mers cov s glycoprotein  (Sino Biological)


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    Name:
    MERS CoV Spike Protein S2 Antibody Rabbit PAb
    Description:
    Produced in rabbits immunized with purified a synthetic peptide corresponding to the S2 subunit of MERS CoV NCoV Novel coronavirus spike glycoprotein S protein and purified by antigen affinity chromatography
    Catalog Number:
    100210-RP02
    Price:
    None
    Category:
    Primary Antibody
    Reactivity:
    MERS CoV
    Applications:
    WB
    Immunogen:
    A synthetic peptide corresponding to the S2 subunit of MERS-CoV (NCoV / Novel coronavirus) spike glycoprotein (S protein).
    Product Aliases:
    Anti-coronavirus s1 Antibody, Anti-coronavirus s2 Antibody, Anti-coronavirus spike Antibody, Anti-cov spike Antibody, Anti-ncov RBD Antibody, Anti-ncov s1 Antibody, Anti-ncov s2 Antibody, Anti-ncov spike Antibody, Anti-RBD Antibody, Anti-S Antibody, Anti-s1 Antibody, Anti-Spike RBD Antibody
    Antibody Type:
    PAb
    Host:
    Rabbit
    Isotype:
    Rabbit IgG
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    Structured Review

    Sino Biological mers cov s glycoprotein
    The increase in the concentration of IFN-γ in the media of splenocytes from immunized mice after re-stimulation with the recombinant full-length <t>MERS-CoV</t> S protein. Scatter plots show the median (95% CI) increase (fold change) in IFN-γ production following re-stimulation for each group from one representative experiment (n = 3 mice/group). Asterisks indicate significant differences in IFN-γ production between the cells taken from vaccinated and intact animals. * p
    Produced in rabbits immunized with purified a synthetic peptide corresponding to the S2 subunit of MERS CoV NCoV Novel coronavirus spike glycoprotein S protein and purified by antigen affinity chromatography
    https://www.bioz.com/result/mers cov s glycoprotein/product/Sino Biological
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mers cov s glycoprotein - by Bioz Stars, 2021-04
    93/100 stars

    Images

    1) Product Images from "Immunogenicity of Different Forms of Middle East Respiratory Syndrome S Glycoprotein"

    Article Title: Immunogenicity of Different Forms of Middle East Respiratory Syndrome S Glycoprotein

    Journal: Acta Naturae

    doi:

    The increase in the concentration of IFN-γ in the media of splenocytes from immunized mice after re-stimulation with the recombinant full-length MERS-CoV S protein. Scatter plots show the median (95% CI) increase (fold change) in IFN-γ production following re-stimulation for each group from one representative experiment (n = 3 mice/group). Asterisks indicate significant differences in IFN-γ production between the cells taken from vaccinated and intact animals. * p
    Figure Legend Snippet: The increase in the concentration of IFN-γ in the media of splenocytes from immunized mice after re-stimulation with the recombinant full-length MERS-CoV S protein. Scatter plots show the median (95% CI) increase (fold change) in IFN-γ production following re-stimulation for each group from one representative experiment (n = 3 mice/group). Asterisks indicate significant differences in IFN-γ production between the cells taken from vaccinated and intact animals. * p

