influenza a h5n1 ha protein  (Sino Biological)


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    Sino Biological influenza a h5n1 ha protein
    Competition-mediated detection of test epitope presentation on MHC-II. a F24 staining of 1055 HLA-DRB1*15:01 and 9036 *11:01 pulsed with p24 (reference epitope) alone or with an increasing concentration of HA test antigen (test peptide). b F11 staining of 1111 HLA-DRB1*04:01 and 1124 *01:01 cells pulsed with HA (reference epitope) alone or with an increasing concentration p24 test antigen (test peptide) ( c , d ). F24 staining of 9036 HLA-DRB1*11:01 and 1055 *15:01 cells was pulsed with p24 alone or in the presence of Omomyc ( c ) or <t>influenza</t> A <t>H5N1</t> HA protein ( d ). The data on Y -axis is expressed as the percent sTCR florescence signal (MFI) relative to cells pulsed with reference epitope: MFI (test compound + reference epitope) / median MFI (reference epitope alone). The one-way ANOVA with multiple comparisons was used to determine significance, p > 0.05 not significant ( ns ). For statistical comparisons in a and b , samples treated with test peptide were compared to no test epitope; for c and d , samples treated with reference peptide + protein were compared to the reference epitope alone
    Influenza A H5n1 Ha Protein, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/influenza a h5n1 ha protein/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    influenza a h5n1 ha protein - by Bioz Stars, 2021-06
    94/100 stars

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    1) Product Images from "Competition-Based Cell Assay Employing Soluble T Cell Receptors to Assess MHC Class II Antigen Processing and Presentation"

    Article Title: Competition-Based Cell Assay Employing Soluble T Cell Receptors to Assess MHC Class II Antigen Processing and Presentation

    Journal: The AAPS Journal

    doi: 10.1208/s12248-020-00553-x

    Competition-mediated detection of test epitope presentation on MHC-II. a F24 staining of 1055 HLA-DRB1*15:01 and 9036 *11:01 pulsed with p24 (reference epitope) alone or with an increasing concentration of HA test antigen (test peptide). b F11 staining of 1111 HLA-DRB1*04:01 and 1124 *01:01 cells pulsed with HA (reference epitope) alone or with an increasing concentration p24 test antigen (test peptide) ( c , d ). F24 staining of 9036 HLA-DRB1*11:01 and 1055 *15:01 cells was pulsed with p24 alone or in the presence of Omomyc ( c ) or influenza A H5N1 HA protein ( d ). The data on Y -axis is expressed as the percent sTCR florescence signal (MFI) relative to cells pulsed with reference epitope: MFI (test compound + reference epitope) / median MFI (reference epitope alone). The one-way ANOVA with multiple comparisons was used to determine significance, p > 0.05 not significant ( ns ). For statistical comparisons in a and b , samples treated with test peptide were compared to no test epitope; for c and d , samples treated with reference peptide + protein were compared to the reference epitope alone
    Figure Legend Snippet: Competition-mediated detection of test epitope presentation on MHC-II. a F24 staining of 1055 HLA-DRB1*15:01 and 9036 *11:01 pulsed with p24 (reference epitope) alone or with an increasing concentration of HA test antigen (test peptide). b F11 staining of 1111 HLA-DRB1*04:01 and 1124 *01:01 cells pulsed with HA (reference epitope) alone or with an increasing concentration p24 test antigen (test peptide) ( c , d ). F24 staining of 9036 HLA-DRB1*11:01 and 1055 *15:01 cells was pulsed with p24 alone or in the presence of Omomyc ( c ) or influenza A H5N1 HA protein ( d ). The data on Y -axis is expressed as the percent sTCR florescence signal (MFI) relative to cells pulsed with reference epitope: MFI (test compound + reference epitope) / median MFI (reference epitope alone). The one-way ANOVA with multiple comparisons was used to determine significance, p > 0.05 not significant ( ns ). For statistical comparisons in a and b , samples treated with test peptide were compared to no test epitope; for c and d , samples treated with reference peptide + protein were compared to the reference epitope alone

