10×phi29 buffer  (Thermo Fisher)


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  • 98
    Name:
    Reaction Buffer for phi29 DNA Polymerase 10X
    Description:
    Thermo Scientific 10X Buffer for phi29 DNA Polymerase is the optimal buffer recommended for use with highly processive phi 29 DNA polymerase
    Catalog Number:
    b62
    Price:
    None
    Applications:
    Cloning|DNA & RNA Purification & Analysis|Gene Synthesis, Seamless Cloning, & Assembly|Seamless Cloning & Genetic Assembly
    Category:
    Lab Reagents and Chemicals
    Buy from Supplier


    Structured Review

    Thermo Fisher 10×phi29 buffer
    Thermo Scientific 10X Buffer for phi29 DNA Polymerase is the optimal buffer recommended for use with highly processive phi 29 DNA polymerase
    https://www.bioz.com/result/10×phi29 buffer/product/Thermo Fisher
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    10×phi29 buffer - by Bioz Stars, 2020-11
    98/100 stars

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    random octamer primer
    bsa

    Images

    Related Articles

    Amplification:

    Article Title: Ictal onset sites and GABAergic neuron loss in epileptic pilocarpine-treated rats
    Article Snippet: .. Amplification solution contained 1000 units/ml NxGen phi29 DNA polymerase in phi29 polymerase buffer (Lucigen) and 1 mM dNTP mix (0.25 mM/nucleotide, Invitrogen), 0.4 units/μl RNaseOUT, 5% glycerol, and 0.4 mg/ml BSA; sections incubated overnight at 37°C. .. The next day sections were rinsed in a label probe hybridization buffer of 2x saline-sodium citrate (Invitrogen) and 20% deionized formamide (Invitrogen) in DEPC-treated water.

    Article Title: Ictal onset sites and GABAergic neuron loss in epileptic pilocarpine-treated rats
    Article Snippet: .. Sections were rinsed in hybridization buffer followed by phi29 polymerase buffer before the rolling circle amplification reaction. .. Amplification solution contained 1000 units/ml NxGen phi29 DNA polymerase in phi29 polymerase buffer (Lucigen) and 1 mM dNTP mix (0.25 mM/nucleotide, Invitrogen), 0.4 units/μl RNaseOUT, 5% glycerol, and 0.4 mg/ml BSA; sections incubated overnight at 37°C.

    Cell Culture:

    Article Title: Immune-privileged embryonic Swiss mouse STO and STO cell-derived progenitor cells: major histocompatibility complex and cell differentiation antigen expression patterns resemble those of human embryonic stem cell lines
    Article Snippet: .. STO and SCD lines 3(8)21 and 3(8)21-EGFP were cultured by standard procedures; , passage numbers were recorded and cell counts performed as described elsewhere., Swiss mouse q-haplotype NIH3T3 cells, d-haplotype BALB/c 3T3 cells, human embryonic kidney 293 cells (American Type Culture Collection #CRL-1573), mouse H-2Kb/d -, H-2Db/d - and H-2Lb/d -positive B6-2 cells and primary BALB/c kidney cells prepared from trypsinized tissue were cultured as described previously., Fetal bovine serum (FBS; Invitrogen, Carlsbad, CA) was heat-inactivated at 56° for 30 min. .. Unless noted, cells were cultured in 3- or 10-cm-diameter plastic tissue culture dishes (BD Falcon™, Bedford, MA).

    TaqMan microRNA Assay:

    Article Title: Human hepatocellular carcinoma cell-specific miRNAs reveal the differential expression of miR-24 and miR-27a in cirrhotic/non-cirrhotic HCC
    Article Snippet: .. The reverse transcriptase reaction was performed by incubating the samples at 16°C for 30 min, 42°C for 30 min, and 85°C for 5 min. Each PCR reaction (20 μ l) contained 1.3 μ l of reverse transcriptase product, 10 μ l of Taq-Man 2X Universal PCR Master Mix, and 1 μ l of the appropriate TaqMan MicroRNA Assay solution containing primers and probes for each miR of interest. .. The PCR mixtures were incubated at 95°C for 10 min followed by 40 cycles of 95°C for 15 sec and 60°C for 60 sec. PCRs were performed in triplicate using a 7500 Real-Time PCR system.

    Concentration Assay:

    Article Title: Recording the age of RNA with deamination
    Article Snippet: .. Cell concentration was counted and the appropriate volume of cells was added to a 10x reaction for a targeted cell recovery of 2000 cells. .. The reaction was run through a 10x chip according to the 10x Genomics Chromium Single Cell protocol.

    Incubation:

    Article Title: Ictal onset sites and GABAergic neuron loss in epileptic pilocarpine-treated rats
    Article Snippet: .. Amplification solution contained 1000 units/ml NxGen phi29 DNA polymerase in phi29 polymerase buffer (Lucigen) and 1 mM dNTP mix (0.25 mM/nucleotide, Invitrogen), 0.4 units/μl RNaseOUT, 5% glycerol, and 0.4 mg/ml BSA; sections incubated overnight at 37°C. .. The next day sections were rinsed in a label probe hybridization buffer of 2x saline-sodium citrate (Invitrogen) and 20% deionized formamide (Invitrogen) in DEPC-treated water.

    other:

    Article Title: microRNA-944 overexpression is a biomarker for poor prognosis of advanced cervical cancer
    Article Snippet: The reverse transcriptase reaction was carried out at 25 °C for 10 min, at 37 °C for 50 min, and at 70 °C for 15 min.

    Article Title: MicroRNA-103-1 Selectively Downregulates Brain NCX1 and Its Inhibition by Anti-miRNA Ameliorates Stroke Damage and Neurological Deficits
    Article Snippet: The semiquantitative polymerase reaction was performed as previously described.

    Polymerase Chain Reaction:

    Article Title: Human hepatocellular carcinoma cell-specific miRNAs reveal the differential expression of miR-24 and miR-27a in cirrhotic/non-cirrhotic HCC
    Article Snippet: .. The reverse transcriptase reaction was performed by incubating the samples at 16°C for 30 min, 42°C for 30 min, and 85°C for 5 min. Each PCR reaction (20 μ l) contained 1.3 μ l of reverse transcriptase product, 10 μ l of Taq-Man 2X Universal PCR Master Mix, and 1 μ l of the appropriate TaqMan MicroRNA Assay solution containing primers and probes for each miR of interest. .. The PCR mixtures were incubated at 95°C for 10 min followed by 40 cycles of 95°C for 15 sec and 60°C for 60 sec. PCRs were performed in triplicate using a 7500 Real-Time PCR system.

    Hybridization:

    Article Title: Ictal onset sites and GABAergic neuron loss in epileptic pilocarpine-treated rats
    Article Snippet: .. Sections were rinsed in hybridization buffer followed by phi29 polymerase buffer before the rolling circle amplification reaction. .. Amplification solution contained 1000 units/ml NxGen phi29 DNA polymerase in phi29 polymerase buffer (Lucigen) and 1 mM dNTP mix (0.25 mM/nucleotide, Invitrogen), 0.4 units/μl RNaseOUT, 5% glycerol, and 0.4 mg/ml BSA; sections incubated overnight at 37°C.