rabbit anti sheep immunoglobulin g  (Valiant)

 
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    Name:
    Anti sheep IgG whole molecule affinity purified rabbit antibody fluorescein conjugated
    Description:
    Product is fluorescein 5 isothiocyanate FITC Isomer I conjugated rabbit affinity purified antibody to sheep IgG whole molecule and buffer salts
    Catalog Number:
    0857005
    Price:
    198.55
    Category:
    Life Sciences Antibodies Secondary Antibodies
    Applications:
    Immunoassays, Immunohistochemistry (Paraffin; Acetone-Fixed; 4% PFA Frozen), Immunofluorescence assays (IFA), Flow Cytometry (FACS)
    Size:
    2 mg
    Buy from Supplier


    Structured Review

    Valiant rabbit anti sheep immunoglobulin g
    Anti sheep IgG whole molecule affinity purified rabbit antibody fluorescein conjugated
    Product is fluorescein 5 isothiocyanate FITC Isomer I conjugated rabbit affinity purified antibody to sheep IgG whole molecule and buffer salts
    https://www.bioz.com/result/rabbit anti sheep immunoglobulin g/product/Valiant
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti sheep immunoglobulin g - by Bioz Stars, 2021-05
    91/100 stars

    Images

    1) Product Images from "Deletion of inositol-requiring enzyme-1α in podocytes disrupts glomerular capillary integrity and autophagy"

    Article Title: Deletion of inositol-requiring enzyme-1α in podocytes disrupts glomerular capillary integrity and autophagy

    Journal: Molecular Biology of the Cell

    doi: 10.1091/mbc.E16-12-0828

    Podocyte IRE1α deletion result in a reduction of WT1 and synaptopodin. (A–C) Kidney sections from 9-mo-old mice were stained with antibodies to WT1 (A), synaptopodin (B), and podocalyxin (C). Staining with nonimmune IgG is also presented (negative controls). Scale bars, 50 μm. (D, E) The number of WT1-positive nuclei per glomerulus (determined by colocalization with Hoechst nuclear stain; not shown) was assessed by visual counting. WT1 counts per glomerulus were comparable, but when expressed per 1000 µm 2 of glomerular area, WT1 counts were significantly lower in M Cre mice (* p = 4.6 × 10 −5 ); 32 M + glomeruli from three mice and 48 M Cre glomeruli from three mice. (F) Synaptopodin-stained glomeruli were enlarged in M Cre mice (* p = 0.024). (G) Quantification of synaptopodin immunofluorescence per unit glomerular area showed that intensity was lower in M Cre mice (* p = 0.046). For F and G, 74 M + glomeruli from three mice and 69 M Cre glomeruli from three mice. (H) Podocalyxin-stained glomeruli were enlarged in M Cre mice (* p = 0.0013). (I) Podocalyxin fluorescence per glomerulus and fluorescence per unit glomerular area were comparable in both groups. For H and I, 58 M + glomeruli from three mice and 54 M Cre glomeruli from three mice.
    Figure Legend Snippet: Podocyte IRE1α deletion result in a reduction of WT1 and synaptopodin. (A–C) Kidney sections from 9-mo-old mice were stained with antibodies to WT1 (A), synaptopodin (B), and podocalyxin (C). Staining with nonimmune IgG is also presented (negative controls). Scale bars, 50 μm. (D, E) The number of WT1-positive nuclei per glomerulus (determined by colocalization with Hoechst nuclear stain; not shown) was assessed by visual counting. WT1 counts per glomerulus were comparable, but when expressed per 1000 µm 2 of glomerular area, WT1 counts were significantly lower in M Cre mice (* p = 4.6 × 10 −5 ); 32 M + glomeruli from three mice and 48 M Cre glomeruli from three mice. (F) Synaptopodin-stained glomeruli were enlarged in M Cre mice (* p = 0.024). (G) Quantification of synaptopodin immunofluorescence per unit glomerular area showed that intensity was lower in M Cre mice (* p = 0.046). For F and G, 74 M + glomeruli from three mice and 69 M Cre glomeruli from three mice. (H) Podocalyxin-stained glomeruli were enlarged in M Cre mice (* p = 0.0013). (I) Podocalyxin fluorescence per glomerulus and fluorescence per unit glomerular area were comparable in both groups. For H and I, 58 M + glomeruli from three mice and 54 M Cre glomeruli from three mice.

