Journal: bioRxiv
Article Title: Identification and receptor mechanism of TIR-catalyzed small molecules in plant immunity
doi: 10.1101/2022.04.01.486681
Figure Lengend Snippet: Reagents and conditions: a) 1,3-dichloro-1,1,3,3-tetraisopropyldisiloxane, pyridine, overnight, 75%; b) beta-D-ribofuranose 1-acetate 2,3,5-tribenzoate, SnCl 4 , DCE, 30 h, 70%; c) TBAF, THF, 30 min, 80%; d) DMTrCl, pyridine, 2 h; e) TBSCl, imidazole, 2 h, two-step yield 61%; f) PTSA, CHCl 3 /MeOH, 10 min, 91%; g) dibenzyl diisopropylphosphoramidite, 4,5-dicyanoimidazole, TBHP, CH 2 Cl 2 /MeCN, 4.5 h, 71%; h) NH 3 /MeOH, 24 h, 80%; i) DMTrCl, pyridine, 2 h; j) TBSCl, imidazole, 2 h; k) p -toluenesulfonic acid monohydrate, CHCl 3 /MeOH, 10 min, three-step yield 58%; l) dibenzyl diisopropylphosphoramidite, 4,5-dicyanoimidazole, TBHP, CH 2 Cl 2 /MeCN, 4.5 h, 69%; m) TBAF, THF, 30 min; n) Pd/C, Et 3 N, H 2 , t -BuOH/H 2 O, two-step yield 50%.
Article Snippet: The mass spectra (MS, Thermo, LCQ, Fleet) were measured at the School of Pharmaceutical Sciences, Zhengzhou University Compound 2: To a stirring solution of 1 (30.00 g, 80.84 mmol) in pyridine (200 mL) at 0 °C was added 1,3-dichloro-1,1,3,3-tetraisopropyldisiloxane (26.77 g, 84.88 mmol), and the solution was stirred at room temperature for overnight.
Techniques: