vimentin  (Valiant)

 
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    Name:
    Anti human vimentin goat Liquid
    Description:
    Product is the lyophilized powder of goat antiserum to human uromucoid Tamm Horsfall glycoprotein and buffer salts
    Catalog Number:
    08657941
    Price:
    387.1
    Category:
    Life Sciences Antibodies Primary Antibodies
    Applications:
    Immunoassays
    Size:
    2 mL
    Buy from Supplier


    Structured Review

    Valiant vimentin
    Immunoreactivity of cultured retinal microglia from CX3CR1 +/GFP transgenic mice. Retinal microglia isolated from CX3CR1 +/GFP transgenic mice exhibited GFP fluorescence (A) as driven by the microglial-specific CX3CR1 promoter. Cultured cells were immunoreactive for classic microglial markers Iba-1 (B), CD11b (C), F4/80 (D), and CD68(E). The cells were immunonegative for other macroglial markers such as GFAP (F), <t>vimentin</t> (G), and neuronal markers, such as NeuN (H). Scale bar = 100 µm.
    Product is the lyophilized powder of goat antiserum to human uromucoid Tamm Horsfall glycoprotein and buffer salts
    https://www.bioz.com/result/vimentin/product/Valiant
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vimentin - by Bioz Stars, 2021-05
    85/100 stars

    Images

    1) Product Images from "Microglia in the Mouse Retina Alter the Structure and Function of Retinal Pigmented Epithelial Cells: A Potential Cellular Interaction Relevant to AMD"

    Article Title: Microglia in the Mouse Retina Alter the Structure and Function of Retinal Pigmented Epithelial Cells: A Potential Cellular Interaction Relevant to AMD

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0007945

    Immunoreactivity of cultured retinal microglia from CX3CR1 +/GFP transgenic mice. Retinal microglia isolated from CX3CR1 +/GFP transgenic mice exhibited GFP fluorescence (A) as driven by the microglial-specific CX3CR1 promoter. Cultured cells were immunoreactive for classic microglial markers Iba-1 (B), CD11b (C), F4/80 (D), and CD68(E). The cells were immunonegative for other macroglial markers such as GFAP (F), vimentin (G), and neuronal markers, such as NeuN (H). Scale bar = 100 µm.
    Figure Legend Snippet: Immunoreactivity of cultured retinal microglia from CX3CR1 +/GFP transgenic mice. Retinal microglia isolated from CX3CR1 +/GFP transgenic mice exhibited GFP fluorescence (A) as driven by the microglial-specific CX3CR1 promoter. Cultured cells were immunoreactive for classic microglial markers Iba-1 (B), CD11b (C), F4/80 (D), and CD68(E). The cells were immunonegative for other macroglial markers such as GFAP (F), vimentin (G), and neuronal markers, such as NeuN (H). Scale bar = 100 µm.

    Techniques Used: Cell Culture, Transgenic Assay, Mouse Assay, Isolation, Fluorescence

    Related Articles

    Blocking Assay:

    Article Title: Microglia in the Mouse Retina Alter the Structure and Function of Retinal Pigmented Epithelial Cells: A Potential Cellular Interaction Relevant to AMD
    Article Snippet: Immunohistochemistry Cultured cells and retinal tissue for immunohistochemical analyses were fixed (30 minutes for cultured cells and 4 hours for retinal tissue) in 4% paraformaldehyde in PBS, washed with phosphate buffered saline containing 0.25% Tween 20 (PBST), and then permeabilized with Triton-X (0.25% for cultured cells, 1% for tissues)(Sigma, St. Louis, MO) for 30 minutes. .. Cells or tissues were placed in a blocking solution (USB, Rodeo Blocker, Cleveland, OH) for 30 minutes, and incubated overnight with primary antibodies to the following antigens: CD11b, 1∶200; F4/80, 1∶200; CD68, 1∶200 (all from AbD Serotec, Raleigh, NC); ionized calcium binding adaptor molecule-1 (Iba1), 1∶500 (Wako, Richmond, VA); GFAP, 1∶200; Alexa Fluor 633-conjugated Phalloidin, 1∶100; zonula-occludins-1 (ZO-1), 1∶200 (all from Invitrogen, Carlsbad, CA); NeuN, 1∶200 (Millipore, Billerica, MA); vimentin, 1∶200 (MP Biomedicals, Solon, OH); CD31 [P2B1], 1∶100 (Development Studies Hybridoma Bank, Iowa City, IO). .. After washing, secondary antibodies, which were conjugated to either Alexa-488 or Alexa-568 (Invitrogen, Carlsbad, CA), were added at a 1∶200 dilution and incubated for 1–2 hrs.

    Incubation:

    Article Title: Microglia in the Mouse Retina Alter the Structure and Function of Retinal Pigmented Epithelial Cells: A Potential Cellular Interaction Relevant to AMD
    Article Snippet: Immunohistochemistry Cultured cells and retinal tissue for immunohistochemical analyses were fixed (30 minutes for cultured cells and 4 hours for retinal tissue) in 4% paraformaldehyde in PBS, washed with phosphate buffered saline containing 0.25% Tween 20 (PBST), and then permeabilized with Triton-X (0.25% for cultured cells, 1% for tissues)(Sigma, St. Louis, MO) for 30 minutes. .. Cells or tissues were placed in a blocking solution (USB, Rodeo Blocker, Cleveland, OH) for 30 minutes, and incubated overnight with primary antibodies to the following antigens: CD11b, 1∶200; F4/80, 1∶200; CD68, 1∶200 (all from AbD Serotec, Raleigh, NC); ionized calcium binding adaptor molecule-1 (Iba1), 1∶500 (Wako, Richmond, VA); GFAP, 1∶200; Alexa Fluor 633-conjugated Phalloidin, 1∶100; zonula-occludins-1 (ZO-1), 1∶200 (all from Invitrogen, Carlsbad, CA); NeuN, 1∶200 (Millipore, Billerica, MA); vimentin, 1∶200 (MP Biomedicals, Solon, OH); CD31 [P2B1], 1∶100 (Development Studies Hybridoma Bank, Iowa City, IO). .. After washing, secondary antibodies, which were conjugated to either Alexa-488 or Alexa-568 (Invitrogen, Carlsbad, CA), were added at a 1∶200 dilution and incubated for 1–2 hrs.

