rabbit anti h3n2 ha  (Sino Biological)


Bioz Verified Symbol Sino Biological is a verified supplier
Bioz Manufacturer Symbol Sino Biological manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 91
    Name:
    Influenza A H3N2 Hemagglutinin HA Antibody Rabbit MAb
    Description:
    This antibody was obtained from a rabbit immunized with purified recombinant Influenza A H3N2 A Brisbane 10 2007 Hemagglutinin HA Catalog 11056 V08H ABW23353 1 Met1 Ile531
    Catalog Number:
    11056-R006
    Price:
    None
    Category:
    Primary Antibody
    Reactivity:
    H3N2
    Applications:
    ELISA
    Immunogen:
    Recombinant Influenza A H3N2 (A/Brisbane/10/2007) Hemagglutinin / HA Protein (Catalog#11056-V08H)
    Antibody Type:
    MAb
    Host:
    Rabbit
    Isotype:
    Rabbit IgG
    Buy from Supplier


    Structured Review

    Sino Biological rabbit anti h3n2 ha
    The HA of PR8_HA(MVEKT) and PR8_HA(D-MVEKT) viruses was cleaved in lungs of infected Tmprss2 −/− mice. Female 8–12-week-old WT and Tmprss2 −/− mice were infected intranasally with 2×10 5 f.f.u. PR8, PR8_HA(MVEKT), and PR8_HA(D-MVEKT), and PR8_HA(HK). On day 2 p.i., lungs were homogenized and total protein was quantified. For each sample, 50 µg total protein was run on a SDS-PAGE, blotted to a PVDF membrane and stained by (a) an anti-H1N1 antibody (PR8) or (b) an <t>anti-H3N2</t> antibody (A/Brisbane/10/2007) and both with a HRP-conjugated goat anti-rabbit antibody. HA 0 : uncleaved HA, HA 1 : N-terminal part of cleaved HA. Detection of signals was performed using the FujiFilm LAS-3000 imaging system. Subsequently, the same membrane was incubated with mouse anti-actin, followed by incubation with HRP-conjugated horse anti-mouse IgG and visualized again. Corresponding actin bands are shown below each sample. (c) Cleaved HA is shown as the ratio of HA1*100/(HA1+HA0). Three blots derived from the same lung homogenate samples were analysed using ImageJ software and the mean was depicted ±1 SEM.
    This antibody was obtained from a rabbit immunized with purified recombinant Influenza A H3N2 A Brisbane 10 2007 Hemagglutinin HA Catalog 11056 V08H ABW23353 1 Met1 Ile531
    https://www.bioz.com/result/rabbit anti h3n2 ha/product/Sino Biological
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti h3n2 ha - by Bioz Stars, 2021-04
    91/100 stars

    Images

    1) Product Images from "Exchange of amino acids in the H1-haemagglutinin to H3 residues is required for efficient influenza A virus replication and pathology in Tmprss2 knock-out mice"

    Article Title: Exchange of amino acids in the H1-haemagglutinin to H3 residues is required for efficient influenza A virus replication and pathology in Tmprss2 knock-out mice

    Journal: The Journal of General Virology

    doi: 10.1099/jgv.0.001128

    The HA of PR8_HA(MVEKT) and PR8_HA(D-MVEKT) viruses was cleaved in lungs of infected Tmprss2 −/− mice. Female 8–12-week-old WT and Tmprss2 −/− mice were infected intranasally with 2×10 5 f.f.u. PR8, PR8_HA(MVEKT), and PR8_HA(D-MVEKT), and PR8_HA(HK). On day 2 p.i., lungs were homogenized and total protein was quantified. For each sample, 50 µg total protein was run on a SDS-PAGE, blotted to a PVDF membrane and stained by (a) an anti-H1N1 antibody (PR8) or (b) an anti-H3N2 antibody (A/Brisbane/10/2007) and both with a HRP-conjugated goat anti-rabbit antibody. HA 0 : uncleaved HA, HA 1 : N-terminal part of cleaved HA. Detection of signals was performed using the FujiFilm LAS-3000 imaging system. Subsequently, the same membrane was incubated with mouse anti-actin, followed by incubation with HRP-conjugated horse anti-mouse IgG and visualized again. Corresponding actin bands are shown below each sample. (c) Cleaved HA is shown as the ratio of HA1*100/(HA1+HA0). Three blots derived from the same lung homogenate samples were analysed using ImageJ software and the mean was depicted ±1 SEM.
    Figure Legend Snippet: The HA of PR8_HA(MVEKT) and PR8_HA(D-MVEKT) viruses was cleaved in lungs of infected Tmprss2 −/− mice. Female 8–12-week-old WT and Tmprss2 −/− mice were infected intranasally with 2×10 5 f.f.u. PR8, PR8_HA(MVEKT), and PR8_HA(D-MVEKT), and PR8_HA(HK). On day 2 p.i., lungs were homogenized and total protein was quantified. For each sample, 50 µg total protein was run on a SDS-PAGE, blotted to a PVDF membrane and stained by (a) an anti-H1N1 antibody (PR8) or (b) an anti-H3N2 antibody (A/Brisbane/10/2007) and both with a HRP-conjugated goat anti-rabbit antibody. HA 0 : uncleaved HA, HA 1 : N-terminal part of cleaved HA. Detection of signals was performed using the FujiFilm LAS-3000 imaging system. Subsequently, the same membrane was incubated with mouse anti-actin, followed by incubation with HRP-conjugated horse anti-mouse IgG and visualized again. Corresponding actin bands are shown below each sample. (c) Cleaved HA is shown as the ratio of HA1*100/(HA1+HA0). Three blots derived from the same lung homogenate samples were analysed using ImageJ software and the mean was depicted ±1 SEM.

