Journal: Archives of Toxicology

Article Title: In vitro evaluation of the reductive carbonyl idarubicin metabolism to evaluate inhibitors of the formation of cardiotoxic idarubicinol via carbonyl and aldo–keto reductases

doi: 10.1007/s00204-023-03661-7

Figure Lengend Snippet: Western blot of the AKR1B1, AKR1A1, AKR1C3, CBR1, and CBR3 in HepG2, MCF-7, and HEK293 cells. β-actin served as a loading control. Depicted is one blot of a series of three for each protein investigated

Article Snippet: The mouse monoclonal antibodies against β-actin (sc-47778), AKR1B1 (aldose reductase (H-6, sc-166918)), CBR1 (B-11, sc-390554), and CBR3 (E-12, sc-374393) were obtained from Santa Cruz (Heidelberg, Germany), the rabbit monoclonal antibody against AKR1C3 (ab209899) and the rabbit polyclonal antibody against AKR1A1 (ab125878) were from Abcam (Cambridge, United Kingdom).

Techniques: Western Blot, Control

Journal: Archives of Toxicology

Article Title: In vitro evaluation of the reductive carbonyl idarubicin metabolism to evaluate inhibitors of the formation of cardiotoxic idarubicinol via carbonyl and aldo–keto reductases

doi: 10.1007/s00204-023-03661-7

Figure Lengend Snippet: Inhibition of idarubicinol formation by different inhibitors in HEK293, HepG2, and MCF-7 cells

Article Snippet: The mouse monoclonal antibodies against β-actin (sc-47778), AKR1B1 (aldose reductase (H-6, sc-166918)), CBR1 (B-11, sc-390554), and CBR3 (E-12, sc-374393) were obtained from Santa Cruz (Heidelberg, Germany), the rabbit monoclonal antibody against AKR1C3 (ab209899) and the rabbit polyclonal antibody against AKR1A1 (ab125878) were from Abcam (Cambridge, United Kingdom).

Techniques: Inhibition

Journal: Archives of Toxicology

Article Title: In vitro evaluation of the reductive carbonyl idarubicin metabolism to evaluate inhibitors of the formation of cardiotoxic idarubicinol via carbonyl and aldo–keto reductases

doi: 10.1007/s00204-023-03661-7

Figure Lengend Snippet: Expression of the mRNA of the five reductases in human liver samples and HepG2 cells. mRNA expression of AKR1A1, AKR1B1, AKR1C3, CBR1 , and CBR3 was quantified in 7 human liver samples and HepG2 cells via real-time RT-PCR and normalized to the expression of the two housekeeping genes HUPO and RPL13 . Each data point for the liver samples represents the mean of a technical duplicate and the data for the HepG2 cells represent the mean ± S.E.M. for n = 7

Article Snippet: The mouse monoclonal antibodies against β-actin (sc-47778), AKR1B1 (aldose reductase (H-6, sc-166918)), CBR1 (B-11, sc-390554), and CBR3 (E-12, sc-374393) were obtained from Santa Cruz (Heidelberg, Germany), the rabbit monoclonal antibody against AKR1C3 (ab209899) and the rabbit polyclonal antibody against AKR1A1 (ab125878) were from Abcam (Cambridge, United Kingdom).

Techniques: Expressing, Quantitative RT-PCR

Journal: Toxicology in vitro : an international journal published in association with BIBRA

Article Title: Effects of cellular differentiation in human primary bronchial epithelial cells: Metabolism of 4-(methylnitrosamine)-1-(3-pyridyl)-1-butanone

doi: 10.1016/j.tiv.2018.12.006

Figure Lengend Snippet: Primers for the qRT-PCR.

Article Snippet: Rabbit anti-carbonyl reductase 1 antibody and mouse anti-GAPDH were purchased from Santa Cruz Biotechnology (Dallas, TX).

Techniques: Amplification

Journal: Toxicology in vitro : an international journal published in association with BIBRA

Article Title: Effects of cellular differentiation in human primary bronchial epithelial cells: Metabolism of 4-(methylnitrosamine)-1-(3-pyridyl)-1-butanone

doi: 10.1016/j.tiv.2018.12.006

Figure Lengend Snippet: Protein expression of the major metabolic enzymes implicated in NNK metabolism. Protein expressions of CYP2A6/2A13 and carbonyl reductase 1 were analyzed using immunoblotting in NHBE cells and ALI cultures. β-Actin was used as the loading control.

Article Snippet: Rabbit anti-carbonyl reductase 1 antibody and mouse anti-GAPDH were purchased from Santa Cruz Biotechnology (Dallas, TX).

Techniques: Expressing, Western Blot