    Techniques Used: Staining, Concentration Assay

    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Generation and testing anti-influenza human monoclonal antibodies in a new humanized mouse model (DRAGA: HLA-A2. HLA-DR4. Rag1 KO. IL-2Rγc KO. NOD)
    Article Snippet: Gels were stained with Amido Black for 30 min at room temperature, dried under hot air blower, and de-stained as per manufacturer's instructions. .. ELISA was used to select positive hybridoma clones secreting anti-HA hu-mAbs and to isotype these antibodies, to determine the cross-reactivity of hu-mAbs to rHAs from various influenza virus heterotypes, to measure the BALB/c mouse Ab response to anti-HA hu-mAb treatment and to PR8 virus infection, and to determine the span-life of 16D11 anti-HA hu-mAb in the blood circulation of BALB/c mice; (i) Selection of stable hybridoma clones secreting anti-HA hu-mAbs in the cell culture supernatant and isotyping the affinity-purified hu-mAbs by these clones was carried out by semi-quantitative ELISA kits (Bethyl Laboratories) according to the manufacturer's instructions; (ii) Cross-reactivity of hu-mAbs to rHAs from various influenza virus heterotypes was measured in 96-well plates were coated overnight at 4°C with 20 μg/ml of recombinant HA proteins from A/Puerto Rico/8/1934 (Cat# 11684-V08H), A/WSN/1933 (Cat# 11692-V08H), A/Aichi/2/1968 (Cat# 11707-V08H), A/Memphis/1/1968 (Cat# 40101-V08H1), A/Vietnam/1194/2004 (Cat# 11062-V08H1), A/Hokkaido/167/2007 (Cat# 11696-V08H), and A/Hong Kong/2009 (Cat# 40174-V08H1) (Sino Biological) in 0.05M Bicarbonate buffer at pH 9.6. .. Coated plates were blocked with 5% BSA in 1X PBS overnight at 4°C, and hu-mAbs (5 μg /ml) were added to the plate and incubated for 2 h at room temperature.

    Clone Assay:

    Article Title: Generation and testing anti-influenza human monoclonal antibodies in a new humanized mouse model (DRAGA: HLA-A2. HLA-DR4. Rag1 KO. IL-2Rγc KO. NOD)
    Article Snippet: Gels were stained with Amido Black for 30 min at room temperature, dried under hot air blower, and de-stained as per manufacturer's instructions. .. ELISA was used to select positive hybridoma clones secreting anti-HA hu-mAbs and to isotype these antibodies, to determine the cross-reactivity of hu-mAbs to rHAs from various influenza virus heterotypes, to measure the BALB/c mouse Ab response to anti-HA hu-mAb treatment and to PR8 virus infection, and to determine the span-life of 16D11 anti-HA hu-mAb in the blood circulation of BALB/c mice; (i) Selection of stable hybridoma clones secreting anti-HA hu-mAbs in the cell culture supernatant and isotyping the affinity-purified hu-mAbs by these clones was carried out by semi-quantitative ELISA kits (Bethyl Laboratories) according to the manufacturer's instructions; (ii) Cross-reactivity of hu-mAbs to rHAs from various influenza virus heterotypes was measured in 96-well plates were coated overnight at 4°C with 20 μg/ml of recombinant HA proteins from A/Puerto Rico/8/1934 (Cat# 11684-V08H), A/WSN/1933 (Cat# 11692-V08H), A/Aichi/2/1968 (Cat# 11707-V08H), A/Memphis/1/1968 (Cat# 40101-V08H1), A/Vietnam/1194/2004 (Cat# 11062-V08H1), A/Hokkaido/167/2007 (Cat# 11696-V08H), and A/Hong Kong/2009 (Cat# 40174-V08H1) (Sino Biological) in 0.05M Bicarbonate buffer at pH 9.6. .. Coated plates were blocked with 5% BSA in 1X PBS overnight at 4°C, and hu-mAbs (5 μg /ml) were added to the plate and incubated for 2 h at room temperature.

    Infection:

    Article Title: Generation and testing anti-influenza human monoclonal antibodies in a new humanized mouse model (DRAGA: HLA-A2. HLA-DR4. Rag1 KO. IL-2Rγc KO. NOD)
    Article Snippet: Gels were stained with Amido Black for 30 min at room temperature, dried under hot air blower, and de-stained as per manufacturer's instructions. .. ELISA was used to select positive hybridoma clones secreting anti-HA hu-mAbs and to isotype these antibodies, to determine the cross-reactivity of hu-mAbs to rHAs from various influenza virus heterotypes, to measure the BALB/c mouse Ab response to anti-HA hu-mAb treatment and to PR8 virus infection, and to determine the span-life of 16D11 anti-HA hu-mAb in the blood circulation of BALB/c mice; (i) Selection of stable hybridoma clones secreting anti-HA hu-mAbs in the cell culture supernatant and isotyping the affinity-purified hu-mAbs by these clones was carried out by semi-quantitative ELISA kits (Bethyl Laboratories) according to the manufacturer's instructions; (ii) Cross-reactivity of hu-mAbs to rHAs from various influenza virus heterotypes was measured in 96-well plates were coated overnight at 4°C with 20 μg/ml of recombinant HA proteins from A/Puerto Rico/8/1934 (Cat# 11684-V08H), A/WSN/1933 (Cat# 11692-V08H), A/Aichi/2/1968 (Cat# 11707-V08H), A/Memphis/1/1968 (Cat# 40101-V08H1), A/Vietnam/1194/2004 (Cat# 11062-V08H1), A/Hokkaido/167/2007 (Cat# 11696-V08H), and A/Hong Kong/2009 (Cat# 40174-V08H1) (Sino Biological) in 0.05M Bicarbonate buffer at pH 9.6. .. Coated plates were blocked with 5% BSA in 1X PBS overnight at 4°C, and hu-mAbs (5 μg /ml) were added to the plate and incubated for 2 h at room temperature.

    Mouse Assay:

    Article Title: Generation and testing anti-influenza human monoclonal antibodies in a new humanized mouse model (DRAGA: HLA-A2. HLA-DR4. Rag1 KO. IL-2Rγc KO. NOD)
    Article Snippet: Gels were stained with Amido Black for 30 min at room temperature, dried under hot air blower, and de-stained as per manufacturer's instructions. .. ELISA was used to select positive hybridoma clones secreting anti-HA hu-mAbs and to isotype these antibodies, to determine the cross-reactivity of hu-mAbs to rHAs from various influenza virus heterotypes, to measure the BALB/c mouse Ab response to anti-HA hu-mAb treatment and to PR8 virus infection, and to determine the span-life of 16D11 anti-HA hu-mAb in the blood circulation of BALB/c mice; (i) Selection of stable hybridoma clones secreting anti-HA hu-mAbs in the cell culture supernatant and isotyping the affinity-purified hu-mAbs by these clones was carried out by semi-quantitative ELISA kits (Bethyl Laboratories) according to the manufacturer's instructions; (ii) Cross-reactivity of hu-mAbs to rHAs from various influenza virus heterotypes was measured in 96-well plates were coated overnight at 4°C with 20 μg/ml of recombinant HA proteins from A/Puerto Rico/8/1934 (Cat# 11684-V08H), A/WSN/1933 (Cat# 11692-V08H), A/Aichi/2/1968 (Cat# 11707-V08H), A/Memphis/1/1968 (Cat# 40101-V08H1), A/Vietnam/1194/2004 (Cat# 11062-V08H1), A/Hokkaido/167/2007 (Cat# 11696-V08H), and A/Hong Kong/2009 (Cat# 40174-V08H1) (Sino Biological) in 0.05M Bicarbonate buffer at pH 9.6. .. Coated plates were blocked with 5% BSA in 1X PBS overnight at 4°C, and hu-mAbs (5 μg /ml) were added to the plate and incubated for 2 h at room temperature.

    Selection:

    Article Title: Generation and testing anti-influenza human monoclonal antibodies in a new humanized mouse model (DRAGA: HLA-A2. HLA-DR4. Rag1 KO. IL-2Rγc KO. NOD)
    Article Snippet: Gels were stained with Amido Black for 30 min at room temperature, dried under hot air blower, and de-stained as per manufacturer's instructions. .. ELISA was used to select positive hybridoma clones secreting anti-HA hu-mAbs and to isotype these antibodies, to determine the cross-reactivity of hu-mAbs to rHAs from various influenza virus heterotypes, to measure the BALB/c mouse Ab response to anti-HA hu-mAb treatment and to PR8 virus infection, and to determine the span-life of 16D11 anti-HA hu-mAb in the blood circulation of BALB/c mice; (i) Selection of stable hybridoma clones secreting anti-HA hu-mAbs in the cell culture supernatant and isotyping the affinity-purified hu-mAbs by these clones was carried out by semi-quantitative ELISA kits (Bethyl Laboratories) according to the manufacturer's instructions; (ii) Cross-reactivity of hu-mAbs to rHAs from various influenza virus heterotypes was measured in 96-well plates were coated overnight at 4°C with 20 μg/ml of recombinant HA proteins from A/Puerto Rico/8/1934 (Cat# 11684-V08H), A/WSN/1933 (Cat# 11692-V08H), A/Aichi/2/1968 (Cat# 11707-V08H), A/Memphis/1/1968 (Cat# 40101-V08H1), A/Vietnam/1194/2004 (Cat# 11062-V08H1), A/Hokkaido/167/2007 (Cat# 11696-V08H), and A/Hong Kong/2009 (Cat# 40174-V08H1) (Sino Biological) in 0.05M Bicarbonate buffer at pH 9.6. .. Coated plates were blocked with 5% BSA in 1X PBS overnight at 4°C, and hu-mAbs (5 μg /ml) were added to the plate and incubated for 2 h at room temperature.

