10 beta electrocompetent cells  (New England Biolabs)


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    Name:
    NEB 10 beta Electrocompetent E coli
    Description:
    NEB 10 beta Electrocompetent E coli 6x0 1 ml
    Catalog Number:
    c3020k
    Price:
    216
    Size:
    0 6 ml
    Category:
    Competent Bacteria
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    New England Biolabs 10 beta electrocompetent cells
    NEB 10 beta Electrocompetent E coli
    NEB 10 beta Electrocompetent E coli 6x0 1 ml
    https://www.bioz.com/result/10 beta electrocompetent cells/product/New England Biolabs
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    10 beta electrocompetent cells - by Bioz Stars, 2020-08
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Harmonious genetic combinations rewire regulatory networks and flip gene essentiality
    Article Snippet: .. Clones were transformed into electrocompetent 10-Beta cells (New England Biolabs # C3020) using a 96-well plate electroporator using fresh electroporation plates (BTX 45–0450-M). .. Cells were recovered in deep-well plates (Thermo Scientific # 260252) and recovered in 0.6 ml SOC media for 1 h prior to inoculation into 0.6 ml LB + 100 mg/ml ampicillin.

    Electroporation:

    Article Title: Harmonious genetic combinations rewire regulatory networks and flip gene essentiality
    Article Snippet: .. Clones were transformed into electrocompetent 10-Beta cells (New England Biolabs # C3020) using a 96-well plate electroporator using fresh electroporation plates (BTX 45–0450-M). .. Cells were recovered in deep-well plates (Thermo Scientific # 260252) and recovered in 0.6 ml SOC media for 1 h prior to inoculation into 0.6 ml LB + 100 mg/ml ampicillin.

    Article Title: Prioritizing disease and trait causal variants at the TNFAIP3 locus using functional and genomic features
    Article Snippet: .. Half of the ligated vector was then transformed into 100 μL of 10-beta e.coli (NEB, C3020K) by electroporation (2 kV, 200 Ω, 25 μF). .. Electroporated bacteria were immediately split into eight 1 mL aliquots of SOC (NEB, B9020S) and recovered for 1 h at 37 °C then independently expanded in 20 mL of LB supplemented with 100 μg/mL of carbenicillin (EMD, 69101-3) on a floor shaker at 37 °C for 6.5 h. After outgrowth aliquots were pooled prior to plasmid purification (QIAGEN, 12963).

    Article Title: Circular synthesized CRISPR/Cas gRNAs for functional interrogations in the coding and noncoding genome
    Article Snippet: .. Electroporation and I-SceI clean-up digest 2 mm cuvette (BTX, 45–0125), electrocompetent E. coli (10-beta, NEB, C3020K), SOC outgrowth medium (Thermo Fisher, 15544034), LB-media (Roth, X964.3) supplemented with 100 µg/ml ampicillin, Qiagen Plasmid Maxi Kit (Qiagen, 12163), I-SceI (NEB, R0694), NEB CutSmart buffer (NEB, B7204), 0.5% TAE/agarose gel, Thermo Fisher Scientific GeneJET Gel Extraction Kit. .. dU-ssDNA template amplification Bacteria (Escherichia coli strain K12 CJ236, NEB, E4141) were transformed with 500 ng of template plasmid according to the following protocol: DNA was mixed with 2 µl of 5x KCM buffer (0.5M KCl, 0.15M CaCl2 , 0.25M MgCl2 ) set to 10 µl with water and chilled on ice for 10 min. An equal volume of CJ236 bacteria was added to the DNA/KCM mixture, gently mixed, and incubated on ice for 15 min.

    Purification:

    Article Title: Predictable and precise template-free CRISPR editing of pathogenic variants
    Article Snippet: .. Assembled plasmids were purified by isopropanol precipitation with GlycoBlue Coprecipitant (Thermo Fisher) and reconstituted in milliQ water and transformed into NEB10beta (New England Biolabs) electrocompetent cells. .. Following recovery, a small dilution series was plated to assess transformation efficiency and the remainder was grown in liquid culture in DRM medium overnight at 37°C.

    Article Title: Precise tuning of gene expression levels in mammalian cells
    Article Snippet: .. We purified the ligations using the QIAquick PCR Purification Kit (Qiagen) and transformed approximately 3.6 μg of the purified product into 10-beta Electrocompetent E.coli (NEB) following the manufacturer’s instructions. ..

