anti rat igg antibodies  (Valiant)

 
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    Name:
    Anti rat IgG H L affinity purified mouse antibody
    Description:
    Sterile liquid This product is supplied in 0 01M Sodium Phosphate 0 25M NaCl pH 7 6 This product contains no preservatives
    Catalog Number:
    08670441
    Price:
    386.95
    Category:
    Life Sciences Antibodies Secondary Antibodies
    Applications:
    Immunoassays, Immunohistochemistry
    Size:
    2 mg
    Buy from Supplier


    Structured Review

    Valiant anti rat igg antibodies
    Association of SV40 Vp1 with cellular/viral (co)chaperones during SV40 infection. (A) The RIPA lysate prepared from 10 7 SV40-infected TC7 cells at 50 h postinfection was immunoprecipitated with affinity-purified anti-Vp1 <t>IgG</t> and analyzed for the presence of LT, Hsc70, Hsp70, or PDI by Western blotting. As a control, the same number of uninfected COS-7 cells was also analyzed. For input, 1/480 of the lysate used for immunoreactions was loaded. PDI and an immunoglobulin heavy chain (Ig) were detected with anti-PDI antibody (arrow). (B) The RIPA lysate from 10 6 infected cells was immunoprecipitated with antibodies against β-Gal, Hsc70, Hsp70, a unique LT epitope, a unique ST epitope, and Hsp40, and the IPs were probed for Vp1 by Western blotting. One-thousandth of the input lysate was analyzed for Vp1 in the same blot. (C) The Sol fraction from 10 6 infected cells, which contains endogenous and infection-based Vp1, was mixed with two recombinant Vp1 proteins, Vp1ΔC58-His6 pentamer (ΔC) and GST-Vp1C69 (C69). After IP with antibodies against β-Gal, Hsc70, Hsp70, Hsp40, a unique LT epitope, a unique ST epitope, and PDI, the IPs and 1/1,000 of the inputs were analyzed by anti-Vp1 Western blotting. The reactions shown in lanes 4, 5, and 10, to 12 were performed using <t>TrueBlot</t> anti-mouse beads. (D) The Csk fraction prepared from 2.5 × 10 6 infected cells was immunoprecipitated with antibodies against β-Gal, Hsc70, or a unique LT epitope, and Vp1 in the IPs was analyzed by anti-Vp1 Western blotting. Vp1 in 1/1,000 of the input was also analyzed. (E) The Sol fraction from 10 4 infected cells or the Csk fraction from 2.5 × 10 4 infected cells was analyzed for Hsc70, Hsp70, and LT by Western blotting.
    Sterile liquid This product is supplied in 0 01M Sodium Phosphate 0 25M NaCl pH 7 6 This product contains no preservatives
    https://www.bioz.com/result/anti rat igg antibodies/product/Valiant
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti rat igg antibodies - by Bioz Stars, 2021-04
    90/100 stars

    Images

    1) Product Images from "Association of Simian Virus 40 Vp1 with 70-Kilodalton Heat Shock Proteins and Viral Tumor Antigens ▿"

    Article Title: Association of Simian Virus 40 Vp1 with 70-Kilodalton Heat Shock Proteins and Viral Tumor Antigens ▿