    Cell Culture:

    Article Title: Generation and testing anti-influenza human monoclonal antibodies in a new humanized mouse model (DRAGA: HLA-A2. HLA-DR4. Rag1 KO. IL-2Rγc KO. NOD)
    Article Snippet: Gels were stained with Amido Black for 30 min at room temperature, dried under hot air blower, and de-stained as per manufacturer's instructions. .. ELISA was used to select positive hybridoma clones secreting anti-HA hu-mAbs and to isotype these antibodies, to determine the cross-reactivity of hu-mAbs to rHAs from various influenza virus heterotypes, to measure the BALB/c mouse Ab response to anti-HA hu-mAb treatment and to PR8 virus infection, and to determine the span-life of 16D11 anti-HA hu-mAb in the blood circulation of BALB/c mice; (i) Selection of stable hybridoma clones secreting anti-HA hu-mAbs in the cell culture supernatant and isotyping the affinity-purified hu-mAbs by these clones was carried out by semi-quantitative ELISA kits (Bethyl Laboratories) according to the manufacturer's instructions; (ii) Cross-reactivity of hu-mAbs to rHAs from various influenza virus heterotypes was measured in 96-well plates were coated overnight at 4°C with 20 μg/ml of recombinant HA proteins from A/Puerto Rico/8/1934 (Cat# 11684-V08H), A/WSN/1933 (Cat# 11692-V08H), A/Aichi/2/1968 (Cat# 11707-V08H), A/Memphis/1/1968 (Cat# 40101-V08H1), A/Vietnam/1194/2004 (Cat# 11062-V08H1), A/Hokkaido/167/2007 (Cat# 11696-V08H), and A/Hong Kong/2009 (Cat# 40174-V08H1) (Sino Biological) in 0.05M Bicarbonate buffer at pH 9.6. .. Coated plates were blocked with 5% BSA in 1X PBS overnight at 4°C, and hu-mAbs (5 μg /ml) were added to the plate and incubated for 2 h at room temperature.

    Affinity Purification:

    Article Title: Generation and testing anti-influenza human monoclonal antibodies in a new humanized mouse model (DRAGA: HLA-A2. HLA-DR4. Rag1 KO. IL-2Rγc KO. NOD)
    Article Snippet: Gels were stained with Amido Black for 30 min at room temperature, dried under hot air blower, and de-stained as per manufacturer's instructions. .. ELISA was used to select positive hybridoma clones secreting anti-HA hu-mAbs and to isotype these antibodies, to determine the cross-reactivity of hu-mAbs to rHAs from various influenza virus heterotypes, to measure the BALB/c mouse Ab response to anti-HA hu-mAb treatment and to PR8 virus infection, and to determine the span-life of 16D11 anti-HA hu-mAb in the blood circulation of BALB/c mice; (i) Selection of stable hybridoma clones secreting anti-HA hu-mAbs in the cell culture supernatant and isotyping the affinity-purified hu-mAbs by these clones was carried out by semi-quantitative ELISA kits (Bethyl Laboratories) according to the manufacturer's instructions; (ii) Cross-reactivity of hu-mAbs to rHAs from various influenza virus heterotypes was measured in 96-well plates were coated overnight at 4°C with 20 μg/ml of recombinant HA proteins from A/Puerto Rico/8/1934 (Cat# 11684-V08H), A/WSN/1933 (Cat# 11692-V08H), A/Aichi/2/1968 (Cat# 11707-V08H), A/Memphis/1/1968 (Cat# 40101-V08H1), A/Vietnam/1194/2004 (Cat# 11062-V08H1), A/Hokkaido/167/2007 (Cat# 11696-V08H), and A/Hong Kong/2009 (Cat# 40174-V08H1) (Sino Biological) in 0.05M Bicarbonate buffer at pH 9.6. .. Coated plates were blocked with 5% BSA in 1X PBS overnight at 4°C, and hu-mAbs (5 μg /ml) were added to the plate and incubated for 2 h at room temperature.

