h1n1  (Sino Biological)


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    Name:
    Influenza A H1N1 Neuraminidase NA
    Description:
    A DNA sequence encoding the mature form of Influenza A virus H1N1 A California 04 2009 neuraminidase ACP41107 1 His 36 Lys 469 was expressed with the fused Fc region of human IgG1 at the N terminus linked by a peptide linker
    Catalog Number:
    11058-V01H
    Price:
    None
    Category:
    recombinant protein
    Product Aliases:
    NA Protein H1N1
    Host:
    HEK293 Cells
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    Structured Review

    Sino Biological h1n1
    Influenza A <t>H1N1</t> virus T-cell epitope screen in HLA-DQ6.2 mice, and Pandemrix-vaccinated HLA-DQB1*0602 positive individuals. Spleen cells from Pandemrix-immunized HLA-DQ6.2 mice were stimulated in culture with pools of five overlapping 15-mer peptides each, covering a hemagglutinin (HA), b neuraminidase (NA) or c nucleoprotein (NP) from influenza (A/reassortant/NYMC X-179A (California/07/2009 × NYMC X-157)(H1N1)) vaccine virus used in Pandemrix (pooling cells from 2 × 2 mice, n = 2). Recombinant hemagglutinin (rHA) and nucleoprotein (rNP; A/Puerto Rico/8/34 corresponding to the Pandemrix vaccine strain) were used as positive controls. d – f PBMC from Pandemrix-vaccinated HLA-DQB1*0602 positive individuals (sleep clinic patients without a diagnosis of NT1) were stimulated in culture with single 15-mer peptides, derived from the same vaccine virus strain ( n = 1–2). The expression of IFN-γ or IL-2 was measured by FMIA (protein) or RT-qPCR (mRNA). Results are expressed as ratios between cytokine concentrations (lines representing means) or relative gene expressions (dots representing single values, bars representing means) measured in peptide-stimulated and negative control samples (stimulation index). An asterisk (*) indicates a pool or single peptide that was selected for further testing.
    A DNA sequence encoding the mature form of Influenza A virus H1N1 A California 04 2009 neuraminidase ACP41107 1 His 36 Lys 469 was expressed with the fused Fc region of human IgG1 at the N terminus linked by a peptide linker
    https://www.bioz.com/result/h1n1/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    h1n1 - by Bioz Stars, 2021-06
    94/100 stars

    Images

    1) Product Images from "Enhanced influenza A H1N1 T cell epitope recognition and cross-reactivity to protein-O-mannosyltransferase 1 in Pandemrix-associated narcolepsy type 1"

    Article Title: Enhanced influenza A H1N1 T cell epitope recognition and cross-reactivity to protein-O-mannosyltransferase 1 in Pandemrix-associated narcolepsy type 1

