10x antarctic phosphatase reaction buffer  (New England Biolabs)


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    Name:
    Antarctic Phosphatase Reaction Buffer
    Description:
    Antarctic Phosphatase Reaction Buffer 6 0 ml
    Catalog Number:
    b0289s
    Price:
    24
    Size:
    6 0 ml
    Category:
    Buffers
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    Structured Review

    New England Biolabs 10x antarctic phosphatase reaction buffer
    Antarctic Phosphatase Reaction Buffer
    Antarctic Phosphatase Reaction Buffer 6 0 ml
    https://www.bioz.com/result/10x antarctic phosphatase reaction buffer/product/New England Biolabs
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    10x antarctic phosphatase reaction buffer - by Bioz Stars, 2020-05
    93/100 stars

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    Related Articles

    Ethanol Precipitation:

    Article Title: Mapping 3D genome organization relative to nuclear compartments using TSA-Seq as a cytological ruler
    Article Snippet: .. Lamin B1 DamID DamID of lamin B1 was performed in K562 cells as described ( ; ) except that before the DpnI digestion, 1.5 µg genomic DNA was treated for 1 h with 5 U Antarctic phosphatase (M0289S; NEB) in 20 µl 1× Antarctic phosphatase reaction buffer (NEB) followed by ethanol precipitation. .. This additional step prevents ligation of the adaptor to DNA fragments originating from apoptotic cells.

    Purification:

    Article Title: Comparison of CAGE and RNA-seq transcriptome profiling using clonally amplified and single-molecule next-generation sequencing
    Article Snippet: .. Purified RNA was dephosphorylated by adding 2 μL of 10× phosphatase buffer, 5 units of Antarctic phosphatase (NEB), and 40 units of RNaseOUT (Invitrogen) and incubating for 30 min at 37°C followed by 5 min at 65°C. .. After incubation, the sample was set on ice and 5 μL of 10× PNK buffer, 20 units of T4 polynucleotide kinase (NEB), 5 μL of 10 mM ATP (Epicentre), 40 units of RNaseOUT, and 17 μL of water were added, and incubated at 37°C for 60 min. Phosphorated RNA was purified with the RNeasy MinElute kit (Qiagen) as described before.

    Concentration Assay:

    Article Title: Transcriptome maps of general eukaryotic RNA degradation factors
    Article Snippet: .. The dephosphorylation reaction was performed in antarctic phosphatase reaction buffer (NEB, Germany) supplemented with 1 U/µL of antarctic phosphatase and 1 U/µL of RNase OUT (Invitrogen) at 37°C for 30 min. For rephosphyorylation, beads were incubated in T4 PNK reaction buffer A (Invitrogen) with a final concentration of 1 U/µL T4 PNK, 1 U/µL RNase OUT and 1 mM ATP for 1 hr at 37°C. .. 3´ adapter ligation was performed in T4 RNA ligase buffer (NEB) with 10 U/µL T4 RNA ligase 2 (KQ) (NEB), 10 μM 3′ adapter (5′ 5rApp-TGGAATTCTCGGGTGCCAAGG-3ddC 3′ (IDT), 1 U/µL RNase OUT, and 15% (w/v) PEG 8000 overnight at 16°C.

    Incubation:

    Article Title: mRNA Transfection of Mouse and Human Neural Stem Cell Cultures
    Article Snippet: .. To remove 5′ triphosphate moieties from uncapped transcripts, 10 µl of Antarctic Phosphatase reaction buffer and 5 µl (25U) of Antarctic Phosphatase (New England BioLabs) was added to each RNA product and incubated for 30-minutes at 37°C. .. Synthesized RNA products were then repurified using RNeasy Mini Columns (Qiagen) and quantitated by spectrophotometry and denaturing gel electrophoresis.

