anti bcl2 antibody  (Sino Biological)


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    Name:
    BCL2 Bcl 2 Antibody APC Rabbit MAb
    Description:
    This antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to the N terminus of the Human BCL2 Bcl 2 and conjugated with APC under optimum conditions the unreacted APC was removed
    Catalog Number:
    100126-r204-a
    Price:
    None
    Category:
    Primary Antibody
    Reactivity:
    Human
    Applications:
    FCM
    Immunogen:
    A synthetic peptide corresponding to the N-terminus of the Human BCL2/Bcl-2
    Antibody Type:
    MAb
    Host:
    Rabbit
    Isotype:
    Rabbit IgG
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    Structured Review

    Sino Biological anti bcl2 antibody
    Melatonin accelerated the proliferation of Leydig cells and restrained its apoptosis. a Morphological comparison of MLTC-1 was performed when cultured for 48 h in four different groups (C control, M melatonin treatment, G high glucose treatment, GM high glucose in combination with melatonin treatment). Scale bar, 100 μm. b – d BrdU incorporation assay detected the proliferative ability in four different groups which were all treated for 48 h. Immunofluorescence images show an increase in incidence of BrdU-positive cells in GM group compared to G group. Scale bar, 200 μm. Histogram was the statistical BrdU-positive rate for b . c – e TUNEL assay detected the apoptosis rate in four different groups, which were all treated for 48 h. Immunofluorescence images show a decrease in incidence of TUNEL-positive cells in GM group compared to G group. Scale bar, 200 μm. Histogram is the statistical TUNEL positive rate for c . f – h MLTC-1 apoptosis rate ( f ) and cell cycle distribution ( h ) was determined by FCM in four different treatments. g RT-qPCR examined the expression of apoptosis-related genes ( P53, <t>Bcl2,</t> Caspase3, Caspase12 and P21 ) and ERS-related genes ( Grp78 and Chop ) in the four different groups of MLTC-1. i Western blot analysis of apoptosis- and ERS-related genes in MLTC-1 under four different treatments. j Histograms show quantitative results of Image J (V1.48d) gradation analysis for the western blot experiments in i . (The results are shown as the mean ± S.E.M of four separated wells of cells ( n = 4) in at least three different experiments and the statistical significance is expressed as follows: * p
    This antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to the N terminus of the Human BCL2 Bcl 2 and conjugated with APC under optimum conditions the unreacted APC was removed
    https://www.bioz.com/result/anti bcl2 antibody/product/Sino Biological
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti bcl2 antibody - by Bioz Stars, 2021-03
    91/100 stars

    Images

    1) Product Images from "Melatonin attenuates detrimental effects of diabetes on the niche of mouse spermatogonial stem cells by maintaining Leydig cells"

    Article Title: Melatonin attenuates detrimental effects of diabetes on the niche of mouse spermatogonial stem cells by maintaining Leydig cells

    Journal: Cell Death & Disease

    doi: 10.1038/s41419-018-0956-4

    Melatonin accelerated the proliferation of Leydig cells and restrained its apoptosis. a Morphological comparison of MLTC-1 was performed when cultured for 48 h in four different groups (C control, M melatonin treatment, G high glucose treatment, GM high glucose in combination with melatonin treatment). Scale bar, 100 μm. b – d BrdU incorporation assay detected the proliferative ability in four different groups which were all treated for 48 h. Immunofluorescence images show an increase in incidence of BrdU-positive cells in GM group compared to G group. Scale bar, 200 μm. Histogram was the statistical BrdU-positive rate for b . c – e TUNEL assay detected the apoptosis rate in four different groups, which were all treated for 48 h. Immunofluorescence images show a decrease in incidence of TUNEL-positive cells in GM group compared to G group. Scale bar, 200 μm. Histogram is the statistical TUNEL positive rate for c . f – h MLTC-1 apoptosis rate ( f ) and cell cycle distribution ( h ) was determined by FCM in four different treatments. g RT-qPCR examined the expression of apoptosis-related genes ( P53, Bcl2, Caspase3, Caspase12 and P21 ) and ERS-related genes ( Grp78 and Chop ) in the four different groups of MLTC-1. i Western blot analysis of apoptosis- and ERS-related genes in MLTC-1 under four different treatments. j Histograms show quantitative results of Image J (V1.48d) gradation analysis for the western blot experiments in i . (The results are shown as the mean ± S.E.M of four separated wells of cells ( n = 4) in at least three different experiments and the statistical significance is expressed as follows: * p
    Figure Legend Snippet: Melatonin accelerated the proliferation of Leydig cells and restrained its apoptosis. a Morphological comparison of MLTC-1 was performed when cultured for 48 h in four different groups (C control, M melatonin treatment, G high glucose treatment, GM high glucose in combination with melatonin treatment). Scale bar, 100 μm. b – d BrdU incorporation assay detected the proliferative ability in four different groups which were all treated for 48 h. Immunofluorescence images show an increase in incidence of BrdU-positive cells in GM group compared to G group. Scale bar, 200 μm. Histogram was the statistical BrdU-positive rate for b . c – e TUNEL assay detected the apoptosis rate in four different groups, which were all treated for 48 h. Immunofluorescence images show a decrease in incidence of TUNEL-positive cells in GM group compared to G group. Scale bar, 200 μm. Histogram is the statistical TUNEL positive rate for c . f – h MLTC-1 apoptosis rate ( f ) and cell cycle distribution ( h ) was determined by FCM in four different treatments. g RT-qPCR examined the expression of apoptosis-related genes ( P53, Bcl2, Caspase3, Caspase12 and P21 ) and ERS-related genes ( Grp78 and Chop ) in the four different groups of MLTC-1. i Western blot analysis of apoptosis- and ERS-related genes in MLTC-1 under four different treatments. j Histograms show quantitative results of Image J (V1.48d) gradation analysis for the western blot experiments in i . (The results are shown as the mean ± S.E.M of four separated wells of cells ( n = 4) in at least three different experiments and the statistical significance is expressed as follows: * p