    Journal: Nature Communications

    doi: 10.1038/s41467-021-22637-8

    Influenza A H1N1 virus T-cell epitope screen in HLA-DQ6.2 mice, and Pandemrix-vaccinated HLA-DQB1*0602 positive individuals. Spleen cells from Pandemrix-immunized HLA-DQ6.2 mice were stimulated in culture with pools of five overlapping 15-mer peptides each, covering a hemagglutinin (HA), b neuraminidase (NA) or c nucleoprotein (NP) from influenza (A/reassortant/NYMC X-179A (California/07/2009 × NYMC X-157)(H1N1)) vaccine virus used in Pandemrix (pooling cells from 2 × 2 mice, n = 2). Recombinant hemagglutinin (rHA) and nucleoprotein (rNP; A/Puerto Rico/8/34 corresponding to the Pandemrix vaccine strain) were used as positive controls. d – f PBMC from Pandemrix-vaccinated HLA-DQB1*0602 positive individuals (sleep clinic patients without a diagnosis of NT1) were stimulated in culture with single 15-mer peptides, derived from the same vaccine virus strain ( n = 1–2). The expression of IFN-γ or IL-2 was measured by FMIA (protein) or RT-qPCR (mRNA). Results are expressed as ratios between cytokine concentrations (lines representing means) or relative gene expressions (dots representing single values, bars representing means) measured in peptide-stimulated and negative control samples (stimulation index). An asterisk (*) indicates a pool or single peptide that was selected for further testing.
    Figure Legend Snippet: Influenza A H1N1 virus T-cell epitope screen in HLA-DQ6.2 mice, and Pandemrix-vaccinated HLA-DQB1*0602 positive individuals. Spleen cells from Pandemrix-immunized HLA-DQ6.2 mice were stimulated in culture with pools of five overlapping 15-mer peptides each, covering a hemagglutinin (HA), b neuraminidase (NA) or c nucleoprotein (NP) from influenza (A/reassortant/NYMC X-179A (California/07/2009 × NYMC X-157)(H1N1)) vaccine virus used in Pandemrix (pooling cells from 2 × 2 mice, n = 2). Recombinant hemagglutinin (rHA) and nucleoprotein (rNP; A/Puerto Rico/8/34 corresponding to the Pandemrix vaccine strain) were used as positive controls. d – f PBMC from Pandemrix-vaccinated HLA-DQB1*0602 positive individuals (sleep clinic patients without a diagnosis of NT1) were stimulated in culture with single 15-mer peptides, derived from the same vaccine virus strain ( n = 1–2). The expression of IFN-γ or IL-2 was measured by FMIA (protein) or RT-qPCR (mRNA). Results are expressed as ratios between cytokine concentrations (lines representing means) or relative gene expressions (dots representing single values, bars representing means) measured in peptide-stimulated and negative control samples (stimulation index). An asterisk (*) indicates a pool or single peptide that was selected for further testing.

    Techniques Used: Mouse Assay, Recombinant, Derivative Assay, Expressing, Quantitative RT-PCR, Negative Control

    Mapping of identified influenza A H1N1 virus T-cell epitopes in Pandemrix-associated NT1 patients. a , d PBMC from pediatric Pandemrix-associated NT1 patients (NT1; validation cohort) or pediatric Pandemrix-vaccinated healthy controls (C) were stimulated in culture with overlapping 15-mer peptides from influenza (A/reassortant/NYMC X-179A (California/07/2009 × NYMC X-157)(H1N1)) vaccine virus neuraminidase (NA) or nucleoprotein (NP), as indicated. b , c , e , f PBMC from NT1 patients (invariably HLA-DQB1*0602 positive; discovery and validation cohorts combined; HLA-DQB1*0602 homozygous NT1 patients marked with red dots) or HLA-DQB1*0602 positive (C/DQ6+) or negative (C/DQ6−) healthy controls were stimulated with single NA- or NP-derived peptides. The secretion of IFN-γ was measured by FMIA (protein). Results are expressed as the ratio between cytokine concentrations measured in peptide-stimulated and negative control samples (stimulation index). Statistical comparisons between groups were performed, using Kruskal–Wallis and Dunn’s multiple comparisons tests.
    Figure Legend Snippet: Mapping of identified influenza A H1N1 virus T-cell epitopes in Pandemrix-associated NT1 patients. a , d PBMC from pediatric Pandemrix-associated NT1 patients (NT1; validation cohort) or pediatric Pandemrix-vaccinated healthy controls (C) were stimulated in culture with overlapping 15-mer peptides from influenza (A/reassortant/NYMC X-179A (California/07/2009 × NYMC X-157)(H1N1)) vaccine virus neuraminidase (NA) or nucleoprotein (NP), as indicated. b , c , e , f PBMC from NT1 patients (invariably HLA-DQB1*0602 positive; discovery and validation cohorts combined; HLA-DQB1*0602 homozygous NT1 patients marked with red dots) or HLA-DQB1*0602 positive (C/DQ6+) or negative (C/DQ6−) healthy controls were stimulated with single NA- or NP-derived peptides. The secretion of IFN-γ was measured by FMIA (protein). Results are expressed as the ratio between cytokine concentrations measured in peptide-stimulated and negative control samples (stimulation index). Statistical comparisons between groups were performed, using Kruskal–Wallis and Dunn’s multiple comparisons tests.