    Article Title: Transcriptome maps of general eukaryotic RNA degradation factors
    Article Snippet: .. The dephosphorylation reaction was performed in antarctic phosphatase reaction buffer (NEB, Germany) supplemented with 1 U/µL of antarctic phosphatase and 1 U/µL of RNase OUT (Invitrogen) at 37°C for 30 min. For rephosphyorylation, beads were incubated in T4 PNK reaction buffer A (Invitrogen) with a final concentration of 1 U/µL T4 PNK, 1 U/µL RNase OUT and 1 mM ATP for 1 hr at 37°C. .. 3´ adapter ligation was performed in T4 RNA ligase buffer (NEB) with 10 U/µL T4 RNA ligase 2 (KQ) (NEB), 10 μM 3′ adapter (5′ 5rApp-TGGAATTCTCGGGTGCCAAGG-3ddC 3′ (IDT), 1 U/µL RNase OUT, and 15% (w/v) PEG 8000 overnight at 16°C.

    Article Title: RNA-dependent chromatin association of transcription elongation factors and Pol II CTD kinases
    Article Snippet: .. For dephosphorylation, 1× antarctic phosphatase reaction buffer (NEB, Germany) with 1 U/µL of antarctic phosphatase and 1 U/µL of RNase OUT (Invitrogen) were added and the suspension was incubated at 37°C for 30 min and 800 rpm. ..

    other:

    Article Title: Predicting Silk Fiber Mechanical Properties through Multiscale Simulation and Protein Design
    Article Snippet: NEB endonuclease restriction enzyme buffer set (New England Biolabs, Ipswich, MA, USA) DNA Ligase Buffer (New England Biolabs, Ipswich, MA, USA) TAE (Tris-acetate-EDTA) buffer (Fisher Scientific, Pittsburgh, PA, USA) 10X Antarctic Phosphatase Reaction Buffer (New England Biolabs, Ipswich, MA, USA) NuPAGE MES SDS Running Buffer (20X) (ThermoFisher Scientific, Waltham, MA, USA) Denaturing buffer (8 M urea, 0.1 M NaH2PO4, 0.01 M Tris-HCl, pH 8.0)

    De-Phosphorylation Assay:

    Article Title: Transcriptome maps of general eukaryotic RNA degradation factors
    Article Snippet: .. The dephosphorylation reaction was performed in antarctic phosphatase reaction buffer (NEB, Germany) supplemented with 1 U/µL of antarctic phosphatase and 1 U/µL of RNase OUT (Invitrogen) at 37°C for 30 min. For rephosphyorylation, beads were incubated in T4 PNK reaction buffer A (Invitrogen) with a final concentration of 1 U/µL T4 PNK, 1 U/µL RNase OUT and 1 mM ATP for 1 hr at 37°C. .. 3´ adapter ligation was performed in T4 RNA ligase buffer (NEB) with 10 U/µL T4 RNA ligase 2 (KQ) (NEB), 10 μM 3′ adapter (5′ 5rApp-TGGAATTCTCGGGTGCCAAGG-3ddC 3′ (IDT), 1 U/µL RNase OUT, and 15% (w/v) PEG 8000 overnight at 16°C.

    Article Title: RNA-dependent chromatin association of transcription elongation factors and Pol II CTD kinases
    Article Snippet: .. For dephosphorylation, 1× antarctic phosphatase reaction buffer (NEB, Germany) with 1 U/µL of antarctic phosphatase and 1 U/µL of RNase OUT (Invitrogen) were added and the suspension was incubated at 37°C for 30 min and 800 rpm. ..

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  • 99
    New England Biolabs antarctic phosphatase 10x reaction buffer
    Antarctic Phosphatase 10x Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 444 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antarctic phosphatase 10x reaction buffer/product/New England Biolabs
    Average 99 stars, based on 444 article reviews
    Price from $9.99 to $1999.99
    antarctic phosphatase 10x reaction buffer - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    93
    New England Biolabs 10x antarctic phosphatase reaction buffer
    10x Antarctic Phosphatase Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/10x antarctic phosphatase reaction buffer/product/New England Biolabs
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    10x antarctic phosphatase reaction buffer - by Bioz Stars, 2020-05
    93/100 stars
      Buy from Supplier

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