    Binding Assay:

    Article Title: Microglia in the Mouse Retina Alter the Structure and Function of Retinal Pigmented Epithelial Cells: A Potential Cellular Interaction Relevant to AMD
    Article Snippet: Immunohistochemistry Cultured cells and retinal tissue for immunohistochemical analyses were fixed (30 minutes for cultured cells and 4 hours for retinal tissue) in 4% paraformaldehyde in PBS, washed with phosphate buffered saline containing 0.25% Tween 20 (PBST), and then permeabilized with Triton-X (0.25% for cultured cells, 1% for tissues)(Sigma, St. Louis, MO) for 30 minutes. .. Cells or tissues were placed in a blocking solution (USB, Rodeo Blocker, Cleveland, OH) for 30 minutes, and incubated overnight with primary antibodies to the following antigens: CD11b, 1∶200; F4/80, 1∶200; CD68, 1∶200 (all from AbD Serotec, Raleigh, NC); ionized calcium binding adaptor molecule-1 (Iba1), 1∶500 (Wako, Richmond, VA); GFAP, 1∶200; Alexa Fluor 633-conjugated Phalloidin, 1∶100; zonula-occludins-1 (ZO-1), 1∶200 (all from Invitrogen, Carlsbad, CA); NeuN, 1∶200 (Millipore, Billerica, MA); vimentin, 1∶200 (MP Biomedicals, Solon, OH); CD31 [P2B1], 1∶100 (Development Studies Hybridoma Bank, Iowa City, IO). .. After washing, secondary antibodies, which were conjugated to either Alexa-488 or Alexa-568 (Invitrogen, Carlsbad, CA), were added at a 1∶200 dilution and incubated for 1–2 hrs.

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  • News
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  • 85
    Valiant vimentin
    Immunoreactivity of cultured retinal microglia from CX3CR1 +/GFP transgenic mice. Retinal microglia isolated from CX3CR1 +/GFP transgenic mice exhibited GFP fluorescence (A) as driven by the microglial-specific CX3CR1 promoter. Cultured cells were immunoreactive for classic microglial markers Iba-1 (B), CD11b (C), F4/80 (D), and CD68(E). The cells were immunonegative for other macroglial markers such as GFAP (F), <t>vimentin</t> (G), and neuronal markers, such as NeuN (H). Scale bar = 100 µm.
    Vimentin, supplied by Valiant, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vimentin/product/Valiant
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vimentin - by Bioz Stars, 2021-05
    85/100 stars
      Buy from Supplier

    Image Search Results


    Immunoreactivity of cultured retinal microglia from CX3CR1 +/GFP transgenic mice. Retinal microglia isolated from CX3CR1 +/GFP transgenic mice exhibited GFP fluorescence (A) as driven by the microglial-specific CX3CR1 promoter. Cultured cells were immunoreactive for classic microglial markers Iba-1 (B), CD11b (C), F4/80 (D), and CD68(E). The cells were immunonegative for other macroglial markers such as GFAP (F), vimentin (G), and neuronal markers, such as NeuN (H). Scale bar = 100 µm.

    Journal: PLoS ONE

    Article Title: Microglia in the Mouse Retina Alter the Structure and Function of Retinal Pigmented Epithelial Cells: A Potential Cellular Interaction Relevant to AMD

    doi: 10.1371/journal.pone.0007945

    Figure Lengend Snippet: Immunoreactivity of cultured retinal microglia from CX3CR1 +/GFP transgenic mice. Retinal microglia isolated from CX3CR1 +/GFP transgenic mice exhibited GFP fluorescence (A) as driven by the microglial-specific CX3CR1 promoter. Cultured cells were immunoreactive for classic microglial markers Iba-1 (B), CD11b (C), F4/80 (D), and CD68(E). The cells were immunonegative for other macroglial markers such as GFAP (F), vimentin (G), and neuronal markers, such as NeuN (H). Scale bar = 100 µm.

    Article Snippet: Cells or tissues were placed in a blocking solution (USB, Rodeo Blocker, Cleveland, OH) for 30 minutes, and incubated overnight with primary antibodies to the following antigens: CD11b, 1∶200; F4/80, 1∶200; CD68, 1∶200 (all from AbD Serotec, Raleigh, NC); ionized calcium binding adaptor molecule-1 (Iba1), 1∶500 (Wako, Richmond, VA); GFAP, 1∶200; Alexa Fluor 633-conjugated Phalloidin, 1∶100; zonula-occludins-1 (ZO-1), 1∶200 (all from Invitrogen, Carlsbad, CA); NeuN, 1∶200 (Millipore, Billerica, MA); vimentin, 1∶200 (MP Biomedicals, Solon, OH); CD31 [P2B1], 1∶100 (Development Studies Hybridoma Bank, Iowa City, IO).

    Techniques: Cell Culture, Transgenic Assay, Mouse Assay, Isolation, Fluorescence