mmp9  (Sino Biological)


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  • 93
    Name:
    MMP 9 Protein Human Recombinant
    Description:
    A DNA sequence encoding the pro form of human MMP9 enzyme NP 004985 2 Met 1 Asp 707 was expressed with a C terminal polyhistidine tag
    Catalog Number:
    10327-H08H
    Price:
    None
    Category:
    recombinant protein
    Product Aliases:
    CLG4B Protein Human, GELB Protein Human, MANDP2 Protein Human, MMP-9 Protein Human
    Host:
    HEK293 Cells
    Buy from Supplier


    Structured Review

    Sino Biological mmp9
    A DNA sequence encoding the pro form of human MMP9 enzyme NP 004985 2 Met 1 Asp 707 was expressed with a C terminal polyhistidine tag
    https://www.bioz.com/result/mmp9/product/Sino Biological
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mmp9 - by Bioz Stars, 2021-05
    93/100 stars

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    Related Articles

    Recombinant:

    Article Title: Comparison of Protein Immunoprecipitation-Multiple Reaction Monitoring with ELISA for Assay of Biomarker Candidates in Plasma
    Article Snippet: .. TIMP1, ENG and MMP9 recombinant proteins were purchase from Sino Biological (Beijing, China). .. THBS2, COMP and MSLN recombinant proteins were from R & D Systems.

    Article Title: MicroRNA-145 suppresses cell migration and invasion in upper tract urothelial carcinoma by targeting ARF6.
    Article Snippet: ADP-ribosylation factor 6 (ARF6) is a well-studied protein that is involved in multiple biological functions including cell migration and invasion. .. ADP-ribosylation factor 6 (ARF6) is a well-studied protein that is involved in multiple biological functions including cell migration and invasion. .. ADP-ribosylation factor 6 (ARF6) is a well-studied protein that is involved in multiple biological functions including cell migration and invasion.

    Article Title: Peptide Microarray-Based Metal Enhanced Fluorescence Assay for Multiple Profiling of Matrix Metalloproteinases Activities.
    Article Snippet: Matrix metalloproteinases (MMPs) are closely associated with cancer cell invasion and metastasis. .. Matrix metalloproteinases (MMPs) are closely associated with cancer cell invasion and metastasis. .. Matrix metalloproteinases (MMPs) are closely associated with cancer cell invasion and metastasis.

    Article Title: Hairy/enhancer-of-split related with YRPW motif protein 1 promotes osteosarcoma metastasis via matrix metallopeptidase 9 expression
    Article Snippet: To confirm whether HEY1 induce a cell invasion, we performed an invasion assay which equalises the number of each osteosarcoma cell line. .. To determine whether MMP9 rescued the decrease in the invasive activity, we added the recombinant active human MMP9 (Sino Biological Inc., Peking, China) to the upper chamber. .. Western blot Cells were lysed by using NP40 lysis buffer (0.5% NP40, 10 mM Tris-HCl (pH 7.4), 150 mM NaCl, 3 mM pAPMSF (Wako Chemicals, Kanagawa, Japan), 5 mg ml−1 aprotinin (Sigma, St. Louis, MO, USA), 2 mM sodium orthovanadate (Wako Chemicals) and 5 mM EDTA.

    Article Title: Evaluation of Matrix Metalloproteinase Inhibition by Peptide Microarray-Based Fluorescence Assay on Polymer Brush Substrate and in Vivo Assessment.
    Article Snippet: .. Matrix metalloproteinases (MMPs) are important biomarkers and potential therapeutic targets of tumor. .. Matrix metalloproteinases (MMPs) are important biomarkers and potential therapeutic targets of tumor.

    Positive Control:

    Article Title: MicroRNA-145 suppresses cell migration and invasion in upper tract urothelial carcinoma by targeting ARF6.
    Article Snippet: ADP-ribosylation factor 6 (ARF6) is a well-studied protein that is involved in multiple biological functions including cell migration and invasion. .. ADP-ribosylation factor 6 (ARF6) is a well-studied protein that is involved in multiple biological functions including cell migration and invasion. .. ADP-ribosylation factor 6 (ARF6) is a well-studied protein that is involved in multiple biological functions including cell migration and invasion.

