100 bp dna ladder  (Thermo Fisher)


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    Structured Review

    Thermo Fisher 100 bp dna ladder
    Electrophoresis gel (agarose 2%) in which it is possible to observe nitid positive amplicons: nested PCR products (145 <t>bp):</t> ORF2 gene. Wells loading: S = <t>DNA</t> <t>ladder</t> 50 bp (Genetics® FastGene 50 bp DNA Marker) loaded into the first and last wells. Each line corresponds to 50 bp. 1 = K+ / Positive control (ATCC® VR-3258SD RNA fragment). 6 = K- / Negative control. 2, 3, 5, 7, 8, 12, 13, 14 = Positive samples (samples ID and respective information are reported in Table 3 ). 4, 9, 10, 11, 15, 16, 17, 18 = Negative samples.
    100 Bp Dna Ladder, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/100 bp dna ladder/product/Thermo Fisher
    Average 98 stars, based on 40 article reviews
    Price from $9.99 to $1999.99
    100 bp dna ladder - by Bioz Stars, 2022-11
    98/100 stars

    Images

    1) Product Images from "Hepatitis E virus detection in hunted wild boar (Sus scrofa) livers in Central Italy"

    Article Title: Hepatitis E virus detection in hunted wild boar (Sus scrofa) livers in Central Italy

    Journal: Italian Journal of Food Safety

    doi: 10.4081/ijfs.2022.9979

    Electrophoresis gel (agarose 2%) in which it is possible to observe nitid positive amplicons: nested PCR products (145 bp): ORF2 gene. Wells loading: S = DNA ladder 50 bp (Genetics® FastGene 50 bp DNA Marker) loaded into the first and last wells. Each line corresponds to 50 bp. 1 = K+ / Positive control (ATCC® VR-3258SD RNA fragment). 6 = K- / Negative control. 2, 3, 5, 7, 8, 12, 13, 14 = Positive samples (samples ID and respective information are reported in Table 3 ). 4, 9, 10, 11, 15, 16, 17, 18 = Negative samples.
    Figure Legend Snippet: Electrophoresis gel (agarose 2%) in which it is possible to observe nitid positive amplicons: nested PCR products (145 bp): ORF2 gene. Wells loading: S = DNA ladder 50 bp (Genetics® FastGene 50 bp DNA Marker) loaded into the first and last wells. Each line corresponds to 50 bp. 1 = K+ / Positive control (ATCC® VR-3258SD RNA fragment). 6 = K- / Negative control. 2, 3, 5, 7, 8, 12, 13, 14 = Positive samples (samples ID and respective information are reported in Table 3 ). 4, 9, 10, 11, 15, 16, 17, 18 = Negative samples.

    Techniques Used: Electrophoresis, Nested PCR, Marker, Positive Control, Negative Control

    2) Product Images from "DNA Adduct Detection after Post-Labeling Technique with PCR Amplification (DNA-ADAPT–qPCR) Identifies the Pre-Ribosomal RNA Gene as a Direct Target of Platinum–Acridine Anticancer Agents"

    Article Title: DNA Adduct Detection after Post-Labeling Technique with PCR Amplification (DNA-ADAPT–qPCR) Identifies the Pre-Ribosomal RNA Gene as a Direct Target of Platinum–Acridine Anticancer Agents

    Journal: Chemistry (Weinheim an der Bergstrasse, Germany)