    Techniques Used: Concentration Assay, Mouse Assay, Recombinant

    A – Schematic structures of target transgenes in the rAd5-S, rAd5-S-G, rAd5-RBD, rAd5-RBD-G, and rAd5-RBD-Fc genomes. Cmv – a promoter of the E1 region of human cytomegalovirus; G – the gene of the G glycoprotein of the vesicular stomatitis virus; LP – the leader-peptide sequence directing protein secretion; pA – polyadenylation signal; RBD – the receptor-binding domain of MERS-CoV S glycoprotein; Spike – MERS CoV S glycoprotein. B – Western blot analysis of the MERS-CoV S glycoprotein variants expressed by each rAd5. Lane 1 – lysate from control Ad5-null cells; lane 2 – intact cells; lane 3 – lysate from rAd5-S cells; lane 4 – lysate from rAd5-S-G cells; lane 5 – the medium from the rAd5- RBD cell culture; lane 6 – lysate from rAd5-RBD-G cells; lane 7 – the medium from the rAd5-RBD-Fc cell culture
    Figure Legend Snippet: A – Schematic structures of target transgenes in the rAd5-S, rAd5-S-G, rAd5-RBD, rAd5-RBD-G, and rAd5-RBD-Fc genomes. Cmv – a promoter of the E1 region of human cytomegalovirus; G – the gene of the G glycoprotein of the vesicular stomatitis virus; LP – the leader-peptide sequence directing protein secretion; pA – polyadenylation signal; RBD – the receptor-binding domain of MERS-CoV S glycoprotein; Spike – MERS CoV S glycoprotein. B – Western blot analysis of the MERS-CoV S glycoprotein variants expressed by each rAd5. Lane 1 – lysate from control Ad5-null cells; lane 2 – intact cells; lane 3 – lysate from rAd5-S cells; lane 4 – lysate from rAd5-S-G cells; lane 5 – the medium from the rAd5- RBD cell culture; lane 6 – lysate from rAd5-RBD-G cells; lane 7 – the medium from the rAd5-RBD-Fc cell culture

    Techniques Used: Sequencing, Binding Assay, Western Blot, Cell Culture

    Glycoprotein-specific IgG titers in the blood serum of immunized animals. The figure shows IgG titers: ( A ) specific to the MERS-CoV S glycoprotein and ( B ) specific to the RBD. Scatter plots show the geometric mean titer (GMT) and 95% confidence interval (CI) for each group (n = 5 mice/group). Asterisks indicate significant intergroup differences in IgG titers. * p
    Figure Legend Snippet: Glycoprotein-specific IgG titers in the blood serum of immunized animals. The figure shows IgG titers: ( A ) specific to the MERS-CoV S glycoprotein and ( B ) specific to the RBD. Scatter plots show the geometric mean titer (GMT) and 95% confidence interval (CI) for each group (n = 5 mice/group). Asterisks indicate significant intergroup differences in IgG titers. * p

    Techniques Used: Mouse Assay

    Analysis of IgG antibody isotypes in mice after immunization with rAd5 expressing different forms of the MERS-CoV S glycoprotein. The figure shows the titers of IgG1 ( A ), IgG2a ( B ), IgG2b ( C ), and IgG3 ( D ) specific to the MERS-CoV S glycoprotein in the serum samples of immunized animals. Scatter plots show the geometric mean titer (GMT) and 95% confidence interval (CI) for each group (n = 5). Asterisks indicate significant intergroup differences in IgG titers. * p
    Figure Legend Snippet: Analysis of IgG antibody isotypes in mice after immunization with rAd5 expressing different forms of the MERS-CoV S glycoprotein. The figure shows the titers of IgG1 ( A ), IgG2a ( B ), IgG2b ( C ), and IgG3 ( D ) specific to the MERS-CoV S glycoprotein in the serum samples of immunized animals. Scatter plots show the geometric mean titer (GMT) and 95% confidence interval (CI) for each group (n = 5). Asterisks indicate significant intergroup differences in IgG titers. * p

    Techniques Used: Mouse Assay, Expressing

    The titers of neutralizing antibodies in the blood serum of immunized animals. The neutralization assay was performed using lentiviral particles pseudotyped with MERS-CoV S glycoprotein. Scatter plots show the geometric mean titer (GMT) and 95% confidence interval (CI) for each group (n = 5). Asterisks indicate no significant intergroup differences in the titers of neutralizing antibodies. * p > 0.05, Mann–Whitney U test
    Figure Legend Snippet: The titers of neutralizing antibodies in the blood serum of immunized animals. The neutralization assay was performed using lentiviral particles pseudotyped with MERS-CoV S glycoprotein. Scatter plots show the geometric mean titer (GMT) and 95% confidence interval (CI) for each group (n = 5). Asterisks indicate no significant intergroup differences in the titers of neutralizing antibodies. * p > 0.05, Mann–Whitney U test