    Activity Assay:

    Article Title: Hairy/enhancer-of-split related with YRPW motif protein 1 promotes osteosarcoma metastasis via matrix metallopeptidase 9 expression
    Article Snippet: To confirm whether HEY1 induce a cell invasion, we performed an invasion assay which equalises the number of each osteosarcoma cell line. .. To determine whether MMP9 rescued the decrease in the invasive activity, we added the recombinant active human MMP9 (Sino Biological Inc., Peking, China) to the upper chamber. .. Western blot Cells were lysed by using NP40 lysis buffer (0.5% NP40, 10 mM Tris-HCl (pH 7.4), 150 mM NaCl, 3 mM pAPMSF (Wako Chemicals, Kanagawa, Japan), 5 mg ml−1 aprotinin (Sigma, St. Louis, MO, USA), 2 mM sodium orthovanadate (Wako Chemicals) and 5 mM EDTA.

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  • 90
    Sino Biological anti mmp9
    ( a ) Diagram of a direct immunoassay and ( b ) recognition of <t>MMP9:</t> wavenumber shift monitored in function of concentration and reflectivity.
    Anti Mmp9, supplied by Sino Biological, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti mmp9/product/Sino Biological
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti mmp9 - by Bioz Stars, 2021-05
    90/100 stars
      Buy from Supplier

    93
    Sino Biological recombinant active human mmp9
    Knockdown of HEY1 decreased the expression of <t>MMP9</t> in human osteosarcoma cells. MMP9 expression levels in 143B, HS-Os-1, MG63 and NOS1 cells were assessed by using real-time PCR 24 h after transfection with control siRNA or HEY1 siRNA. The experiment was performed in triplicate with similar results. * P
    Recombinant Active Human Mmp9, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant active human mmp9/product/Sino Biological
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant active human mmp9 - by Bioz Stars, 2021-05
    93/100 stars
      Buy from Supplier

    Image Search Results


    ( a ) Diagram of a direct immunoassay and ( b ) recognition of MMP9: wavenumber shift monitored in function of concentration and reflectivity.

    Journal: Sensors (Basel, Switzerland)

    Article Title: Development towards Compact Nitrocellulose-Based Interferometric Biochips for Dry Eye MMP9 Label-Free In-Situ Diagnosis

    doi: 10.3390/s17051158

    Figure Lengend Snippet: ( a ) Diagram of a direct immunoassay and ( b ) recognition of MMP9: wavenumber shift monitored in function of concentration and reflectivity.

    Article Snippet: Then, an incubation of anti-MMP9 (Sino Biological Inc.) antibody is carried out to properly immobilize and orientate the anti-MMP9 onto this sensing surface.

    Techniques: Concentration Assay

    ( a ) Raw signal of MMP9 in contrived tears; ( b ) MMP9 signal in contrived tears subtracting the matrix effect.

    Journal: Sensors (Basel, Switzerland)

    Article Title: Development towards Compact Nitrocellulose-Based Interferometric Biochips for Dry Eye MMP9 Label-Free In-Situ Diagnosis

    doi: 10.3390/s17051158

    Figure Lengend Snippet: ( a ) Raw signal of MMP9 in contrived tears; ( b ) MMP9 signal in contrived tears subtracting the matrix effect.

    Article Snippet: Then, an incubation of anti-MMP9 (Sino Biological Inc.) antibody is carried out to properly immobilize and orientate the anti-MMP9 onto this sensing surface.

    Techniques:

    MMP9 signal in real tear samples.

    Journal: Sensors (Basel, Switzerland)

    Article Title: Development towards Compact Nitrocellulose-Based Interferometric Biochips for Dry Eye MMP9 Label-Free In-Situ Diagnosis

    doi: 10.3390/s17051158

    Figure Lengend Snippet: MMP9 signal in real tear samples.

    Article Snippet: Then, an incubation of anti-MMP9 (Sino Biological Inc.) antibody is carried out to properly immobilize and orientate the anti-MMP9 onto this sensing surface.

