mouse anti nf200  (Valiant)

 
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    Name:
    anti human neurofilament 200 mouse monoclonal antibody
    Description:
    The immunogen used to produce this monoclonal antibody is neurofilament purified from human brain The isotype is mouse IgG1 This product is a monoclonal antibody to the neurofilament peptide of apparent molecular weight of 200 000
    Catalog Number:
    08697051
    Price:
    421.6
    Category:
    Life Sciences Antibodies Primary Antibodies
    Applications:
    Immunohistochemistry, Immunocytochemistry ,Immunoblot, Immunoprecipitation
    Size:
    100 µL
    Buy from Supplier


    Structured Review

    Valiant mouse anti nf200
    The immunogen used to produce this monoclonal antibody is neurofilament purified from human brain The isotype is mouse IgG1 This product is a monoclonal antibody to the neurofilament peptide of apparent molecular weight of 200 000
    https://www.bioz.com/result/mouse anti nf200/product/Valiant
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti nf200 - by Bioz Stars, 2021-04
    88/100 stars

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    Related Articles

    Immunostaining:

    Article Title: Type II Cochlear Ganglion Neurons Do Not Drive the Olivocochlear Reflex: Re-Examination of the Cochlear Phenotype in Peripherin Knock-Out Mice
    Article Snippet: .. Pieces were permeabilized with a freeze/thaw cycle, as follows: cryoprotected in 30% sucrose for 15 min, frozen on dry ice, thawed, and rinsed in PBS for 15 min. Immunostaining began with a blocking buffer (PBS with 5% normal horse serum and 0.3% Triton X-100) for 1 h at room temperature and was followed by overnight incubation at 37ºC with some combination of the following primary antibodies: (1) rabbit anti-peripherin (catalog #ab4666, Abcam) at 1:200; (2) goat anti-Na+ /K+ -ATPase α3 (C-16; catalog #sc-16052, Santa Cruz Biotechnology) at 1:200 to label type I afferents and MOC efferents; (3) chicken anti-NF-H (neurofilament; catalog #AB5539, Chemicon) at 1:1000, or mouse anti-NF200 (catalog #69705, MP Biomedicals) at 1:50,000, or mouse anti-TuJ1 (β-tubulin III; catalog #MMS-435P, Covance) at 1:2000 to label cochlear afferent and efferent fibers; (4) goat anti-parvalbumin (catalog #PVG-214, Swant) at 1:2000, to delineate type II outer spiral fibers and their terminal swellings; (5) mouse anti-synaptophysin (catalog #69730, MP Biomedicals) at 1:100, or rabbit anti-VAT [vesicular acetylcholine (ACh) transporter; catalog #ab68986, Abcam] at 1:200, to label terminals of cochlear efferent fibers; (6) mouse anti-CtBP2 (C-terminal binding protein; catalog #612044, BD Biosciences) at 1:200, to quantify presynaptic ribbons; and/or (7) rabbit anti-myosin VIIa (catalog #25-6790, Proteus Biosciences) at 1:200 to delineate the hair cell cytoplasm. ..

    Blocking Assay:

    Article Title: Type II Cochlear Ganglion Neurons Do Not Drive the Olivocochlear Reflex: Re-Examination of the Cochlear Phenotype in Peripherin Knock-Out Mice
    Article Snippet: .. Pieces were permeabilized with a freeze/thaw cycle, as follows: cryoprotected in 30% sucrose for 15 min, frozen on dry ice, thawed, and rinsed in PBS for 15 min. Immunostaining began with a blocking buffer (PBS with 5% normal horse serum and 0.3% Triton X-100) for 1 h at room temperature and was followed by overnight incubation at 37ºC with some combination of the following primary antibodies: (1) rabbit anti-peripherin (catalog #ab4666, Abcam) at 1:200; (2) goat anti-Na+ /K+ -ATPase α3 (C-16; catalog #sc-16052, Santa Cruz Biotechnology) at 1:200 to label type I afferents and MOC efferents; (3) chicken anti-NF-H (neurofilament; catalog #AB5539, Chemicon) at 1:1000, or mouse anti-NF200 (catalog #69705, MP Biomedicals) at 1:50,000, or mouse anti-TuJ1 (β-tubulin III; catalog #MMS-435P, Covance) at 1:2000 to label cochlear afferent and efferent fibers; (4) goat anti-parvalbumin (catalog #PVG-214, Swant) at 1:2000, to delineate type II outer spiral fibers and their terminal swellings; (5) mouse anti-synaptophysin (catalog #69730, MP Biomedicals) at 1:100, or rabbit anti-VAT [vesicular acetylcholine (ACh) transporter; catalog #ab68986, Abcam] at 1:200, to label terminals of cochlear efferent fibers; (6) mouse anti-CtBP2 (C-terminal binding protein; catalog #612044, BD Biosciences) at 1:200, to quantify presynaptic ribbons; and/or (7) rabbit anti-myosin VIIa (catalog #25-6790, Proteus Biosciences) at 1:200 to delineate the hair cell cytoplasm. ..