    Techniques Used: Infection, Mouse Assay, SDS Page, Staining, Imaging, Incubation, Derivative Assay, Software

    Related Articles

    Recombinant:

    Article Title: In situ self-assembly of gold nanoparticles on hydrophilic and hydrophobic substrates for influenza virus-sensing platform
    Article Snippet: The anti-neuraminidase (NA) (New Caledonia/20/1999/(H1N1) rabbit polyclonal antibody was obtained from Immune Technology Corp. (New York, NY, USA). .. Recombinant influenza virus A (New Caledonia/20/1999) (H1N1) (Cat No. 11683-V08H) and influenza A (H3N2) hemagglutinin monoclonal antibodies (Anti-H3N2 antibody HA MAb, Lot: HB04N0160) were purchased from Sino Biological, Inc. (Beijing, China). .. Avian influenza H5N1 neuraminidase polyclonal antibody (Cat No. PA5-34949) was purchased from Thermo Fisher Scientific (Rockford, USA).

    other:

    Article Title: Size-controlled preparation of peroxidase-like graphene-gold nanoparticle hybrids for the visible detection of norovirus-like particles.
    Article Snippet: A hybrid structure of graphene-gold nanoparticles (Grp-Au NPs) was designed as a new nanoprobe for colorimetric immunoassays.

    Staining:

    Article Title: Exchange of amino acids in the H1-haemagglutinin to H3 residues is required for efficient influenza A virus replication and pathology in Tmprss2 knock-out mice
    Article Snippet: Samples were then mixed with 8x SDS loading buffer and 50 µg total protein in lung homogenates or 20 µg total protein in BAL of each sample was run on a 10 % SDS gel. .. Proteins were blotted on a PVDF-membrane (Bio-Rad) and H1 was detected by staining with rabbit anti-H1N1-HA antibody (PR8, Sino biological, 11684-RP01), H3 after staining with rabbit anti-H3N2-HA (A/Brisbane/10/2007, Sino biological, 11056-PR02) or NP protein with anti-NP antibody (GeneTex, GTX125989), all at a dilution of 1 : 5000, followed by incubation with HRP-conjugated goat anti-rabbit antibody in a dilution of 1 : 10 000 (Sigma, A0545). .. Bands were visualized using Western Chemiluminescent HRP substrate (Immobilon) in an imaging system (FujiFilm LAS-3000).

    Incubation:

    Article Title: Exchange of amino acids in the H1-haemagglutinin to H3 residues is required for efficient influenza A virus replication and pathology in Tmprss2 knock-out mice
    Article Snippet: Samples were then mixed with 8x SDS loading buffer and 50 µg total protein in lung homogenates or 20 µg total protein in BAL of each sample was run on a 10 % SDS gel. .. Proteins were blotted on a PVDF-membrane (Bio-Rad) and H1 was detected by staining with rabbit anti-H1N1-HA antibody (PR8, Sino biological, 11684-RP01), H3 after staining with rabbit anti-H3N2-HA (A/Brisbane/10/2007, Sino biological, 11056-PR02) or NP protein with anti-NP antibody (GeneTex, GTX125989), all at a dilution of 1 : 5000, followed by incubation with HRP-conjugated goat anti-rabbit antibody in a dilution of 1 : 10 000 (Sigma, A0545). .. Bands were visualized using Western Chemiluminescent HRP substrate (Immobilon) in an imaging system (FujiFilm LAS-3000).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 91
    Sino Biological rabbit anti h3n2 ha
    The HA of PR8_HA(MVEKT) and PR8_HA(D-MVEKT) viruses was cleaved in lungs of infected Tmprss2 −/− mice. Female 8–12-week-old WT and Tmprss2 −/− mice were infected intranasally with 2×10 5 f.f.u. PR8, PR8_HA(MVEKT), and PR8_HA(D-MVEKT), and PR8_HA(HK). On day 2 p.i., lungs were homogenized and total protein was quantified. For each sample, 50 µg total protein was run on a SDS-PAGE, blotted to a PVDF membrane and stained by (a) an anti-H1N1 antibody (PR8) or (b) an <t>anti-H3N2</t> antibody (A/Brisbane/10/2007) and both with a HRP-conjugated goat anti-rabbit antibody. HA 0 : uncleaved HA, HA 1 : N-terminal part of cleaved HA. Detection of signals was performed using the FujiFilm LAS-3000 imaging system. Subsequently, the same membrane was incubated with mouse anti-actin, followed by incubation with HRP-conjugated horse anti-mouse IgG and visualized again. Corresponding actin bands are shown below each sample. (c) Cleaved HA is shown as the ratio of HA1*100/(HA1+HA0). Three blots derived from the same lung homogenate samples were analysed using ImageJ software and the mean was depicted ±1 SEM.
    Rabbit Anti H3n2 Ha, supplied by Sino Biological, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti h3n2 ha/product/Sino Biological
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti h3n2 ha - by Bioz Stars, 2021-04
    91/100 stars
      Buy from Supplier