    Techniques Used: Cell Culture, BrdU Incorporation Assay, Immunofluorescence, TUNEL Assay, Quantitative RT-PCR, Expressing, Western Blot

    Effect of melatonin on the proliferation, cell survival, and spermatogenic function of testicular tissue. a PCNA expression in testes of four different treatment groups in short term (W2, 2 weeks) and long term (W8, 8 weeks) experiments. Scale bar, 200 μm. b Cell apoptosis rate was determined by flow cytometry (FCM). c Epididymal semen smears of four different groups in W8 experiments. Scale bar, 100 μm. Black arrows in D8 show the abnormal characteristics. d RT-qPCR analysis of apoptosis-related genes ( P53, Bcl2, Caspase 3 and Caspase 12 ) and endoplasmic reticulum stress-related genes (Grp78 and Chop) in testicular cells suspensions of four different groups in W2 and W8 experiments. e Western blot analysis of apoptosis-related genes in testicular cell suspensions of four different groups in W2 and W8 experiments. The histograms show quantitative results of Image J (V1.48d) gradation analysis for the western blot experiments. (The results are expressed as the mean ± S.E.M of at least three mice ( n = 3) and the statistical significance is expressed as follows: * p
    Figure Legend Snippet: Effect of melatonin on the proliferation, cell survival, and spermatogenic function of testicular tissue. a PCNA expression in testes of four different treatment groups in short term (W2, 2 weeks) and long term (W8, 8 weeks) experiments. Scale bar, 200 μm. b Cell apoptosis rate was determined by flow cytometry (FCM). c Epididymal semen smears of four different groups in W8 experiments. Scale bar, 100 μm. Black arrows in D8 show the abnormal characteristics. d RT-qPCR analysis of apoptosis-related genes ( P53, Bcl2, Caspase 3 and Caspase 12 ) and endoplasmic reticulum stress-related genes (Grp78 and Chop) in testicular cells suspensions of four different groups in W2 and W8 experiments. e Western blot analysis of apoptosis-related genes in testicular cell suspensions of four different groups in W2 and W8 experiments. The histograms show quantitative results of Image J (V1.48d) gradation analysis for the western blot experiments. (The results are expressed as the mean ± S.E.M of at least three mice ( n = 3) and the statistical significance is expressed as follows: * p

    Techniques Used: Expressing, Flow Cytometry, Quantitative RT-PCR, Western Blot, Mouse Assay

    Related Articles

    Western Blot:

    Article Title: Melatonin attenuates detrimental effects of diabetes on the niche of mouse spermatogonial stem cells by maintaining Leydig cells
    Article Snippet: After denaturation by heating for 10 min at 100 °C in 5% SDS-PAGE loading buffer, the protein sample was resolved by SDS-PAGE and transferred to a PVDF membrane (61). .. The primary antibodies used for Western blot analysis included anti-P53 antibody (1:1000, mouse IgG, Sino Biological Inc., Beijing, PRC), anti-Bcl2 antibody (1:1000, Sino Biological Inc.), anti-Bax antibody (1:1,000, rabbit IgG, Cell Signaling Technology, Danvers, MA, USA), anti-Caspase 3 antibody (1:1000, mouse IgG, Cell Signaling Technology), anti-Chop antibody (1:1,000, mouse IgG, Sangon), anti-Grp78 antibody (1:1000, rabbit IgG Sangon), anti-CSF1 antibody (1:1000, rabbit IgG, Sangon), anti-CSF1r antibody (1:1000, mouse IgG, Sangon), and anti-β-actin antibody (1:5,000, mouse IgG2b, Cell Signaling Technology). .. The protein bands were detected using a Bio-Rad imaging system (Bio-Rad, Hercules, CA, USA) and quantified using Image J (V1.48d).