    Techniques:

    Knockdown of HEY1 decreased the expression of MMP9 in human osteosarcoma cells. MMP9 expression levels in 143B, HS-Os-1, MG63 and NOS1 cells were assessed by using real-time PCR 24 h after transfection with control siRNA or HEY1 siRNA. The experiment was performed in triplicate with similar results. * P

    Journal: British Journal of Cancer

    Article Title: Hairy/enhancer-of-split related with YRPW motif protein 1 promotes osteosarcoma metastasis via matrix metallopeptidase 9 expression

    doi: 10.1038/bjc.2015.84

    Figure Lengend Snippet: Knockdown of HEY1 decreased the expression of MMP9 in human osteosarcoma cells. MMP9 expression levels in 143B, HS-Os-1, MG63 and NOS1 cells were assessed by using real-time PCR 24 h after transfection with control siRNA or HEY1 siRNA. The experiment was performed in triplicate with similar results. * P

    Article Snippet: To determine whether MMP9 rescued the decrease in the invasive activity, we added the recombinant active human MMP9 (Sino Biological Inc., Peking, China) to the upper chamber.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Transfection

    Strong association between HEY1 and MMP9 expression. We examined the expression of HEY1 and MMP9. HEY1 and MMP9 mRNA expression was examined by using NHost (normal osteoblast), UBE6T15 (mesenchymal stem cell), and eight human osteosarcoma cell lines. Spearman's rank correlation coefficient test showed that correlation between HEY1 and MMP9 expression was significant. The experiment was performed in triplicate with similar results. P

    Journal: British Journal of Cancer

    Article Title: Hairy/enhancer-of-split related with YRPW motif protein 1 promotes osteosarcoma metastasis via matrix metallopeptidase 9 expression

    doi: 10.1038/bjc.2015.84

    Figure Lengend Snippet: Strong association between HEY1 and MMP9 expression. We examined the expression of HEY1 and MMP9. HEY1 and MMP9 mRNA expression was examined by using NHost (normal osteoblast), UBE6T15 (mesenchymal stem cell), and eight human osteosarcoma cell lines. Spearman's rank correlation coefficient test showed that correlation between HEY1 and MMP9 expression was significant. The experiment was performed in triplicate with similar results. P

    Article Snippet: To determine whether MMP9 rescued the decrease in the invasive activity, we added the recombinant active human MMP9 (Sino Biological Inc., Peking, China) to the upper chamber.

    Techniques: Expressing

    HEY1 -regulated osteosarcoma invasion is mediated by MMP9. ( A ) ELISA assay was performed to examine the expression of MMP9 following control siRNA or HEY1 siRNA transfection. ( B ) Control siRNA/143B or HEY1 siRNA/143B transfected cells were examined by using the invasion assay. The number of invasive cells was decreased in HEY1 shRNA-expressing cells. To determine if a decrease of invasion due to HEY1 knockdown could be reversed by the addition of active MMP9 protein or not, the invasion assay was performed with and without active MMP9. The experiment was performed in triplicate with similar results. * P

    Journal: British Journal of Cancer

    Article Title: Hairy/enhancer-of-split related with YRPW motif protein 1 promotes osteosarcoma metastasis via matrix metallopeptidase 9 expression

    doi: 10.1038/bjc.2015.84

    Figure Lengend Snippet: HEY1 -regulated osteosarcoma invasion is mediated by MMP9. ( A ) ELISA assay was performed to examine the expression of MMP9 following control siRNA or HEY1 siRNA transfection. ( B ) Control siRNA/143B or HEY1 siRNA/143B transfected cells were examined by using the invasion assay. The number of invasive cells was decreased in HEY1 shRNA-expressing cells. To determine if a decrease of invasion due to HEY1 knockdown could be reversed by the addition of active MMP9 protein or not, the invasion assay was performed with and without active MMP9. The experiment was performed in triplicate with similar results. * P

    Article Snippet: To determine whether MMP9 rescued the decrease in the invasive activity, we added the recombinant active human MMP9 (Sino Biological Inc., Peking, China) to the upper chamber.

    Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Transfection, Invasion Assay, shRNA