10x exonuclease i reaction buffer  (New England Biolabs)


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  • 95
    Name:
    Exonuclease I Reaction Buffer
    Description:
    Exonuclease I Reaction Buffer 6 0 ml
    Catalog Number:
    b0293s
    Price:
    24
    Size:
    6 0 ml
    Category:
    Buffers
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    Structured Review

    New England Biolabs 10x exonuclease i reaction buffer
    Exonuclease I Reaction Buffer
    Exonuclease I Reaction Buffer 6 0 ml
    https://www.bioz.com/result/10x exonuclease i reaction buffer/product/New England Biolabs
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    10x exonuclease i reaction buffer - by Bioz Stars, 2020-07
    95/100 stars

    Related Products / Commonly Used Together

    exonuclease i enzyme
    exonuclease-treated
    cdna

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Light-activated cell identification and sorting (LACIS) for selection of edited clones on a nanofluidic device
    Article Snippet: .. Excess PCR primers were removed by incubating with Exonuclease I (NEB, M0293S) in 1 × exonuclease reaction buffer (NEB, B0293S) for 1 h at 37 °C, followed by enzyme inactivation for 20 min at 80 °C. .. Amplicon pools were re-amplified by PCR for 15 cycles using a universal primer to add the sequencing adaptor and secondary barcodes to allow parallel sequencing of multiple amplicon pools (Primer sequences are listed in Supplementary Data ).

    Article Title: Decontamination of 16S rRNA gene amplicon sequence datasets based on bacterial load assessment by qPCR
    Article Snippet: .. The primers from the first round of PCR were removed by digesting 5-μl samples with 1 unit Exonuclease I (New England Biolabs, Ipswich, MA, USA) in a total volume of 10 μl Exonuclease I Reaction Buffer (New England Biolabs) at 37 °C for 30 min. .. The enzyme was inactivated at 95 °C for 15 min. Amplicon barcoding was performed by re-amplification using 1 μl of Exonuclease I-treated first-round PCR, 15 pmol each of forward primer 5’-NNNNNNNNNNTCCTACGGGNGGCWGCAG-3’ and reverse primer 5’- NNNNNNNNNNTGACTACHVGGGTATCTAAKCC-3’ in a 20-μL volume of MyTaq buffer that contained 1.5 units MyTaq DNA polymerase (Bioline, London, UK) and 2 μl of BioStab PCR optimizer (II) (Sigma-Aldrich, Munich, Germany).

    Article Title: ChIP-seq: using high-throughput sequencing to discover protein-DNA interactions
    Article Snippet: .. Exonuclease digestion of linkers on phosphorylated LMPCR library: Mix directly on ice in a PCR tube: 8.5 μl sample; 1 μl 10 × reaction buffer (NEB, B0262S) and 0.5 μl Lambda exonuclease (NEB, M0262S). .. Digest for 10 min at 37 °C in a PCR block.

    other:

    Article Title: Autophagy compensates for defects in mitochondrial dynamics
    Article Snippet: The Exonuclease I mix contained 0.2 μL of Exonuclease I reaction buffer (New England BioLabs), 0.4 μL Exonuclease I at 20 Units/μL (New England BioLabs), and 1.4 μL of nuclease free water.

    Article Title: Spatial distribution and function of T follicular regulatory cells in human lymph nodes
    Article Snippet: Preamplified samples were subjected to an exonuclease reaction, using Exonuclease I and Exonuclease I Reaction Buffer (New England Biolabs).

    Article Title: Transcriptional Regulation and Mechanism of SigN (ZpdN), a pBS32-Encoded Sigma Factor in Bacillus subtilis
    Article Snippet: An assembly master mixture was made by combining prepared 5× isothermal assembly reaction buffer (131 mM Tris-HCl, 13.1 mM MgCl2 , 13.1 mM DTT, 8.21 mM PEG 8000, 1.32 mM NAD, and 0.26 mM [each] dNTP) with Phusion DNA polymerase (New England BioLabs) (0.033 units/μl), T5 exonuclease diluted 1:5 with 5× reaction buffer (New England BioLabs) (0.01 units/μl), Taq DNA ligase (New England BioLabs) (5,328 units/μl), and additional dNTPs (267 μM).

    Article Title: Limited immune surveillance in lymphoid tissue by cytolytic CD4+ T cells during health and HIV disease
    Article Snippet: Exonuclease mixture, containing of Exonuclease I (New England BioLabs), 10× Exonuclease I Reaction Buffer (New England BioLabs) and H2 O was added to each well to remove excessive primers and the plate was run on a thermocycler (37°C for 30 min, 80°C for 15 min).

    Article Title: Detection of 16S rRNA and KPC Genes from Complex Matrix Utilizing a Molecular Inversion Probe Assay for Next-Generation Sequencing
    Article Snippet: For the exonuclease reaction 20 units of exonuclease I (NEB), 25 units of exonuclease III (NEB), and water were added to the reaction mixture up to a final volume to 11.5 μl.

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  • 95
    New England Biolabs 10x exonuclease i reaction buffer
    10x Exonuclease I Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/10x exonuclease i reaction buffer/product/New England Biolabs
    Average 95 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    10x exonuclease i reaction buffer - by Bioz Stars, 2020-07
    95/100 stars
      Buy from Supplier

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