spermidine  (Valiant)

 
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  • 86
    Name:
    Spermidine molecular biology reagent 99
    Description:
    Spermidine
    Catalog Number:
    0219485225
    Price:
    588.7
    Category:
    Life Sciences Biochemicals
    Size:
    25 g
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    Structured Review

    Valiant spermidine
    Spermidine molecular biology reagent 99
    Spermidine
    https://www.bioz.com/result/spermidine/product/Valiant
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    spermidine - by Bioz Stars, 2021-04
    86/100 stars

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    Related Articles

    other:

    Article Title: Legionella pneumophilaRequires Polyamines for Optimal Intracellular Growth ▿
    Article Snippet: BYE broth or DM containing cadaverine, putrescine, spermine, or spermidine (MP Biomedicals) was inoculated as follows.

    Concentration Assay:

    Article Title: Reduced local mutation density in regulatory DNA of cancer genomes is linked to DNA repair
    Article Snippet: To prepare nuclei, freshly grown cells were centrifuged at 500g for 5 minutes (4°C) in an Eppendorf Centrifuge 5810R, and washed in cold PBS (Cellgro/Mediatech Inc.). .. Cell pellets were resuspended in Buffer A (15 mM Tris-Cl pH 8.0, 15 mM NaCl, 60 mM KCl, 1 mM EDTA (Ambion/Life Technologies Corp) pH 8.0, 0.5 mM EGTA (Boston BioProducts) pH 8.0, 0.5 mM spermidine (MP Biomedicals, LLC) and 0.15 mM spermine (MP Biomedicals, LLC) to a final concentration of 2 × 106 cells/mL. .. Nuclei were obtained by dropwise addition of an equal volume of Buffer A containing 0.04% IGEPAL CA-630 (Sigma-Aldrich) to the cells, followed by incubation on ice for 10 min.

    Article Title: Legionella pneumophilaRequires Polyamines for Optimal Intracellular Growth ▿
    Article Snippet: L929 cells were prepared for infection and processed to obtain CFU/well counts as described above under “Effects of pharmacological inhibitors of polyamine synthesis on the intracellular growth of L. pneumophila ,” with the following changes. .. The FBS-free MEM used to add the bacterial inoculum and maintain the polyamine-treated cells during the infection period was supplemented with spermine and spermidine (both from MP Biomedicals and used at a final concentration of 100 μM). .. For wells infected with L. pneumophila pretreated with polyamines, the bacterial inoculum was prepared from strain JR32 grown to stationary phase in BYE broth supplemented with spermine and spermidine (both at a final concentration of 100 μM).

    Infection:

    Article Title: Legionella pneumophilaRequires Polyamines for Optimal Intracellular Growth ▿
    Article Snippet: L929 cells were prepared for infection and processed to obtain CFU/well counts as described above under “Effects of pharmacological inhibitors of polyamine synthesis on the intracellular growth of L. pneumophila ,” with the following changes. .. The FBS-free MEM used to add the bacterial inoculum and maintain the polyamine-treated cells during the infection period was supplemented with spermine and spermidine (both from MP Biomedicals and used at a final concentration of 100 μM). .. For wells infected with L. pneumophila pretreated with polyamines, the bacterial inoculum was prepared from strain JR32 grown to stationary phase in BYE broth supplemented with spermine and spermidine (both at a final concentration of 100 μM).

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  • 86
    Valiant spermidine
    Spermidine, supplied by Valiant, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/spermidine/product/Valiant
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    spermidine - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

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