1×q5 reaction buffer  (New England Biolabs)


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    Structured Review

    New England Biolabs 1×q5 reaction buffer
    1×Q5 Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1×q5 reaction buffer/product/New England Biolabs
    Average 87 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    1×q5 reaction buffer - by Bioz Stars, 2020-04
    87/100 stars

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    DNA Extraction:

    Article Title: Lentiviral Gag Assembly Analyzed through the Functional Characterization of Chimeric Simian Immunodeficiency Viruses Expressing Different Domains of the Feline Immunodeficiency Virus Capsid Protein
    Article Snippet: Paragraph title: DNA isolation and detection of viral replication intermediates (1-LTR circular form) ... The amplification reactions specific for the β-actin gene, included as an internal control for DNA quantitation and PCR efficiency, were carried out using 300 ng genomic DNA in 50-µl reactions containing 1×Q5 Reaction Buffer (New England Biolabs), 200 µM dNTPs, 0.5 µM each primer (5′-β-actin, 5′-CATGTGCAAGGCCGGCTTCGC-3′ and 3′-β-actin, 5′-CCTTAATGTCACGCACGATTTCC-3′ that target the first and third exon of the human β-actin gene, respectively), and 1 U Q5 High-Fidelity DNA Polymerase (New England Biolabs).

    Amplification:

    Article Title: Lentiviral Gag Assembly Analyzed through the Functional Characterization of Chimeric Simian Immunodeficiency Viruses Expressing Different Domains of the Feline Immunodeficiency Virus Capsid Protein
    Article Snippet: .. The amplification reactions specific for the β-actin gene, included as an internal control for DNA quantitation and PCR efficiency, were carried out using 300 ng genomic DNA in 50-µl reactions containing 1×Q5 Reaction Buffer (New England Biolabs), 200 µM dNTPs, 0.5 µM each primer (5′-β-actin, 5′-CATGTGCAAGGCCGGCTTCGC-3′ and 3′-β-actin, 5′-CCTTAATGTCACGCACGATTTCC-3′ that target the first and third exon of the human β-actin gene, respectively), and 1 U Q5 High-Fidelity DNA Polymerase (New England Biolabs). .. After an initial incubation step at 95 °C for 5 min, 25 amplification cycles were performed consisting of 20 s at 95 °C, 30 s at 55 °C and 25 s at 72 °C followed by a final elongation step at 72 °C for 2 min.

    Isolation:

    Article Title: Lentiviral Gag Assembly Analyzed through the Functional Characterization of Chimeric Simian Immunodeficiency Viruses Expressing Different Domains of the Feline Immunodeficiency Virus Capsid Protein
    Article Snippet: The amplification reactions specific for the β-actin gene, included as an internal control for DNA quantitation and PCR efficiency, were carried out using 300 ng genomic DNA in 50-µl reactions containing 1×Q5 Reaction Buffer (New England Biolabs), 200 µM dNTPs, 0.5 µM each primer (5′-β-actin, 5′-CATGTGCAAGGCCGGCTTCGC-3′ and 3′-β-actin, 5′-CCTTAATGTCACGCACGATTTCC-3′ that target the first and third exon of the human β-actin gene, respectively), and 1 U Q5 High-Fidelity DNA Polymerase (New England Biolabs). .. Low-molecular-weight DNA was isolated from the cell pellets employing the QIAprep Spin miniprep Kit (QIAGEN) and then used as template in amplification reactions.

    Quantitation Assay:

    Article Title: Lentiviral Gag Assembly Analyzed through the Functional Characterization of Chimeric Simian Immunodeficiency Viruses Expressing Different Domains of the Feline Immunodeficiency Virus Capsid Protein
    Article Snippet: .. The amplification reactions specific for the β-actin gene, included as an internal control for DNA quantitation and PCR efficiency, were carried out using 300 ng genomic DNA in 50-µl reactions containing 1×Q5 Reaction Buffer (New England Biolabs), 200 µM dNTPs, 0.5 µM each primer (5′-β-actin, 5′-CATGTGCAAGGCCGGCTTCGC-3′ and 3′-β-actin, 5′-CCTTAATGTCACGCACGATTTCC-3′ that target the first and third exon of the human β-actin gene, respectively), and 1 U Q5 High-Fidelity DNA Polymerase (New England Biolabs). .. After an initial incubation step at 95 °C for 5 min, 25 amplification cycles were performed consisting of 20 s at 95 °C, 30 s at 55 °C and 25 s at 72 °C followed by a final elongation step at 72 °C for 2 min.

