avian influenza virus h5nx pseudovirus  (Sino Biological)


Bioz Verified Symbol Sino Biological is a verified supplier
Bioz Manufacturer Symbol Sino Biological manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90

    Structured Review

    Sino Biological avian influenza virus h5nx pseudovirus
    Lentiviral <t>pseudovirus</t> system of SARS-CoV or SARS-CoV-2 and avian influenza H5. Structural protein genes, including S protein of SARS-CoV or SARS-CoV-2 and HA/NA protein of avian influenza H5, were subcloned into envelope expression plasmid derived from pMD.G vector. To generate SARS-CoV or SARS-CoV-2 and avian influenza <t>H5Nx</t> pseudoviruses, we co-transfected the structural protein expressing either S protein or HA and NA vectors, a package vector, and a reporter vector into HEK-293T cells. Generated SARS-CoV or SARS-CoV-2 and avian influenza H5Nx pseudoviruses were harvested and transduced into Vero-E6 or MDCK cells, respectively.
    Avian Influenza Virus H5nx Pseudovirus, supplied by Sino Biological, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/avian influenza virus h5nx pseudovirus/product/Sino Biological
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    avian influenza virus h5nx pseudovirus - by Bioz Stars, 2021-02
    90/100 stars

    Images

    1) Product Images from "Assessing the application of a pseudovirus system for emerging SARS-CoV-2 and re-emerging avian influenza virus H5 subtypes in vaccine development"

    Article Title: Assessing the application of a pseudovirus system for emerging SARS-CoV-2 and re-emerging avian influenza virus H5 subtypes in vaccine development

    Journal: Biomedical Journal

    doi: 10.1016/j.bj.2020.06.003

    Lentiviral pseudovirus system of SARS-CoV or SARS-CoV-2 and avian influenza H5. Structural protein genes, including S protein of SARS-CoV or SARS-CoV-2 and HA/NA protein of avian influenza H5, were subcloned into envelope expression plasmid derived from pMD.G vector. To generate SARS-CoV or SARS-CoV-2 and avian influenza H5Nx pseudoviruses, we co-transfected the structural protein expressing either S protein or HA and NA vectors, a package vector, and a reporter vector into HEK-293T cells. Generated SARS-CoV or SARS-CoV-2 and avian influenza H5Nx pseudoviruses were harvested and transduced into Vero-E6 or MDCK cells, respectively.
    Figure Legend Snippet: Lentiviral pseudovirus system of SARS-CoV or SARS-CoV-2 and avian influenza H5. Structural protein genes, including S protein of SARS-CoV or SARS-CoV-2 and HA/NA protein of avian influenza H5, were subcloned into envelope expression plasmid derived from pMD.G vector. To generate SARS-CoV or SARS-CoV-2 and avian influenza H5Nx pseudoviruses, we co-transfected the structural protein expressing either S protein or HA and NA vectors, a package vector, and a reporter vector into HEK-293T cells. Generated SARS-CoV or SARS-CoV-2 and avian influenza H5Nx pseudoviruses were harvested and transduced into Vero-E6 or MDCK cells, respectively.

    Techniques Used: Expressing, Plasmid Preparation, Derivative Assay, Transfection, Generated

    Pseudovirus transduction of SARS-CoV or SARS-CoV-2 and avian influenza H5Nx. Generated (A) SARS-CoV or SARS-CoV-2 and (B) avian influenza H5Nx pseudoviruses were transduced into Vero-E6 or MDCK cells, respectively. Red fluorescence indicated the cells transduced by the indicated pseudoviruses with RFP reporter gene. (C) Transduction titers of avian influenza H5Nx pseudoviruses were determined according to the numbers of cells expressing red fluorescence.
    Figure Legend Snippet: Pseudovirus transduction of SARS-CoV or SARS-CoV-2 and avian influenza H5Nx. Generated (A) SARS-CoV or SARS-CoV-2 and (B) avian influenza H5Nx pseudoviruses were transduced into Vero-E6 or MDCK cells, respectively. Red fluorescence indicated the cells transduced by the indicated pseudoviruses with RFP reporter gene. (C) Transduction titers of avian influenza H5Nx pseudoviruses were determined according to the numbers of cells expressing red fluorescence.

    Techniques Used: Transduction, Generated, Fluorescence, Expressing

    Related Articles

    other:

    Article Title: Assessing the application of a pseudovirus system for emerging SARS-CoV-2 and re-emerging avian influenza virus H5 subtypes in vaccine development
    Article Snippet: For avian influenza virus H5Nx pseudovirus, we utilized mouse monoclonal H5N1 HA antibody (11048-MM06, Sino Biological) with 1:1000 dilution.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Sino Biological influenza a h5n1 hemagglutinin ha antibody mouse mab
    Influenza A H5n1 Hemagglutinin Ha Antibody Mouse Mab, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/influenza a h5n1 hemagglutinin ha antibody mouse mab/product/Sino Biological
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    influenza a h5n1 hemagglutinin ha antibody mouse mab - by Bioz Stars, 2021-02
    93/100 stars
      Buy from Supplier

    N/A
    This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified recombinant Human Insulin INS rh
      Buy from Supplier

    N/A
    This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified recombinant Human CD31 PECAM 1
      Buy from Supplier

    Image Search Results