    Recombinant:

    Article Title: Generation and testing anti-influenza human monoclonal antibodies in a new humanized mouse model (DRAGA: HLA-A2. HLA-DR4. Rag1 KO. IL-2Rγc KO. NOD)
    Article Snippet: Gels were stained with Amido Black for 30 min at room temperature, dried under hot air blower, and de-stained as per manufacturer's instructions. .. ELISA was used to select positive hybridoma clones secreting anti-HA hu-mAbs and to isotype these antibodies, to determine the cross-reactivity of hu-mAbs to rHAs from various influenza virus heterotypes, to measure the BALB/c mouse Ab response to anti-HA hu-mAb treatment and to PR8 virus infection, and to determine the span-life of 16D11 anti-HA hu-mAb in the blood circulation of BALB/c mice; (i) Selection of stable hybridoma clones secreting anti-HA hu-mAbs in the cell culture supernatant and isotyping the affinity-purified hu-mAbs by these clones was carried out by semi-quantitative ELISA kits (Bethyl Laboratories) according to the manufacturer's instructions; (ii) Cross-reactivity of hu-mAbs to rHAs from various influenza virus heterotypes was measured in 96-well plates were coated overnight at 4°C with 20 μg/ml of recombinant HA proteins from A/Puerto Rico/8/1934 (Cat# 11684-V08H), A/WSN/1933 (Cat# 11692-V08H), A/Aichi/2/1968 (Cat# 11707-V08H), A/Memphis/1/1968 (Cat# 40101-V08H1), A/Vietnam/1194/2004 (Cat# 11062-V08H1), A/Hokkaido/167/2007 (Cat# 11696-V08H), and A/Hong Kong/2009 (Cat# 40174-V08H1) (Sino Biological) in 0.05M Bicarbonate buffer at pH 9.6. .. Coated plates were blocked with 5% BSA in 1X PBS overnight at 4°C, and hu-mAbs (5 μg /ml) were added to the plate and incubated for 2 h at room temperature.

    Article Title: A DNA Vaccine That Targets Hemagglutinin to Antigen-Presenting Cells Protects Mice against H7 Influenza
    Article Snippet: H7 GFP cells and mCherry cells (5 × 106; 1:1) were injected intravenously (i.v.) into BALB/c mice, and the prevalence of GFP-positive and mCherry-positive cells in the spleen were analyzed 16 h later in an Attune NxT flow cytometer (Thermo Fisher Scientific, Waltham, MA) using FlowJo software. .. Next, 0.5 × 106 cells were seeded per well, and cells were stimulated with either medium (negative control), ConA (1 μg/ml, positive control), or 10 μg/ml recombinant HA from either A/Shanghai/1/2013 (H7N9), or A/Puerto Rico/8/1934 (H1N1), or A/Vietnam/1194/2004 (H5N1) (40104-V08B, 11684-V08H, and 11062-V08H1, respectively; Sino Biological). .. Plates were incubated for 20 h at 37°C in 5% CO2 before incubation with the detection antibody (3321-4APT; Mabtech) and development with the BCIP/NBT-Purple liquid substrate system for membranes (B3679; Sigma-Aldrich).

    Negative Control:

    Article Title: A DNA Vaccine That Targets Hemagglutinin to Antigen-Presenting Cells Protects Mice against H7 Influenza
    Article Snippet: H7 GFP cells and mCherry cells (5 × 106; 1:1) were injected intravenously (i.v.) into BALB/c mice, and the prevalence of GFP-positive and mCherry-positive cells in the spleen were analyzed 16 h later in an Attune NxT flow cytometer (Thermo Fisher Scientific, Waltham, MA) using FlowJo software. .. Next, 0.5 × 106 cells were seeded per well, and cells were stimulated with either medium (negative control), ConA (1 μg/ml, positive control), or 10 μg/ml recombinant HA from either A/Shanghai/1/2013 (H7N9), or A/Puerto Rico/8/1934 (H1N1), or A/Vietnam/1194/2004 (H5N1) (40104-V08B, 11684-V08H, and 11062-V08H1, respectively; Sino Biological). .. Plates were incubated for 20 h at 37°C in 5% CO2 before incubation with the detection antibody (3321-4APT; Mabtech) and development with the BCIP/NBT-Purple liquid substrate system for membranes (B3679; Sigma-Aldrich).