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  • 91
    Sino Biological anti bcl2 antibody
    Melatonin accelerated the proliferation of Leydig cells and restrained its apoptosis. a Morphological comparison of MLTC-1 was performed when cultured for 48 h in four different groups (C control, M melatonin treatment, G high glucose treatment, GM high glucose in combination with melatonin treatment). Scale bar, 100 μm. b – d BrdU incorporation assay detected the proliferative ability in four different groups which were all treated for 48 h. Immunofluorescence images show an increase in incidence of BrdU-positive cells in GM group compared to G group. Scale bar, 200 μm. Histogram was the statistical BrdU-positive rate for b . c – e TUNEL assay detected the apoptosis rate in four different groups, which were all treated for 48 h. Immunofluorescence images show a decrease in incidence of TUNEL-positive cells in GM group compared to G group. Scale bar, 200 μm. Histogram is the statistical TUNEL positive rate for c . f – h MLTC-1 apoptosis rate ( f ) and cell cycle distribution ( h ) was determined by FCM in four different treatments. g RT-qPCR examined the expression of apoptosis-related genes ( P53, <t>Bcl2,</t> Caspase3, Caspase12 and P21 ) and ERS-related genes ( Grp78 and Chop ) in the four different groups of MLTC-1. i Western blot analysis of apoptosis- and ERS-related genes in MLTC-1 under four different treatments. j Histograms show quantitative results of Image J (V1.48d) gradation analysis for the western blot experiments in i . (The results are shown as the mean ± S.E.M of four separated wells of cells ( n = 4) in at least three different experiments and the statistical significance is expressed as follows: * p
    Anti Bcl2 Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti bcl2 antibody/product/Sino Biological
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti bcl2 antibody - by Bioz Stars, 2021-03
    91/100 stars
      Buy from Supplier

    Image Search Results


    Melatonin accelerated the proliferation of Leydig cells and restrained its apoptosis. a Morphological comparison of MLTC-1 was performed when cultured for 48 h in four different groups (C control, M melatonin treatment, G high glucose treatment, GM high glucose in combination with melatonin treatment). Scale bar, 100 μm. b – d BrdU incorporation assay detected the proliferative ability in four different groups which were all treated for 48 h. Immunofluorescence images show an increase in incidence of BrdU-positive cells in GM group compared to G group. Scale bar, 200 μm. Histogram was the statistical BrdU-positive rate for b . c – e TUNEL assay detected the apoptosis rate in four different groups, which were all treated for 48 h. Immunofluorescence images show a decrease in incidence of TUNEL-positive cells in GM group compared to G group. Scale bar, 200 μm. Histogram is the statistical TUNEL positive rate for c . f – h MLTC-1 apoptosis rate ( f ) and cell cycle distribution ( h ) was determined by FCM in four different treatments. g RT-qPCR examined the expression of apoptosis-related genes ( P53, Bcl2, Caspase3, Caspase12 and P21 ) and ERS-related genes ( Grp78 and Chop ) in the four different groups of MLTC-1. i Western blot analysis of apoptosis- and ERS-related genes in MLTC-1 under four different treatments. j Histograms show quantitative results of Image J (V1.48d) gradation analysis for the western blot experiments in i . (The results are shown as the mean ± S.E.M of four separated wells of cells ( n = 4) in at least three different experiments and the statistical significance is expressed as follows: * p

    Journal: Cell Death & Disease

    Article Title: Melatonin attenuates detrimental effects of diabetes on the niche of mouse spermatogonial stem cells by maintaining Leydig cells

    doi: 10.1038/s41419-018-0956-4

    Figure Lengend Snippet: Melatonin accelerated the proliferation of Leydig cells and restrained its apoptosis. a Morphological comparison of MLTC-1 was performed when cultured for 48 h in four different groups (C control, M melatonin treatment, G high glucose treatment, GM high glucose in combination with melatonin treatment). Scale bar, 100 μm. b – d BrdU incorporation assay detected the proliferative ability in four different groups which were all treated for 48 h. Immunofluorescence images show an increase in incidence of BrdU-positive cells in GM group compared to G group. Scale bar, 200 μm. Histogram was the statistical BrdU-positive rate for b . c – e TUNEL assay detected the apoptosis rate in four different groups, which were all treated for 48 h. Immunofluorescence images show a decrease in incidence of TUNEL-positive cells in GM group compared to G group. Scale bar, 200 μm. Histogram is the statistical TUNEL positive rate for c . f – h MLTC-1 apoptosis rate ( f ) and cell cycle distribution ( h ) was determined by FCM in four different treatments. g RT-qPCR examined the expression of apoptosis-related genes ( P53, Bcl2, Caspase3, Caspase12 and P21 ) and ERS-related genes ( Grp78 and Chop ) in the four different groups of MLTC-1. i Western blot analysis of apoptosis- and ERS-related genes in MLTC-1 under four different treatments. j Histograms show quantitative results of Image J (V1.48d) gradation analysis for the western blot experiments in i . (The results are shown as the mean ± S.E.M of four separated wells of cells ( n = 4) in at least three different experiments and the statistical significance is expressed as follows: * p