    Techniques Used: Neutralization, MANN-WHITNEY

    The study of lymphoproliferative activity of splenocytes in immunized mice. The figure shows the levels (%) of proliferating CD4+ and CD8+T cells re-stimulated by the MERS-CoV S protein on day 8 post-vaccination. Scatter plots show the median lymphoproliferative activity of re-stimulated cells (%) with 95% CIs for each group from one representative experiment (n = 6 mice/group). Asterisks indicate significant differences in the percentage of proliferating cells between vaccinated and intact animals. * p
    Figure Legend Snippet: The study of lymphoproliferative activity of splenocytes in immunized mice. The figure shows the levels (%) of proliferating CD4+ and CD8+T cells re-stimulated by the MERS-CoV S protein on day 8 post-vaccination. Scatter plots show the median lymphoproliferative activity of re-stimulated cells (%) with 95% CIs for each group from one representative experiment (n = 6 mice/group). Asterisks indicate significant differences in the percentage of proliferating cells between vaccinated and intact animals. * p

    Techniques Used: Activity Assay, Mouse Assay

    Related Articles

    Incubation:

    Article Title: A novel peptide with potent and broad-spectrum antiviral activities against multiple respiratory viruses
    Article Snippet: The transferred membrane was incubated with P9 (2 μg/ml) at room temperature for 1 h, followed by incubation with rabbit anti-smBD4 (1:5000) for 1 h to detect P9 binding. .. For detection of HA, NA, S1, and S2, the membrane was incubated with rabbit-anti-HA1 (1:4,000, Immune Technology Crop., IT-003-001), or rabbit-anti-NA (1:4,000, Immune Technology Crop., IT-003-038), rabbit-anti-S1, (1:4000, Sino Biological Inc. 100209-RP02) or rabbit-anti-S2 (1:4000, Sino Biological Inc. 100210-RP02) at room temperature for 1 h. Goat anti-rabbit IgG-HRP (1:10,000, Invitrogen, 656120) was used as the secondary antibody to detect the binding at room temperature for 1 h. After the membrane was probed with the indicated antibodies or the peptide, immunoreactive bands were visualized by enhanced chemiluminescence. .. ELISA Binding affinities of the peptides to viral protein were detected by ELISA as described previously with some modification.

    Binding Assay:

    Article Title: A novel peptide with potent and broad-spectrum antiviral activities against multiple respiratory viruses
    Article Snippet: The transferred membrane was incubated with P9 (2 μg/ml) at room temperature for 1 h, followed by incubation with rabbit anti-smBD4 (1:5000) for 1 h to detect P9 binding. .. For detection of HA, NA, S1, and S2, the membrane was incubated with rabbit-anti-HA1 (1:4,000, Immune Technology Crop., IT-003-001), or rabbit-anti-NA (1:4,000, Immune Technology Crop., IT-003-038), rabbit-anti-S1, (1:4000, Sino Biological Inc. 100209-RP02) or rabbit-anti-S2 (1:4000, Sino Biological Inc. 100210-RP02) at room temperature for 1 h. Goat anti-rabbit IgG-HRP (1:10,000, Invitrogen, 656120) was used as the secondary antibody to detect the binding at room temperature for 1 h. After the membrane was probed with the indicated antibodies or the peptide, immunoreactive bands were visualized by enhanced chemiluminescence. .. ELISA Binding affinities of the peptides to viral protein were detected by ELISA as described previously with some modification.

    Article Title: Highly sensitive and specific multiplex antibody assays to quantify immunoglobulins M, A and G against SARS-CoV-2 antigens
    Article Snippet: .. Antigens The Receptor-Binding Domain (RBD) of the spike (S) glycoprotein of SARS-CoV-2, the leading vaccine candidate target, was selected as the primary antigen to develop the initial qSAT assay because (i) S is one of the most immunogenic surface proteins together with the nucleocapsid protein (N) ( ) (ii) RBD is the fragment of the virus that mediates binding to the host receptor ACE2 in the lung cells ( ) (iii) antibodies to RBD correlate with neutralizing antibodies ( )( ) that could be associated with protection based on studies of other coronaviruses and animal models ( – ), and (iv) an ELISA based on this same protein has received FDA approval for COVID-19 serology ( ). .. The RBD was from the Krammer lab (Mount Sinai, New York, USA) ( ) and the S antigen was produced in-house using Chinese Hamster Ovary (CHO) cells transiently-transfected with the Krammer plasmid followed by purification of the recombinant protein from 4-day culture supernatants using nickel affinity chromatography.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Highly sensitive and specific multiplex antibody assays to quantify immunoglobulins M, A and G against SARS-CoV-2 antigens
    Article Snippet: .. Antigens The Receptor-Binding Domain (RBD) of the spike (S) glycoprotein of SARS-CoV-2, the leading vaccine candidate target, was selected as the primary antigen to develop the initial qSAT assay because (i) S is one of the most immunogenic surface proteins together with the nucleocapsid protein (N) ( ) (ii) RBD is the fragment of the virus that mediates binding to the host receptor ACE2 in the lung cells ( ) (iii) antibodies to RBD correlate with neutralizing antibodies ( )( ) that could be associated with protection based on studies of other coronaviruses and animal models ( – ), and (iv) an ELISA based on this same protein has received FDA approval for COVID-19 serology ( ). .. The RBD was from the Krammer lab (Mount Sinai, New York, USA) ( ) and the S antigen was produced in-house using Chinese Hamster Ovary (CHO) cells transiently-transfected with the Krammer plasmid followed by purification of the recombinant protein from 4-day culture supernatants using nickel affinity chromatography.

