anti vinculin  (Valiant)

 
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    Name:
    Anti vinculin mouse monoclonal antibody
    Description:
    Anti Human Clone FB11 Isotype mouse IgG 1 Applications Immunohistochemistry Immunoblotting
    Catalog Number:
    08693291
    Price:
    289.45
    Category:
    Life Sciences Antibodies Primary Antibodies
    Applications:
    Immunohistochemistry ,Immunoblot, Immunofluorescence assays (IFA)
    Size:
    10 µg
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    Structured Review

    Valiant anti vinculin
    Generation and validation of TNS1-KO MDCK cells. a Schematic diagram of the generation of TNS1-KO cells by using two vectors CRISPR/Cas9 and homology-directed repair technology. The guide plasmid containing the TNS1 target sequence and Cas9 coding sequence and the donor vector were co-transfected into cells. The guide plasmid created a double-stranded break at the target site of TNS1. The donor vector contains about 600 bp homologous to the 5′ and 3′ flanking region of exon1 and is used as template for DNA repair by homologous recombination, resulting in replacement of TNS1 exon1, which contains the first ATG site, with the donor cassette that includes promoter-less GFP, PGK promotor and the puromycin selection gene flanked with loxP sites. MDCK WT or KO cells were lysed or fixed for immunoblotting ( b ), RT-PCR ( c ), or immunofluorescence ( d ) assays, respectively. Immunoblotting by TNS1 antibody detected 220kD TNS1 protein in WT but not any of the four KO clones. KO1 clone was used for further analysis. TNS1 mRNA was only presented in WT cells. Immunofluorescence staining showed co-localizations of TNS1 and <t>vinculin</t> at focal adhesion sites (arrows) in WT but not in KO cells. Nuclear stainings shown in WT and KO1 with TNS1 antibody were likely due to the secondary antibody. Scale bar = 10 μm.
    Anti Human Clone FB11 Isotype mouse IgG 1 Applications Immunohistochemistry Immunoblotting
    https://www.bioz.com/result/anti vinculin/product/Valiant
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti vinculin - by Bioz Stars, 2021-04
    92/100 stars

    Images

    1) Product Images from "Hyperactivity of Mek in TNS1 knockouts leads to potential treatments for cystic kidney diseases"

    Article Title: Hyperactivity of Mek in TNS1 knockouts leads to potential treatments for cystic kidney diseases

    Journal: Cell Death & Disease

    doi: 10.1038/s41419-019-2119-7

    Generation and validation of TNS1-KO MDCK cells. a Schematic diagram of the generation of TNS1-KO cells by using two vectors CRISPR/Cas9 and homology-directed repair technology. The guide plasmid containing the TNS1 target sequence and Cas9 coding sequence and the donor vector were co-transfected into cells. The guide plasmid created a double-stranded break at the target site of TNS1. The donor vector contains about 600 bp homologous to the 5′ and 3′ flanking region of exon1 and is used as template for DNA repair by homologous recombination, resulting in replacement of TNS1 exon1, which contains the first ATG site, with the donor cassette that includes promoter-less GFP, PGK promotor and the puromycin selection gene flanked with loxP sites. MDCK WT or KO cells were lysed or fixed for immunoblotting ( b ), RT-PCR ( c ), or immunofluorescence ( d ) assays, respectively. Immunoblotting by TNS1 antibody detected 220kD TNS1 protein in WT but not any of the four KO clones. KO1 clone was used for further analysis. TNS1 mRNA was only presented in WT cells. Immunofluorescence staining showed co-localizations of TNS1 and vinculin at focal adhesion sites (arrows) in WT but not in KO cells. Nuclear stainings shown in WT and KO1 with TNS1 antibody were likely due to the secondary antibody. Scale bar = 10 μm.
    Figure Legend Snippet: Generation and validation of TNS1-KO MDCK cells. a Schematic diagram of the generation of TNS1-KO cells by using two vectors CRISPR/Cas9 and homology-directed repair technology. The guide plasmid containing the TNS1 target sequence and Cas9 coding sequence and the donor vector were co-transfected into cells. The guide plasmid created a double-stranded break at the target site of TNS1. The donor vector contains about 600 bp homologous to the 5′ and 3′ flanking region of exon1 and is used as template for DNA repair by homologous recombination, resulting in replacement of TNS1 exon1, which contains the first ATG site, with the donor cassette that includes promoter-less GFP, PGK promotor and the puromycin selection gene flanked with loxP sites. MDCK WT or KO cells were lysed or fixed for immunoblotting ( b ), RT-PCR ( c ), or immunofluorescence ( d ) assays, respectively. Immunoblotting by TNS1 antibody detected 220kD TNS1 protein in WT but not any of the four KO clones. KO1 clone was used for further analysis. TNS1 mRNA was only presented in WT cells. Immunofluorescence staining showed co-localizations of TNS1 and vinculin at focal adhesion sites (arrows) in WT but not in KO cells. Nuclear stainings shown in WT and KO1 with TNS1 antibody were likely due to the secondary antibody. Scale bar = 10 μm.