    Techniques Used: Derivative Assay, Negative Control

    Identification of influenza A H1N1 virus T-cell epitopes in Pandemrix-associated NT1 patients. PBMC from pediatric Pandemrix-associated NT1 patients (NT1) or pediatric Pandemrix-vaccinated healthy controls (C) were stimulated in culture with single 15-mer peptides from influenza (A/reassortant/NYMC X-179A (California/07/2009 × NYMC X-157)(H1N1)) vaccine virus hemagglutinin (HA), neuraminidase (NA) or nucleoprotein (NP) (discovery cohort). Recombinant neuraminidase (rNA) and nucleoprotein (rNP) were used as positive controls. The secretion of IFN-γ ( a , c , e ) or IL-2 ( b , d , f ) was measured by FMIA (protein). Results are expressed as the ratio between cytokine concentrations measured in peptide-stimulated and negative control samples (stimulation index). Statistical comparisons between groups were performed, using Kruskal–Wallis and Dunn’s multiple comparisons tests.
    Figure Legend Snippet: Identification of influenza A H1N1 virus T-cell epitopes in Pandemrix-associated NT1 patients. PBMC from pediatric Pandemrix-associated NT1 patients (NT1) or pediatric Pandemrix-vaccinated healthy controls (C) were stimulated in culture with single 15-mer peptides from influenza (A/reassortant/NYMC X-179A (California/07/2009 × NYMC X-157)(H1N1)) vaccine virus hemagglutinin (HA), neuraminidase (NA) or nucleoprotein (NP) (discovery cohort). Recombinant neuraminidase (rNA) and nucleoprotein (rNP) were used as positive controls. The secretion of IFN-γ ( a , c , e ) or IL-2 ( b , d , f ) was measured by FMIA (protein). Results are expressed as the ratio between cytokine concentrations measured in peptide-stimulated and negative control samples (stimulation index). Statistical comparisons between groups were performed, using Kruskal–Wallis and Dunn’s multiple comparisons tests.

    Techniques Used: Recombinant, Negative Control

    Related Articles

    Activity Assay:

    Article Title: Self-enzyme chemiluminescence immunoassay capable of rapidly diagnosing the infection of influenza A (H1N1) virus.
    Article Snippet: .. A highly sensitive self-enzyme immunoassay with 1,1'-oxalyldiimidazole chemiluminescence (ODI-CL) detection was developed for the first time to quantify influenza A (H1N1) virus. .. A highly sensitive self-enzyme immunoassay with 1,1'-oxalyldiimidazole chemiluminescence (ODI-CL) detection was developed for the first time to quantify influenza A (H1N1) virus.

    Derivative Assay:

    Article Title: Pan-Influenza A Protection by Prime–Boost Vaccination with Cold-Adapted Live-Attenuated Influenza Vaccine in a Mouse Model
    Article Snippet: Recombinant Influenza HA Proteins The HA proteins expressed in insect cells were purchased from Sino Biological (China). .. The seven different HA proteins were derived from A/California/6/2009 (H1N1), A/Puerto Rico/8/1934 (H1N1), A/Canada/720/2006 (H2N2), A/Indonesia/5/2005 (H5N1), A/Hong Kong/35820/2009 (H9N2), A/Sydney/5/1997 (H3N2), and A/Anhui/1/2013 (H7N9) influenza viruses. .. We also expressed the HA proteins using bacterial expression system.

    Article Title: Synthesis and anti-influenza virus activity of 4-oxo- or thioxo-4,5-dihydrofuro[3,4-c]pyridin-3(1H)-ones
    Article Snippet: 2.10 NA assay NA assay was performed by using a NA-XTD Influenza Neuraminidase Assay kit (Applied Biosystems, Foster City, CA) according to the manufacturer’s protocol with some modifications ( ). .. In brief, 25 μl of influenza A virus PR8 [1 × 105 plaque-forming units (PFU)/ml] or purified NA proteins derived from influenza H1N1 (A/California/04/2009) and its OSV-resistant H275Y mutant [NA (H275Y)] (Sino Biological) [25 μl of 40 milliunits (mU) per ml] in PBS were mixed individually with an equal volume of serially diluted test compounds in 96-well plates and incubated at 37 °C for 20 min. After adding 25 μl of 5 μM NA-XTD substrate (Applied Biosystems), the reaction mixture was incubated at RT for 20 min. .. The luminescent signal was maximized by the addition of 60 μl of NA-XTD accelerator and measured with a Berthold LB960 Centro microplate luminometer (Berthold Technologies, Bad Wilbad, Germany) using a 1 s/well read time.