    Sequencing:

    Article Title: Immunogenicity of Different Forms of Middle East Respiratory Syndrome S Glycoprotein
    Article Snippet: Construction of recombinant adenoviral particles expressing different forms of the MERS-CoV S glycoproteinThe MERS-CoV S glycoprotein amino acid sequences of strains (2015–2017) were obtained from the NCBI database [ ]. .. The consensus sequence of the MERS-CoV S glycoprotein was made on the basis of amino acid sequences using the Geneious® 10.2.3 software. .. The nucleotide sequences of different glycoprotein forms were optimized for expression in mammalian cell lines and synthesized by Evrogen JSC (Russia).

    Software:

    Article Title: Immunogenicity of Different Forms of Middle East Respiratory Syndrome S Glycoprotein
    Article Snippet: Construction of recombinant adenoviral particles expressing different forms of the MERS-CoV S glycoproteinThe MERS-CoV S glycoprotein amino acid sequences of strains (2015–2017) were obtained from the NCBI database [ ]. .. The consensus sequence of the MERS-CoV S glycoprotein was made on the basis of amino acid sequences using the Geneious® 10.2.3 software. .. The nucleotide sequences of different glycoprotein forms were optimized for expression in mammalian cell lines and synthesized by Evrogen JSC (Russia).

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  • 99
    Sino Biological mers cov s glycoprotein
    The increase in the concentration of IFN-γ in the media of splenocytes from immunized mice after re-stimulation with the recombinant full-length <t>MERS-CoV</t> S protein. Scatter plots show the median (95% CI) increase (fold change) in IFN-γ production following re-stimulation for each group from one representative experiment (n = 3 mice/group). Asterisks indicate significant differences in IFN-γ production between the cells taken from vaccinated and intact animals. * p
    Mers Cov S Glycoprotein, supplied by Sino Biological, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mers cov s glycoprotein/product/Sino Biological
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mers cov s glycoprotein - by Bioz Stars, 2021-04
    99/100 stars
      Buy from Supplier

    93
    Sino Biological mers cov spike protein s2 antibody rabbit pab
    The increase in the concentration of IFN-γ in the media of splenocytes from immunized mice after re-stimulation with the recombinant full-length <t>MERS-CoV</t> S protein. Scatter plots show the median (95% CI) increase (fold change) in IFN-γ production following re-stimulation for each group from one representative experiment (n = 3 mice/group). Asterisks indicate significant differences in IFN-γ production between the cells taken from vaccinated and intact animals. * p
    Mers Cov Spike Protein S2 Antibody Rabbit Pab, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mers cov spike protein s2 antibody rabbit pab/product/Sino Biological
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mers cov spike protein s2 antibody rabbit pab - by Bioz Stars, 2021-04
    93/100 stars
      Buy from Supplier

    Image Search Results


    The increase in the concentration of IFN-γ in the media of splenocytes from immunized mice after re-stimulation with the recombinant full-length MERS-CoV S protein. Scatter plots show the median (95% CI) increase (fold change) in IFN-γ production following re-stimulation for each group from one representative experiment (n = 3 mice/group). Asterisks indicate significant differences in IFN-γ production between the cells taken from vaccinated and intact animals. * p

