anti h3n2 ha 11056 mm03 50  (Sino Biological)


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    Name:
    Influenza A H3N2 Hemagglutinin HA Antibody Mouse MAb
    Description:
    This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified recombinant Influenza A virus H3N2 Hemagglutinin The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography
    Catalog Number:
    11056-MM03
    Price:
    None
    Category:
    Primary Antibody
    Reactivity:
    H3N2
    Applications:
    WB,ELISA
    Immunogen:
    Recombinant H3N2 HA protein (Catalog#11056-V08H)
    Antibody Type:
    MAb
    Host:
    Mouse
    Isotype:
    Mouse IgG1
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    Structured Review

    Sino Biological anti h3n2 ha 11056 mm03 50
    In vivo protection against influenza infection. ( A and B ) Ab response against influenza’s HA2 α-helix <t>(H3N2</t> HA2 12–63 ). ( A ) BALB/c mice were left untreated or were immunized with equimolar amounts of VHH7 dimer–HA2 12–63 or peptide in solution with free VHH7 ( n = 5 per group). Sera were used for immunoblotting against H3N2/X-31 recombinant HA. Mouse anti-HA and anti-mouse IgG Abs were used as control. ( B ) Sera from mice immunized with VHH7 dimer–HA2 12–63 produce IgG1, as measured by ELISA. Anti-IgG1–HRP was used in the control group (no immunization [no imm]). ( C ) Survival of mice after respiratory challenge with influenza A H3N2/X-31. BALB/c mice previously immunized with VHH7 dimer or VHH7 dimer–HA2 12–63 as in ( A ) were given 1.25 × 10 5 EID 50 of H3N2/X-31 virus and monitored for 12 d postinoculation ( n = 10 per group). Each figure is representative of two to four independent experiments.
    This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified recombinant Influenza A virus H3N2 Hemagglutinin The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography
    https://www.bioz.com/result/anti h3n2 ha 11056 mm03 50/product/Sino Biological
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti h3n2 ha 11056 mm03 50 - by Bioz Stars, 2021-06
    88/100 stars

    Images

    1) Product Images from "Generation of Immunity against Pathogens via Single-Domain Antibody–Antigen Constructs"

    Article Title: Generation of Immunity against Pathogens via Single-Domain Antibody–Antigen Constructs

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.1600692

    In vivo protection against influenza infection. ( A and B ) Ab response against influenza’s HA2 α-helix (H3N2 HA2 12–63 ). ( A ) BALB/c mice were left untreated or were immunized with equimolar amounts of VHH7 dimer–HA2 12–63 or peptide in solution with free VHH7 ( n = 5 per group). Sera were used for immunoblotting against H3N2/X-31 recombinant HA. Mouse anti-HA and anti-mouse IgG Abs were used as control. ( B ) Sera from mice immunized with VHH7 dimer–HA2 12–63 produce IgG1, as measured by ELISA. Anti-IgG1–HRP was used in the control group (no immunization [no imm]). ( C ) Survival of mice after respiratory challenge with influenza A H3N2/X-31. BALB/c mice previously immunized with VHH7 dimer or VHH7 dimer–HA2 12–63 as in ( A ) were given 1.25 × 10 5 EID 50 of H3N2/X-31 virus and monitored for 12 d postinoculation ( n = 10 per group). Each figure is representative of two to four independent experiments.
    Figure Legend Snippet: In vivo protection against influenza infection. ( A and B ) Ab response against influenza’s HA2 α-helix (H3N2 HA2 12–63 ). ( A ) BALB/c mice were left untreated or were immunized with equimolar amounts of VHH7 dimer–HA2 12–63 or peptide in solution with free VHH7 ( n = 5 per group). Sera were used for immunoblotting against H3N2/X-31 recombinant HA. Mouse anti-HA and anti-mouse IgG Abs were used as control. ( B ) Sera from mice immunized with VHH7 dimer–HA2 12–63 produce IgG1, as measured by ELISA. Anti-IgG1–HRP was used in the control group (no immunization [no imm]). ( C ) Survival of mice after respiratory challenge with influenza A H3N2/X-31. BALB/c mice previously immunized with VHH7 dimer or VHH7 dimer–HA2 12–63 as in ( A ) were given 1.25 × 10 5 EID 50 of H3N2/X-31 virus and monitored for 12 d postinoculation ( n = 10 per group). Each figure is representative of two to four independent experiments.