    Positive Control:

    Article Title: A DNA Vaccine That Targets Hemagglutinin to Antigen-Presenting Cells Protects Mice against H7 Influenza
    Article Snippet: H7 GFP cells and mCherry cells (5 × 106; 1:1) were injected intravenously (i.v.) into BALB/c mice, and the prevalence of GFP-positive and mCherry-positive cells in the spleen were analyzed 16 h later in an Attune NxT flow cytometer (Thermo Fisher Scientific, Waltham, MA) using FlowJo software. .. Next, 0.5 × 106 cells were seeded per well, and cells were stimulated with either medium (negative control), ConA (1 μg/ml, positive control), or 10 μg/ml recombinant HA from either A/Shanghai/1/2013 (H7N9), or A/Puerto Rico/8/1934 (H1N1), or A/Vietnam/1194/2004 (H5N1) (40104-V08B, 11684-V08H, and 11062-V08H1, respectively; Sino Biological). .. Plates were incubated for 20 h at 37°C in 5% CO2 before incubation with the detection antibody (3321-4APT; Mabtech) and development with the BCIP/NBT-Purple liquid substrate system for membranes (B3679; Sigma-Aldrich).

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    Sino Biological influenza a h5n1 hemagglutinin ha antibody rabbit pab
    MAb-mediated protection against a lethal challenge of <t>H5N1</t> virus in mice
    Influenza A H5n1 Hemagglutinin Ha Antibody Rabbit Pab, supplied by Sino Biological, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/influenza a h5n1 hemagglutinin ha antibody rabbit pab/product/Sino Biological
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    influenza a h5n1 hemagglutinin ha antibody rabbit pab - by Bioz Stars, 2021-06
    88/100 stars
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    94
    Sino Biological influenza a h5n1 ha protein
    Competition-mediated detection of test epitope presentation on MHC-II. a F24 staining of 1055 HLA-DRB1*15:01 and 9036 *11:01 pulsed with p24 (reference epitope) alone or with an increasing concentration of HA test antigen (test peptide). b F11 staining of 1111 HLA-DRB1*04:01 and 1124 *01:01 cells pulsed with HA (reference epitope) alone or with an increasing concentration p24 test antigen (test peptide) ( c , d ). F24 staining of 9036 HLA-DRB1*11:01 and 1055 *15:01 cells was pulsed with p24 alone or in the presence of Omomyc ( c ) or <t>influenza</t> A <t>H5N1</t> HA protein ( d ). The data on Y -axis is expressed as the percent sTCR florescence signal (MFI) relative to cells pulsed with reference epitope: MFI (test compound + reference epitope) / median MFI (reference epitope alone). The one-way ANOVA with multiple comparisons was used to determine significance, p > 0.05 not significant ( ns ). For statistical comparisons in a and b , samples treated with test peptide were compared to no test epitope; for c and d , samples treated with reference peptide + protein were compared to the reference epitope alone
    Influenza A H5n1 Ha Protein, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/influenza a h5n1 ha protein/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    influenza a h5n1 ha protein - by Bioz Stars, 2021-06
    94/100 stars
      Buy from Supplier