    Journal: Acta Naturae

    Article Title: Immunogenicity of Different Forms of Middle East Respiratory Syndrome S Glycoprotein

    doi:

    Figure Lengend Snippet: The increase in the concentration of IFN-γ in the media of splenocytes from immunized mice after re-stimulation with the recombinant full-length MERS-CoV S protein. Scatter plots show the median (95% CI) increase (fold change) in IFN-γ production following re-stimulation for each group from one representative experiment (n = 3 mice/group). Asterisks indicate significant differences in IFN-γ production between the cells taken from vaccinated and intact animals. * p

    Article Snippet: Evaluating the expression of different forms of the MERS-CoV S glycoprotein by western blottingHEK293 cells were seeded in 35 mm Petri dishes and incubated overnight to 70% confluence.

    Techniques: Concentration Assay, Mouse Assay, Recombinant

    A – Schematic structures of target transgenes in the rAd5-S, rAd5-S-G, rAd5-RBD, rAd5-RBD-G, and rAd5-RBD-Fc genomes. Cmv – a promoter of the E1 region of human cytomegalovirus; G – the gene of the G glycoprotein of the vesicular stomatitis virus; LP – the leader-peptide sequence directing protein secretion; pA – polyadenylation signal; RBD – the receptor-binding domain of MERS-CoV S glycoprotein; Spike – MERS CoV S glycoprotein. B – Western blot analysis of the MERS-CoV S glycoprotein variants expressed by each rAd5. Lane 1 – lysate from control Ad5-null cells; lane 2 – intact cells; lane 3 – lysate from rAd5-S cells; lane 4 – lysate from rAd5-S-G cells; lane 5 – the medium from the rAd5- RBD cell culture; lane 6 – lysate from rAd5-RBD-G cells; lane 7 – the medium from the rAd5-RBD-Fc cell culture

    Journal: Acta Naturae

    Article Title: Immunogenicity of Different Forms of Middle East Respiratory Syndrome S Glycoprotein

    doi:

    Figure Lengend Snippet: A – Schematic structures of target transgenes in the rAd5-S, rAd5-S-G, rAd5-RBD, rAd5-RBD-G, and rAd5-RBD-Fc genomes. Cmv – a promoter of the E1 region of human cytomegalovirus; G – the gene of the G glycoprotein of the vesicular stomatitis virus; LP – the leader-peptide sequence directing protein secretion; pA – polyadenylation signal; RBD – the receptor-binding domain of MERS-CoV S glycoprotein; Spike – MERS CoV S glycoprotein. B – Western blot analysis of the MERS-CoV S glycoprotein variants expressed by each rAd5. Lane 1 – lysate from control Ad5-null cells; lane 2 – intact cells; lane 3 – lysate from rAd5-S cells; lane 4 – lysate from rAd5-S-G cells; lane 5 – the medium from the rAd5- RBD cell culture; lane 6 – lysate from rAd5-RBD-G cells; lane 7 – the medium from the rAd5-RBD-Fc cell culture

    Article Snippet: Evaluating the expression of different forms of the MERS-CoV S glycoprotein by western blottingHEK293 cells were seeded in 35 mm Petri dishes and incubated overnight to 70% confluence.

    Techniques: Sequencing, Binding Assay, Western Blot, Cell Culture

    Glycoprotein-specific IgG titers in the blood serum of immunized animals. The figure shows IgG titers: ( A ) specific to the MERS-CoV S glycoprotein and ( B ) specific to the RBD. Scatter plots show the geometric mean titer (GMT) and 95% confidence interval (CI) for each group (n = 5 mice/group). Asterisks indicate significant intergroup differences in IgG titers. * p

    Journal: Acta Naturae

    Article Title: Immunogenicity of Different Forms of Middle East Respiratory Syndrome S Glycoprotein

    doi:

    Figure Lengend Snippet: Glycoprotein-specific IgG titers in the blood serum of immunized animals. The figure shows IgG titers: ( A ) specific to the MERS-CoV S glycoprotein and ( B ) specific to the RBD. Scatter plots show the geometric mean titer (GMT) and 95% confidence interval (CI) for each group (n = 5 mice/group). Asterisks indicate significant intergroup differences in IgG titers. * p

    Article Snippet: Evaluating the expression of different forms of the MERS-CoV S glycoprotein by western blottingHEK293 cells were seeded in 35 mm Petri dishes and incubated overnight to 70% confluence.