    Techniques Used: In Vivo, Infection, Mouse Assay, Recombinant, Enzyme-linked Immunosorbent Assay

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    Article Title: Colorimetric detection of influenza A virus using antibody-functionalized gold nanoparticles.
    Article Snippet: Early and accurate diagnosis is considered the key issue to prevent the further spread of viruses and facilitate influenza therapy.

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  • 88
    Sino Biological anti h3n2 ha 11056 mm03 50
    In vivo protection against influenza infection. ( A and B ) Ab response against influenza’s HA2 α-helix <t>(H3N2</t> HA2 12–63 ). ( A ) BALB/c mice were left untreated or were immunized with equimolar amounts of VHH7 dimer–HA2 12–63 or peptide in solution with free VHH7 ( n = 5 per group). Sera were used for immunoblotting against H3N2/X-31 recombinant HA. Mouse anti-HA and anti-mouse IgG Abs were used as control. ( B ) Sera from mice immunized with VHH7 dimer–HA2 12–63 produce IgG1, as measured by ELISA. Anti-IgG1–HRP was used in the control group (no immunization [no imm]). ( C ) Survival of mice after respiratory challenge with influenza A H3N2/X-31. BALB/c mice previously immunized with VHH7 dimer or VHH7 dimer–HA2 12–63 as in ( A ) were given 1.25 × 10 5 EID 50 of H3N2/X-31 virus and monitored for 12 d postinoculation ( n = 10 per group). Each figure is representative of two to four independent experiments.
    Anti H3n2 Ha 11056 Mm03 50, supplied by Sino Biological, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti h3n2 ha 11056 mm03 50/product/Sino Biological
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti h3n2 ha 11056 mm03 50 - by Bioz Stars, 2021-06
    88/100 stars
      Buy from Supplier

    Image Search Results


    In vivo protection against influenza infection. ( A and B ) Ab response against influenza’s HA2 α-helix (H3N2 HA2 12–63 ). ( A ) BALB/c mice were left untreated or were immunized with equimolar amounts of VHH7 dimer–HA2 12–63 or peptide in solution with free VHH7 ( n = 5 per group). Sera were used for immunoblotting against H3N2/X-31 recombinant HA. Mouse anti-HA and anti-mouse IgG Abs were used as control. ( B ) Sera from mice immunized with VHH7 dimer–HA2 12–63 produce IgG1, as measured by ELISA. Anti-IgG1–HRP was used in the control group (no immunization [no imm]). ( C ) Survival of mice after respiratory challenge with influenza A H3N2/X-31. BALB/c mice previously immunized with VHH7 dimer or VHH7 dimer–HA2 12–63 as in ( A ) were given 1.25 × 10 5 EID 50 of H3N2/X-31 virus and monitored for 12 d postinoculation ( n = 10 per group). Each figure is representative of two to four independent experiments.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Generation of Immunity against Pathogens via Single-Domain Antibody–Antigen Constructs

    doi: 10.4049/jimmunol.1600692

    Figure Lengend Snippet: In vivo protection against influenza infection. ( A and B ) Ab response against influenza’s HA2 α-helix (H3N2 HA2 12–63 ). ( A ) BALB/c mice were left untreated or were immunized with equimolar amounts of VHH7 dimer–HA2 12–63 or peptide in solution with free VHH7 ( n = 5 per group). Sera were used for immunoblotting against H3N2/X-31 recombinant HA. Mouse anti-HA and anti-mouse IgG Abs were used as control. ( B ) Sera from mice immunized with VHH7 dimer–HA2 12–63 produce IgG1, as measured by ELISA. Anti-IgG1–HRP was used in the control group (no immunization [no imm]). ( C ) Survival of mice after respiratory challenge with influenza A H3N2/X-31. BALB/c mice previously immunized with VHH7 dimer or VHH7 dimer–HA2 12–63 as in ( A ) were given 1.25 × 10 5 EID 50 of H3N2/X-31 virus and monitored for 12 d postinoculation ( n = 10 per group). Each figure is representative of two to four independent experiments.

    Article Snippet: Sera were used at 1:500–3000 dilutions, and secondary Abs were used at a 1:10,000 dilution (anti-mouse IgG-HRP NXA931 [GE Healthcare], anti-His–HRP 1014992 [QIAGEN], anti-H3N2 HA 11056-MM03-50 [Sino Biological]).

    Techniques: In Vivo, Infection, Mouse Assay, Recombinant, Enzyme-linked Immunosorbent Assay