified H7 [Influenza antigen A/Anhui/1/2013 (H7N9), NIBSC] per well were coated at the plate. The sera were diluted 1:5000 and a monoclonal mouse anti- H7N9 haemagglutinin/HA antibody (SinoBiological InC.) at concentrations of 0.5, 0.75, 1, 1.25, 2.5, 5, 12.5, 25, 50, 100, 150 μg/mL was applied as a standard, then analysed via ELISA. Specific immune responses were measured at 450 nm after 1 and 2 booster immunizations with H7 (group 1), H7:ND (group 2) and PBS:ND (group 3). The responses were recalculated according the standard values as µg/mL anti H7N9 antibody in the sera. The BSA background was subtracted. A standard curve was built by the help of OD450 values corresponding to known amounts of H7N9 haemagglutinin/HA antibody. The amount of H7 specific IgG antibody in mouse sera was measured via the standard curve. Statistical analyses were performed using the t-test (SigmaPlot) and are presented. A single dot indicates the value of a single mouse serum. SD was included on a single dot that corresponds to an ELISA data variation of a single mouse serum with three replications. The bars indicate the average value of the test groups. P

    Journal: Journal of Nanobiotechnology

    Article Title: Nanodiamond enhances immune responses in mice against recombinant HA/H7N9 protein

    doi: 10.1186/s12951-017-0305-2

    Figure Lengend Snippet: Immunopotentiation following immunization with an H7:ND (1/12, w/w) conjugate. a Immunization scheme in mice. b Measurement of H7 specific IgG amount in mouse sera via ELISA. In total, 50 ng of purified H7 [Influenza antigen A/Anhui/1/2013 (H7N9), NIBSC] per well were coated at the plate. The sera were diluted 1:5000 and a monoclonal mouse anti- H7N9 haemagglutinin/HA antibody (SinoBiological InC.) at concentrations of 0.5, 0.75, 1, 1.25, 2.5, 5, 12.5, 25, 50, 100, 150 μg/mL was applied as a standard, then analysed via ELISA. Specific immune responses were measured at 450 nm after 1 and 2 booster immunizations with H7 (group 1), H7:ND (group 2) and PBS:ND (group 3). The responses were recalculated according the standard values as µg/mL anti H7N9 antibody in the sera. The BSA background was subtracted. A standard curve was built by the help of OD450 values corresponding to known amounts of H7N9 haemagglutinin/HA antibody. The amount of H7 specific IgG antibody in mouse sera was measured via the standard curve. Statistical analyses were performed using the t-test (SigmaPlot) and are presented. A single dot indicates the value of a single mouse serum. SD was included on a single dot that corresponds to an ELISA data variation of a single mouse serum with three replications. The bars indicate the average value of the test groups. P

    Article Snippet: After blocking with 3% (w/v) bovine serum albumin (BSA), 0.05% (v/v) Tween20 in PBS (PBST) for 2 h, 100 µL of the specific mouse sera dilution [5000 times in 1% (w/v) BSA in PBST] and an anti H7N9 haemagglutinin/HA monoclonal mouse antibody (SinoBiological InC.) at concentrations of 0.5, 0.75, 1, 1.25, 2.5, 5, 12.5, 25, 50, 100, 150 μg/mL was applied on each ELISA plate and incubated at 25 °C for 1 h. Each serum dilution was measured in triplicate.

    Techniques: Mouse Assay, Enzyme-linked Immunosorbent Assay, Purification

    Construct for expression of trimeric Haemagglutinin in plants. H7 was expressed in tobacco leaves under the control of the CaMV 35S promoter. H7 was fused with trimeric motif GCN4-pII to form H7-pII. Recombinant H7 contained His and c-myc tags for affinity chromatography purification and Western blotting, respectively. The LeB4 signal peptide and KDEL motif were used to ensure ER retention. 35S-P: CaMV 35 S promoter; SP: legumin B4 signal peptide; H7: haemagglutinin A/H7N9; GCN4-pII: trimeric motif; His: His-tag; 35S-T: CaMV 35S terminator

    Journal: Journal of Nanobiotechnology

    Article Title: Nanodiamond enhances immune responses in mice against recombinant HA/H7N9 protein

    doi: 10.1186/s12951-017-0305-2

    Figure Lengend Snippet: Construct for expression of trimeric Haemagglutinin in plants. H7 was expressed in tobacco leaves under the control of the CaMV 35S promoter. H7 was fused with trimeric motif GCN4-pII to form H7-pII. Recombinant H7 contained His and c-myc tags for affinity chromatography purification and Western blotting, respectively. The LeB4 signal peptide and KDEL motif were used to ensure ER retention. 35S-P: CaMV 35 S promoter; SP: legumin B4 signal peptide; H7: haemagglutinin A/H7N9; GCN4-pII: trimeric motif; His: His-tag; 35S-T: CaMV 35S terminator

    Article Snippet: After blocking with 3% (w/v) bovine serum albumin (BSA), 0.05% (v/v) Tween20 in PBS (PBST) for 2 h, 100 µL of the specific mouse sera dilution [5000 times in 1% (w/v) BSA in PBST] and an anti H7N9 haemagglutinin/HA monoclonal mouse antibody (SinoBiological InC.) at concentrations of 0.5, 0.75, 1, 1.25, 2.5, 5, 12.5, 25, 50, 100, 150 μg/mL was applied on each ELISA plate and incubated at 25 °C for 1 h. Each serum dilution was measured in triplicate.

    Techniques: Construct, Expressing, Recombinant, Affinity Chromatography, Purification, Western Blot