    Purification:

    Article Title: Synthesis and anti-influenza virus activity of 4-oxo- or thioxo-4,5-dihydrofuro[3,4-c]pyridin-3(1H)-ones
    Article Snippet: 2.10 NA assay NA assay was performed by using a NA-XTD Influenza Neuraminidase Assay kit (Applied Biosystems, Foster City, CA) according to the manufacturer’s protocol with some modifications ( ). .. In brief, 25 μl of influenza A virus PR8 [1 × 105 plaque-forming units (PFU)/ml] or purified NA proteins derived from influenza H1N1 (A/California/04/2009) and its OSV-resistant H275Y mutant [NA (H275Y)] (Sino Biological) [25 μl of 40 milliunits (mU) per ml] in PBS were mixed individually with an equal volume of serially diluted test compounds in 96-well plates and incubated at 37 °C for 20 min. After adding 25 μl of 5 μM NA-XTD substrate (Applied Biosystems), the reaction mixture was incubated at RT for 20 min. .. The luminescent signal was maximized by the addition of 60 μl of NA-XTD accelerator and measured with a Berthold LB960 Centro microplate luminometer (Berthold Technologies, Bad Wilbad, Germany) using a 1 s/well read time.

    Article Title: Development of a Point-of-Care Diagnostic for Influenza Detection with Antiviral Treatment Effectiveness Indication
    Article Snippet: The 5-Bromo-4-chloro-3-indoyl-α-D-N-acetylneuraminic acid (X-NeuNAc) was obtained from Santa Cruz Biosciences. .. Purified influenza A H1N1 (A/California/07/09 H1N1 or A(H1N1)pdm09) Neuraminidase protein was obtained from Sino Biological. .. Pullulan from Aureobasidium pullulans was obtained from Sigma Aldrich.

    Mutagenesis:

    Article Title: Synthesis and anti-influenza virus activity of 4-oxo- or thioxo-4,5-dihydrofuro[3,4-c]pyridin-3(1H)-ones
    Article Snippet: 2.10 NA assay NA assay was performed by using a NA-XTD Influenza Neuraminidase Assay kit (Applied Biosystems, Foster City, CA) according to the manufacturer’s protocol with some modifications ( ). .. In brief, 25 μl of influenza A virus PR8 [1 × 105 plaque-forming units (PFU)/ml] or purified NA proteins derived from influenza H1N1 (A/California/04/2009) and its OSV-resistant H275Y mutant [NA (H275Y)] (Sino Biological) [25 μl of 40 milliunits (mU) per ml] in PBS were mixed individually with an equal volume of serially diluted test compounds in 96-well plates and incubated at 37 °C for 20 min. After adding 25 μl of 5 μM NA-XTD substrate (Applied Biosystems), the reaction mixture was incubated at RT for 20 min. .. The luminescent signal was maximized by the addition of 60 μl of NA-XTD accelerator and measured with a Berthold LB960 Centro microplate luminometer (Berthold Technologies, Bad Wilbad, Germany) using a 1 s/well read time.

