10x taq buffer  (TaKaRa)

 
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    Name:
    10X Lysis Buffer
    Description:
    The 10X Lysis Buffer is a single cell lysis buffer that can be used with the SMARTer Ultra Low Input RNA Kit for Sequencing v3 and the SMART Seq v4 Ultra Low Input RNA Kit for Sequencing
    Catalog Number:
    635013
    Price:
    None
    Size:
    Each
    Category:
    Single cell lysis buffer NGS accessories Next generation sequencing
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    Structured Review

    TaKaRa 10x taq buffer
    The 10X Lysis Buffer is a single cell lysis buffer that can be used with the SMARTer Ultra Low Input RNA Kit for Sequencing v3 and the SMART Seq v4 Ultra Low Input RNA Kit for Sequencing
    https://www.bioz.com/result/10x taq buffer/product/TaKaRa
    Average 99 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    10x taq buffer - by Bioz Stars, 2020-08
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: H19 gene methylation status is associated with male infertility
    Article Snippet: .. All purified PCR products were cloned into pMD18-T vectors (Takara Biotechnology Co., Ltd.) using a TA ligation system (10 µl) containing 0.25 µl pMD18-T vector, 1 µl 10X ligase buffer, 1 µl ligase enzyme (both Takara Biotechnology Co., Ltd.), and 7.75 µl DNA fragments. .. The plasmid was transformed into E. coli DH5α chemically-competent cells (cat. no. CB101-03; Tiangen Biotech Co., Ltd.), which were coated onto a Luria-Bertani agar plate (Sigma-Aldrich) containing 50 µg/ml ampicillin (Promega Corporation, Madison, WI, USA) and cultured overnight at 37°C until white single colonies were observed.

    Ligation:

    Article Title: H19 gene methylation status is associated with male infertility
    Article Snippet: .. All purified PCR products were cloned into pMD18-T vectors (Takara Biotechnology Co., Ltd.) using a TA ligation system (10 µl) containing 0.25 µl pMD18-T vector, 1 µl 10X ligase buffer, 1 µl ligase enzyme (both Takara Biotechnology Co., Ltd.), and 7.75 µl DNA fragments. .. The plasmid was transformed into E. coli DH5α chemically-competent cells (cat. no. CB101-03; Tiangen Biotech Co., Ltd.), which were coated onto a Luria-Bertani agar plate (Sigma-Aldrich) containing 50 µg/ml ampicillin (Promega Corporation, Madison, WI, USA) and cultured overnight at 37°C until white single colonies were observed.

    Purification:

    Article Title: H19 gene methylation status is associated with male infertility
    Article Snippet: .. All purified PCR products were cloned into pMD18-T vectors (Takara Biotechnology Co., Ltd.) using a TA ligation system (10 µl) containing 0.25 µl pMD18-T vector, 1 µl 10X ligase buffer, 1 µl ligase enzyme (both Takara Biotechnology Co., Ltd.), and 7.75 µl DNA fragments. .. The plasmid was transformed into E. coli DH5α chemically-competent cells (cat. no. CB101-03; Tiangen Biotech Co., Ltd.), which were coated onto a Luria-Bertani agar plate (Sigma-Aldrich) containing 50 µg/ml ampicillin (Promega Corporation, Madison, WI, USA) and cultured overnight at 37°C until white single colonies were observed.

    Polymerase Chain Reaction:

    Article Title: A new species and new records of Onchidium slugs ( Gastropoda, Euthyneura, Pulmonata, Onchidiidae) in South-East Asia
    Article Snippet: .. The 25 μl PCRs for COI and 16S contained 15.8 μl of water, 2.5 μl of 10X PCR Buffer, 1.5 μl of 25 mM MgCl2 , 0.5 μl of each 10 μM primer, 2 μl of dNTP Mixture, 0.2 μl (1 unit) of TaKaRa Taq (Code No. R001A), 1 μl of 20 ng/μl template DNA, and 1 μl of 100X BSA (Bovine Serum Albumin). ..