    Techniques: Mouse Assay

    Analysis of IgG antibody isotypes in mice after immunization with rAd5 expressing different forms of the MERS-CoV S glycoprotein. The figure shows the titers of IgG1 ( A ), IgG2a ( B ), IgG2b ( C ), and IgG3 ( D ) specific to the MERS-CoV S glycoprotein in the serum samples of immunized animals. Scatter plots show the geometric mean titer (GMT) and 95% confidence interval (CI) for each group (n = 5). Asterisks indicate significant intergroup differences in IgG titers. * p

    Journal: Acta Naturae

    Article Title: Immunogenicity of Different Forms of Middle East Respiratory Syndrome S Glycoprotein

    doi:

    Figure Lengend Snippet: Analysis of IgG antibody isotypes in mice after immunization with rAd5 expressing different forms of the MERS-CoV S glycoprotein. The figure shows the titers of IgG1 ( A ), IgG2a ( B ), IgG2b ( C ), and IgG3 ( D ) specific to the MERS-CoV S glycoprotein in the serum samples of immunized animals. Scatter plots show the geometric mean titer (GMT) and 95% confidence interval (CI) for each group (n = 5). Asterisks indicate significant intergroup differences in IgG titers. * p

    Article Snippet: Evaluating the expression of different forms of the MERS-CoV S glycoprotein by western blottingHEK293 cells were seeded in 35 mm Petri dishes and incubated overnight to 70% confluence.

    Techniques: Mouse Assay, Expressing

    The titers of neutralizing antibodies in the blood serum of immunized animals. The neutralization assay was performed using lentiviral particles pseudotyped with MERS-CoV S glycoprotein. Scatter plots show the geometric mean titer (GMT) and 95% confidence interval (CI) for each group (n = 5). Asterisks indicate no significant intergroup differences in the titers of neutralizing antibodies. * p > 0.05, Mann–Whitney U test

    Journal: Acta Naturae

    Article Title: Immunogenicity of Different Forms of Middle East Respiratory Syndrome S Glycoprotein

    doi:

    Figure Lengend Snippet: The titers of neutralizing antibodies in the blood serum of immunized animals. The neutralization assay was performed using lentiviral particles pseudotyped with MERS-CoV S glycoprotein. Scatter plots show the geometric mean titer (GMT) and 95% confidence interval (CI) for each group (n = 5). Asterisks indicate no significant intergroup differences in the titers of neutralizing antibodies. * p > 0.05, Mann–Whitney U test

    Article Snippet: Evaluating the expression of different forms of the MERS-CoV S glycoprotein by western blottingHEK293 cells were seeded in 35 mm Petri dishes and incubated overnight to 70% confluence.

    Techniques: Neutralization, MANN-WHITNEY

    The study of lymphoproliferative activity of splenocytes in immunized mice. The figure shows the levels (%) of proliferating CD4+ and CD8+T cells re-stimulated by the MERS-CoV S protein on day 8 post-vaccination. Scatter plots show the median lymphoproliferative activity of re-stimulated cells (%) with 95% CIs for each group from one representative experiment (n = 6 mice/group). Asterisks indicate significant differences in the percentage of proliferating cells between vaccinated and intact animals. * p

    Journal: Acta Naturae

    Article Title: Immunogenicity of Different Forms of Middle East Respiratory Syndrome S Glycoprotein

    doi:

    Figure Lengend Snippet: The study of lymphoproliferative activity of splenocytes in immunized mice. The figure shows the levels (%) of proliferating CD4+ and CD8+T cells re-stimulated by the MERS-CoV S protein on day 8 post-vaccination. Scatter plots show the median lymphoproliferative activity of re-stimulated cells (%) with 95% CIs for each group from one representative experiment (n = 6 mice/group). Asterisks indicate significant differences in the percentage of proliferating cells between vaccinated and intact animals. * p

    Article Snippet: Evaluating the expression of different forms of the MERS-CoV S glycoprotein by western blottingHEK293 cells were seeded in 35 mm Petri dishes and incubated overnight to 70% confluence.

    Techniques: Activity Assay, Mouse Assay