    Techniques Used: CRISPR, Plasmid Preparation, Sequencing, Transfection, Homologous Recombination, Selection, Reverse Transcription Polymerase Chain Reaction, Immunofluorescence, Clone Assay, Staining

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  • 92
    Valiant anti vinculin
    Generation and validation of TNS1-KO MDCK cells. a Schematic diagram of the generation of TNS1-KO cells by using two vectors CRISPR/Cas9 and homology-directed repair technology. The guide plasmid containing the TNS1 target sequence and Cas9 coding sequence and the donor vector were co-transfected into cells. The guide plasmid created a double-stranded break at the target site of TNS1. The donor vector contains about 600 bp homologous to the 5′ and 3′ flanking region of exon1 and is used as template for DNA repair by homologous recombination, resulting in replacement of TNS1 exon1, which contains the first ATG site, with the donor cassette that includes promoter-less GFP, PGK promotor and the puromycin selection gene flanked with loxP sites. MDCK WT or KO cells were lysed or fixed for immunoblotting ( b ), RT-PCR ( c ), or immunofluorescence ( d ) assays, respectively. Immunoblotting by TNS1 antibody detected 220kD TNS1 protein in WT but not any of the four KO clones. KO1 clone was used for further analysis. TNS1 mRNA was only presented in WT cells. Immunofluorescence staining showed co-localizations of TNS1 and <t>vinculin</t> at focal adhesion sites (arrows) in WT but not in KO cells. Nuclear stainings shown in WT and KO1 with TNS1 antibody were likely due to the secondary antibody. Scale bar = 10 μm.
    Anti Vinculin, supplied by Valiant, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti vinculin/product/Valiant
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti vinculin - by Bioz Stars, 2021-04
    92/100 stars
      Buy from Supplier

    Image Search Results


    Generation and validation of TNS1-KO MDCK cells. a Schematic diagram of the generation of TNS1-KO cells by using two vectors CRISPR/Cas9 and homology-directed repair technology. The guide plasmid containing the TNS1 target sequence and Cas9 coding sequence and the donor vector were co-transfected into cells. The guide plasmid created a double-stranded break at the target site of TNS1. The donor vector contains about 600 bp homologous to the 5′ and 3′ flanking region of exon1 and is used as template for DNA repair by homologous recombination, resulting in replacement of TNS1 exon1, which contains the first ATG site, with the donor cassette that includes promoter-less GFP, PGK promotor and the puromycin selection gene flanked with loxP sites. MDCK WT or KO cells were lysed or fixed for immunoblotting ( b ), RT-PCR ( c ), or immunofluorescence ( d ) assays, respectively. Immunoblotting by TNS1 antibody detected 220kD TNS1 protein in WT but not any of the four KO clones. KO1 clone was used for further analysis. TNS1 mRNA was only presented in WT cells. Immunofluorescence staining showed co-localizations of TNS1 and vinculin at focal adhesion sites (arrows) in WT but not in KO cells. Nuclear stainings shown in WT and KO1 with TNS1 antibody were likely due to the secondary antibody. Scale bar = 10 μm.

    Journal: Cell Death & Disease

    Article Title: Hyperactivity of Mek in TNS1 knockouts leads to potential treatments for cystic kidney diseases

    doi: 10.1038/s41419-019-2119-7

    Figure Lengend Snippet: Generation and validation of TNS1-KO MDCK cells. a Schematic diagram of the generation of TNS1-KO cells by using two vectors CRISPR/Cas9 and homology-directed repair technology. The guide plasmid containing the TNS1 target sequence and Cas9 coding sequence and the donor vector were co-transfected into cells. The guide plasmid created a double-stranded break at the target site of TNS1. The donor vector contains about 600 bp homologous to the 5′ and 3′ flanking region of exon1 and is used as template for DNA repair by homologous recombination, resulting in replacement of TNS1 exon1, which contains the first ATG site, with the donor cassette that includes promoter-less GFP, PGK promotor and the puromycin selection gene flanked with loxP sites. MDCK WT or KO cells were lysed or fixed for immunoblotting ( b ), RT-PCR ( c ), or immunofluorescence ( d ) assays, respectively. Immunoblotting by TNS1 antibody detected 220kD TNS1 protein in WT but not any of the four KO clones. KO1 clone was used for further analysis. TNS1 mRNA was only presented in WT cells. Immunofluorescence staining showed co-localizations of TNS1 and vinculin at focal adhesion sites (arrows) in WT but not in KO cells. Nuclear stainings shown in WT and KO1 with TNS1 antibody were likely due to the secondary antibody. Scale bar = 10 μm.

    Article Snippet: Anti-Vinculin (#693291) was from MP Biomedical.

    Techniques: CRISPR, Plasmid Preparation, Sequencing, Transfection, Homologous Recombination, Selection, Reverse Transcription Polymerase Chain Reaction, Immunofluorescence, Clone Assay, Staining