    Incubation:

    Article Title: Synthesis and anti-influenza virus activity of 4-oxo- or thioxo-4,5-dihydrofuro[3,4-c]pyridin-3(1H)-ones
    Article Snippet: 2.10 NA assay NA assay was performed by using a NA-XTD Influenza Neuraminidase Assay kit (Applied Biosystems, Foster City, CA) according to the manufacturer’s protocol with some modifications ( ). .. In brief, 25 μl of influenza A virus PR8 [1 × 105 plaque-forming units (PFU)/ml] or purified NA proteins derived from influenza H1N1 (A/California/04/2009) and its OSV-resistant H275Y mutant [NA (H275Y)] (Sino Biological) [25 μl of 40 milliunits (mU) per ml] in PBS were mixed individually with an equal volume of serially diluted test compounds in 96-well plates and incubated at 37 °C for 20 min. After adding 25 μl of 5 μM NA-XTD substrate (Applied Biosystems), the reaction mixture was incubated at RT for 20 min. .. The luminescent signal was maximized by the addition of 60 μl of NA-XTD accelerator and measured with a Berthold LB960 Centro microplate luminometer (Berthold Technologies, Bad Wilbad, Germany) using a 1 s/well read time.

    Recombinant:

    Article Title: Enhanced influenza A H1N1 T cell epitope recognition and cross-reactivity to protein-O-mannosyltransferase 1 in Pandemrix-associated narcolepsy type 1
    Article Snippet: Peptides were dissolved in endotoxin-free water/DMSO (50%; both from Sigma-Aldrich, Schnelldorf, Germany), and stored as 25 mM stock solutions at −70 °C, until the day of use. .. Recombinant influenza A H1N1 (A/California/07/2009) hemagglutinin (cat#11085-V08H), (A/California/04/2009) neuraminidase (cat#11058-VNAHC or cat#11058-V01H), and (A/California/07/2009) or (A/Puerto Rico/8/34/Mount Sinai) nucleoprotein (cat#40205-V08B and cat#11675-V08B) were purchased from Sino Biological, Beijing, China. .. Endotoxin-free, recombinant human protein-O-mannosyltransferase 1 and syntrophin gamma 1 were from Origene, Rockville, Maryland, and endotoxin-free ovalbumin from Hyglos, Bernried, Germany.

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  • 94
    Sino Biological h1n1
    Influenza A <t>H1N1</t> virus T-cell epitope screen in HLA-DQ6.2 mice, and Pandemrix-vaccinated HLA-DQB1*0602 positive individuals. Spleen cells from Pandemrix-immunized HLA-DQ6.2 mice were stimulated in culture with pools of five overlapping 15-mer peptides each, covering a hemagglutinin (HA), b neuraminidase (NA) or c nucleoprotein (NP) from influenza (A/reassortant/NYMC X-179A (California/07/2009 × NYMC X-157)(H1N1)) vaccine virus used in Pandemrix (pooling cells from 2 × 2 mice, n = 2). Recombinant hemagglutinin (rHA) and nucleoprotein (rNP; A/Puerto Rico/8/34 corresponding to the Pandemrix vaccine strain) were used as positive controls. d – f PBMC from Pandemrix-vaccinated HLA-DQB1*0602 positive individuals (sleep clinic patients without a diagnosis of NT1) were stimulated in culture with single 15-mer peptides, derived from the same vaccine virus strain ( n = 1–2). The expression of IFN-γ or IL-2 was measured by FMIA (protein) or RT-qPCR (mRNA). Results are expressed as ratios between cytokine concentrations (lines representing means) or relative gene expressions (dots representing single values, bars representing means) measured in peptide-stimulated and negative control samples (stimulation index). An asterisk (*) indicates a pool or single peptide that was selected for further testing.
    H1n1, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/h1n1/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    h1n1 - by Bioz Stars, 2021-06
    94/100 stars
      Buy from Supplier