    Techniques Used: Mouse Assay, Staining, Immunofluorescence, Fluorescence

    2) Product Images from "The phosphoinositide-binding protein p40phox activates the NADPH oxidase during Fc?IIA receptor-induced phagocytosis"

    Article Title: The phosphoinositide-binding protein p40phox activates the NADPH oxidase during Fc?IIA receptor-induced phagocytosis

    Journal: The Journal of Experimental Medicine

    doi: 10.1084/jem.20052085

    Expression of p40 phox mutants in COS phox FcγR cells and effect on IgG–sheep RBC–elicited NADPH oxidase activity. Data shown is representative of at least three independent experiments. (A) Immunoblot of cell lysates from COS phox FcγR cells transfected with 0.67 μg of either empty pRK5 or pRK5 containing cDNAs for either wild-type or mutant p40 phox . Blots were probed with antibodies for p40 phox , p47 phox , and p67 phox . (B) COS phox FcγR cells were transfected as in A and incubated with IgG-RBCs in the presence of NBT for 30 min at 37°C. The percentage of cells with NBT + phagosomes is shown as the mean ± SD ( n = 4 except for W207R/D289A, where n = 3). (C) COS phox FcγR cells were transfected as in A for expression of YFP-tagged wild-type or mutant derivatives of p40 phox as indicated or a YFP-tagged PX domain of p40 phox and incubated with IgG–sheep RBCs or with IgG latex beads (*) without or with 50 nM wortmannin, followed by confocal microscopy. Individual phagosomes were scored for either the presence (black bars) or absence of YFP-p40 phox or YFP-p40PX translocation. The number of phagosomes scored for each construct is also shown. Data was collected from two to four independent experiments.
    Figure Legend Snippet: Expression of p40 phox mutants in COS phox FcγR cells and effect on IgG–sheep RBC–elicited NADPH oxidase activity. Data shown is representative of at least three independent experiments. (A) Immunoblot of cell lysates from COS phox FcγR cells transfected with 0.67 μg of either empty pRK5 or pRK5 containing cDNAs for either wild-type or mutant p40 phox . Blots were probed with antibodies for p40 phox , p47 phox , and p67 phox . (B) COS phox FcγR cells were transfected as in A and incubated with IgG-RBCs in the presence of NBT for 30 min at 37°C. The percentage of cells with NBT + phagosomes is shown as the mean ± SD ( n = 4 except for W207R/D289A, where n = 3). (C) COS phox FcγR cells were transfected as in A for expression of YFP-tagged wild-type or mutant derivatives of p40 phox as indicated or a YFP-tagged PX domain of p40 phox and incubated with IgG–sheep RBCs or with IgG latex beads (*) without or with 50 nM wortmannin, followed by confocal microscopy. Individual phagosomes were scored for either the presence (black bars) or absence of YFP-p40 phox or YFP-p40PX translocation. The number of phagosomes scored for each construct is also shown. Data was collected from two to four independent experiments.

    Techniques Used: Expressing, Activity Assay, Transfection, Mutagenesis, Incubation, Confocal Microscopy, Translocation Assay, Construct

    Expression of p40 phox in COS phox FcγR cells and FcγR-elicited NADPH oxidase activity. Data shown is representative of at least three independent experiments. (A) Immunoblots of human neutrophil and COS7 cell lysates (10 μg protein per lane) probed with antibodies for p40 phox , p47 phox , and p67 phox . COS phox FcγR cells were transfected with either a stable p40 phox transgene or with varying amounts of p40pRK5 for transient expression as indicated. (B) IgG-RBC–elicited NADPH oxidase activity in COS phox FcγR cells expressing p40 phox . Multiple formazan-stained phagosomes (arrows) in COS phox FcγR transfected with 0.05 μg p40pRK5 (representative photomicrograph; bar, 30 μm). Similar numbers of formazan-stained phagosomes were present in COS phox FcγR cells transfected with larger amounts of plasmid or expressing p40 phox from a stable transgene. (C) Flow cytometry analysis of COSFcγR cell lines incubated with Fc OxyBURST Green–opsonized zymosan for 30 min. Fluorescence intensity is shown on the x axis.
    Figure Legend Snippet: Expression of p40 phox in COS phox FcγR cells and FcγR-elicited NADPH oxidase activity. Data shown is representative of at least three independent experiments. (A) Immunoblots of human neutrophil and COS7 cell lysates (10 μg protein per lane) probed with antibodies for p40 phox , p47 phox , and p67 phox . COS phox FcγR cells were transfected with either a stable p40 phox transgene or with varying amounts of p40pRK5 for transient expression as indicated. (B) IgG-RBC–elicited NADPH oxidase activity in COS phox FcγR cells expressing p40 phox . Multiple formazan-stained phagosomes (arrows) in COS phox FcγR transfected with 0.05 μg p40pRK5 (representative photomicrograph; bar, 30 μm). Similar numbers of formazan-stained phagosomes were present in COS phox FcγR cells transfected with larger amounts of plasmid or expressing p40 phox from a stable transgene. (C) Flow cytometry analysis of COSFcγR cell lines incubated with Fc OxyBURST Green–opsonized zymosan for 30 min. Fluorescence intensity is shown on the x axis.