    Image Search Results


    The HA of PR8_HA(MVEKT) and PR8_HA(D-MVEKT) viruses was cleaved in lungs of infected Tmprss2 −/− mice. Female 8–12-week-old WT and Tmprss2 −/− mice were infected intranasally with 2×10 5 f.f.u. PR8, PR8_HA(MVEKT), and PR8_HA(D-MVEKT), and PR8_HA(HK). On day 2 p.i., lungs were homogenized and total protein was quantified. For each sample, 50 µg total protein was run on a SDS-PAGE, blotted to a PVDF membrane and stained by (a) an anti-H1N1 antibody (PR8) or (b) an anti-H3N2 antibody (A/Brisbane/10/2007) and both with a HRP-conjugated goat anti-rabbit antibody. HA 0 : uncleaved HA, HA 1 : N-terminal part of cleaved HA. Detection of signals was performed using the FujiFilm LAS-3000 imaging system. Subsequently, the same membrane was incubated with mouse anti-actin, followed by incubation with HRP-conjugated horse anti-mouse IgG and visualized again. Corresponding actin bands are shown below each sample. (c) Cleaved HA is shown as the ratio of HA1*100/(HA1+HA0). Three blots derived from the same lung homogenate samples were analysed using ImageJ software and the mean was depicted ±1 SEM.

    Journal: The Journal of General Virology

    Article Title: Exchange of amino acids in the H1-haemagglutinin to H3 residues is required for efficient influenza A virus replication and pathology in Tmprss2 knock-out mice

    doi: 10.1099/jgv.0.001128

    Figure Lengend Snippet: The HA of PR8_HA(MVEKT) and PR8_HA(D-MVEKT) viruses was cleaved in lungs of infected Tmprss2 −/− mice. Female 8–12-week-old WT and Tmprss2 −/− mice were infected intranasally with 2×10 5 f.f.u. PR8, PR8_HA(MVEKT), and PR8_HA(D-MVEKT), and PR8_HA(HK). On day 2 p.i., lungs were homogenized and total protein was quantified. For each sample, 50 µg total protein was run on a SDS-PAGE, blotted to a PVDF membrane and stained by (a) an anti-H1N1 antibody (PR8) or (b) an anti-H3N2 antibody (A/Brisbane/10/2007) and both with a HRP-conjugated goat anti-rabbit antibody. HA 0 : uncleaved HA, HA 1 : N-terminal part of cleaved HA. Detection of signals was performed using the FujiFilm LAS-3000 imaging system. Subsequently, the same membrane was incubated with mouse anti-actin, followed by incubation with HRP-conjugated horse anti-mouse IgG and visualized again. Corresponding actin bands are shown below each sample. (c) Cleaved HA is shown as the ratio of HA1*100/(HA1+HA0). Three blots derived from the same lung homogenate samples were analysed using ImageJ software and the mean was depicted ±1 SEM.

    Article Snippet: Proteins were blotted on a PVDF-membrane (Bio-Rad) and H1 was detected by staining with rabbit anti-H1N1-HA antibody (PR8, Sino biological, 11684-RP01), H3 after staining with rabbit anti-H3N2-HA (A/Brisbane/10/2007, Sino biological, 11056-PR02) or NP protein with anti-NP antibody (GeneTex, GTX125989), all at a dilution of 1 : 5000, followed by incubation with HRP-conjugated goat anti-rabbit antibody in a dilution of 1 : 10 000 (Sigma, A0545).

    Techniques: Infection, Mouse Assay, SDS Page, Staining, Imaging, Incubation, Derivative Assay, Software