    90
    Sino Biological influenza a h1n1 neuraminidase na
    Influenza A <t>H1N1</t> virus T-cell epitope screen in HLA-DQ6.2 mice, and Pandemrix-vaccinated HLA-DQB1*0602 positive individuals. Spleen cells from Pandemrix-immunized HLA-DQ6.2 mice were stimulated in culture with pools of five overlapping 15-mer peptides each, covering a hemagglutinin (HA), b neuraminidase (NA) or c nucleoprotein (NP) from influenza (A/reassortant/NYMC X-179A (California/07/2009 × NYMC X-157)(H1N1)) vaccine virus used in Pandemrix (pooling cells from 2 × 2 mice, n = 2). Recombinant hemagglutinin (rHA) and nucleoprotein (rNP; A/Puerto Rico/8/34 corresponding to the Pandemrix vaccine strain) were used as positive controls. d – f PBMC from Pandemrix-vaccinated HLA-DQB1*0602 positive individuals (sleep clinic patients without a diagnosis of NT1) were stimulated in culture with single 15-mer peptides, derived from the same vaccine virus strain ( n = 1–2). The expression of IFN-γ or IL-2 was measured by FMIA (protein) or RT-qPCR (mRNA). Results are expressed as ratios between cytokine concentrations (lines representing means) or relative gene expressions (dots representing single values, bars representing means) measured in peptide-stimulated and negative control samples (stimulation index). An asterisk (*) indicates a pool or single peptide that was selected for further testing.
    Influenza A H1n1 Neuraminidase Na, supplied by Sino Biological, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/influenza a h1n1 neuraminidase na/product/Sino Biological
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    influenza a h1n1 neuraminidase na - by Bioz Stars, 2021-06
    90/100 stars
      Buy from Supplier

    92
    Sino Biological anti influenza a h1n1
    Influenza A <t>H1N1</t> virus T-cell epitope screen in HLA-DQ6.2 mice, and Pandemrix-vaccinated HLA-DQB1*0602 positive individuals. Spleen cells from Pandemrix-immunized HLA-DQ6.2 mice were stimulated in culture with pools of five overlapping 15-mer peptides each, covering a hemagglutinin (HA), b neuraminidase (NA) or c nucleoprotein (NP) from influenza (A/reassortant/NYMC X-179A (California/07/2009 × NYMC X-157)(H1N1)) vaccine virus used in Pandemrix (pooling cells from 2 × 2 mice, n = 2). Recombinant hemagglutinin (rHA) and nucleoprotein (rNP; A/Puerto Rico/8/34 corresponding to the Pandemrix vaccine strain) were used as positive controls. d – f PBMC from Pandemrix-vaccinated HLA-DQB1*0602 positive individuals (sleep clinic patients without a diagnosis of NT1) were stimulated in culture with single 15-mer peptides, derived from the same vaccine virus strain ( n = 1–2). The expression of IFN-γ or IL-2 was measured by FMIA (protein) or RT-qPCR (mRNA). Results are expressed as ratios between cytokine concentrations (lines representing means) or relative gene expressions (dots representing single values, bars representing means) measured in peptide-stimulated and negative control samples (stimulation index). An asterisk (*) indicates a pool or single peptide that was selected for further testing.
    Anti Influenza A H1n1, supplied by Sino Biological, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti influenza a h1n1/product/Sino Biological
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti influenza a h1n1 - by Bioz Stars, 2021-06
    92/100 stars
      Buy from Supplier

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    Influenza A H1N1 virus T-cell epitope screen in HLA-DQ6.2 mice, and Pandemrix-vaccinated HLA-DQB1*0602 positive individuals. Spleen cells from Pandemrix-immunized HLA-DQ6.2 mice were stimulated in culture with pools of five overlapping 15-mer peptides each, covering a hemagglutinin (HA), b neuraminidase (NA) or c nucleoprotein (NP) from influenza (A/reassortant/NYMC X-179A (California/07/2009 × NYMC X-157)(H1N1)) vaccine virus used in Pandemrix (pooling cells from 2 × 2 mice, n = 2). Recombinant hemagglutinin (rHA) and nucleoprotein (rNP; A/Puerto Rico/8/34 corresponding to the Pandemrix vaccine strain) were used as positive controls. d – f PBMC from Pandemrix-vaccinated HLA-DQB1*0602 positive individuals (sleep clinic patients without a diagnosis of NT1) were stimulated in culture with single 15-mer peptides, derived from the same vaccine virus strain ( n = 1–2). The expression of IFN-γ or IL-2 was measured by FMIA (protein) or RT-qPCR (mRNA). Results are expressed as ratios between cytokine concentrations (lines representing means) or relative gene expressions (dots representing single values, bars representing means) measured in peptide-stimulated and negative control samples (stimulation index). An asterisk (*) indicates a pool or single peptide that was selected for further testing.