    Techniques Used: Expressing, Activity Assay, Western Blot, Transfection, Staining, Plasmid Preparation, Flow Cytometry, Cytometry, Incubation, Fluorescence

    Related Articles

    other:

    Article Title: Deletion of inositol-requiring enzyme-1α in podocytes disrupts glomerular capillary integrity and autophagy
    Article Snippet: Fluorescein isothiocyanate (FITC)-conjugated goat anti-mouse complement C3 antibody (Cappel 55500) and rabbit anti-sheep immunoglobulin G (IgG; 0865205) were purchased from MP Biomedicals (Santa Ana, CA).

    Incubation:

    Article Title: Transmembrane pickets connect cyto-and pericellular-skeletons forming barriers to receptor engagement
    Article Snippet: To visualize the IgG, coverslips were stained with 0.1 μg/mL Cy3-conjugated donkey anti-human antibody (Jackson ImmunoResearch) for 5 min. .. For opsonisation of erythrocytes, 0.5 mg/mL of rabbit anti-sheep IgG (MP Biomedicals) was incubated at with 200 μL of a 1% solution of washed sheep erythrocytes (MP Biomedicals) for 2 h at 37 °C. .. For opsonisation, 200 μL of a suspension (1% solids in PBS) of polystyrene beads containing 2% divinylbenzene (1.5, 5, or 8 μm diameter; Bangs Laboratories) was incubated with human IgG (50, 10, or 5 μg/mL; Sigma) for 2 h at 37°C.

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  • 91
    Valiant rabbit anti sheep immunoglobulin g
    Podocyte IRE1α deletion result in a reduction of WT1 and synaptopodin. (A–C) Kidney sections from 9-mo-old mice were stained with antibodies to WT1 (A), synaptopodin (B), and podocalyxin (C). Staining with nonimmune <t>IgG</t> is also presented (negative controls). Scale bars, 50 μm. (D, E) The number of WT1-positive nuclei per glomerulus (determined by colocalization with Hoechst nuclear stain; not shown) was assessed by visual counting. WT1 counts per glomerulus were comparable, but when expressed per 1000 µm 2 of glomerular area, WT1 counts were significantly lower in M Cre mice (* p = 4.6 × 10 −5 ); 32 M + glomeruli from three mice and 48 M Cre glomeruli from three mice. (F) Synaptopodin-stained glomeruli were enlarged in M Cre mice (* p = 0.024). (G) Quantification of synaptopodin immunofluorescence per unit glomerular area showed that intensity was lower in M Cre mice (* p = 0.046). For F and G, 74 M + glomeruli from three mice and 69 M Cre glomeruli from three mice. (H) Podocalyxin-stained glomeruli were enlarged in M Cre mice (* p = 0.0013). (I) Podocalyxin fluorescence per glomerulus and fluorescence per unit glomerular area were comparable in both groups. For H and I, 58 M + glomeruli from three mice and 54 M Cre glomeruli from three mice.
    Rabbit Anti Sheep Immunoglobulin G, supplied by Valiant, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti sheep immunoglobulin g/product/Valiant
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti sheep immunoglobulin g - by Bioz Stars, 2021-05
    91/100 stars
      Buy from Supplier