    Purification:

    Article Title: Lentiviral Gag Assembly Analyzed through the Functional Characterization of Chimeric Simian Immunodeficiency Viruses Expressing Different Domains of the Feline Immunodeficiency Virus Capsid Protein
    Article Snippet: DNA isolation and detection of viral replication intermediates (1-LTR circular form) Total cellular DNA was purified using the illustra blood genomicPrep Mini Spin Kit (GE Life Sciences) as recommended by the manufacturer. .. The amplification reactions specific for the β-actin gene, included as an internal control for DNA quantitation and PCR efficiency, were carried out using 300 ng genomic DNA in 50-µl reactions containing 1×Q5 Reaction Buffer (New England Biolabs), 200 µM dNTPs, 0.5 µM each primer (5′-β-actin, 5′-CATGTGCAAGGCCGGCTTCGC-3′ and 3′-β-actin, 5′-CCTTAATGTCACGCACGATTTCC-3′ that target the first and third exon of the human β-actin gene, respectively), and 1 U Q5 High-Fidelity DNA Polymerase (New England Biolabs).

    Incubation:

    Article Title: Lentiviral Gag Assembly Analyzed through the Functional Characterization of Chimeric Simian Immunodeficiency Viruses Expressing Different Domains of the Feline Immunodeficiency Virus Capsid Protein
    Article Snippet: The amplification reactions specific for the β-actin gene, included as an internal control for DNA quantitation and PCR efficiency, were carried out using 300 ng genomic DNA in 50-µl reactions containing 1×Q5 Reaction Buffer (New England Biolabs), 200 µM dNTPs, 0.5 µM each primer (5′-β-actin, 5′-CATGTGCAAGGCCGGCTTCGC-3′ and 3′-β-actin, 5′-CCTTAATGTCACGCACGATTTCC-3′ that target the first and third exon of the human β-actin gene, respectively), and 1 U Q5 High-Fidelity DNA Polymerase (New England Biolabs). .. After an initial incubation step at 95 °C for 5 min, 25 amplification cycles were performed consisting of 20 s at 95 °C, 30 s at 55 °C and 25 s at 72 °C followed by a final elongation step at 72 °C for 2 min.

    Polymerase Chain Reaction:

    Article Title: Lentiviral Gag Assembly Analyzed through the Functional Characterization of Chimeric Simian Immunodeficiency Viruses Expressing Different Domains of the Feline Immunodeficiency Virus Capsid Protein
    Article Snippet: .. The amplification reactions specific for the β-actin gene, included as an internal control for DNA quantitation and PCR efficiency, were carried out using 300 ng genomic DNA in 50-µl reactions containing 1×Q5 Reaction Buffer (New England Biolabs), 200 µM dNTPs, 0.5 µM each primer (5′-β-actin, 5′-CATGTGCAAGGCCGGCTTCGC-3′ and 3′-β-actin, 5′-CCTTAATGTCACGCACGATTTCC-3′ that target the first and third exon of the human β-actin gene, respectively), and 1 U Q5 High-Fidelity DNA Polymerase (New England Biolabs). .. After an initial incubation step at 95 °C for 5 min, 25 amplification cycles were performed consisting of 20 s at 95 °C, 30 s at 55 °C and 25 s at 72 °C followed by a final elongation step at 72 °C for 2 min.

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    New England Biolabs reaction buffer
    Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 191 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reaction buffer/product/New England Biolabs
    Average 99 stars, based on 191 article reviews
    Price from $9.99 to $1999.99
    reaction buffer - by Bioz Stars, 2020-04
    99/100 stars
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