human recombinant fgf10  (Sino Biological)


Bioz Verified Symbol Sino Biological is a verified supplier
Bioz Manufacturer Symbol Sino Biological manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 91
    Name:
    FGF10 Protein Human Recombinant
    Description:
    A DNA sequence encoding the mature form of human FGF10 NP 004456 1 Gln 38 Ser 208 was expressed
    Catalog Number:
    10573-HNAE
    Price:
    None
    Category:
    recombinant protein
    Product Aliases:
    KGF2 Protein Human
    Host:
    E. coli
    Buy from Supplier


    Structured Review

    Sino Biological human recombinant fgf10
    ERK1/2 signaling regulates the expression of Anxa4 . ( A ) Diagram illustrating the method for mesenchyme-free lung endoderm culture. Whole lung at E11.5 was digested with trypsin on ice and then the mesenchyme was removed using fine forceps to get intact lung endoderm. The distal lung endoderm buds were dissected out, embedded in Matrigel and cultured in serum-free medium containing recombinant <t>FGF10.</t> ( B ) Images of cultured lung endoderm taken at different time points. There is no obvious bud formation at 24 h after culture. By 48 h, the lung endoderm starts budding and forms many buds. Scale bar: 200 μm. ( C ) Western blot of cultured lung endoderm explants at 0 h, 24 h and 48 h using antibodies against p-ERK1/2, total ERK1/2 and reference Actin. The phosphorylation level of ERK1/2 was increased significantly at both 24 h and 48 h. Full-length blots are presented in Supplementary Fig. s6 . ( D ) Bud of cultured lung endoderm were analyzed by E-cadherin (red) and p-ERK1/2 (green) immunostaining. The bud tips (arrowhead) showed higher p-ERK1/2 staining compared to middle cells. Scale bar: 25 μm. ( E ) Whole-mount and section in situ hybridization of Anxa4 in WT lungs at E12.5. Anxa4 was highly expressed in the bud tip epithelial cells adjacent to FGF10 expressing site. Scale bar: 200 μm. ( F , G ) The expression level of Anxa4 in cultured lung endoderm explants at 0 h, 24 h and 48 h. The expression level of Anxa4 was increased significantly at both 24 h and 48 h as compared to 0 h and its expression level was inhibited by MEK inhibitor, PD0325901 ( F ). The whole mount in situ hybridization of Anxa4 confirmed that PD0325901 treatment could decrease the expression of Anxa4 ( G ). Data are presented as mean ± SEM, n = 3; **p
    A DNA sequence encoding the mature form of human FGF10 NP 004456 1 Gln 38 Ser 208 was expressed
    https://www.bioz.com/result/human recombinant fgf10/product/Sino Biological
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human recombinant fgf10 - by Bioz Stars, 2021-05
    91/100 stars

    Images

    1) Product Images from "Anxa4 mediated airway progenitor cell migration promotes distal epithelial cell fate specification"

    Article Title: Anxa4 mediated airway progenitor cell migration promotes distal epithelial cell fate specification

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-32494-z

    ERK1/2 signaling regulates the expression of Anxa4 . ( A ) Diagram illustrating the method for mesenchyme-free lung endoderm culture. Whole lung at E11.5 was digested with trypsin on ice and then the mesenchyme was removed using fine forceps to get intact lung endoderm. The distal lung endoderm buds were dissected out, embedded in Matrigel and cultured in serum-free medium containing recombinant FGF10. ( B ) Images of cultured lung endoderm taken at different time points. There is no obvious bud formation at 24 h after culture. By 48 h, the lung endoderm starts budding and forms many buds. Scale bar: 200 μm. ( C ) Western blot of cultured lung endoderm explants at 0 h, 24 h and 48 h using antibodies against p-ERK1/2, total ERK1/2 and reference Actin. The phosphorylation level of ERK1/2 was increased significantly at both 24 h and 48 h. Full-length blots are presented in Supplementary Fig. s6 . ( D ) Bud of cultured lung endoderm were analyzed by E-cadherin (red) and p-ERK1/2 (green) immunostaining. The bud tips (arrowhead) showed higher p-ERK1/2 staining compared to middle cells. Scale bar: 25 μm. ( E ) Whole-mount and section in situ hybridization of Anxa4 in WT lungs at E12.5. Anxa4 was highly expressed in the bud tip epithelial cells adjacent to FGF10 expressing site. Scale bar: 200 μm. ( F , G ) The expression level of Anxa4 in cultured lung endoderm explants at 0 h, 24 h and 48 h. The expression level of Anxa4 was increased significantly at both 24 h and 48 h as compared to 0 h and its expression level was inhibited by MEK inhibitor, PD0325901 ( F ). The whole mount in situ hybridization of Anxa4 confirmed that PD0325901 treatment could decrease the expression of Anxa4 ( G ). Data are presented as mean ± SEM, n = 3; **p
    Figure Legend Snippet: ERK1/2 signaling regulates the expression of Anxa4 . ( A ) Diagram illustrating the method for mesenchyme-free lung endoderm culture. Whole lung at E11.5 was digested with trypsin on ice and then the mesenchyme was removed using fine forceps to get intact lung endoderm. The distal lung endoderm buds were dissected out, embedded in Matrigel and cultured in serum-free medium containing recombinant FGF10. ( B ) Images of cultured lung endoderm taken at different time points. There is no obvious bud formation at 24 h after culture. By 48 h, the lung endoderm starts budding and forms many buds. Scale bar: 200 μm. ( C ) Western blot of cultured lung endoderm explants at 0 h, 24 h and 48 h using antibodies against p-ERK1/2, total ERK1/2 and reference Actin. The phosphorylation level of ERK1/2 was increased significantly at both 24 h and 48 h. Full-length blots are presented in Supplementary Fig. s6 . ( D ) Bud of cultured lung endoderm were analyzed by E-cadherin (red) and p-ERK1/2 (green) immunostaining. The bud tips (arrowhead) showed higher p-ERK1/2 staining compared to middle cells. Scale bar: 25 μm. ( E ) Whole-mount and section in situ hybridization of Anxa4 in WT lungs at E12.5. Anxa4 was highly expressed in the bud tip epithelial cells adjacent to FGF10 expressing site. Scale bar: 200 μm. ( F , G ) The expression level of Anxa4 in cultured lung endoderm explants at 0 h, 24 h and 48 h. The expression level of Anxa4 was increased significantly at both 24 h and 48 h as compared to 0 h and its expression level was inhibited by MEK inhibitor, PD0325901 ( F ). The whole mount in situ hybridization of Anxa4 confirmed that PD0325901 treatment could decrease the expression of Anxa4 ( G ). Data are presented as mean ± SEM, n = 3; **p

    Techniques Used: Expressing, Cell Culture, Recombinant, Western Blot, Immunostaining, Staining, In Situ Hybridization

    2) Product Images from "Anxa4 mediated airway progenitor cell migration promotes distal epithelial cell fate specification"

    Article Title: Anxa4 mediated airway progenitor cell migration promotes distal epithelial cell fate specification