    Article Title: Houttuynia cordata Thunb inhibits the production of pro-inflammatory cytokines through inhibition of the NF?B signaling pathway in HMC-1 human mast cells
    Article Snippet: .. PCR was conducted in a 20 μl reaction mixture consisting of cDNA template, 10 pmol each gene-specific primer, 10X Taq buffer, 2.5 mM dNTP mixture and 1 unit Taq DNA polymerase (Takara Korea Biomedical Inc, Seoul, Korea). .. PCR was performed using the primers: TNF-α, 5′-TGAGCACTGAAAGCATGATCC-3′ and 5′-ATCACTCCAAAGTGCAGCAG-3′; IL-6, 5′-AACC TTTCCAAAGATGGCTGAA-3′ and 5′-CAGGAACTGG ATCAGGACTTT-3′; IL-8, 5′-TCAGTGCATAAAGAC ATACTCC-3′ and 5′-TGGCATCTTCACTGATTCTTG-3′; and GAPDH 5′-CGTCTTCACCACCATGGAGA-3′ and 5′-CGGCCATCACGCCACAGTTT-3′.

    Article Title: Mitochondrial DNA Variability within Uromastyx ornata philbyi (Agamidae: Squamata) from Southwestern Saudi Arabia
    Article Snippet: .. Polymerase chain reaction was set up in a total volume of 10 μ L that contained 1.0 μ L of 10X Fast buffer, 0.8 μ L of 2.5 μ M dNTPs, 1.0 μ L each of 10 μ M primers, 0.05 μ L of SpeedStar HS (5 unit/uL) DNA polymerase (Takara Shuzo Co., Japan), and approximately 1 ng/mL of total genomic DNA. .. PCR was carried out with a Takara thermal cycler using the following cycling program: 32 cycles of denaturation (98°C, 5 s), annealing (55°C, 15 s), and extension (72°C, 20 s).

    Article Title: H19 gene methylation status is associated with male infertility
    Article Snippet: .. All purified PCR products were cloned into pMD18-T vectors (Takara Biotechnology Co., Ltd.) using a TA ligation system (10 µl) containing 0.25 µl pMD18-T vector, 1 µl 10X ligase buffer, 1 µl ligase enzyme (both Takara Biotechnology Co., Ltd.), and 7.75 µl DNA fragments. .. The plasmid was transformed into E. coli DH5α chemically-competent cells (cat. no. CB101-03; Tiangen Biotech Co., Ltd.), which were coated onto a Luria-Bertani agar plate (Sigma-Aldrich) containing 50 µg/ml ampicillin (Promega Corporation, Madison, WI, USA) and cultured overnight at 37°C until white single colonies were observed.

    Lysis:

    Article Title: SMARTer single cell total RNA sequencing
    Article Snippet: .. A375 cells were sorted in 7 μl 1× PBS buffer and Jurkat cells in 8 μl lysis solution [100 μl 10× Lysis buffer (Takara, 635013), 5 μl RNase Inhibitor (Takara, 635013) and 700 μl water]. ..

    Plasmid Preparation:

    Article Title: H19 gene methylation status is associated with male infertility
    Article Snippet: .. All purified PCR products were cloned into pMD18-T vectors (Takara Biotechnology Co., Ltd.) using a TA ligation system (10 µl) containing 0.25 µl pMD18-T vector, 1 µl 10X ligase buffer, 1 µl ligase enzyme (both Takara Biotechnology Co., Ltd.), and 7.75 µl DNA fragments. .. The plasmid was transformed into E. coli DH5α chemically-competent cells (cat. no. CB101-03; Tiangen Biotech Co., Ltd.), which were coated onto a Luria-Bertani agar plate (Sigma-Aldrich) containing 50 µg/ml ampicillin (Promega Corporation, Madison, WI, USA) and cultured overnight at 37°C until white single colonies were observed.

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  • 89
    TaKaRa 10x ex taq buffer
    10x Ex Taq Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 89/100, based on 58 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/10x ex taq buffer/product/TaKaRa
    Average 89 stars, based on 58 article reviews
    Price from $9.99 to $1999.99
    10x ex taq buffer - by Bioz Stars, 2020-08
    89/100 stars
      Buy from Supplier

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