Journal: BMC Plant Biology

Article Title: Optimization of in vitro propagation and virus eradication using meristem culture and thermotherapy in two geranium species Pelargonium X hortorum (‘Zonal’) and Pelargonium × domesticum (‘Regal’)

doi: 10.1186/s12870-024-06027-y

Figure Lengend Snippet: RT-PCR amplification of virus specific fragments using degenerate primer IlarF5/IlarR7 for Bromoviridae. M: 1 kb size marker (GenRuler™ 1 kb DNA ladder, Fermentas). Numbers are as follow: 1: positive control Cucumber Mosaic Virus (CMV)-infected Peperomia magnifolia ; 2: negative control (healthy Peperomi magnifolia ); 3–13: Pelargonium samples

Article Snippet: M: GenRuler™ 1 kb DNA ladder (Fermentas); Lanes 1–3: Pelargonium samples; Lane 4: Negative control (plantlet derived from meristem tip culture of Pelargonium ), Lane 5: water, 6–8: PFBV specific primer on Pelargonium samples; Lane 9: negative control, Lane 10: water According to the results, bud sprouting of explants was significantly affected ( P ≤ 0.01) by species, medium, sampling time, and their interaction (Table ).

Techniques: Reverse Transcription Polymerase Chain Reaction, Amplification, Virus, Marker, Positive Control, Infection, Negative Control

Journal: BMC Plant Biology

Article Title: Optimization of in vitro propagation and virus eradication using meristem culture and thermotherapy in two geranium species Pelargonium X hortorum (‘Zonal’) and Pelargonium × domesticum (‘Regal’)

doi: 10.1186/s12870-024-06027-y

Figure Lengend Snippet: RT-PCR amplification of Tombusviridae family using universal primer of Tombusviridae family (CarmoIIF/CarmVIR) (Lanes 1–5) and specific primer for PFBV (CH2/CH1) (Lanes 6–10). M: GenRuler™ 1 kb DNA ladder (Fermentas); Lanes 1–3: Pelargonium samples; Lane 4: Negative control (plantlet derived from meristem tip culture of Pelargonium ), Lane 5: water, 6–8: PFBV specific primer on Pelargonium samples; Lane 9: negative control, Lane 10: water

Article Snippet: M: GenRuler™ 1 kb DNA ladder (Fermentas); Lanes 1–3: Pelargonium samples; Lane 4: Negative control (plantlet derived from meristem tip culture of Pelargonium ), Lane 5: water, 6–8: PFBV specific primer on Pelargonium samples; Lane 9: negative control, Lane 10: water According to the results, bud sprouting of explants was significantly affected ( P ≤ 0.01) by species, medium, sampling time, and their interaction (Table ).

Techniques: Reverse Transcription Polymerase Chain Reaction, Amplification, Negative Control, Derivative Assay

Journal: BMC Plant Biology

Article Title: Optimization of in vitro propagation and virus eradication using meristem culture and thermotherapy in two geranium species Pelargonium X hortorum (‘Zonal’) and Pelargonium × domesticum (‘Regal’)

doi: 10.1186/s12870-024-06027-y

Figure Lengend Snippet: Detection and confirmation of Tombusviridae and PFBV virus elimination using heat treatment and meristem tip culture in five geranium plantlets. Left: Tombusviridae virus detection in geranium samples before (P19, P21, P108, P111, P112) and after (MTCP19, MTCP21, MTCP108, MTCP111, MTCP112) heat treatment and meristem tip culture. Right: PFBV virus detection in geranium samples before (P19, P21, P108, P111, P112) and after (MTCP19, MTCP21, MTCP108, MTCP111, MTCP112) heat treatment and meristem tip culture. M: 1 kb size marker (GenRuler™ 1 kb DNA ladder, Fermentas)

Article Snippet: M: GenRuler™ 1 kb DNA ladder (Fermentas); Lanes 1–3: Pelargonium samples; Lane 4: Negative control (plantlet derived from meristem tip culture of Pelargonium ), Lane 5: water, 6–8: PFBV specific primer on Pelargonium samples; Lane 9: negative control, Lane 10: water According to the results, bud sprouting of explants was significantly affected ( P ≤ 0.01) by species, medium, sampling time, and their interaction (Table ).

Techniques: Virus, Marker

Journal: Scientific Reports

Article Title: Continuous cell lines derived from the Asian citrus psyllid, Diaphorina citri, harbor viruses and Wolbachia

doi: 10.1038/s41598-024-83671-2

Figure Lengend Snippet: PCR of Wolbachia ftsz and wsp confirms the presence of Wolbachia in Dici1 and Dici5, but not Dici3 cells. ( A ) Partial fragments of Wolbachia ftsz (1043 bp) and wsp (570 bp) were amplified from Dici1 (Passage 27, GenBank: PP894982 for ftsz and PP894984 for wsp ) and Dici5 (Passage 33, GenBank: PP894983 for ftsz and PP894985 for wsp ), but not Dici3 (Passage 46), genomic DNA. NTC: no template control. Arrows indicate PCR products at the expected sizes. GeneRuler 1 kb plus DNA ladder (ThermoFisher Scientific, SM1332) was used as a DNA marker. Two weeks of doxycycline treatment reduced the levels of ftsz and wsp in Dici1 ( B ) and Dici5 ( C ) cells.

Article Snippet: GeneRuler 1 kb plus DNA ladder (ThermoFisher Scientific, SM1332) was used as a DNA marker.

Techniques: Amplification, Control, Marker

Journal: Scientific Reports

Article Title: Continuous cell lines derived from the Asian citrus psyllid, Diaphorina citri, harbor viruses and Wolbachia

doi: 10.1038/s41598-024-83671-2

Figure Lengend Snippet: Evaluation of viral infection status in Diaphorina citri cell lines. ( A ) DcRV RT-PCR confirms the presence of this reovirus in Dici1, but not Dici3 cells. Partial fragments (900 bp) of the DcRV p8 were amplified from the cDNA of Dici1 (P1, GenBank: PP894986), but not Dici3 (P5). ( B ) RT-PCR confirmed the presence of DcACV and DcRV in Dici5 cells. Partial fragments of the DcACV RNA1 (473 bp, GenBank: PP894988) and DcRV p8 (GenBank: PP894987) were amplified from the cDNA of Dici5 (Passage 33). GeneRuler 1 kb plus DNA ladder was used as a DNA marker. + control: cDNA made from purified D. citri viruses. NTC: no template control. Arrows indicate PCR products at the expected sizes.

Article Snippet: GeneRuler 1 kb plus DNA ladder (ThermoFisher Scientific, SM1332) was used as a DNA marker.

Techniques: Infection, Reverse Transcription Polymerase Chain Reaction, Amplification, Marker, Control, Purification