10x t4 pnk buffer  (New England Biolabs)


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    Structured Review

    New England Biolabs 10x t4 pnk buffer
    10x T4 Pnk Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/10x t4 pnk buffer/product/New England Biolabs
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    10x t4 pnk buffer - by Bioz Stars, 2020-04
    94/100 stars

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    Ligation:

    Article Title: Methods designed for the identification and characterization of in vitro and in vivo chromatin assembly mutants in Saccharomyces cerevisiae
    Article Snippet: The Hind III digested pBS is end-labeled by incubating 0.5 ug (in 2 μl) of linear plasmid with 7.5 μl of 32 P-γ-ATP (10 μCi/μl), 1.2 μl of 10X T4 polynucleotide kinase buffer and 1 μl of T4 polynucleotide kinase (New England BioLabs). .. The reaction is incubated at 37°C for one hour and then added to a 500 μl ligation reaction containing the 12 μl kinase reaction, 50 μl 10X T4 ligase buffer and 3 μl T4 ligase (New England BioLabs).

    Labeling:

    Article Title: Methods designed for the identification and characterization of in vitro and in vivo chromatin assembly mutants in Saccharomyces cerevisiae
    Article Snippet: The labeled probe is prepared by digesting 25 μg of pBS with Hind III in a 50 μl reaction. .. The Hind III digested pBS is end-labeled by incubating 0.5 ug (in 2 μl) of linear plasmid with 7.5 μl of 32 P-γ-ATP (10 μCi/μl), 1.2 μl of 10X T4 polynucleotide kinase buffer and 1 μl of T4 polynucleotide kinase (New England BioLabs).

    Purification:

    Article Title: Methods designed for the identification and characterization of in vitro and in vivo chromatin assembly mutants in Saccharomyces cerevisiae
    Article Snippet: The Hind III digested pBS is end-labeled by incubating 0.5 ug (in 2 μl) of linear plasmid with 7.5 μl of 32 P-γ-ATP (10 μCi/μl), 1.2 μl of 10X T4 polynucleotide kinase buffer and 1 μl of T4 polynucleotide kinase (New England BioLabs). .. The next day, ligated, and uniquely labeled pBS is purified by chloroform/phenol and chloroform extraction.

    Concentration Assay:

    Article Title: Methods designed for the identification and characterization of in vitro and in vivo chromatin assembly mutants in Saccharomyces cerevisiae
    Article Snippet: The mixture is incubated at -80°C for 15 minutes, centrifuged for 10 minutes, and the dried pellet resuspended in 1X TE (10 mM Tris-HCl pH 8.0, 1 mM EDTA pH 8.0) at a concentration of 0.25 μg/μl. .. The Hind III digested pBS is end-labeled by incubating 0.5 ug (in 2 μl) of linear plasmid with 7.5 μl of 32 P-γ-ATP (10 μCi/μl), 1.2 μl of 10X T4 polynucleotide kinase buffer and 1 μl of T4 polynucleotide kinase (New England BioLabs).

    Incubation:

    Article Title: Methods designed for the identification and characterization of in vitro and in vivo chromatin assembly mutants in Saccharomyces cerevisiae
    Article Snippet: The mixture is incubated at -80°C for 15 minutes, centrifuged for 10 minutes, and the dried pellet resuspended in 1X TE (10 mM Tris-HCl pH 8.0, 1 mM EDTA pH 8.0) at a concentration of 0.25 μg/μl. .. The Hind III digested pBS is end-labeled by incubating 0.5 ug (in 2 μl) of linear plasmid with 7.5 μl of 32 P-γ-ATP (10 μCi/μl), 1.2 μl of 10X T4 polynucleotide kinase buffer and 1 μl of T4 polynucleotide kinase (New England BioLabs).

    Plasmid Preparation:

    Article Title: Methods designed for the identification and characterization of in vitro and in vivo chromatin assembly mutants in Saccharomyces cerevisiae
    Article Snippet: .. The Hind III digested pBS is end-labeled by incubating 0.5 ug (in 2 μl) of linear plasmid with 7.5 μl of 32 P-γ-ATP (10 μCi/μl), 1.2 μl of 10X T4 polynucleotide kinase buffer and 1 μl of T4 polynucleotide kinase (New England BioLabs). .. The reaction is incubated at 37°C for one hour and then added to a 500 μl ligation reaction containing the 12 μl kinase reaction, 50 μl 10X T4 ligase buffer and 3 μl T4 ligase (New England BioLabs).

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    New England Biolabs t4 polynucleotide kinase buffer
    T4 Polynucleotide Kinase Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 polynucleotide kinase buffer/product/New England Biolabs
    Average 99 stars, based on 23 article reviews
    Price from $9.99 to $1999.99
    t4 polynucleotide kinase buffer - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    94
    New England Biolabs 10x t4 pnk buffer
    10x T4 Pnk Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/10x t4 pnk buffer/product/New England Biolabs
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    10x t4 pnk buffer - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    99
    New England Biolabs t4 pnk buffer
    T4 Pnk Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 pnk buffer/product/New England Biolabs
    Average 99 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    t4 pnk buffer - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

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