mouse anti his tag monoclonal antibody  (Sino Biological)


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    Name:
    Anti His tag Antibody Mouse Monoclonal
    Description:
    This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography
    Catalog Number:
    105327-mm02t
    Price:
    None
    Category:
    Primary Antibody
    Applications:
    WB
    Immunogen:
    A synthetic peptide corresponding to 6×His
    Antibody Type:
    MAb
    Host:
    Mouse
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    Structured Review

    Sino Biological mouse anti his tag monoclonal antibody
    This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography
    https://www.bioz.com/result/mouse anti his tag monoclonal antibody/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti his tag monoclonal antibody - by Bioz Stars, 2021-03
    94/100 stars

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    Related Articles

    Purification:

    Article Title: Functional Analysis of Odorant-Binding Proteins 12 and 17 from Wheat Blossom Midge Sitodiplosis mosellana Géhin (Diptera: Cecidomyiidae)
    Article Snippet: .. Solubilized SmosOBPs were purified using the Ni-NTA His·Bind Resin affinity column (7Sea Pharmatech Co., Shanghai, China) following the procedure described in Li et al. (2016) [ ], examined on 15% SDS-PAGE, and further verified by Western blot analysis using the mouse anti-His tag monoclonal antibody (Sino Biological, Beijing, China). .. Protein concentrations were determined by the Bicinchoninic Acid (BCA) Protein Assay Kit (GeneStar, Beijing, China).

    Article Title: Host Prdx6 contributing to the intracellular survival of Brucella suis S2 strain
    Article Snippet: As the 6 × His tag was fused to the N-terminus of the recombinant Prdx6 protein, an immobilized metal affinity chromatography method was utilized for rapid one-step purification employing an Ni-NTA column. .. The recombinant MmPrdx6 protein was purified and assessed via SDS-PAGE (Fig. b) and western blotting (Fig. c) using a commercial anti-His tag antibody (Sino Biological Inc.). .. The protein showed the expected molecular weight of approximately 28 kDa, including the molecular weight of the 6 × His-tag.

    Affinity Column:

    Article Title: Functional Analysis of Odorant-Binding Proteins 12 and 17 from Wheat Blossom Midge Sitodiplosis mosellana Géhin (Diptera: Cecidomyiidae)
    Article Snippet: .. Solubilized SmosOBPs were purified using the Ni-NTA His·Bind Resin affinity column (7Sea Pharmatech Co., Shanghai, China) following the procedure described in Li et al. (2016) [ ], examined on 15% SDS-PAGE, and further verified by Western blot analysis using the mouse anti-His tag monoclonal antibody (Sino Biological, Beijing, China). .. Protein concentrations were determined by the Bicinchoninic Acid (BCA) Protein Assay Kit (GeneStar, Beijing, China).

    SDS Page:

    Article Title: Functional Analysis of Odorant-Binding Proteins 12 and 17 from Wheat Blossom Midge Sitodiplosis mosellana Géhin (Diptera: Cecidomyiidae)
    Article Snippet: .. Solubilized SmosOBPs were purified using the Ni-NTA His·Bind Resin affinity column (7Sea Pharmatech Co., Shanghai, China) following the procedure described in Li et al. (2016) [ ], examined on 15% SDS-PAGE, and further verified by Western blot analysis using the mouse anti-His tag monoclonal antibody (Sino Biological, Beijing, China). .. Protein concentrations were determined by the Bicinchoninic Acid (BCA) Protein Assay Kit (GeneStar, Beijing, China).

    Article Title: Host Prdx6 contributing to the intracellular survival of Brucella suis S2 strain
    Article Snippet: As the 6 × His tag was fused to the N-terminus of the recombinant Prdx6 protein, an immobilized metal affinity chromatography method was utilized for rapid one-step purification employing an Ni-NTA column. .. The recombinant MmPrdx6 protein was purified and assessed via SDS-PAGE (Fig. b) and western blotting (Fig. c) using a commercial anti-His tag antibody (Sino Biological Inc.). .. The protein showed the expected molecular weight of approximately 28 kDa, including the molecular weight of the 6 × His-tag.

    Western Blot:

    Article Title: Functional Analysis of Odorant-Binding Proteins 12 and 17 from Wheat Blossom Midge Sitodiplosis mosellana Géhin (Diptera: Cecidomyiidae)
    Article Snippet: .. Solubilized SmosOBPs were purified using the Ni-NTA His·Bind Resin affinity column (7Sea Pharmatech Co., Shanghai, China) following the procedure described in Li et al. (2016) [ ], examined on 15% SDS-PAGE, and further verified by Western blot analysis using the mouse anti-His tag monoclonal antibody (Sino Biological, Beijing, China). .. Protein concentrations were determined by the Bicinchoninic Acid (BCA) Protein Assay Kit (GeneStar, Beijing, China).

    Article Title: Host Prdx6 contributing to the intracellular survival of Brucella suis S2 strain
    Article Snippet: As the 6 × His tag was fused to the N-terminus of the recombinant Prdx6 protein, an immobilized metal affinity chromatography method was utilized for rapid one-step purification employing an Ni-NTA column. .. The recombinant MmPrdx6 protein was purified and assessed via SDS-PAGE (Fig. b) and western blotting (Fig. c) using a commercial anti-His tag antibody (Sino Biological Inc.). .. The protein showed the expected molecular weight of approximately 28 kDa, including the molecular weight of the 6 × His-tag.

    Recombinant:

    Article Title: Host Prdx6 contributing to the intracellular survival of Brucella suis S2 strain
    Article Snippet: As the 6 × His tag was fused to the N-terminus of the recombinant Prdx6 protein, an immobilized metal affinity chromatography method was utilized for rapid one-step purification employing an Ni-NTA column. .. The recombinant MmPrdx6 protein was purified and assessed via SDS-PAGE (Fig. b) and western blotting (Fig. c) using a commercial anti-His tag antibody (Sino Biological Inc.). .. The protein showed the expected molecular weight of approximately 28 kDa, including the molecular weight of the 6 × His-tag.

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  • 94
    Sino Biological mouse anti his tag monoclonal antibody
    Mouse Anti His Tag Monoclonal Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti his tag monoclonal antibody/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti his tag monoclonal antibody - by Bioz Stars, 2021-03
    94/100 stars
      Buy from Supplier

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