    doi: 10.1002/chem.202102263

    Validation and optimization of DNA purification by affinity capture in model duplexes. (A) Assay design for plasmid DNA: (a) Preparation of linearized forms of two plasmids; (b) treatment of pBR322 with APA and post-labeling with DBCO-PEG 4 -BIOTIN (2 steps); (c) pooling of unmodified pUC19 and APA-modified pBR322 plasmid samples; (d) affinity capture on beads (red spheres) and (e) removal of nonspecifically bound plasmid; (f) dissociation of affinity-captured pBR322 and detection/quantification by gel electrophoresis. (B) Agarose gel for affinity purification of plasmid DNA. The recovered pBR322 (8%) is highlighted with a red box. (C) Agarose gel for affinity purification of a DNA ladder. The DNA was modified with APA at a drug-to-base pair ratio of 0.02. Lane assignments: 1-unpurified DNA, 2-unplatinated/unbiotinylated control, 3-unplatinated control, 4-ADAPT-purified DNA. The estimated cumulative recovery of DNA (lane 4 vs. lane 1) is 11% (Image J software). (An accurate quantification was not possible because of variabilities in the ethidium staining efficiency for individual fragments.)
    Figure Legend Snippet: Validation and optimization of DNA purification by affinity capture in model duplexes. (A) Assay design for plasmid DNA: (a) Preparation of linearized forms of two plasmids; (b) treatment of pBR322 with APA and post-labeling with DBCO-PEG 4 -BIOTIN (2 steps); (c) pooling of unmodified pUC19 and APA-modified pBR322 plasmid samples; (d) affinity capture on beads (red spheres) and (e) removal of nonspecifically bound plasmid; (f) dissociation of affinity-captured pBR322 and detection/quantification by gel electrophoresis. (B) Agarose gel for affinity purification of plasmid DNA. The recovered pBR322 (8%) is highlighted with a red box. (C) Agarose gel for affinity purification of a DNA ladder. The DNA was modified with APA at a drug-to-base pair ratio of 0.02. Lane assignments: 1-unpurified DNA, 2-unplatinated/unbiotinylated control, 3-unplatinated control, 4-ADAPT-purified DNA. The estimated cumulative recovery of DNA (lane 4 vs. lane 1) is 11% (Image J software). (An accurate quantification was not possible because of variabilities in the ethidium staining efficiency for individual fragments.)

    Techniques Used: DNA Purification, Plasmid Preparation, Labeling, Modification, Nucleic Acid Electrophoresis, Agarose Gel Electrophoresis, Affinity Purification, Purification, Software, Staining

    3) Product Images from "Detection of Human Papillomavirus and p16INK4a Expression in Thai Patients with Oral Squamous Cell Carcinoma"

    Article Title: Detection of Human Papillomavirus and p16INK4a Expression in Thai Patients with Oral Squamous Cell Carcinoma

    Journal: Head and Neck Pathology

    doi: 10.1007/s12105-021-01381-x

    Representative images of PCR products. PCR analysis of GAPDH gene ( A ). Lane M: 100-bp DNA ladder marker, lane 1: negative control, lane 2: positive control, lanes 3–10: OSCC samples with GAPDH amplification. First step of nested PCR using MY09-MY011 primers ( B ). Lane M: 100-bp DNA ladder marker, lane 1: negative control, lane 2: positive control, lanes 3–10: representative OSCC samples. Second step of nested PCR using HPV1003-HPV1004 primers ( C ). Lane M: 100-bp DNA ladder marker, lane 1: negative control, lane 2: positive control, lane 3: PCR product of the first step negative control, lane 4: PCR product of the first step positive control, lanes 5–8: OSCC samples with positive results, lanes 9–12: OSCC samples with negative results
    Figure Legend Snippet: Representative images of PCR products. PCR analysis of GAPDH gene ( A ). Lane M: 100-bp DNA ladder marker, lane 1: negative control, lane 2: positive control, lanes 3–10: OSCC samples with GAPDH amplification. First step of nested PCR using MY09-MY011 primers ( B ). Lane M: 100-bp DNA ladder marker, lane 1: negative control, lane 2: positive control, lanes 3–10: representative OSCC samples. Second step of nested PCR using HPV1003-HPV1004 primers ( C ). Lane M: 100-bp DNA ladder marker, lane 1: negative control, lane 2: positive control, lane 3: PCR product of the first step negative control, lane 4: PCR product of the first step positive control, lanes 5–8: OSCC samples with positive results, lanes 9–12: OSCC samples with negative results

    Techniques Used: Polymerase Chain Reaction, Marker, Negative Control, Positive Control, Amplification, Nested PCR

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