    Article Snippet: The primary antibodies used for Western blot analysis included anti-P53 antibody (1:1000, mouse IgG, Sino Biological Inc., Beijing, PRC), anti-Bcl2 antibody (1:1000, Sino Biological Inc.), anti-Bax antibody (1:1,000, rabbit IgG, Cell Signaling Technology, Danvers, MA, USA), anti-Caspase 3 antibody (1:1000, mouse IgG, Cell Signaling Technology), anti-Chop antibody (1:1,000, mouse IgG, Sangon), anti-Grp78 antibody (1:1000, rabbit IgG Sangon), anti-CSF1 antibody (1:1000, rabbit IgG, Sangon), anti-CSF1r antibody (1:1000, mouse IgG, Sangon), and anti-β-actin antibody (1:5,000, mouse IgG2b, Cell Signaling Technology).

    Techniques: Cell Culture, BrdU Incorporation Assay, Immunofluorescence, TUNEL Assay, Quantitative RT-PCR, Expressing, Western Blot

    Effect of melatonin on the proliferation, cell survival, and spermatogenic function of testicular tissue. a PCNA expression in testes of four different treatment groups in short term (W2, 2 weeks) and long term (W8, 8 weeks) experiments. Scale bar, 200 μm. b Cell apoptosis rate was determined by flow cytometry (FCM). c Epididymal semen smears of four different groups in W8 experiments. Scale bar, 100 μm. Black arrows in D8 show the abnormal characteristics. d RT-qPCR analysis of apoptosis-related genes ( P53, Bcl2, Caspase 3 and Caspase 12 ) and endoplasmic reticulum stress-related genes (Grp78 and Chop) in testicular cells suspensions of four different groups in W2 and W8 experiments. e Western blot analysis of apoptosis-related genes in testicular cell suspensions of four different groups in W2 and W8 experiments. The histograms show quantitative results of Image J (V1.48d) gradation analysis for the western blot experiments. (The results are expressed as the mean ± S.E.M of at least three mice ( n = 3) and the statistical significance is expressed as follows: * p

    Journal: Cell Death & Disease

    Article Title: Melatonin attenuates detrimental effects of diabetes on the niche of mouse spermatogonial stem cells by maintaining Leydig cells

    doi: 10.1038/s41419-018-0956-4

    Figure Lengend Snippet: Effect of melatonin on the proliferation, cell survival, and spermatogenic function of testicular tissue. a PCNA expression in testes of four different treatment groups in short term (W2, 2 weeks) and long term (W8, 8 weeks) experiments. Scale bar, 200 μm. b Cell apoptosis rate was determined by flow cytometry (FCM). c Epididymal semen smears of four different groups in W8 experiments. Scale bar, 100 μm. Black arrows in D8 show the abnormal characteristics. d RT-qPCR analysis of apoptosis-related genes ( P53, Bcl2, Caspase 3 and Caspase 12 ) and endoplasmic reticulum stress-related genes (Grp78 and Chop) in testicular cells suspensions of four different groups in W2 and W8 experiments. e Western blot analysis of apoptosis-related genes in testicular cell suspensions of four different groups in W2 and W8 experiments. The histograms show quantitative results of Image J (V1.48d) gradation analysis for the western blot experiments. (The results are expressed as the mean ± S.E.M of at least three mice ( n = 3) and the statistical significance is expressed as follows: * p

    Article Snippet: The primary antibodies used for Western blot analysis included anti-P53 antibody (1:1000, mouse IgG, Sino Biological Inc., Beijing, PRC), anti-Bcl2 antibody (1:1000, Sino Biological Inc.), anti-Bax antibody (1:1,000, rabbit IgG, Cell Signaling Technology, Danvers, MA, USA), anti-Caspase 3 antibody (1:1000, mouse IgG, Cell Signaling Technology), anti-Chop antibody (1:1,000, mouse IgG, Sangon), anti-Grp78 antibody (1:1000, rabbit IgG Sangon), anti-CSF1 antibody (1:1000, rabbit IgG, Sangon), anti-CSF1r antibody (1:1000, mouse IgG, Sangon), and anti-β-actin antibody (1:5,000, mouse IgG2b, Cell Signaling Technology).

    Techniques: Expressing, Flow Cytometry, Quantitative RT-PCR, Western Blot, Mouse Assay