    Journal: Nature Communications

    Article Title: Enhanced influenza A H1N1 T cell epitope recognition and cross-reactivity to protein-O-mannosyltransferase 1 in Pandemrix-associated narcolepsy type 1

    doi: 10.1038/s41467-021-22637-8

    Figure Lengend Snippet: Influenza A H1N1 virus T-cell epitope screen in HLA-DQ6.2 mice, and Pandemrix-vaccinated HLA-DQB1*0602 positive individuals. Spleen cells from Pandemrix-immunized HLA-DQ6.2 mice were stimulated in culture with pools of five overlapping 15-mer peptides each, covering a hemagglutinin (HA), b neuraminidase (NA) or c nucleoprotein (NP) from influenza (A/reassortant/NYMC X-179A (California/07/2009 × NYMC X-157)(H1N1)) vaccine virus used in Pandemrix (pooling cells from 2 × 2 mice, n = 2). Recombinant hemagglutinin (rHA) and nucleoprotein (rNP; A/Puerto Rico/8/34 corresponding to the Pandemrix vaccine strain) were used as positive controls. d – f PBMC from Pandemrix-vaccinated HLA-DQB1*0602 positive individuals (sleep clinic patients without a diagnosis of NT1) were stimulated in culture with single 15-mer peptides, derived from the same vaccine virus strain ( n = 1–2). The expression of IFN-γ or IL-2 was measured by FMIA (protein) or RT-qPCR (mRNA). Results are expressed as ratios between cytokine concentrations (lines representing means) or relative gene expressions (dots representing single values, bars representing means) measured in peptide-stimulated and negative control samples (stimulation index). An asterisk (*) indicates a pool or single peptide that was selected for further testing.

    Article Snippet: Recombinant influenza A H1N1 (A/California/07/2009) hemagglutinin (cat#11085-V08H), (A/California/04/2009) neuraminidase (cat#11058-VNAHC or cat#11058-V01H), and (A/California/07/2009) or (A/Puerto Rico/8/34/Mount Sinai) nucleoprotein (cat#40205-V08B and cat#11675-V08B) were purchased from Sino Biological, Beijing, China.

    Techniques: Mouse Assay, Recombinant, Derivative Assay, Expressing, Quantitative RT-PCR, Negative Control

    Mapping of identified influenza A H1N1 virus T-cell epitopes in Pandemrix-associated NT1 patients. a , d PBMC from pediatric Pandemrix-associated NT1 patients (NT1; validation cohort) or pediatric Pandemrix-vaccinated healthy controls (C) were stimulated in culture with overlapping 15-mer peptides from influenza (A/reassortant/NYMC X-179A (California/07/2009 × NYMC X-157)(H1N1)) vaccine virus neuraminidase (NA) or nucleoprotein (NP), as indicated. b , c , e , f PBMC from NT1 patients (invariably HLA-DQB1*0602 positive; discovery and validation cohorts combined; HLA-DQB1*0602 homozygous NT1 patients marked with red dots) or HLA-DQB1*0602 positive (C/DQ6+) or negative (C/DQ6−) healthy controls were stimulated with single NA- or NP-derived peptides. The secretion of IFN-γ was measured by FMIA (protein). Results are expressed as the ratio between cytokine concentrations measured in peptide-stimulated and negative control samples (stimulation index). Statistical comparisons between groups were performed, using Kruskal–Wallis and Dunn’s multiple comparisons tests.

    Journal: Nature Communications

    Article Title: Enhanced influenza A H1N1 T cell epitope recognition and cross-reactivity to protein-O-mannosyltransferase 1 in Pandemrix-associated narcolepsy type 1

    doi: 10.1038/s41467-021-22637-8

    Figure Lengend Snippet: Mapping of identified influenza A H1N1 virus T-cell epitopes in Pandemrix-associated NT1 patients. a , d PBMC from pediatric Pandemrix-associated NT1 patients (NT1; validation cohort) or pediatric Pandemrix-vaccinated healthy controls (C) were stimulated in culture with overlapping 15-mer peptides from influenza (A/reassortant/NYMC X-179A (California/07/2009 × NYMC X-157)(H1N1)) vaccine virus neuraminidase (NA) or nucleoprotein (NP), as indicated. b , c , e , f PBMC from NT1 patients (invariably HLA-DQB1*0602 positive; discovery and validation cohorts combined; HLA-DQB1*0602 homozygous NT1 patients marked with red dots) or HLA-DQB1*0602 positive (C/DQ6+) or negative (C/DQ6−) healthy controls were stimulated with single NA- or NP-derived peptides. The secretion of IFN-γ was measured by FMIA (protein). Results are expressed as the ratio between cytokine concentrations measured in peptide-stimulated and negative control samples (stimulation index). Statistical comparisons between groups were performed, using Kruskal–Wallis and Dunn’s multiple comparisons tests.

    Article Snippet: Recombinant influenza A H1N1 (A/California/07/2009) hemagglutinin (cat#11085-V08H), (A/California/04/2009) neuraminidase (cat#11058-VNAHC or cat#11058-V01H), and (A/California/07/2009) or (A/Puerto Rico/8/34/Mount Sinai) nucleoprotein (cat#40205-V08B and cat#11675-V08B) were purchased from Sino Biological, Beijing, China.

    Techniques: Derivative Assay, Negative Control

    Identification of influenza A H1N1 virus T-cell epitopes in Pandemrix-associated NT1 patients. PBMC from pediatric Pandemrix-associated NT1 patients (NT1) or pediatric Pandemrix-vaccinated healthy controls (C) were stimulated in culture with single 15-mer peptides from influenza (A/reassortant/NYMC X-179A (California/07/2009 × NYMC X-157)(H1N1)) vaccine virus hemagglutinin (HA), neuraminidase (NA) or nucleoprotein (NP) (discovery cohort). Recombinant neuraminidase (rNA) and nucleoprotein (rNP) were used as positive controls. The secretion of IFN-γ ( a , c , e ) or IL-2 ( b , d , f ) was measured by FMIA (protein). Results are expressed as the ratio between cytokine concentrations measured in peptide-stimulated and negative control samples (stimulation index). Statistical comparisons between groups were performed, using Kruskal–Wallis and Dunn’s multiple comparisons tests.

    Journal: Nature Communications

    Article Title: Enhanced influenza A H1N1 T cell epitope recognition and cross-reactivity to protein-O-mannosyltransferase 1 in Pandemrix-associated narcolepsy type 1

    doi: 10.1038/s41467-021-22637-8

    Figure Lengend Snippet: Identification of influenza A H1N1 virus T-cell epitopes in Pandemrix-associated NT1 patients. PBMC from pediatric Pandemrix-associated NT1 patients (NT1) or pediatric Pandemrix-vaccinated healthy controls (C) were stimulated in culture with single 15-mer peptides from influenza (A/reassortant/NYMC X-179A (California/07/2009 × NYMC X-157)(H1N1)) vaccine virus hemagglutinin (HA), neuraminidase (NA) or nucleoprotein (NP) (discovery cohort). Recombinant neuraminidase (rNA) and nucleoprotein (rNP) were used as positive controls. The secretion of IFN-γ ( a , c , e ) or IL-2 ( b , d , f ) was measured by FMIA (protein). Results are expressed as the ratio between cytokine concentrations measured in peptide-stimulated and negative control samples (stimulation index). Statistical comparisons between groups were performed, using Kruskal–Wallis and Dunn’s multiple comparisons tests.

    Article Snippet: Recombinant influenza A H1N1 (A/California/07/2009) hemagglutinin (cat#11085-V08H), (A/California/04/2009) neuraminidase (cat#11058-VNAHC or cat#11058-V01H), and (A/California/07/2009) or (A/Puerto Rico/8/34/Mount Sinai) nucleoprotein (cat#40205-V08B and cat#11675-V08B) were purchased from Sino Biological, Beijing, China.

    Techniques: Recombinant, Negative Control