    85
    Sino Biological influenza h5n1 a vietnam 1194 2004 hemagglutinin ha antibody rabbit pab
    Competition-mediated detection of test epitope presentation on MHC-II. a F24 staining of 1055 HLA-DRB1*15:01 and 9036 *11:01 pulsed with p24 (reference epitope) alone or with an increasing concentration of HA test antigen (test peptide). b F11 staining of 1111 HLA-DRB1*04:01 and 1124 *01:01 cells pulsed with HA (reference epitope) alone or with an increasing concentration p24 test antigen (test peptide) ( c , d ). F24 staining of 9036 HLA-DRB1*11:01 and 1055 *15:01 cells was pulsed with p24 alone or in the presence of Omomyc ( c ) or <t>influenza</t> A <t>H5N1</t> HA protein ( d ). The data on Y -axis is expressed as the percent sTCR florescence signal (MFI) relative to cells pulsed with reference epitope: MFI (test compound + reference epitope) / median MFI (reference epitope alone). The one-way ANOVA with multiple comparisons was used to determine significance, p > 0.05 not significant ( ns ). For statistical comparisons in a and b , samples treated with test peptide were compared to no test epitope; for c and d , samples treated with reference peptide + protein were compared to the reference epitope alone
    Influenza H5n1 A Vietnam 1194 2004 Hemagglutinin Ha Antibody Rabbit Pab, supplied by Sino Biological, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/influenza h5n1 a vietnam 1194 2004 hemagglutinin ha antibody rabbit pab/product/Sino Biological
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    influenza h5n1 a vietnam 1194 2004 hemagglutinin ha antibody rabbit pab - by Bioz Stars, 2021-06
    85/100 stars
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    MAb-mediated protection against a lethal challenge of H5N1 virus in mice

    Journal: Antiviral research

    Article Title: Selection of Therapeutic H5N1 Monoclonal Antibodies Following IgVH Repertoire Analysis in Mice

    doi: 10.1016/j.antiviral.2016.04.001

    Figure Lengend Snippet: MAb-mediated protection against a lethal challenge of H5N1 virus in mice

    Article Snippet: Hemagglutination inhibition (HI) activity specific to A/Vietnam/1194/2004 (H5N1), A/Cambodia/RO405050/2007 (H5N1), A/turkey/Turkey/1/2005 (H5N1), and A/H5N1/Anhui/1/05 (H5N1) virus (NIBSC, Hertfordshire England) were determined ( ).

    Techniques: Mouse Assay

    Competition-mediated detection of test epitope presentation on MHC-II. a F24 staining of 1055 HLA-DRB1*15:01 and 9036 *11:01 pulsed with p24 (reference epitope) alone or with an increasing concentration of HA test antigen (test peptide). b F11 staining of 1111 HLA-DRB1*04:01 and 1124 *01:01 cells pulsed with HA (reference epitope) alone or with an increasing concentration p24 test antigen (test peptide) ( c , d ). F24 staining of 9036 HLA-DRB1*11:01 and 1055 *15:01 cells was pulsed with p24 alone or in the presence of Omomyc ( c ) or influenza A H5N1 HA protein ( d ). The data on Y -axis is expressed as the percent sTCR florescence signal (MFI) relative to cells pulsed with reference epitope: MFI (test compound + reference epitope) / median MFI (reference epitope alone). The one-way ANOVA with multiple comparisons was used to determine significance, p > 0.05 not significant ( ns ). For statistical comparisons in a and b , samples treated with test peptide were compared to no test epitope; for c and d , samples treated with reference peptide + protein were compared to the reference epitope alone

    Journal: The AAPS Journal

    Article Title: Competition-Based Cell Assay Employing Soluble T Cell Receptors to Assess MHC Class II Antigen Processing and Presentation

    doi: 10.1208/s12248-020-00553-x

    Figure Lengend Snippet: Competition-mediated detection of test epitope presentation on MHC-II. a F24 staining of 1055 HLA-DRB1*15:01 and 9036 *11:01 pulsed with p24 (reference epitope) alone or with an increasing concentration of HA test antigen (test peptide). b F11 staining of 1111 HLA-DRB1*04:01 and 1124 *01:01 cells pulsed with HA (reference epitope) alone or with an increasing concentration p24 test antigen (test peptide) ( c , d ). F24 staining of 9036 HLA-DRB1*11:01 and 1055 *15:01 cells was pulsed with p24 alone or in the presence of Omomyc ( c ) or influenza A H5N1 HA protein ( d ). The data on Y -axis is expressed as the percent sTCR florescence signal (MFI) relative to cells pulsed with reference epitope: MFI (test compound + reference epitope) / median MFI (reference epitope alone). The one-way ANOVA with multiple comparisons was used to determine significance, p > 0.05 not significant ( ns ). For statistical comparisons in a and b , samples treated with test peptide were compared to no test epitope; for c and d , samples treated with reference peptide + protein were compared to the reference epitope alone

    Article Snippet: Influenza A H5N1 HA protein (A/Vietnam/1194/2004) was purchased from Sino Biological (cat# 11062-V08H1).

    Techniques: Staining, Concentration Assay