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-32494-z

    ERK1/2 signaling regulates the expression of Anxa4 . ( A ) Diagram illustrating the method for mesenchyme-free lung endoderm culture. Whole lung at E11.5 was digested with trypsin on ice and then the mesenchyme was removed using fine forceps to get intact lung endoderm. The distal lung endoderm buds were dissected out, embedded in Matrigel and cultured in serum-free medium containing recombinant FGF10. ( B ) Images of cultured lung endoderm taken at different time points. There is no obvious bud formation at 24 h after culture. By 48 h, the lung endoderm starts budding and forms many buds. Scale bar: 200 μm. ( C ) Western blot of cultured lung endoderm explants at 0 h, 24 h and 48 h using antibodies against p-ERK1/2, total ERK1/2 and reference Actin. The phosphorylation level of ERK1/2 was increased significantly at both 24 h and 48 h. Full-length blots are presented in Supplementary Fig. s6 . ( D ) Bud of cultured lung endoderm were analyzed by E-cadherin (red) and p-ERK1/2 (green) immunostaining. The bud tips (arrowhead) showed higher p-ERK1/2 staining compared to middle cells. Scale bar: 25 μm. ( E ) Whole-mount and section in situ hybridization of Anxa4 in WT lungs at E12.5. Anxa4 was highly expressed in the bud tip epithelial cells adjacent to FGF10 expressing site. Scale bar: 200 μm. ( F , G ) The expression level of Anxa4 in cultured lung endoderm explants at 0 h, 24 h and 48 h. The expression level of Anxa4 was increased significantly at both 24 h and 48 h as compared to 0 h and its expression level was inhibited by MEK inhibitor, PD0325901 ( F ). The whole mount in situ hybridization of Anxa4 confirmed that PD0325901 treatment could decrease the expression of Anxa4 ( G ). Data are presented as mean ± SEM, n = 3; **p
    Figure Legend Snippet: ERK1/2 signaling regulates the expression of Anxa4 . ( A ) Diagram illustrating the method for mesenchyme-free lung endoderm culture. Whole lung at E11.5 was digested with trypsin on ice and then the mesenchyme was removed using fine forceps to get intact lung endoderm. The distal lung endoderm buds were dissected out, embedded in Matrigel and cultured in serum-free medium containing recombinant FGF10. ( B ) Images of cultured lung endoderm taken at different time points. There is no obvious bud formation at 24 h after culture. By 48 h, the lung endoderm starts budding and forms many buds. Scale bar: 200 μm. ( C ) Western blot of cultured lung endoderm explants at 0 h, 24 h and 48 h using antibodies against p-ERK1/2, total ERK1/2 and reference Actin. The phosphorylation level of ERK1/2 was increased significantly at both 24 h and 48 h. Full-length blots are presented in Supplementary Fig. s6 . ( D ) Bud of cultured lung endoderm were analyzed by E-cadherin (red) and p-ERK1/2 (green) immunostaining. The bud tips (arrowhead) showed higher p-ERK1/2 staining compared to middle cells. Scale bar: 25 μm. ( E ) Whole-mount and section in situ hybridization of Anxa4 in WT lungs at E12.5. Anxa4 was highly expressed in the bud tip epithelial cells adjacent to FGF10 expressing site. Scale bar: 200 μm. ( F , G ) The expression level of Anxa4 in cultured lung endoderm explants at 0 h, 24 h and 48 h. The expression level of Anxa4 was increased significantly at both 24 h and 48 h as compared to 0 h and its expression level was inhibited by MEK inhibitor, PD0325901 ( F ). The whole mount in situ hybridization of Anxa4 confirmed that PD0325901 treatment could decrease the expression of Anxa4 ( G ). Data are presented as mean ± SEM, n = 3; **p

    Techniques Used: Expressing, Cell Culture, Recombinant, Western Blot, Immunostaining, Staining, In Situ Hybridization

    Related Articles

    Migration:

    Article Title: Anxa4 mediated airway progenitor cell migration promotes distal epithelial cell fate specification
    Article Snippet: The supernatant was collected and centrifuged for 3 min at 350 × g to obtain purified primary lung epithelial cells, which were suspended with DMEM/F12 plus 1% FBS and quantified (cell number) using a hemocytometer. .. For the Transwell® migration assays, 5 × 104 cells in 200 μl were seeded into the upper well of the Transwell® apparatus (6.5 mm diameter, 8 μm pore size, Corning Costar); 600 μl of DMEM/F12 medium supplemented with 400 ng/ml human recombinant FGF10 was added into the lower chamber. .. Following incubation for 48 hours at 37 °C to allow cell migration, cells were fixed with 4% PFA and perform crystal violet staining.

    Recombinant:

    Article Title: Anxa4 mediated airway progenitor cell migration promotes distal epithelial cell fate specification
    Article Snippet: The supernatant was collected and centrifuged for 3 min at 350 × g to obtain purified primary lung epithelial cells, which were suspended with DMEM/F12 plus 1% FBS and quantified (cell number) using a hemocytometer. .. For the Transwell® migration assays, 5 × 104 cells in 200 μl were seeded into the upper well of the Transwell® apparatus (6.5 mm diameter, 8 μm pore size, Corning Costar); 600 μl of DMEM/F12 medium supplemented with 400 ng/ml human recombinant FGF10 was added into the lower chamber. .. Following incubation for 48 hours at 37 °C to allow cell migration, cells were fixed with 4% PFA and perform crystal violet staining.