    Image Search Results


    Podocyte IRE1α deletion result in a reduction of WT1 and synaptopodin. (A–C) Kidney sections from 9-mo-old mice were stained with antibodies to WT1 (A), synaptopodin (B), and podocalyxin (C). Staining with nonimmune IgG is also presented (negative controls). Scale bars, 50 μm. (D, E) The number of WT1-positive nuclei per glomerulus (determined by colocalization with Hoechst nuclear stain; not shown) was assessed by visual counting. WT1 counts per glomerulus were comparable, but when expressed per 1000 µm 2 of glomerular area, WT1 counts were significantly lower in M Cre mice (* p = 4.6 × 10 −5 ); 32 M + glomeruli from three mice and 48 M Cre glomeruli from three mice. (F) Synaptopodin-stained glomeruli were enlarged in M Cre mice (* p = 0.024). (G) Quantification of synaptopodin immunofluorescence per unit glomerular area showed that intensity was lower in M Cre mice (* p = 0.046). For F and G, 74 M + glomeruli from three mice and 69 M Cre glomeruli from three mice. (H) Podocalyxin-stained glomeruli were enlarged in M Cre mice (* p = 0.0013). (I) Podocalyxin fluorescence per glomerulus and fluorescence per unit glomerular area were comparable in both groups. For H and I, 58 M + glomeruli from three mice and 54 M Cre glomeruli from three mice.

    Journal: Molecular Biology of the Cell

    Article Title: Deletion of inositol-requiring enzyme-1α in podocytes disrupts glomerular capillary integrity and autophagy

    doi: 10.1091/mbc.E16-12-0828

    Figure Lengend Snippet: Podocyte IRE1α deletion result in a reduction of WT1 and synaptopodin. (A–C) Kidney sections from 9-mo-old mice were stained with antibodies to WT1 (A), synaptopodin (B), and podocalyxin (C). Staining with nonimmune IgG is also presented (negative controls). Scale bars, 50 μm. (D, E) The number of WT1-positive nuclei per glomerulus (determined by colocalization with Hoechst nuclear stain; not shown) was assessed by visual counting. WT1 counts per glomerulus were comparable, but when expressed per 1000 µm 2 of glomerular area, WT1 counts were significantly lower in M Cre mice (* p = 4.6 × 10 −5 ); 32 M + glomeruli from three mice and 48 M Cre glomeruli from three mice. (F) Synaptopodin-stained glomeruli were enlarged in M Cre mice (* p = 0.024). (G) Quantification of synaptopodin immunofluorescence per unit glomerular area showed that intensity was lower in M Cre mice (* p = 0.046). For F and G, 74 M + glomeruli from three mice and 69 M Cre glomeruli from three mice. (H) Podocalyxin-stained glomeruli were enlarged in M Cre mice (* p = 0.0013). (I) Podocalyxin fluorescence per glomerulus and fluorescence per unit glomerular area were comparable in both groups. For H and I, 58 M + glomeruli from three mice and 54 M Cre glomeruli from three mice.

    Article Snippet: Fluorescein isothiocyanate (FITC)-conjugated goat anti-mouse complement C3 antibody (Cappel 55500) and rabbit anti-sheep immunoglobulin G (IgG; 0865205) were purchased from MP Biomedicals (Santa Ana, CA).

    Techniques: Mouse Assay, Staining, Immunofluorescence, Fluorescence

    Expression of p40 phox mutants in COS phox FcγR cells and effect on IgG–sheep RBC–elicited NADPH oxidase activity. Data shown is representative of at least three independent experiments. (A) Immunoblot of cell lysates from COS phox FcγR cells transfected with 0.67 μg of either empty pRK5 or pRK5 containing cDNAs for either wild-type or mutant p40 phox . Blots were probed with antibodies for p40 phox , p47 phox , and p67 phox . (B) COS phox FcγR cells were transfected as in A and incubated with IgG-RBCs in the presence of NBT for 30 min at 37°C. The percentage of cells with NBT + phagosomes is shown as the mean ± SD ( n = 4 except for W207R/D289A, where n = 3). (C) COS phox FcγR cells were transfected as in A for expression of YFP-tagged wild-type or mutant derivatives of p40 phox as indicated or a YFP-tagged PX domain of p40 phox and incubated with IgG–sheep RBCs or with IgG latex beads (*) without or with 50 nM wortmannin, followed by confocal microscopy. Individual phagosomes were scored for either the presence (black bars) or absence of YFP-p40 phox or YFP-p40PX translocation. The number of phagosomes scored for each construct is also shown. Data was collected from two to four independent experiments.