    Incubation:

    Article Title: Modular assembly of transposon integratable multigene vectors using RecWay assembly
    Article Snippet: .. Cre-mediated retrofitting was performed using 100 ng of self-ligated shuttle vector and 100 ng of expression vector in a 20 µL of reaction using 1 Unit of Cre recombinase (New England BioLabs) incubated at 37°C for 1 h. Ten microliters of the Cre reaction was then transformed into top 10 bacteria (Invitrogen) or electroporated into 10-beta Electrocompetent Escherichia coli (New England BioLabs). .. FLP recombination For removal of flippase recognition target (FRT)-flanked kanamycin, chloramphenicol, and spectinomycin cassettes, plasmids were transformed into Arabinose-induced flippase (FLP) recombinase-expressing bacteria EL250, as previously described ( ).

    Expressing:

    Article Title: Modular assembly of transposon integratable multigene vectors using RecWay assembly
    Article Snippet: .. Cre-mediated retrofitting was performed using 100 ng of self-ligated shuttle vector and 100 ng of expression vector in a 20 µL of reaction using 1 Unit of Cre recombinase (New England BioLabs) incubated at 37°C for 1 h. Ten microliters of the Cre reaction was then transformed into top 10 bacteria (Invitrogen) or electroporated into 10-beta Electrocompetent Escherichia coli (New England BioLabs). .. FLP recombination For removal of flippase recognition target (FRT)-flanked kanamycin, chloramphenicol, and spectinomycin cassettes, plasmids were transformed into Arabinose-induced flippase (FLP) recombinase-expressing bacteria EL250, as previously described ( ).

    Polymerase Chain Reaction:

    Article Title: Precise tuning of gene expression levels in mammalian cells
    Article Snippet: .. We purified the ligations using the QIAquick PCR Purification Kit (Qiagen) and transformed approximately 3.6 μg of the purified product into 10-beta Electrocompetent E.coli (NEB) following the manufacturer’s instructions. ..

    Gel Extraction:

    Article Title: Circular synthesized CRISPR/Cas gRNAs for functional interrogations in the coding and noncoding genome
    Article Snippet: .. Electroporation and I-SceI clean-up digest 2 mm cuvette (BTX, 45–0125), electrocompetent E. coli (10-beta, NEB, C3020K), SOC outgrowth medium (Thermo Fisher, 15544034), LB-media (Roth, X964.3) supplemented with 100 µg/ml ampicillin, Qiagen Plasmid Maxi Kit (Qiagen, 12163), I-SceI (NEB, R0694), NEB CutSmart buffer (NEB, B7204), 0.5% TAE/agarose gel, Thermo Fisher Scientific GeneJET Gel Extraction Kit. .. dU-ssDNA template amplification Bacteria (Escherichia coli strain K12 CJ236, NEB, E4141) were transformed with 500 ng of template plasmid according to the following protocol: DNA was mixed with 2 µl of 5x KCM buffer (0.5M KCl, 0.15M CaCl2 , 0.25M MgCl2 ) set to 10 µl with water and chilled on ice for 10 min. An equal volume of CJ236 bacteria was added to the DNA/KCM mixture, gently mixed, and incubated on ice for 15 min.

    Transformation Assay:

    Article Title: Predictable and precise template-free CRISPR editing of pathogenic variants
    Article Snippet: .. Assembled plasmids were purified by isopropanol precipitation with GlycoBlue Coprecipitant (Thermo Fisher) and reconstituted in milliQ water and transformed into NEB10beta (New England Biolabs) electrocompetent cells. .. Following recovery, a small dilution series was plated to assess transformation efficiency and the remainder was grown in liquid culture in DRM medium overnight at 37°C.

    Article Title: Minimized double guide RNA libraries enable scale-limited CRISPR/Cas9 screens
    Article Snippet: .. Reactions were pooled, column-purified and transformed in 14 electroporations (NEB 10-beta Electrocompetent E. coli C3020K). .. Bacterial cells were cultured overnight and plasmid DNA encoding an intermediate library was extracted using QIAGEN Plasmid Midi Kit (QIAGEN).

    Article Title: Harmonious genetic combinations rewire regulatory networks and flip gene essentiality
    Article Snippet: .. Clones were transformed into electrocompetent 10-Beta cells (New England Biolabs # C3020) using a 96-well plate electroporator using fresh electroporation plates (BTX 45–0450-M). .. Cells were recovered in deep-well plates (Thermo Scientific # 260252) and recovered in 0.6 ml SOC media for 1 h prior to inoculation into 0.6 ml LB + 100 mg/ml ampicillin.