    Journal: Journal of Virology

    doi: 10.1128/JVI.00844-08

    Association of SV40 Vp1 with cellular/viral (co)chaperones during SV40 infection. (A) The RIPA lysate prepared from 10 7 SV40-infected TC7 cells at 50 h postinfection was immunoprecipitated with affinity-purified anti-Vp1 IgG and analyzed for the presence of LT, Hsc70, Hsp70, or PDI by Western blotting. As a control, the same number of uninfected COS-7 cells was also analyzed. For input, 1/480 of the lysate used for immunoreactions was loaded. PDI and an immunoglobulin heavy chain (Ig) were detected with anti-PDI antibody (arrow). (B) The RIPA lysate from 10 6 infected cells was immunoprecipitated with antibodies against β-Gal, Hsc70, Hsp70, a unique LT epitope, a unique ST epitope, and Hsp40, and the IPs were probed for Vp1 by Western blotting. One-thousandth of the input lysate was analyzed for Vp1 in the same blot. (C) The Sol fraction from 10 6 infected cells, which contains endogenous and infection-based Vp1, was mixed with two recombinant Vp1 proteins, Vp1ΔC58-His6 pentamer (ΔC) and GST-Vp1C69 (C69). After IP with antibodies against β-Gal, Hsc70, Hsp70, Hsp40, a unique LT epitope, a unique ST epitope, and PDI, the IPs and 1/1,000 of the inputs were analyzed by anti-Vp1 Western blotting. The reactions shown in lanes 4, 5, and 10, to 12 were performed using TrueBlot anti-mouse beads. (D) The Csk fraction prepared from 2.5 × 10 6 infected cells was immunoprecipitated with antibodies against β-Gal, Hsc70, or a unique LT epitope, and Vp1 in the IPs was analyzed by anti-Vp1 Western blotting. Vp1 in 1/1,000 of the input was also analyzed. (E) The Sol fraction from 10 4 infected cells or the Csk fraction from 2.5 × 10 4 infected cells was analyzed for Hsc70, Hsp70, and LT by Western blotting.
    Figure Legend Snippet: Association of SV40 Vp1 with cellular/viral (co)chaperones during SV40 infection. (A) The RIPA lysate prepared from 10 7 SV40-infected TC7 cells at 50 h postinfection was immunoprecipitated with affinity-purified anti-Vp1 IgG and analyzed for the presence of LT, Hsc70, Hsp70, or PDI by Western blotting. As a control, the same number of uninfected COS-7 cells was also analyzed. For input, 1/480 of the lysate used for immunoreactions was loaded. PDI and an immunoglobulin heavy chain (Ig) were detected with anti-PDI antibody (arrow). (B) The RIPA lysate from 10 6 infected cells was immunoprecipitated with antibodies against β-Gal, Hsc70, Hsp70, a unique LT epitope, a unique ST epitope, and Hsp40, and the IPs were probed for Vp1 by Western blotting. One-thousandth of the input lysate was analyzed for Vp1 in the same blot. (C) The Sol fraction from 10 6 infected cells, which contains endogenous and infection-based Vp1, was mixed with two recombinant Vp1 proteins, Vp1ΔC58-His6 pentamer (ΔC) and GST-Vp1C69 (C69). After IP with antibodies against β-Gal, Hsc70, Hsp70, Hsp40, a unique LT epitope, a unique ST epitope, and PDI, the IPs and 1/1,000 of the inputs were analyzed by anti-Vp1 Western blotting. The reactions shown in lanes 4, 5, and 10, to 12 were performed using TrueBlot anti-mouse beads. (D) The Csk fraction prepared from 2.5 × 10 6 infected cells was immunoprecipitated with antibodies against β-Gal, Hsc70, or a unique LT epitope, and Vp1 in the IPs was analyzed by anti-Vp1 Western blotting. Vp1 in 1/1,000 of the input was also analyzed. (E) The Sol fraction from 10 4 infected cells or the Csk fraction from 2.5 × 10 4 infected cells was analyzed for Hsc70, Hsp70, and LT by Western blotting.

    Techniques Used: Infection, Immunoprecipitation, Affinity Purification, Western Blot, Recombinant

    Related Articles

    Purification:

    Article Title: Targeting IL-6 by both passive or active immunization strategies prevents bleomycin-induced skin fibrosis
    Article Snippet: Anti-IL-6 receptor monoclonal antibody treatment Rat anti-mouse IL-6 receptor monoclonal antibody (clone MR16-1) described previously was provided by Chugai Pharmaceutical (Tokyo, Japan) [ , ]. .. Purified rat IgG1 (isotype-matched control antibody, MP Biomedicals, Illkirch, France) was administered as a negative control. .. DBA/2 mice received, in parallel of bleomycin injections, an intraperitoneal (i.p.) injection of 2 mg at day 0 followed by one i.p. injection of 1 mg at days 7 and 14.

    Negative Control:

    Article Title: Targeting IL-6 by both passive or active immunization strategies prevents bleomycin-induced skin fibrosis
    Article Snippet: Anti-IL-6 receptor monoclonal antibody treatment Rat anti-mouse IL-6 receptor monoclonal antibody (clone MR16-1) described previously was provided by Chugai Pharmaceutical (Tokyo, Japan) [ , ]. .. Purified rat IgG1 (isotype-matched control antibody, MP Biomedicals, Illkirch, France) was administered as a negative control. .. DBA/2 mice received, in parallel of bleomycin injections, an intraperitoneal (i.p.) injection of 2 mg at day 0 followed by one i.p. injection of 1 mg at days 7 and 14.

    Incubation:

    Article Title: Simultaneous Detection of Antibodies to Mouse Hepatitis Virus Recombinant Structural Proteins by a Microsphere-Based Multiplex Fluorescence Immunoassay ▿
    Article Snippet: Aliquots of 10 μl of test sera at a dilution of 1:10 in PBS were added to each well and incubated at 37°C for 15 min. .. The slides were then washed in PBS and incubated with 10 μl of fluorescein-conjugated anti-mouse IgG (MP Biomedicals, Irvine, CA) or fluorescein-conjugated anti-rat IgG (MP Biomedicals) diluted 1:100 in PBS containing 1% Evan's blue dye at 37°C for 15 min. After the slides were washed, specific fluorescence was analyzed using a UV epifluorescence microscope. .. The complete nucleotide sequence of the S gene cloned from the MHV-S strain has been submitted to DDBJ/EMBL/GenBank under accession no. .