    Journal: The Journal of Experimental Medicine

    Article Title: The phosphoinositide-binding protein p40phox activates the NADPH oxidase during Fc?IIA receptor-induced phagocytosis

    doi: 10.1084/jem.20052085

    Figure Lengend Snippet: Expression of p40 phox mutants in COS phox FcγR cells and effect on IgG–sheep RBC–elicited NADPH oxidase activity. Data shown is representative of at least three independent experiments. (A) Immunoblot of cell lysates from COS phox FcγR cells transfected with 0.67 μg of either empty pRK5 or pRK5 containing cDNAs for either wild-type or mutant p40 phox . Blots were probed with antibodies for p40 phox , p47 phox , and p67 phox . (B) COS phox FcγR cells were transfected as in A and incubated with IgG-RBCs in the presence of NBT for 30 min at 37°C. The percentage of cells with NBT + phagosomes is shown as the mean ± SD ( n = 4 except for W207R/D289A, where n = 3). (C) COS phox FcγR cells were transfected as in A for expression of YFP-tagged wild-type or mutant derivatives of p40 phox as indicated or a YFP-tagged PX domain of p40 phox and incubated with IgG–sheep RBCs or with IgG latex beads (*) without or with 50 nM wortmannin, followed by confocal microscopy. Individual phagosomes were scored for either the presence (black bars) or absence of YFP-p40 phox or YFP-p40PX translocation. The number of phagosomes scored for each construct is also shown. Data was collected from two to four independent experiments.

    Article Snippet: IgG-coated RBCs (IgG-RBC) were freshly prepared as described previously using sheep RBCs and rabbit anti–sheep RBC IgG (MP Biomedicals) ( ).

    Techniques: Expressing, Activity Assay, Transfection, Mutagenesis, Incubation, Confocal Microscopy, Translocation Assay, Construct

    Expression of p40 phox in COS phox FcγR cells and FcγR-elicited NADPH oxidase activity. Data shown is representative of at least three independent experiments. (A) Immunoblots of human neutrophil and COS7 cell lysates (10 μg protein per lane) probed with antibodies for p40 phox , p47 phox , and p67 phox . COS phox FcγR cells were transfected with either a stable p40 phox transgene or with varying amounts of p40pRK5 for transient expression as indicated. (B) IgG-RBC–elicited NADPH oxidase activity in COS phox FcγR cells expressing p40 phox . Multiple formazan-stained phagosomes (arrows) in COS phox FcγR transfected with 0.05 μg p40pRK5 (representative photomicrograph; bar, 30 μm). Similar numbers of formazan-stained phagosomes were present in COS phox FcγR cells transfected with larger amounts of plasmid or expressing p40 phox from a stable transgene. (C) Flow cytometry analysis of COSFcγR cell lines incubated with Fc OxyBURST Green–opsonized zymosan for 30 min. Fluorescence intensity is shown on the x axis.

    Journal: The Journal of Experimental Medicine

    Article Title: The phosphoinositide-binding protein p40phox activates the NADPH oxidase during Fc?IIA receptor-induced phagocytosis

    doi: 10.1084/jem.20052085

    Figure Lengend Snippet: Expression of p40 phox in COS phox FcγR cells and FcγR-elicited NADPH oxidase activity. Data shown is representative of at least three independent experiments. (A) Immunoblots of human neutrophil and COS7 cell lysates (10 μg protein per lane) probed with antibodies for p40 phox , p47 phox , and p67 phox . COS phox FcγR cells were transfected with either a stable p40 phox transgene or with varying amounts of p40pRK5 for transient expression as indicated. (B) IgG-RBC–elicited NADPH oxidase activity in COS phox FcγR cells expressing p40 phox . Multiple formazan-stained phagosomes (arrows) in COS phox FcγR transfected with 0.05 μg p40pRK5 (representative photomicrograph; bar, 30 μm). Similar numbers of formazan-stained phagosomes were present in COS phox FcγR cells transfected with larger amounts of plasmid or expressing p40 phox from a stable transgene. (C) Flow cytometry analysis of COSFcγR cell lines incubated with Fc OxyBURST Green–opsonized zymosan for 30 min. Fluorescence intensity is shown on the x axis.

    Article Snippet: IgG-coated RBCs (IgG-RBC) were freshly prepared as described previously using sheep RBCs and rabbit anti–sheep RBC IgG (MP Biomedicals) ( ).

    Techniques: Expressing, Activity Assay, Western Blot, Transfection, Staining, Plasmid Preparation, Flow Cytometry, Cytometry, Incubation, Fluorescence