    Article Title: Prioritizing disease and trait causal variants at the TNFAIP3 locus using functional and genomic features
    Article Snippet: .. Half of the ligated vector was then transformed into 100 μL of 10-beta e.coli (NEB, C3020K) by electroporation (2 kV, 200 Ω, 25 μF). .. Electroporated bacteria were immediately split into eight 1 mL aliquots of SOC (NEB, B9020S) and recovered for 1 h at 37 °C then independently expanded in 20 mL of LB supplemented with 100 μg/mL of carbenicillin (EMD, 69101-3) on a floor shaker at 37 °C for 6.5 h. After outgrowth aliquots were pooled prior to plasmid purification (QIAGEN, 12963).

    Article Title: Modular assembly of transposon integratable multigene vectors using RecWay assembly
    Article Snippet: .. Cre-mediated retrofitting was performed using 100 ng of self-ligated shuttle vector and 100 ng of expression vector in a 20 µL of reaction using 1 Unit of Cre recombinase (New England BioLabs) incubated at 37°C for 1 h. Ten microliters of the Cre reaction was then transformed into top 10 bacteria (Invitrogen) or electroporated into 10-beta Electrocompetent Escherichia coli (New England BioLabs). .. FLP recombination For removal of flippase recognition target (FRT)-flanked kanamycin, chloramphenicol, and spectinomycin cassettes, plasmids were transformed into Arabinose-induced flippase (FLP) recombinase-expressing bacteria EL250, as previously described ( ).

    Article Title: Precise tuning of gene expression levels in mammalian cells
    Article Snippet: .. We purified the ligations using the QIAquick PCR Purification Kit (Qiagen) and transformed approximately 3.6 μg of the purified product into 10-beta Electrocompetent E.coli (NEB) following the manufacturer’s instructions. ..

    Plasmid Preparation:

    Article Title: Prioritizing disease and trait causal variants at the TNFAIP3 locus using functional and genomic features
    Article Snippet: .. Half of the ligated vector was then transformed into 100 μL of 10-beta e.coli (NEB, C3020K) by electroporation (2 kV, 200 Ω, 25 μF). .. Electroporated bacteria were immediately split into eight 1 mL aliquots of SOC (NEB, B9020S) and recovered for 1 h at 37 °C then independently expanded in 20 mL of LB supplemented with 100 μg/mL of carbenicillin (EMD, 69101-3) on a floor shaker at 37 °C for 6.5 h. After outgrowth aliquots were pooled prior to plasmid purification (QIAGEN, 12963).

    Article Title: Modular assembly of transposon integratable multigene vectors using RecWay assembly
    Article Snippet: .. Cre-mediated retrofitting was performed using 100 ng of self-ligated shuttle vector and 100 ng of expression vector in a 20 µL of reaction using 1 Unit of Cre recombinase (New England BioLabs) incubated at 37°C for 1 h. Ten microliters of the Cre reaction was then transformed into top 10 bacteria (Invitrogen) or electroporated into 10-beta Electrocompetent Escherichia coli (New England BioLabs). .. FLP recombination For removal of flippase recognition target (FRT)-flanked kanamycin, chloramphenicol, and spectinomycin cassettes, plasmids were transformed into Arabinose-induced flippase (FLP) recombinase-expressing bacteria EL250, as previously described ( ).

    Article Title: Circular synthesized CRISPR/Cas gRNAs for functional interrogations in the coding and noncoding genome
    Article Snippet: .. Electroporation and I-SceI clean-up digest 2 mm cuvette (BTX, 45–0125), electrocompetent E. coli (10-beta, NEB, C3020K), SOC outgrowth medium (Thermo Fisher, 15544034), LB-media (Roth, X964.3) supplemented with 100 µg/ml ampicillin, Qiagen Plasmid Maxi Kit (Qiagen, 12163), I-SceI (NEB, R0694), NEB CutSmart buffer (NEB, B7204), 0.5% TAE/agarose gel, Thermo Fisher Scientific GeneJET Gel Extraction Kit. .. dU-ssDNA template amplification Bacteria (Escherichia coli strain K12 CJ236, NEB, E4141) were transformed with 500 ng of template plasmid according to the following protocol: DNA was mixed with 2 µl of 5x KCM buffer (0.5M KCl, 0.15M CaCl2 , 0.25M MgCl2 ) set to 10 µl with water and chilled on ice for 10 min. An equal volume of CJ236 bacteria was added to the DNA/KCM mixture, gently mixed, and incubated on ice for 15 min.

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    New England Biolabs dh10b electrocompetent cells
    Dh10b Electrocompetent Cells, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dh10b electrocompetent cells/product/New England Biolabs
    Average 99 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    dh10b electrocompetent cells - by Bioz Stars, 2020-08
    99/100 stars
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