    Incubation:

    Article Title: Type II Cochlear Ganglion Neurons Do Not Drive the Olivocochlear Reflex: Re-Examination of the Cochlear Phenotype in Peripherin Knock-Out Mice
    Article Snippet: .. Pieces were permeabilized with a freeze/thaw cycle, as follows: cryoprotected in 30% sucrose for 15 min, frozen on dry ice, thawed, and rinsed in PBS for 15 min. Immunostaining began with a blocking buffer (PBS with 5% normal horse serum and 0.3% Triton X-100) for 1 h at room temperature and was followed by overnight incubation at 37ºC with some combination of the following primary antibodies: (1) rabbit anti-peripherin (catalog #ab4666, Abcam) at 1:200; (2) goat anti-Na+ /K+ -ATPase α3 (C-16; catalog #sc-16052, Santa Cruz Biotechnology) at 1:200 to label type I afferents and MOC efferents; (3) chicken anti-NF-H (neurofilament; catalog #AB5539, Chemicon) at 1:1000, or mouse anti-NF200 (catalog #69705, MP Biomedicals) at 1:50,000, or mouse anti-TuJ1 (β-tubulin III; catalog #MMS-435P, Covance) at 1:2000 to label cochlear afferent and efferent fibers; (4) goat anti-parvalbumin (catalog #PVG-214, Swant) at 1:2000, to delineate type II outer spiral fibers and their terminal swellings; (5) mouse anti-synaptophysin (catalog #69730, MP Biomedicals) at 1:100, or rabbit anti-VAT [vesicular acetylcholine (ACh) transporter; catalog #ab68986, Abcam] at 1:200, to label terminals of cochlear efferent fibers; (6) mouse anti-CtBP2 (C-terminal binding protein; catalog #612044, BD Biosciences) at 1:200, to quantify presynaptic ribbons; and/or (7) rabbit anti-myosin VIIa (catalog #25-6790, Proteus Biosciences) at 1:200 to delineate the hair cell cytoplasm. ..

    Binding Assay:

    Article Title: Type II Cochlear Ganglion Neurons Do Not Drive the Olivocochlear Reflex: Re-Examination of the Cochlear Phenotype in Peripherin Knock-Out Mice
    Article Snippet: .. Pieces were permeabilized with a freeze/thaw cycle, as follows: cryoprotected in 30% sucrose for 15 min, frozen on dry ice, thawed, and rinsed in PBS for 15 min. Immunostaining began with a blocking buffer (PBS with 5% normal horse serum and 0.3% Triton X-100) for 1 h at room temperature and was followed by overnight incubation at 37ºC with some combination of the following primary antibodies: (1) rabbit anti-peripherin (catalog #ab4666, Abcam) at 1:200; (2) goat anti-Na+ /K+ -ATPase α3 (C-16; catalog #sc-16052, Santa Cruz Biotechnology) at 1:200 to label type I afferents and MOC efferents; (3) chicken anti-NF-H (neurofilament; catalog #AB5539, Chemicon) at 1:1000, or mouse anti-NF200 (catalog #69705, MP Biomedicals) at 1:50,000, or mouse anti-TuJ1 (β-tubulin III; catalog #MMS-435P, Covance) at 1:2000 to label cochlear afferent and efferent fibers; (4) goat anti-parvalbumin (catalog #PVG-214, Swant) at 1:2000, to delineate type II outer spiral fibers and their terminal swellings; (5) mouse anti-synaptophysin (catalog #69730, MP Biomedicals) at 1:100, or rabbit anti-VAT [vesicular acetylcholine (ACh) transporter; catalog #ab68986, Abcam] at 1:200, to label terminals of cochlear efferent fibers; (6) mouse anti-CtBP2 (C-terminal binding protein; catalog #612044, BD Biosciences) at 1:200, to quantify presynaptic ribbons; and/or (7) rabbit anti-myosin VIIa (catalog #25-6790, Proteus Biosciences) at 1:200 to delineate the hair cell cytoplasm. ..

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  • 88
    Valiant mouse anti nf200
    Mouse Anti Nf200, supplied by Valiant, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti nf200/product/Valiant
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti nf200 - by Bioz Stars, 2021-04
    88/100 stars
      Buy from Supplier

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