    Article Title: Anxa4 mediated airway progenitor cell migration promotes distal epithelial cell fate specification
    Article Snippet: Lung endoderm explants were isolated from the mesenchyme using tungsten needles in DMEM/F12 media with 10% fetal bovine serum. .. The distal lung endoderm buds were cut off and embedded in 50% growth factor reduced Matrigel (Corning) and cultured in DMEM/F12 media supplemented with 800 ng/ml human recombinant FGF10 (Sino Biological). .. In some experiments, a MEK inhibitor (PD0325901, 1 μM, Selleck) was added at 1 h after culture initiation.

    Cell Culture:

    Article Title: Anxa4 mediated airway progenitor cell migration promotes distal epithelial cell fate specification
    Article Snippet: Lung endoderm explants were isolated from the mesenchyme using tungsten needles in DMEM/F12 media with 10% fetal bovine serum. .. The distal lung endoderm buds were cut off and embedded in 50% growth factor reduced Matrigel (Corning) and cultured in DMEM/F12 media supplemented with 800 ng/ml human recombinant FGF10 (Sino Biological). .. In some experiments, a MEK inhibitor (PD0325901, 1 μM, Selleck) was added at 1 h after culture initiation.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 91
    Sino Biological human recombinant fgf10
    ERK1/2 signaling regulates the expression of Anxa4 . ( A ) Diagram illustrating the method for mesenchyme-free lung endoderm culture. Whole lung at E11.5 was digested with trypsin on ice and then the mesenchyme was removed using fine forceps to get intact lung endoderm. The distal lung endoderm buds were dissected out, embedded in Matrigel and cultured in serum-free medium containing recombinant <t>FGF10.</t> ( B ) Images of cultured lung endoderm taken at different time points. There is no obvious bud formation at 24 h after culture. By 48 h, the lung endoderm starts budding and forms many buds. Scale bar: 200 μm. ( C ) Western blot of cultured lung endoderm explants at 0 h, 24 h and 48 h using antibodies against p-ERK1/2, total ERK1/2 and reference Actin. The phosphorylation level of ERK1/2 was increased significantly at both 24 h and 48 h. Full-length blots are presented in Supplementary Fig. s6 . ( D ) Bud of cultured lung endoderm were analyzed by E-cadherin (red) and p-ERK1/2 (green) immunostaining. The bud tips (arrowhead) showed higher p-ERK1/2 staining compared to middle cells. Scale bar: 25 μm. ( E ) Whole-mount and section in situ hybridization of Anxa4 in WT lungs at E12.5. Anxa4 was highly expressed in the bud tip epithelial cells adjacent to FGF10 expressing site. Scale bar: 200 μm. ( F , G ) The expression level of Anxa4 in cultured lung endoderm explants at 0 h, 24 h and 48 h. The expression level of Anxa4 was increased significantly at both 24 h and 48 h as compared to 0 h and its expression level was inhibited by MEK inhibitor, PD0325901 ( F ). The whole mount in situ hybridization of Anxa4 confirmed that PD0325901 treatment could decrease the expression of Anxa4 ( G ). Data are presented as mean ± SEM, n = 3; **p
    Human Recombinant Fgf10, supplied by Sino Biological, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human recombinant fgf10/product/Sino Biological
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human recombinant fgf10 - by Bioz Stars, 2021-05
    91/100 stars
      Buy from Supplier

    Image Search Results


    ERK1/2 signaling regulates the expression of Anxa4 . ( A ) Diagram illustrating the method for mesenchyme-free lung endoderm culture. Whole lung at E11.5 was digested with trypsin on ice and then the mesenchyme was removed using fine forceps to get intact lung endoderm. The distal lung endoderm buds were dissected out, embedded in Matrigel and cultured in serum-free medium containing recombinant FGF10. ( B ) Images of cultured lung endoderm taken at different time points. There is no obvious bud formation at 24 h after culture. By 48 h, the lung endoderm starts budding and forms many buds. Scale bar: 200 μm. ( C ) Western blot of cultured lung endoderm explants at 0 h, 24 h and 48 h using antibodies against p-ERK1/2, total ERK1/2 and reference Actin. The phosphorylation level of ERK1/2 was increased significantly at both 24 h and 48 h. Full-length blots are presented in Supplementary Fig. s6 . ( D ) Bud of cultured lung endoderm were analyzed by E-cadherin (red) and p-ERK1/2 (green) immunostaining. The bud tips (arrowhead) showed higher p-ERK1/2 staining compared to middle cells. Scale bar: 25 μm. ( E ) Whole-mount and section in situ hybridization of Anxa4 in WT lungs at E12.5. Anxa4 was highly expressed in the bud tip epithelial cells adjacent to FGF10 expressing site. Scale bar: 200 μm. ( F , G ) The expression level of Anxa4 in cultured lung endoderm explants at 0 h, 24 h and 48 h. The expression level of Anxa4 was increased significantly at both 24 h and 48 h as compared to 0 h and its expression level was inhibited by MEK inhibitor, PD0325901 ( F ). The whole mount in situ hybridization of Anxa4 confirmed that PD0325901 treatment could decrease the expression of Anxa4 ( G ). Data are presented as mean ± SEM, n = 3; **p

    Journal: Scientific Reports

    Article Title: Anxa4 mediated airway progenitor cell migration promotes distal epithelial cell fate specification

    doi: 10.1038/s41598-018-32494-z

    Figure Lengend Snippet: ERK1/2 signaling regulates the expression of Anxa4 . ( A ) Diagram illustrating the method for mesenchyme-free lung endoderm culture. Whole lung at E11.5 was digested with trypsin on ice and then the mesenchyme was removed using fine forceps to get intact lung endoderm. The distal lung endoderm buds were dissected out, embedded in Matrigel and cultured in serum-free medium containing recombinant FGF10. ( B ) Images of cultured lung endoderm taken at different time points. There is no obvious bud formation at 24 h after culture. By 48 h, the lung endoderm starts budding and forms many buds. Scale bar: 200 μm. ( C ) Western blot of cultured lung endoderm explants at 0 h, 24 h and 48 h using antibodies against p-ERK1/2, total ERK1/2 and reference Actin. The phosphorylation level of ERK1/2 was increased significantly at both 24 h and 48 h. Full-length blots are presented in Supplementary Fig. s6 . ( D ) Bud of cultured lung endoderm were analyzed by E-cadherin (red) and p-ERK1/2 (green) immunostaining. The bud tips (arrowhead) showed higher p-ERK1/2 staining compared to middle cells. Scale bar: 25 μm. ( E ) Whole-mount and section in situ hybridization of Anxa4 in WT lungs at E12.5. Anxa4 was highly expressed in the bud tip epithelial cells adjacent to FGF10 expressing site. Scale bar: 200 μm. ( F , G ) The expression level of Anxa4 in cultured lung endoderm explants at 0 h, 24 h and 48 h. The expression level of Anxa4 was increased significantly at both 24 h and 48 h as compared to 0 h and its expression level was inhibited by MEK inhibitor, PD0325901 ( F ). The whole mount in situ hybridization of Anxa4 confirmed that PD0325901 treatment could decrease the expression of Anxa4 ( G ). Data are presented as mean ± SEM, n = 3; **p

    Article Snippet: For the Transwell® migration assays, 5 × 104 cells in 200 μl were seeded into the upper well of the Transwell® apparatus (6.5 mm diameter, 8 μm pore size, Corning Costar); 600 μl of DMEM/F12 medium supplemented with 400 ng/ml human recombinant FGF10 was added into the lower chamber.

    Techniques: Expressing, Cell Culture, Recombinant, Western Blot, Immunostaining, Staining, In Situ Hybridization