    Article Title: K+ modulates genetic competence and the stress regulon of Streptococcus mutans
    Article Snippet: Following electrophoresis, separated proteins were blotted onto PVDF membrane for 7 min using the Transblot Turbo (BioRad) and membranes were blocked overnight in PBS 0.3 %, Tween 20 5 % skimmed milk. .. Primary murine ascites fluid, rat antiserum or rabbit antiserum was added to the blot at a 1 : 500 dilution and incubated for 1 h at room temperature, and 1 : 1000 goat-anti-mouse, anti-rat or anti-rabbit-HRP conjugated antibody was added (MP Biomedicals). .. The blots were treated with ECL-Prime substrate for 1 min prior to chemical imaging in the G-Box (Syngene).

    Fluorescence:

    Article Title: Simultaneous Detection of Antibodies to Mouse Hepatitis Virus Recombinant Structural Proteins by a Microsphere-Based Multiplex Fluorescence Immunoassay ▿
    Article Snippet: Aliquots of 10 μl of test sera at a dilution of 1:10 in PBS were added to each well and incubated at 37°C for 15 min. .. The slides were then washed in PBS and incubated with 10 μl of fluorescein-conjugated anti-mouse IgG (MP Biomedicals, Irvine, CA) or fluorescein-conjugated anti-rat IgG (MP Biomedicals) diluted 1:100 in PBS containing 1% Evan's blue dye at 37°C for 15 min. After the slides were washed, specific fluorescence was analyzed using a UV epifluorescence microscope. .. The complete nucleotide sequence of the S gene cloned from the MHV-S strain has been submitted to DDBJ/EMBL/GenBank under accession no. .

    Microscopy:

    Article Title: Simultaneous Detection of Antibodies to Mouse Hepatitis Virus Recombinant Structural Proteins by a Microsphere-Based Multiplex Fluorescence Immunoassay ▿
    Article Snippet: Aliquots of 10 μl of test sera at a dilution of 1:10 in PBS were added to each well and incubated at 37°C for 15 min. .. The slides were then washed in PBS and incubated with 10 μl of fluorescein-conjugated anti-mouse IgG (MP Biomedicals, Irvine, CA) or fluorescein-conjugated anti-rat IgG (MP Biomedicals) diluted 1:100 in PBS containing 1% Evan's blue dye at 37°C for 15 min. After the slides were washed, specific fluorescence was analyzed using a UV epifluorescence microscope. .. The complete nucleotide sequence of the S gene cloned from the MHV-S strain has been submitted to DDBJ/EMBL/GenBank under accession no. .

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  • 90
    Valiant anti rat igg antibodies
    Association of SV40 Vp1 with cellular/viral (co)chaperones during SV40 infection. (A) The RIPA lysate prepared from 10 7 SV40-infected TC7 cells at 50 h postinfection was immunoprecipitated with affinity-purified anti-Vp1 <t>IgG</t> and analyzed for the presence of LT, Hsc70, Hsp70, or PDI by Western blotting. As a control, the same number of uninfected COS-7 cells was also analyzed. For input, 1/480 of the lysate used for immunoreactions was loaded. PDI and an immunoglobulin heavy chain (Ig) were detected with anti-PDI antibody (arrow). (B) The RIPA lysate from 10 6 infected cells was immunoprecipitated with antibodies against β-Gal, Hsc70, Hsp70, a unique LT epitope, a unique ST epitope, and Hsp40, and the IPs were probed for Vp1 by Western blotting. One-thousandth of the input lysate was analyzed for Vp1 in the same blot. (C) The Sol fraction from 10 6 infected cells, which contains endogenous and infection-based Vp1, was mixed with two recombinant Vp1 proteins, Vp1ΔC58-His6 pentamer (ΔC) and GST-Vp1C69 (C69). After IP with antibodies against β-Gal, Hsc70, Hsp70, Hsp40, a unique LT epitope, a unique ST epitope, and PDI, the IPs and 1/1,000 of the inputs were analyzed by anti-Vp1 Western blotting. The reactions shown in lanes 4, 5, and 10, to 12 were performed using <t>TrueBlot</t> anti-mouse beads. (D) The Csk fraction prepared from 2.5 × 10 6 infected cells was immunoprecipitated with antibodies against β-Gal, Hsc70, or a unique LT epitope, and Vp1 in the IPs was analyzed by anti-Vp1 Western blotting. Vp1 in 1/1,000 of the input was also analyzed. (E) The Sol fraction from 10 4 infected cells or the Csk fraction from 2.5 × 10 4 infected cells was analyzed for Hsc70, Hsp70, and LT by Western blotting.
    Anti Rat Igg Antibodies, supplied by Valiant, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti rat igg antibodies/product/Valiant
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti rat igg antibodies - by Bioz Stars, 2021-04
    90/100 stars
      Buy from Supplier

    Image Search Results


    Association of SV40 Vp1 with cellular/viral (co)chaperones during SV40 infection. (A) The RIPA lysate prepared from 10 7 SV40-infected TC7 cells at 50 h postinfection was immunoprecipitated with affinity-purified anti-Vp1 IgG and analyzed for the presence of LT, Hsc70, Hsp70, or PDI by Western blotting. As a control, the same number of uninfected COS-7 cells was also analyzed. For input, 1/480 of the lysate used for immunoreactions was loaded. PDI and an immunoglobulin heavy chain (Ig) were detected with anti-PDI antibody (arrow). (B) The RIPA lysate from 10 6 infected cells was immunoprecipitated with antibodies against β-Gal, Hsc70, Hsp70, a unique LT epitope, a unique ST epitope, and Hsp40, and the IPs were probed for Vp1 by Western blotting. One-thousandth of the input lysate was analyzed for Vp1 in the same blot. (C) The Sol fraction from 10 6 infected cells, which contains endogenous and infection-based Vp1, was mixed with two recombinant Vp1 proteins, Vp1ΔC58-His6 pentamer (ΔC) and GST-Vp1C69 (C69). After IP with antibodies against β-Gal, Hsc70, Hsp70, Hsp40, a unique LT epitope, a unique ST epitope, and PDI, the IPs and 1/1,000 of the inputs were analyzed by anti-Vp1 Western blotting. The reactions shown in lanes 4, 5, and 10, to 12 were performed using TrueBlot anti-mouse beads. (D) The Csk fraction prepared from 2.5 × 10 6 infected cells was immunoprecipitated with antibodies against β-Gal, Hsc70, or a unique LT epitope, and Vp1 in the IPs was analyzed by anti-Vp1 Western blotting. Vp1 in 1/1,000 of the input was also analyzed. (E) The Sol fraction from 10 4 infected cells or the Csk fraction from 2.5 × 10 4 infected cells was analyzed for Hsc70, Hsp70, and LT by Western blotting.

    Journal: Journal of Virology

    Article Title: Association of Simian Virus 40 Vp1 with 70-Kilodalton Heat Shock Proteins and Viral Tumor Antigens ▿

    doi: 10.1128/JVI.00844-08

    Figure Lengend Snippet: Association of SV40 Vp1 with cellular/viral (co)chaperones during SV40 infection. (A) The RIPA lysate prepared from 10 7 SV40-infected TC7 cells at 50 h postinfection was immunoprecipitated with affinity-purified anti-Vp1 IgG and analyzed for the presence of LT, Hsc70, Hsp70, or PDI by Western blotting. As a control, the same number of uninfected COS-7 cells was also analyzed. For input, 1/480 of the lysate used for immunoreactions was loaded. PDI and an immunoglobulin heavy chain (Ig) were detected with anti-PDI antibody (arrow). (B) The RIPA lysate from 10 6 infected cells was immunoprecipitated with antibodies against β-Gal, Hsc70, Hsp70, a unique LT epitope, a unique ST epitope, and Hsp40, and the IPs were probed for Vp1 by Western blotting. One-thousandth of the input lysate was analyzed for Vp1 in the same blot. (C) The Sol fraction from 10 6 infected cells, which contains endogenous and infection-based Vp1, was mixed with two recombinant Vp1 proteins, Vp1ΔC58-His6 pentamer (ΔC) and GST-Vp1C69 (C69). After IP with antibodies against β-Gal, Hsc70, Hsp70, Hsp40, a unique LT epitope, a unique ST epitope, and PDI, the IPs and 1/1,000 of the inputs were analyzed by anti-Vp1 Western blotting. The reactions shown in lanes 4, 5, and 10, to 12 were performed using TrueBlot anti-mouse beads. (D) The Csk fraction prepared from 2.5 × 10 6 infected cells was immunoprecipitated with antibodies against β-Gal, Hsc70, or a unique LT epitope, and Vp1 in the IPs was analyzed by anti-Vp1 Western blotting. Vp1 in 1/1,000 of the input was also analyzed. (E) The Sol fraction from 10 4 infected cells or the Csk fraction from 2.5 × 10 4 infected cells was analyzed for Hsc70, Hsp70, and LT by Western blotting.

    Article Snippet: For secondary antibodies, horseradish peroxidase-conjugated anti-rabbit, anti-mouse, and anti-rat IgG antibodies were bought from MP Biomedicals, and TrueBlot horseradish peroxidase-conjugated anti-rabbit antibody was bought from eBioscience.

    Techniques: Infection, Immunoprecipitation, Affinity Purification, Western Blot, Recombinant